Elly M. Hol
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TMIC-52. Clinical correlates of the diffuse glioma mechanical microenvironment - a systematic review Open
BACKGROUND Glioma progression and invasion is characterized by intricate tumor-microenvironment crosstalk. Numerous studies have shown influence of the mechanical properties of the tumor microenvironment, i.e. the mechanical microenvironme…
Mutations in <span><i>GFAP</i></span> Alter Early Lineage Commitment of Organoids Open
Glial fibrillary acidic protein (GFAP) is a type‐3 intermediate filament protein mainly expressed in astrocytes in the central nervous system. Mutations in GFAP cause Alexander disease (AxD), a rare and fatal neurological disorder. How exa…
View article: Neuroinflammation in long-term cognitive impairment after aneurysmal subarachnoid hemorrhage
Neuroinflammation in long-term cognitive impairment after aneurysmal subarachnoid hemorrhage Open
Background: Survivors of aneurysmal subarachnoid hemorrhage (aSAH) often have cognitive impairment, which may be caused by long-term inflammation. We aimed to determine whether long-term neuroinflammation or microstructural brain damage is…
View article: Non-Immune-Mediated, p27-Associated, Growth Inhibition of Glioblastoma by Class-II-Transactivator (CIITA)
Non-Immune-Mediated, p27-Associated, Growth Inhibition of Glioblastoma by Class-II-Transactivator (CIITA) Open
Background: Previous works have shown that the expression of Class-II-Transactivator (CIITA) in tumor cells reduces the growth of glioblastoma (GB) in animal models, but immune effects cannot solely explain this. Here, we searched for immu…
Aberrant neurodevelopment in human <span>iPS</span> cell‐derived models of Alexander disease Open
Alexander disease (AxD) is a rare and severe neurodegenerative disorder caused by mutations in glial fibrillary acidic protein (GFAP). While the exact disease mechanism remains unknown, previous studies suggest that mutant GFAP influences …
The <span>GFAP</span> proteoform puzzle: How to advance <span>GFAP</span> as a fluid biomarker in neurological diseases Open
Glial fibrillary acidic protein (GFAP) is a well‐established biomarker of reactive astrogliosis in the central nervous system because of its elevated levels following brain injury and various neurological disorders. The advent of ultra‐sen…
Early amyloid‐induced changes in microglia gene expression in male <span>APP</span> / <span>PS1</span> mice Open
Alzheimer's disease (AD) is a progressive neurodegenerative disease and the most common cause of dementia, characterized by deposition of extracellular amyloid‐beta (Aβ) aggregates and intraneuronal hyperphosphorylated Tau. Many AD risk ge…
GFAP-isoforms in the nervous system: Understanding the need for diversity Open
Glial fibrillary acidic protein (GFAP) is an intermediate filament (IF) protein expressed in specific types of glial cells in the nervous system. The expression of GFAP is highly regulated during brain development and in neurological disea…
View article: Limited Effects of Class II Transactivator-Based Immunotherapy in Murine and Human Glioblastoma
Limited Effects of Class II Transactivator-Based Immunotherapy in Murine and Human Glioblastoma Open
Background: The major histocompatibility complex type II is downregulated in glioblastoma (GB) due to the silencing of the major transcriptional regulator class II transactivator (CIITA). We investigated the pro-immunogenic potential of CI…
Performance testing of a novel GFAP ELISA for CSF and blood based matrices Open
Background Glial Fibrillary Acidic Protein (GFAP) and its isoforms are intermediate filament cytoskeletal proteins expressed in astrocytes. Reactive astrogliosis, hallmarked by an increase in GFAP expression, is part of the pathogenesis of…
Inflammation, Anti-inflammatory Interventions, and Post-stroke Cognitive Impairment: a Systematic Review and Meta-analysis of Human and Animal Studies Open
The pathophysiology and treatment of post-stroke cognitive impairment (PSCI) are not clear. Stroke triggers an inflammatory response, which might affect synapse function and cognitive status. We performed a systematic review and meta-analy…
Transcriptomic and morphological maturation of human astrocytes in cerebral organoids Open
Cerebral organoids (CerOrgs) derived from human induced pluripotent stem cells (iPSCs) are a valuable tool to study human astrocytes and their interaction with neurons and microglia. The timeline of astrocyte development and maturation in …
Safety and pharmacodynamic efficacy of eculizumab in aneurysmal subarachnoid hemorrhage (CLASH): A phase 2a randomized clinical trial Open
Introduction: Complement C5 antibodies reduce brain injury after experimental subarachnoid hemorrhage. Patients and methods: In this randomized, controlled, open-label, phase 2a clinical trial with blinded-outcome assessment, we included a…
Glial cell response and microthrombosis in aneurysmal subarachnoid hemorrhage patients: An autopsy study Open
Neuroinflammation and microthrombosis may be underlying mechanisms of brain injury after aneurysmal subarachnoid hemorrhage (aSAH), but they have not been studied in relation to each other. In postmortem brain tissue, we investigated neuro…
Corrigendum: Reactive astrogliosis in the era of single-cell transcriptomics Open
[This corrects the article DOI: 10.3389/fncel.2023.1173200.].
Reactive astrogliosis in the era of single-cell transcriptomics Open
Reactive astrogliosis is a reaction of astrocytes to disturbed homeostasis in the central nervous system (CNS), accompanied by changes in astrocyte numbers, morphology, and function. Reactive astrocytes are important in the onset and progr…
View article: <i>In vivo</i> imaging of cerebral glucose metabolism informs on subacute to chronic post-stroke tissue status – A pilot study combining PET and deuterium metabolic imaging
<i>In vivo</i> imaging of cerebral glucose metabolism informs on subacute to chronic post-stroke tissue status – A pilot study combining PET and deuterium metabolic imaging Open
Recanalization therapy after acute ischemic stroke enables restoration of cerebral perfusion. However, a significant subset of patients has poor outcome, which may be caused by disruption of cerebral energy metabolism. To assess changes in…
The Role of Astrocytes in Synapse Loss in Alzheimer's Disease: A Systematic Review Open
Alzheimer's disease (AD) is the most common cause of dementia, affecting 35 million people worldwide. One pathological feature of progressing AD is the loss of synapses. This is the strongest correlate of cognitive decline. Astrocytes, as …
Exposure to the Amino Acids Histidine, Lysine, and Threonine Reduces mTOR Activity and Affects Neurodevelopment in a Human Cerebral Organoid Model Open
Evidence of the impact of nutrition on human brain development is compelling. Previous in vitro and in vivo results show that three specific amino acids, histidine, lysine, and threonine, synergistically inhibit mTOR activity and behavior.…
Calcium signaling in individual APP/PS1 mouse dentate gyrus astrocytes increases <i>ex vivo</i> with Aβ pathology and age without affecting astrocyte network activity Open
Astrocytes are critical for healthy brain function. In Alzheimer’s disease, astrocytes become reactive, which affects their signaling properties. Here, we measured spontaneous calcium transients ex vivo in hippocampal astrocytes in brain s…