Roland Malli
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View article: Targeting hexokinase 2 to induce breast cancer cell senescence
Targeting hexokinase 2 to induce breast cancer cell senescence Open
Background and Purpose Hexokinase 2 (HK2) is a key enzyme linked to high tumour cell proliferation. Its inhibitors such as 3‐bromopyruvic acid (3‐BP) induce cancer cell death, highlighting HK2 modulation as potential anti‐cancer treatment.…
View article: A quad‐cistronic fluorescent biosensor system for real‐time detection of subcellular Ca<sup>2+</sup> signals
A quad‐cistronic fluorescent biosensor system for real‐time detection of subcellular Ca<sup>2+</sup> signals Open
Background and Purpose The calcium ion (Ca 2+ ) is a versatile cellular messenger regulating a variety of biological processes. Compounds modulating subcellular Ca 2+ signals hold substantial pharmacological potential. Advances in fluoresc…
View article: Presenilin-1 controls glucose metabolism and identity of pancreatic beta cells
Presenilin-1 controls glucose metabolism and identity of pancreatic beta cells Open
Presenilin 1 is an endoplasmic reticulum protein, most known for its role in pathogenesis of familial Alzheimer’s Disease (AD). Presenilin 1 has been attributed roles in intracellular calcium homeostasis in the brain, as well as in the pan…
View article: Tracking Intracellular Labile Iron with a Genetically Encoded Fluorescent Reporter System Based on Protein Stability
Tracking Intracellular Labile Iron with a Genetically Encoded Fluorescent Reporter System Based on Protein Stability Open
Here, we developed IronFist, a genetically encoded fluorescent reporter that enables dynamic tracking of labile ferrous ions (Fe2+) in live cells. IronFist is a bicistronic system combining the iron-sensitive hemerythrin-like (Hr) domain f…
View article: Photopharmacology reveals high-specificity linkage of Ca2+ entry at TRPC6 nanodomains to NFAT activation in mast cells
Photopharmacology reveals high-specificity linkage of Ca2+ entry at TRPC6 nanodomains to NFAT activation in mast cells Open
Introduction Photopharmacology has recently emerged as a strategy for high-precision modulation of immune functions. Here we explored efficiency and specificity of interventions based on light-induced TRPC6 activation in the RBL-2H3 mast c…
View article: Annexin A5 controls VDAC1-dependent mitochondrial Ca2+ homeostasis and determines cellular susceptibility to apoptosis
Annexin A5 controls VDAC1-dependent mitochondrial Ca2+ homeostasis and determines cellular susceptibility to apoptosis Open
Annexin A5 (AnxA5) is a Ca 2+ -dependent phospholipid-binding protein associated with the regulation of intracellular Ca 2+ homeostasis. However, the precise role of AnxA5 in controlling mitochondrial Ca 2+ signaling remains elusive. Here,…
View article: Development of a Genetically Encoded Live-Cell Iron Mapping System
Development of a Genetically Encoded Live-Cell Iron Mapping System Open
Any disturbances in cellular iron regulation can lead to dysfunctions and various diseases. However, our current understanding of iron dynamics in living cells is hampered by the lack of high-resolution iron reporter systems capable of mon…
View article: Acute suppression of mitochondrial ATP production prevents apoptosis and provides an essential signal for NLRP3 inflammasome activation
Acute suppression of mitochondrial ATP production prevents apoptosis and provides an essential signal for NLRP3 inflammasome activation Open
How mitochondria reconcile roles in functionally divergent cell death pathways of apoptosis and NLRP3 inflammasome-mediated pyroptosis remains elusive, as is their precise role in NLRP3 activation and the evolutionarily conserved physiolog…
View article: Visualizing VDAC1 in live cells using a tetracysteine tag
Visualizing VDAC1 in live cells using a tetracysteine tag Open
The voltage-dependent anion channel 1 (VDAC1) is a crucial gatekeeper in the outer mitochondrial membrane, controlling metabolic and energy homeostasis. The available methodological approaches fell short of accurate visualization of VDAC1 …
View article: Development of a Dual Reporter System to Simultaneously Visualize Ca<sup>2+</sup> Signals and AMPK Activity
