Marella de Bruijn
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View article: Resolving hematopoietic stem versus progenitor cell potential in the mouse dorsal aorta by differential <i>Runx1</i> +110 enhancer activity
Resolving hematopoietic stem versus progenitor cell potential in the mouse dorsal aorta by differential <i>Runx1</i> +110 enhancer activity Open
SUMMARY Hematopoietic stem cells (HSCs) are important in cell-based therapies for blood-related disorders. While progress has been made in the directed differentiation of pluripotent PSCs, such cultures promote hematopoietic progenitor cel…
View article: The emerging sequence grammar of 3D genome organisation
The emerging sequence grammar of 3D genome organisation Open
The multiplexed layers of regulatory processes and mechanisms within a cell are, to a degree, encoded in our genome. Unravelling the relationship between DNA sequence and molecular processes is crucial for understanding evolution, interpre…
View article: The Emerging Sequence Grammar of 3D Genome Organisation
The Emerging Sequence Grammar of 3D Genome Organisation Open
The multiplexed layers of regulatory processes and mechanisms within a cell are, to a degree, encoded in our genome. Unravelling the relationship between DNA sequence and molecular processes is crucial for understanding evolution, interpre…
View article: Spatiotemporal dynamics of fetal liver hematopoietic niches
Spatiotemporal dynamics of fetal liver hematopoietic niches Open
Embryonic hematopoietic cells develop in the fetal liver (FL), surrounded by diverse non-hematopoietic stromal cells. However, the spatial organization and cytokine production patterns of the stroma during FL development remain poorly unde…
View article: Hemogenic endothelium of the vitelline and umbilical arteries is the major contributor to mouse fetal lympho-myelopoiesis
Hemogenic endothelium of the vitelline and umbilical arteries is the major contributor to mouse fetal lympho-myelopoiesis Open
Summary Embryonic hematopoiesis consists of distinct waves originating in rapid succession from different anatomical locations. Hematopoietic progenitors appearing earlier than definitive hematopoietic stem cells (HSCs) play key roles in f…
View article: Tracking early mammalian organogenesis – prediction and validation of differentiation trajectories at whole organism scale
Tracking early mammalian organogenesis – prediction and validation of differentiation trajectories at whole organism scale Open
Early organogenesis represents a key step in animal development, during which pluripotent cells diversify to initiate organ formation. Here, we sampled 300,000 single-cell transcriptomes from mouse embryos between E8.5 and E9.5 in 6-h inte…
View article: Spatiotemporal dynamics of cytokines expression dictate fetal liver hematopoiesis
Spatiotemporal dynamics of cytokines expression dictate fetal liver hematopoiesis Open
During embryogenesis, yolk-sac and intra-embryonic-derived hematopoietic progenitors, comprising the precursors of adult hematopoietic stem cells, converge into the fetal liver. With a new staining strategy, we defined all non-hematopoieti…
View article: Yolk sac cell atlas reveals multiorgan functions during human early development
Yolk sac cell atlas reveals multiorgan functions during human early development Open
The extraembryonic yolk sac (YS) ensures delivery of nutritional support and oxygen to the developing embryo but remains ill-defined in humans. We therefore assembled a comprehensive multiomic reference of the human YS from 3 to 8 postconc…
View article: P1326: HEMATOPOIETIC STEM/PROGENITOR CELLS ORIGINATING FROM EXTRAEMBRYONIC ARTERIAL VESSELS ARE THE MAJOR CONTRIBUTORS TO MOUSE FETAL LYMPHO-MYELOPOIESIS
P1326: HEMATOPOIETIC STEM/PROGENITOR CELLS ORIGINATING FROM EXTRAEMBRYONIC ARTERIAL VESSELS ARE THE MAJOR CONTRIBUTORS TO MOUSE FETAL LYMPHO-MYELOPOIESIS Open
Topic: 23. Hematopoiesis, stem cells and microenvironment Background: In mammals, embryonic hematopoiesis takes place in discrete but overlapping waves. Hematopoietic Stem Cells (HSC) generation is preceded by the appearance of HSC-indepen…
View article: Tracking Early Mammalian Organogenesis – Prediction and Validation of Differentiation Trajectories at Whole Organism Scale
Tracking Early Mammalian Organogenesis – Prediction and Validation of Differentiation Trajectories at Whole Organism Scale Open
Early organogenesis represents a key step in animal development, where pluripotent cells divide and diversify to initiate formation of all major organs. Here, we used scRNA-Seq to profile over 300,000 single cell transcriptomes sampled in …
View article: A gene regulatory network of the endothelial-to-hematopoietic transition
A gene regulatory network of the endothelial-to-hematopoietic transition Open
These datasets are from the generation, and subsequent analysis, of a gene regulatory network (GRN) model of the endothelial-to-hematopoietic (EHT) process. This GRN model was created through integrating RNA-seq and ATAC-seq datasets, in c…
View article: Multi-organ functions of yolk sac during human early development
Multi-organ functions of yolk sac during human early development Open
The yolk sac (YS) represents an evolutionarily-conserved extraembryonic structure that ensures timely delivery of nutritional support and oxygen to the developing embryo. However, the YS remains ill-defined in humans. We therefore assemble…
View article: A KMT2A-AFF1 gene regulatory network highlights the role of core transcription factors and reveals the regulatory logic of key downstream target genes
A KMT2A-AFF1 gene regulatory network highlights the role of core transcription factors and reveals the regulatory logic of key downstream target genes Open
Regulatory interactions mediated by transcription factors (TFs) make up complex networks that control cellular behavior. Fully understanding these gene regulatory networks (GRNs) offers greater insight into the consequences of disease-caus…
View article: Dynamic<i>Runx1</i>chromatin boundaries affect gene expression in hematopoietic development
Dynamic<i>Runx1</i>chromatin boundaries affect gene expression in hematopoietic development Open
The transcription factor RUNX1 is a critical regulator of developmental hematopoiesis and is frequently disrupted in leukemia. Runx1 is a large, complex gene that is expressed from two alternative promoters under the spatiotemporal control…
View article: Phenotypic analysis of an MLL-AF4 gene regulatory network reveals indirect CASP9 repression as a mode of inducing apoptosis resistance
Phenotypic analysis of an MLL-AF4 gene regulatory network reveals indirect CASP9 repression as a mode of inducing apoptosis resistance Open
Regulatory interactions mediated by transcription factors (TFs) make up complex networks that control cellular behavior. Fully understanding these gene regulatory networks (GRNs) offers greater insight into the consequences of disease-caus…
View article: Gata3 targets Runx1 in the embryonic haematopoietic stem cell niche
Gata3 targets Runx1 in the embryonic haematopoietic stem cell niche Open
Runx1 is an important haematopoietic transcription factor as stressed by its involvement in a number of haematological malignancies. Furthermore, it is a key regulator of the emergence of the first haematopoietic stem cells (HSCs) during d…
View article: Microhomologies are prevalent at Cas9-induced larger deletions
Microhomologies are prevalent at Cas9-induced larger deletions Open
The CRISPR system is widely used in genome editing for biomedical research. Here, using either dual paired Cas9D10A nickases or paired Cas9 nuclease we characterize unintended larger deletions at on-target sites that frequently evade commo…
View article: Cell-extrinsic hematopoietic impact of Ezh2 inactivation in fetal liver endothelial cells
Cell-extrinsic hematopoietic impact of Ezh2 inactivation in fetal liver endothelial cells Open
Key Points Ezh2 is dispensable for fetal HSCs. Inactivation of Ezh2 in FL endothelium leads to cell-extrinsically mediated anemia.