Cuiqing Ma
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View article: A Genetically Encoded Biosensor for Characterizing Transport and Metabolism of Glutarate
A Genetically Encoded Biosensor for Characterizing Transport and Metabolism of Glutarate Open
Glutarate is a platform chemical with diversified applications. It is also an endogenous metabolite involved in various physiological processes. Deficiency in glutaryl‐CoA dehydrogenase (GcdH) for glutarate catabolism induces the inherited…
View article: A genetically encoded biosensor for point-of-care and live-cell detection of d-2-hydroxyglutarate
A genetically encoded biosensor for point-of-care and live-cell detection of d-2-hydroxyglutarate Open
D-2-Hydroxyglutarate (D-2-HG) is a functional endogenous metabolite in various domains of life. Its abnormal accumulation promotes human tumorigenesis. Convenient D-2-HG testing for diagnosis and prognosis of D-2-HG-related diseases remain…
View article: Production of dicarboxylates from ω-amino acids using a cofactor- and co-substrate-free in vitro biosynthetic system
Production of dicarboxylates from ω-amino acids using a cofactor- and co-substrate-free in vitro biosynthetic system Open
Dicarboxylates are valuable platform compounds with a broad range of applications. The in vitro biosynthetic system used to produce dicarboxylates from ω-amino acids via the natural pathway requires costly cofactors and co-substrates, whic…
View article: On Metric Choice in Dimension Reduction for Fréchet Regression
On Metric Choice in Dimension Reduction for Fréchet Regression Open
Summary Fréchet regression is becoming a mainstay in modern data analysis for analysing non‐traditional data types belonging to general metric spaces. This novel regression method is especially useful in the analysis of complex health data…
View article: Acetate production from corn stover hydrolysate using recombinant Escherichia coli BL21 (DE3) with an EP-bifido pathway
Acetate production from corn stover hydrolysate using recombinant Escherichia coli BL21 (DE3) with an EP-bifido pathway Open
The results presented here provide a promising alternative for acetate production with low cost substrate. Besides acetate production, other biotechnological processes might also be developed for other acetyl-CoA derivatives production wit…
View article: On metric choice in dimension reduction for Fréchet regression
On metric choice in dimension reduction for Fréchet regression Open
Fréchet regression is becoming a mainstay in modern data analysis for analyzing non-traditional data types belonging to general metric spaces. This novel regression method is especially useful in the analysis of complex health data such as…
View article: An Ultrasensitive Biosensor for Probing Subcellular Distribution and Mitochondrial Transport of <scp>l</scp>‐2‐Hydroxyglutarate
An Ultrasensitive Biosensor for Probing Subcellular Distribution and Mitochondrial Transport of <span>l</span>‐2‐Hydroxyglutarate Open
l ‐2‐Hydroxyglutarate ( l ‐2‐HG) is a functionally compartmentalized metabolite involved in various physiological processes. However, its subcellular distribution and mitochondrial transport remain unclear owing to technical limitations. I…
View article: Metabolic functions of misunderstood D-2-hydroxyglutarate
Metabolic functions of misunderstood D-2-hydroxyglutarate Open
Isocitrate dehydrogenase mutation-induced D-2-hydroxyglutarate (D-2-HG) accumulation may promote the tumorigenesis of numerous cancers. D-2-HG produced by several promiscuous enzymatic reactions under normal conditions is also toxic to org…
View article: Poly-3-hydroxybutyrate production from acetate by recombinant Pseudomonas stutzeri with blocked L-leucine catabolism and enhanced growth in acetate
Poly-3-hydroxybutyrate production from acetate by recombinant Pseudomonas stutzeri with blocked L-leucine catabolism and enhanced growth in acetate Open
Acetate is a low-cost feedstock for the production of different bio-chemicals. Electrochemical reduction of CO 2 into acetate and subsequent acetate fermentation is a promising method for transforming CO 2 into value-added chemicals. Howev…
View article: Quantitative Risk Assessment of Product Disulfide Bond Reduction in A Recombinant Protein Manufacturing
Quantitative Risk Assessment of Product Disulfide Bond Reduction in A Recombinant Protein Manufacturing Open
Disulfide bond reduction occurred now and then during a recombinant protein manufacturing. Their reduction often led in the loss of batches. Various prevention measures were developed. Their implementation in a manufacturing inevitably inc…
