Explanipedia

Actin nano-architecture of phagocytic podosomes Open

Takashi Watanabe, Ana T. Nogueira, Megan Kern, Jesse Aaron, Aaron B. Taylor , et al. · 2025

Author Correction: Optogenetic control of cofilin and αTAT in living cells using Z-lock Open

Orrin J. Stone, Neha Pankow, Bei Liu, Ved P. Sharma, Robert J. Eddy , et al. · 2025

Optogenetic control of cofilin and αTAT in living cells using Z-lock Open

Orrin J. Stone, Neha Pankow, Bei Liu, Ved P. Sharma, Robert J. Eddy , et al. · 2025

Deep learning enables structured illumination microscopy with low light levels and enhanced speed Open

Luhong Jin, Bei Liu, Fenqiang Zhao, Stephen Hahn, Bowei Dong , et al. · 2025

Regulation of local GTP availability controls RAC1 activity and cell invasion Open

Anna Bianchi, David W. Wolff, Daniel J. Marston, Zhiyong Deng, Zhannan Han , et al. · 2025

Author Correction: Regulation of local GTP availability controls RAC1 activity and cell invasion Open

Anna Bianchi, David W. Wolff, Daniel J. Marston, Zhiyong Deng, Zhannan Han , et al. · 2025

Object Detection with Deep Learning for Rare Event Search in the GADGET II TPC Open

T. Wheeler, Saiprasad Ravishankar, C. Wrede, Amin Andalib, Ashwin Anthony , et al. · 2025

In the pursuit of identifying rare two-particle events within the GADGET II Time Projection Chamber (TPC), this paper presents a comprehensive approach for leveraging Convolutional Neural Networks (CNNs) and various data processing methods…

Software for lattice light-sheet imaging of FRET biosensors, illustrated with a new Rap1 biosensor Open

Ellen C. O’Shaughnessy, Orrin J. Stone, Paul K. LaFosse, Mihai L. Azoitei, Denis Tsygankov , et al. · 2025

Lattice light-sheet microscopy (LLSM) is valuable for its combination of reduced photobleaching and outstanding spatiotemporal resolution in 3D. Using LLSM to image biosensors in living cells could provide unprecedented visualization of ra…

Time-resolved cryo-EM (TR-EM) analysis of substrate polyubiquitination by the RING E3 anaphase-promoting complex/cyclosome (APC/C) Open

Tatyana Bodrug, Kaeli A. Welsh, Derek L. Bolhuis, Ethan PaulÐ°konis, Raquel C. Martinez‐Chacin , et al. · 2024

Live-cell biosensors based on the fluorescence lifetime of environment-sensing dyes Open

Brian P. Mehl, Pothiappan Vairaprakash, Li Li, Elizabeth Hinde, Christopher J. MacNevin , et al. · 2024

In this work, we examine the use of environment-sensitive fluorescent dyes in fluorescence lifetime imaging microscopy (FLIM) biosensors. We screened merocyanine dyes to find an optimal combination of environment-induced lifetime changes, …

Time-resolved cryo-EM (TR-EM) analysis of substrate polyubiquitination by the RING E3 anaphase-promoting complex/cyclosome (APC/C) Open

Tatyana Bodrug, Kaeli A. Welsh, Derek L. Bolhuis, Ethan Paulаkonis, Raquel C. Martinez‐Chacin , et al. · 2023

Celebrating the creative scientific life of Ken Jacobson Open

Klaus M. Hahn, Michelle S. Itano, Leslie M. Loew, Eric A. Vitriol · 2023

Rho MultiBinder, a fluorescent biosensor that reports the activity of multiple GTPases Open

Frederico M. Pimenta, Jaewon Huh, Bryan B. Guzmán, Diepreye Amanah, Daniel J. Marston , et al. · 2023

Abstract 1513: New approaches to study conformational changes of individual molecules in living cells Open

Klaus M. Hahn · 2023

Spatial models of pattern formation during phagocytosis Open

J. Cody Herron, Shiqiong Hu, Bei Liu, Takashi Watanabe, Klaus M. Hahn , et al. · 2022

Phagocytosis, the biological process in which cells ingest large particles such as bacteria, is a key component of the innate immune response. Fcγ receptor (FcγR)-mediated phagocytosis is initiated when these receptors are activated after …

