M. Jake Pushie
YOU?
Author Swipe
View article: X-ray fluorescence mapping of brain tissue reveals the profound extent of trace element dysregulation in stroke pathophysiology
X-ray fluorescence mapping of brain tissue reveals the profound extent of trace element dysregulation in stroke pathophysiology Open
The brain is a privileged organ with regard to its trace element composition and maintains a robust barrier system to sequester this specialized environment from the rest of the body and the vascular system. Stroke is caused by loss of ade…
View article: Neuronal and Astrocyte Insulin-like Growth Factor-1 Signaling Differentially Modulates Ischemic Stroke Damage
Neuronal and Astrocyte Insulin-like Growth Factor-1 Signaling Differentially Modulates Ischemic Stroke Damage Open
Ischemic stroke is a leading cause of death and disability, as therapeutic options for mitigating the long-term deficits precipitated by the event remain limited. Acute administration of the neuroendocrine modulator insulin-like growth fac…
View article: Alzheimer’s Drug PBT2 Interacts with the Amyloid β 1–42 Peptide Differently than Other 8-Hydroxyquinoline Chelating Drugs
Alzheimer’s Drug PBT2 Interacts with the Amyloid β 1–42 Peptide Differently than Other 8-Hydroxyquinoline Chelating Drugs Open
Although Alzheimer's disease (AD) was first described over a century ago, it remains the leading cause of age-related dementia. Innumerable changes have been linked to the pathology of AD; however, there remains much discord regarding whic…
View article: Is Mini Beam Ready for Human Trials? Results of Randomized Study of Treating De-Novo Brain Tumors in Canines Using Linear Accelerator Generated Mini Beams
Is Mini Beam Ready for Human Trials? Results of Randomized Study of Treating De-Novo Brain Tumors in Canines Using Linear Accelerator Generated Mini Beams Open
The main challenge in treating malignant brain neoplasms lies in eradicating the tumor while minimizing treatment-related damage. Conventional radiation treatments are associated with considerable side effects. Synchrotron generated micro-…
View article: X-ray fluorescence microscopy methods for biological tissues
X-ray fluorescence microscopy methods for biological tissues Open
Synchrotron-based X-ray fluorescence microscopy is a flexible tool for identifying the distribution of trace elements in biological specimens across a broad range of sample sizes. The technique is not particularly limited by sample type an…
View article: Multimodal imaging of hemorrhagic transformation biomarkers in an ischemic stroke model.
Multimodal imaging of hemorrhagic transformation biomarkers in an ischemic stroke model. Open
Hemorrhagic transformation of ischemic stroke has devastating consequences, with high mortality and poor functional outcomes. Animal models of ischemic stroke also demonstrate the potential for hemorrhagic transformation, which complicates…
View article: The effects of trifluoperazine on brain edema, aquaporin-4 expression and metabolic markers during the acute phase of stroke using photothrombotic mouse model
The effects of trifluoperazine on brain edema, aquaporin-4 expression and metabolic markers during the acute phase of stroke using photothrombotic mouse model Open
Stroke is the second leading cause of death and the third leading cause of disability globally. Edema is a hallmark of stroke resulting from dysregulation of water homeostasis in the central nervous system (CNS) and plays the major role in…
View article: Iron Heterogeneity in Early Active Multiple Sclerosis Lesions
Iron Heterogeneity in Early Active Multiple Sclerosis Lesions Open
Objective Multiple sclerosis (MS) is a heterogeneous inflammatory demyelinating disease. Iron distribution is altered in MS patients' brains, suggesting iron liberation within active lesions amplifies demyelination and neurodegeneration. W…
View article: Solution Chemistry of Copper(II) Binding to Substituted 8-Hydroxyquinolines
Solution Chemistry of Copper(II) Binding to Substituted 8-Hydroxyquinolines Open
8-Hydroxyquinolines (8HQs) are a family of lipophilic metal ion chelators that have been used in a range of analytical and pharmaceutical applications over the last 100 years. More recently, CQ (clioquinol; 5-chloro-7-iodo-8-hydroxyquinoli…
View article: A Review of ex vivo Elemental Mapping Methods to Directly Image Changes in the Homeostasis of Diffusible Ions (Na+, K+, Mg2 +, Ca2 +, Cl–) Within Brain Tissue
A Review of ex vivo Elemental Mapping Methods to Directly Image Changes in the Homeostasis of Diffusible Ions (Na+, K+, Mg2 +, Ca2 +, Cl–) Within Brain Tissue Open
Diffusible ions (Na+, K+, Mg2+, Ca2+, Cl-) are vital for healthy function of all cells, especially brain cells. Unfortunately, the diffusible nature of these ions renders them difficult to study with traditional microscopy in situ within e…
View article: Sample preparation with sucrose cryoprotection dramatically alters Zn distribution in the rodent hippocampus, as revealed by elemental mapping
Sample preparation with sucrose cryoprotection dramatically alters Zn distribution in the rodent hippocampus, as revealed by elemental mapping Open
Transition metal ions (Fe, Mn, Cu, Zn) are essential for healthy brain function, but common sample preparations, such as sucrose cryo-protection alter their distribution, which can confound studies of brain disease.
