M. John Albert
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View article: <i>Salmonella enterica</i> serotypes causing infection in Kuwait during 2018–2021, determined by multi-locus sequence typing or whole genome sequencing
<i>Salmonella enterica</i> serotypes causing infection in Kuwait during 2018–2021, determined by multi-locus sequence typing or whole genome sequencing Open
Salmonellosis due to non-typhoidal Salmonellae (NTS) is a zoonotic infection that has epidemiological uniqueness in different settings. The current study aimed to determine the serotypes and the genetic diversity of human Salmonella enteri…
View article: Whole-genome-based characterization of Escherichia albertii strains isolated from paediatric diarrhoeal cases in Kolkata, India
Whole-genome-based characterization of Escherichia albertii strains isolated from paediatric diarrhoeal cases in Kolkata, India Open
Escherichia albertii is a Gram-negative facultative anaerobic bacterium that causes diarrhoea in humans. This study shows the isolation of E. albertii from hospitalized paediatric diarrhoeal cases and genome-based characteristics with puta…
View article: Enteropathogenic Providencia alcalifaciens: A Subgroup of P. alcalifaciens That Causes Diarrhea
Enteropathogenic Providencia alcalifaciens: A Subgroup of P. alcalifaciens That Causes Diarrhea Open
Despite being considered a normal flora, Providencia alcalifaciens can cause diarrhea. In a previous study, strain 2939/90, obtained from a diarrheal patient, caused invasion and actin condensation in mammalian cells, and diarrhea in a rab…
View article: Enteropathogenic<i>Providencia alcalifaciens</i>: A subgroup of<i>P. alcalifaciens</i>that causes diarrhea
Enteropathogenic<i>Providencia alcalifaciens</i>: A subgroup of<i>P. alcalifaciens</i>that causes diarrhea Open
Even though Providencia alcalifaciens is considered as a normal flora of the large intestine, there are reports of it causing diarrhea. In a previous study, a strain, 2939/90 obtained as a pure stool culture from a dead diarrheal patient w…
View article: High Prevalence of Novel Sequence Types in Streptococcus pneumoniae That Caused Invasive Diseases in Kuwait in 2018
High Prevalence of Novel Sequence Types in Streptococcus pneumoniae That Caused Invasive Diseases in Kuwait in 2018 Open
Background: Multilocus sequence typing (MLST) is used to gain insight into the population genetics of bacteria in the form of sequence type (ST). MLST has been used to study the evolution and spread of virulent clones of Streptococcus pneu…
View article: Multidrug-Resistant and Extensively Drug-Resistant Escherichia coli in Sewage in Kuwait: Their Implications
Multidrug-Resistant and Extensively Drug-Resistant Escherichia coli in Sewage in Kuwait: Their Implications Open
In Kuwait, some sewage is discharged into the sea untreated, causing a health risk. Previously, we investigated the presence of pathogenic E. coli among the 140 isolates of E. coli cultured from the raw sewage from three sites in Kuwait. T…
View article: The whole-genome molecular epidemiology of sequential isolates of <i>Acinetobacter baumannii</i> colonizing the rectum of patients in an adult intensive care unit of a tertiary hospital
The whole-genome molecular epidemiology of sequential isolates of <i>Acinetobacter baumannii</i> colonizing the rectum of patients in an adult intensive care unit of a tertiary hospital Open
Acinetobacter baumannii is a nosocomial multidrug-resistant pathogen that specifically colonizes and infects patients in intensive care units (ICUs). Genome plasticity of A. baumannii may contribute to the rapid development of A. baumannii…
View article: Virulence and phylogenetic groups of Escherichia coli cultured from raw sewage in Kuwait
Virulence and phylogenetic groups of Escherichia coli cultured from raw sewage in Kuwait Open
In Kuwait, some untreated sewage is discharged into the sea which poses health risks. Therefore, we determined the virulence traits and the phylogenetic groups of E. coli cultured from raw sewage. Sewage was collected once every month for …
View article: Multiplex <b><i>gyrB</i></b> PCR Assay for Identification of <b><i>Acinetobacter baumannii</i></b> Is Validated by Whole Genome Sequence-Based Assays
Multiplex <b><i>gyrB</i></b> PCR Assay for Identification of <b><i>Acinetobacter baumannii</i></b> Is Validated by Whole Genome Sequence-Based Assays Open
Objective: A multiplex gyrB PCR assay has been used to diagnose Acinetobacter baumannii. However, this assay has not been validated against the gold standard DNA-DNA hybridization assay, which is a laborious method. DNA-DNA hybridization a…
View article: Epidemiology, Serogroups and Resistance of Salmonella During a 15-Year Period (2006–2020) in Kuwait
Epidemiology, Serogroups and Resistance of Salmonella During a 15-Year Period (2006–2020) in Kuwait Open
Continuous surveillance of Salmonella and its antimicrobial resistance is important for antibiotic policy formulation for invasive Salmonella infections.