Actin nano-architecture of phagocytic podosomes Open

J. Cody Herron, Shiqiong Hu, Takashi Watanabe, Ana T. Nogueira, Bei Liu , et al. · 2022

Images used for Actin nano-architecture of phagocytic podosomes Open

J. Cody Herron, Shiqiong Hu, Takashi Watanabe, Klaus M. Hahn · 2022

Images used for Actin nano-architecture of phagocytic podosomes

Images used for Actin nano-architecture of phagocytic podosomes Open

J. Cody Herron, Shiqiong Hu, Takashi Watanabe, Klaus M. Hahn · 2022

Images used for Actin nano-architecture of phagocytic podosomes

Actin nano-architecture of phagocytic podosomes Open

J. Cody Herron, Shiqiong Hu, Takashi Watanabe, Ana T. Nogueira, Bei Liu , et al. · 2022

Podosomes are actin-enriched adhesion structures important for multiple cellular processes, including migration, bone remodeling, and phagocytosis. Here, we characterized the structure and organization of phagocytic podosomes using interfe…

Spatial Models of Pattern Formation During Phagocytosis Open

J. Cody Herron, Shiqiong Hu, Bei Liu, Takashi Watanabe, Klaus M. Hahn , et al. · 2022

Phagocytosis, the biological process in which cells ingest large particles such as bacteria, is a key component of the innate immune response. Fcγ receptor (FcγR)-mediated phagocytosis is initiated when these receptors are activated after …

Live cell biosensors based on the fluorescence lifetime of environment-sensing dyes Open

Brian P. Mehl, Li Li, Elizabeth Hinde, Bei Liu, Christopher J. MacNevin , et al. · 2022

Most biosensors used with fluorescence lifetime imaging (FLIM) have been based on fluorescence resonance energy transfer (FRET). Here we examined the capabilities of FLIM biosensors based on environment-sensing dyes. We screened merocyanin…

PKCθ-mediated serine/threonine phosphorylations of FAK govern adhesion and protrusion dynamics within the lamellipodia of migrating breast cancer cells Open

Lucie Chadelle, Jiaying Liu, Valérie Choesmel-Cadamuro, Andrei V. Karginov, Carine Froment , et al. · 2021

A multi-functional microfluidic device compatible with widefield and light sheet microscopy Open

Regan P. Moore, Ellen C. O’Shaughnessy, Yu Shi, Ana T. Nogueira, Katelyn M. Heath , et al. · 2021

FEP-based microfluidics enable diverse applications in light sheet microscopy.

Author Correction: Regulation of local GTP availability controls RAC1 activity and cell invasion Open

Anna Bianchi, David W. Wolff, Daniel J. Marston, Zhiyong Deng, Zhannan Han , et al. · 2021

Regulation of local GTP availability controls RAC1 activity and cell invasion Open

Anna Bianchi, David W. Wolff, Daniel J. Marston, Zhiyong Deng, Zhannan Han , et al. · 2021

Biosensors based on peptide exposure show single molecule conformations in live cells Open

Bei Liu, Orrin J. Stone, Michael Pablo, J. Cody Herron, Ana T. Nogueira , et al. · 2021

Correcting Artifacts in Ratiometric Biosensor Imaging; an Improved Approach for Dividing Noisy Signals Open

Daniel J. Marston, Scott D. Slattery, Klaus M. Hahn, Denis Tsygankov · 2021

The accuracy of biosensor ratio imaging is limited by signal/noise. Signals can be weak when biosensor concentrations must be limited to avoid cell perturbation. This can be especially problematic in imaging of low volume regions, e.g., al…

Vinculin Controls PTEN Protein Level by Maintaining the Interaction of the Adherens Junction Protein β-Catenin with the Scaffolding Protein MAGI-2 Open

M. Cecilia Subauste, Perihan Nalbant, Eileen D. Adamson, Klaus M. Hahn · 2021

PTEN is a frequently mutated tumor suppressor in malignancies. Interestingly, some malignancies exhibit undetectable PTEN protein without mutations or loss of PTEN mRNA. The cause(s) for this reduction in PTEN is unknown. Cancer cells freq…

Labelling and optical erasure of synaptic memory traces in the motor cortex Open

Akiko Hayashi‐Takagi, Sho Yagishita, Mayumi Nakamura, Fukutoshi Shirai, Yi Wu , et al. · 2021

Dendritic spines are the major loci of synaptic plasticity and are considered as possible structural correlates of memory. Nonetheless, systematic manipulation of specific subsets of spines in the cortex has been unattainable, and thus, th…

An optogenetic method for interrogating YAP1 and TAZ nuclear–cytoplasmic shuttling Open

Anna M. Dowbaj, Robert P. Jenkins, Daniel Williamson, John M. Heddleston, Alessandro Ciccarelli , et al. · 2021

The shuttling of transcription factors and transcriptional regulators into and out of the nucleus is central to the regulation of many biological processes. Here we describe a new method for studying the rates of nuclear entry and exit of …

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