View article: Using N-Terminal Coordination of Cu(II) and Ni(II) to Isolate the Coordination Environment of Cu(I) and Cu(II) Bound to His13 and His14 in Amyloid-β(4–16)
Using N-Terminal Coordination of Cu(II) and Ni(II) to Isolate the Coordination Environment of Cu(I) and Cu(II) Bound to His13 and His14 in Amyloid-β(4–16) Open
The amyloid-β (Aβ) peptide is a cleavage product of the amyloid precursor protein and has been implicated as a central player in Alzheimer's disease. The N-terminal end of Aβ is variable, and different proportions of these variable-length …
View article: A comparison of parametric and integrative approaches for X-ray fluorescence analysis applied to a Stroke model
A comparison of parametric and integrative approaches for X-ray fluorescence analysis applied to a Stroke model Open
Synchrotron X-ray fluorescence imaging enables visualization and quantification of microscopic distributions of elements. This versatile technique has matured to the point where it is used in a wide range of research fields. The method can…
View article: The N-terminal 14-mer model peptide of human Ctr1 can collect Cu(<scp>ii</scp>) from albumin. Implications for copper uptake by Ctr1
The N-terminal 14-mer model peptide of human Ctr1 can collect Cu(<span>ii</span>) from albumin. Implications for copper uptake by Ctr1 Open
The superior Cu(ii) affinity of human copper transporter 1 (hCtr1) drives copper acquisition from human serum albumin (HSA).
View article: The active site structure and catalytic mechanism of arsenite oxidase
The active site structure and catalytic mechanism of arsenite oxidase Open
Arsenite oxidase is thought to be an ancient enzyme, originating before the divergence of the Archaea and the Bacteria. We have investigated the nature of the molybdenum active site of the arsenite oxidase from the Alphaproteobacterium Rhi…
View article: Trifluoroselenomethionine: A New Unnatural Amino Acid
Trifluoroselenomethionine: A New Unnatural Amino Acid Open
Trifluoroselenomethionine (TFSeM), a new unnatural amino acid, was synthesized in seven steps from N ‐( tert ‐butoxycarbonyl)‐ l ‐aspartic acid tert ‐butyl ester. TFSeM shows enhanced methioninase‐induced cytotoxicity, relative to selenome…
View article: Mapping Alterations to the Endogenous Elemental Distribution within the Lateral Ventricles and Choroid Plexus in Brain Disorders Using X-Ray Fluorescence Imaging
Mapping Alterations to the Endogenous Elemental Distribution within the Lateral Ventricles and Choroid Plexus in Brain Disorders Using X-Ray Fluorescence Imaging Open
The choroid plexus and cerebral ventricles are critical structures for the production of cerebral spinal fluid (CSF) and play an important role in regulating ion and metal transport in the brain, however many aspects of its roles in normal…
View article: Three-dimensional labeling of newly formed bone using synchrotron radiation barium K-edge subtraction imaging
Three-dimensional labeling of newly formed bone using synchrotron radiation barium K-edge subtraction imaging Open
Bone is a dynamic tissue which exhibits complex patterns of growth as well as continuous internal turnover (i.e. remodeling). Tracking such changes can be challenging and thus a high resolution imaging-based tracer would provide a powerful…
View article: High Affinity Binding of Indium and Ruthenium Ions by Gastrins
High Affinity Binding of Indium and Ruthenium Ions by Gastrins Open
The peptide hormone gastrin binds two ferric ions with high affinity, and iron binding is essential for the biological activity of non-amidated forms of the hormone. Since gastrins act as growth factors in gastrointestinal cancers, and as …