Development of a Dual Reporter System to Simultaneously Visualize Ca<sup>2+</sup> Signals and AMPK Activity Open
In this study, we introduce a new separation of phases-based activity reporter of kinase (SPARK) for AMP-activated kinase (AMPK), named AMPK-SPARK, which reports the AMPK activation by forming bright fluorescent clusters. Furthermore, we i…
View article: Slack K+ channels confer protection against myocardial ischaemia/reperfusion injury
Slack K+ channels confer protection against myocardial ischaemia/reperfusion injury Open
Aims Na+-activated Slack potassium (K+) channels are increasingly recognized as regulators of neuronal activity, yet little is known about their role in the cardiovascular system. Slack activity increases when intracellular Na+ concentrati…
View article: Hexokinase 1 forms rings that regulate mitochondrial fission during energy stress
Hexokinase 1 forms rings that regulate mitochondrial fission during energy stress Open
Metabolic enzymes can adapt during energy stress, but the consequences of these adaptations remain understudied. Here, we discovered that hexokinase 1 (HK1), a key glycolytic enzyme, forms rings around mitochondria during energy stress. Th…
View article: Probing intracellular potassium dynamics in neurons with the genetically encoded sensor lc-LysM GEPII 1.0 in vitro and in vivo
Probing intracellular potassium dynamics in neurons with the genetically encoded sensor lc-LysM GEPII 1.0 in vitro and in vivo Open
Neuronal activity is accompanied by a net outflow of potassium ions (K + ) from the intra- to the extracellular space. While extracellular [K + ] changes during neuronal activity are well characterized, intracellular dynamics have been les…
View article: mitoBKCa is functionally expressed in murine and human breast cancer cells and potentially contributes to metabolic reprogramming
mitoBKCa is functionally expressed in murine and human breast cancer cells and potentially contributes to metabolic reprogramming Open
Alterations in the function of K + channels such as the voltage- and Ca 2+ -activated K + channel of large conductance (BK Ca ) reportedly promote breast cancer (BC) development and progression. Underlying molecular mechanisms remain, howe…
View article: Author Response: mitoBKCa is functionally expressed in murine and human breast cancer cells and potentially contributes to metabolic reprogramming
Author Response: mitoBKCa is functionally expressed in murine and human breast cancer cells and potentially contributes to metabolic reprogramming Open
Alterations in the function of K+ channels such as the voltage- and Ca2+ activated K+ channel of large conductance (BKCa) reportedly promote breast cancer (BC) development and progression. Underlying molecular mechanisms remain, however, e…
View article: Reviewer #1 (Public Review): mitoBKCa is functionally expressed in murine and human breast cancer cells and potentially contributes to metabolic reprogramming
Reviewer #1 (Public Review): mitoBKCa is functionally expressed in murine and human breast cancer cells and potentially contributes to metabolic reprogramming Open
Alterations in the function of K+ channels such as the voltage- and Ca2+ activated K+ channel of large conductance (BKCa) reportedly promote breast cancer (BC) development and progression. Underlying molecular mechanisms remain, however, e…
View article: mitoBKCa is functionally expressed in murine and human breast cancer cells and potentially contributes to metabolic reprogramming
mitoBKCa is functionally expressed in murine and human breast cancer cells and potentially contributes to metabolic reprogramming Open
Summary Alterations in the function of K+ channels such as the voltage- and Ca2+ activated K+ channel of large conductance (BKCa) reportedly promote breast cancer (BC) development and progression. Underlying molecular mechanisms remain, ho…
View article: Reviewer #3 (Public Review): mitoBKCa is functionally expressed in murine and human breast cancer cells and potentially contributes to metabolic reprogramming
Reviewer #3 (Public Review): mitoBKCa is functionally expressed in murine and human breast cancer cells and potentially contributes to metabolic reprogramming Open