View article: The 13-valent pneumococcal conjugate vaccine (PCV13) does not appear to provide much protection on combined invasive disease due to the six PCV13 non-PCV7 serotypes 1, 3, 5, 6A, 7F, and 19A in Kuwait during 2010–2019
The 13-valent pneumococcal conjugate vaccine (PCV13) does not appear to provide much protection on combined invasive disease due to the six PCV13 non-PCV7 serotypes 1, 3, 5, 6A, 7F, and 19A in Kuwait during 2010–2019 Open
Kuwait started immunizing children 65 y and all ages, compared to the pre-vaccination period, August 2003-July 2006 (period I). In the current study, we allowed additional time, August 2013-July 2019 (period III) for better vaccine effect …
View article: Additional file 1 of Diarrhea in an infant due to Shigella flexneri 1 carrying multiple cephalosporinase-encoding genes
Additional file 1 of Diarrhea in an infant due to Shigella flexneri 1 carrying multiple cephalosporinase-encoding genes Open
Additional file 1: Table S1. The 77 phylogroup 1 S. flexneri 1 isolates used for construction of phylogenetic tree in Fig. 2 are listed. These isolates were taken from Connor et al. [23].
View article: Genetic relatedness of serial rectal isolates of Acinetobacter baumannii in an adult intensive care unit of a tertiary hospital in Kuwait
Genetic relatedness of serial rectal isolates of Acinetobacter baumannii in an adult intensive care unit of a tertiary hospital in Kuwait Open
Acinetobacter baumannii is an opportunistic pathogen of intensive care unit (ICU) patients. A. baumannii colonizes many parts of the body including the gastrointestinal tract. Endemic and epidemic strains are polyclonal. There is no clarit…
View article: Gut microbiome of native Arab Kuwaitis
Gut microbiome of native Arab Kuwaitis Open
Background The human gut microbiome has an important role in health and disease. There is extensive geographical variation in the composition of the gut microbiome, however, little is known about the gut microbiome composition of people fr…
View article: Additional file 2 of Gut microbiome of native Arab Kuwaitis
Additional file 2 of Gut microbiome of native Arab Kuwaitis Open
Additional file 2: Table S1. The core gut microbiome is represented by 18 ASVs.
View article: Non-typhoidal Salmonella blood stream infection in Kuwait: Clinical and microbiological characteristics
Non-typhoidal Salmonella blood stream infection in Kuwait: Clinical and microbiological characteristics Open
Non-typhoidal Salmonella (NTS) bacteremia is a significant cause of morbidity and mortality worldwide. It is considered to be an emerging and neglected tropical disease in Africa. We studied this in two tertiary hospitals–Al Farwaniya and …
View article: Draft Genome Sequences of Two Strains of a Newly Described Species, Sphingobacterium cellulitidis
Draft Genome Sequences of Two Strains of a Newly Described Species, Sphingobacterium cellulitidis Open
The draft genome sequences of two strains of a newly described species, Sphingobacterium cellulitidis , have been determined. The type strain originated from cellulitis of a toe of a patient and the other strain from the environment. The s…
View article: MOESM1 of Carbuncle due to Salmonella Enteritidis: a novel presentation
MOESM1 of Carbuncle due to Salmonella Enteritidis: a novel presentation Open
Additional file 1: Table S1. Complete genome sequences to which strain CSE76F was compared.
View article: <i>Vibrio cholerae</i>O1 Imported from Iraq to Kuwait, 2015
<i>Vibrio cholerae</i>O1 Imported from Iraq to Kuwait, 2015 Open
To the Editor: The etiologic agent of the sixth pandemic of cholera was classical biotype of Vibrio cholerae O1. The ongoing seventh pandemic is caused by El Tor biotype. The biotypes are differentiated by phenotypic and genotypic characte…
View article: High Prevalence of New Delhi Metallo-β-Lactamase-1 (NDM-1) Producers among Carbapenem-Resistant Enterobacteriaceae in Kuwait
High Prevalence of New Delhi Metallo-β-Lactamase-1 (NDM-1) Producers among Carbapenem-Resistant Enterobacteriaceae in Kuwait Open
The aim of the study was to determine the prevalence of New Delhi metallo-β lactamase-1 (NDM-1) producing Enterobacteriaceae in Kuwait over a one year period. Consecutive Enterobacteriaceae isolates with reduced susceptibility to carbapene…
View article: Evaluation of the xTAG Gastrointestinal Pathogen Panel Assay for the Detection of Enteric Pathogens in Kuwait
Evaluation of the xTAG Gastrointestinal Pathogen Panel Assay for the Detection of Enteric Pathogens in Kuwait Open
Objective: To evaluate the utility of the Luminex xTAG gastrointestinal pathogen panel (GPP) assay in the detection of enteric pathogens from diarrheal stool samples in Kuwait. Materials and Methods: The Luminex xTAG GPP assay was used acc…