Alterations in the function of K+ channels such as the voltage- and Ca2+ activated K+ channel of large conductance (BKCa) reportedly promote breast cancer (BC) development and progression. Underlying molecular mechanisms remain, however, e…
View article: Genetically encoded fluorescent sensor to monitor intracellular arginine methylation
Genetically encoded fluorescent sensor to monitor intracellular arginine methylation Open
Arginine methylation (ArgMet), as a post-translational modification, plays crucial roles in RNA processing, transcriptional regulation, signal transduction, DNA repair, apoptosis and liquid-liquid phase separation (LLPS). Since arginine me…
View article: Probing Intracellular Potassium Dynamics in Neurons with the Genetically Encoded Sensor lc-LysM GEPII 1.0 in vitro and in vivo
Probing Intracellular Potassium Dynamics in Neurons with the Genetically Encoded Sensor lc-LysM GEPII 1.0 in vitro and in vivo Open
Neuronal activity is accompanied by a net outflow of potassium ions (K+) from the intra- to the extracellular space. While extracellular [K+] changes during neuronal activity are well characterized, intracellular dynamics have been less we…
View article: mitoBKCa is functionally expressed in murine and human breast cancer cells and potentially contributes to metabolic reprogramming
mitoBKCa is functionally expressed in murine and human breast cancer cells and potentially contributes to metabolic reprogramming Open
Alterations in the function of K + channels such as the voltage- and Ca 2+ -activated K + channel of large conductance (BK Ca ) reportedly promote breast cancer (BC) development and progression. Underlying molecular mechanisms remain, howe…
View article: mitoBKCa is functionally expressed in murine and human breast cancer cells and promotes metabolic reprogramming
mitoBKCa is functionally expressed in murine and human breast cancer cells and promotes metabolic reprogramming Open
Summary Alterations in the function of K+ channels such as the voltage- and Ca2+ activated K+ channel of large conductance (BKCa) reportedly promote breast cancer (BC) development and progression. Underlying molecular mechanisms remain, ho…
View article: mitoBK<sub>Ca</sub>is functionally expressed in murine and human breast cancer cells and potentially contributes to metabolic reprogramming
mitoBK<sub>Ca</sub>is functionally expressed in murine and human breast cancer cells and potentially contributes to metabolic reprogramming Open
Summary Alterations in the function of K + channels such as the voltage- and Ca 2+ activated K + channel of large conductance (BK Ca ) reportedly promote breast cancer (BC) development and progression. Underlying molecular mechanisms remai…
View article: Annexin-A5 is fundamental for VDAC1-dependent mitochondrial Ca2+ homeostasis and determines the susceptibility to apoptosis.
Annexin-A5 is fundamental for VDAC1-dependent mitochondrial Ca2+ homeostasis and determines the susceptibility to apoptosis. Open
Annexin-A5 (AnxA5) is a Ca2+-dependent phospholipid-binding protein associated with the regulation of intracellular Ca2+ homeostasis. However, the precise role of AnxA5 in controlling mitochondrial Ca2+ signaling remained elusive. Here, we…
View article: Visualizing interactions of VDAC1 in live cells using a tetracysteine tag
Visualizing interactions of VDAC1 in live cells using a tetracysteine tag Open
Summary The voltage-dependent anion channel 1 (VDAC1) is a crucial gatekeeper in the outer mitochondrial membrane, controlling metabolic and energy homeostasis. The available methodological approaches fell short of accurate visualization o…
View article: Analysis of a non‐lethal biallelic frameshift mutation in <scp><i>ZMPSTE24</i></scp> reveals utilization of alternative translation initiation codons
Analysis of a non‐lethal biallelic frameshift mutation in <span><i>ZMPSTE24</i></span> reveals utilization of alternative translation initiation codons Open
Restrictive dermopathy (RD) is a lethal condition caused by biallelic loss‐of‐function mutations in ZMPSTE24 , whereas mutations preserving residual enzymatic activity of the ZMPSTE24 protein lead to the milder mandibuloacral dysplasia wit…