View article: A Selective Fluorescent l-Lactate Biosensor Based on an l-Lactate-Specific Transcription Regulator and Förster Resonance Energy Transfer
A Selective Fluorescent l-Lactate Biosensor Based on an l-Lactate-Specific Transcription Regulator and Förster Resonance Energy Transfer Open
Selective detection of l-lactate levels in foods, clinical, and bacterial fermentation samples has drawn intensive attention. Many fluorescent biosensors based on non-stereoselective recognition elements have been developed for lactate det…
View article: Metabolic Mechanism and Physiological Role of Glycerol 3-Phosphate in Pseudomonas aeruginosa PAO1
Metabolic Mechanism and Physiological Role of Glycerol 3-Phosphate in Pseudomonas aeruginosa PAO1 Open
In view of the intrinsic resistance of Pseudomonas aeruginosa to antibiotics and its potential to acquire resistance to current antibiotics, there is an urgent need to develop novel therapeutic options for the treatment of infections cause…
View article: Non-Sterilized Fermentation of 2,3-Butanediol with Seawater by Metabolic Engineered Fast-Growing Vibrio natriegens
Non-Sterilized Fermentation of 2,3-Butanediol with Seawater by Metabolic Engineered Fast-Growing Vibrio natriegens Open
Sustainable and environment-friendly microbial fermentation processes have been developed to produce numerous chemicals. However, the high energy input required for sterilization and substantial fresh water consumption restrict the economi…
View article: Enhanced In Vitro Cascade Catalysis of Glycerol into Pyruvate and Acetoin by Integration with Dihydroxy Acid Dehydratase from Paralcaligenes ureilyticus
Enhanced In Vitro Cascade Catalysis of Glycerol into Pyruvate and Acetoin by Integration with Dihydroxy Acid Dehydratase from Paralcaligenes ureilyticus Open
Recently, an in vitro enzymatic cascade was constructed to transform glycerol into the high-value platform chemical pyruvate. However, the low activity of dihydroxy acid dehydratase from Sulfolobus solfataricus (SsDHAD) limited the efficie…
View article: Dehydrogenation Mechanism of Three Stereoisomers of Butane-2,3-Diol in Pseudomonas putida KT2440
Dehydrogenation Mechanism of Three Stereoisomers of Butane-2,3-Diol in Pseudomonas putida KT2440 Open
Pseudomonas putida KT2440 is a promising chassis of industrial biotechnology due to its metabolic versatility. Butane-2,3-diol (2,3-BDO) is a precursor of numerous value-added chemicals. It is also a microbial metabolite which widely exist…
View article: An l-2-hydroxyglutarate biosensor based on specific transcriptional regulator LhgR
An l-2-hydroxyglutarate biosensor based on specific transcriptional regulator LhgR Open
l -2-Hydroxyglutarate ( l- 2-HG) plays important roles in diverse physiological processes, such as carbon starvation response, tumorigenesis, and hypoxic adaptation. Despite its importance and intensively studied metabolism, regulation of …
View article: Production of Ethylene Glycol from Glycerol Using an In Vitro Enzymatic Cascade
Production of Ethylene Glycol from Glycerol Using an In Vitro Enzymatic Cascade Open
Glycerol is a readily available and inexpensive substance that is mostly generated during biofuel production processes. In order to ensure the viability of the biofuel industry, it is essential to develop complementing technologies for the…
View article: Kinetic characteristics of long‐term repeated fed‐batch (LtRFb) <scp>l‐</scp>lactic acid fermentation by a <i>Bacillus coagulans</i> strain
Kinetic characteristics of long‐term repeated fed‐batch (LtRFb) <span>l‐</span>lactic acid fermentation by a <i>Bacillus coagulans</i> strain Open
Application of degradable plastics is the most critical solution to plastic pollution. As the precursor of biodegradable plastic PLA (polylactic acid), efficient production of l‐ lactic acid is vital for the commercial replacement of tradi…
View article: Efficient 2,3-butanediol production from whey powder using metabolically engineered Klebsiella oxytoca
Efficient 2,3-butanediol production from whey powder using metabolically engineered Klebsiella oxytoca Open
Background: Whey is a major pollutant generated by the dairy industry. To decrease environmental pollution caused by the industrial release of whey, new prospects for its utilization need to be urgently explored. Here, we investigated the …
View article: A fluorescent l-2-hydroxyglutarate biosensor
A fluorescent l-2-hydroxyglutarate biosensor Open
l -2-Hydroxyglutarate ( l -2-HG) plays important roles in diverse physiological processes, such as carbon starvation response, tumorigenesis, and hypoxic adaptation. Despite its importance and intensively studied metabolism, regulation of …
View article: Efficient Production of 2,3-Butanediol from whey Powder by Metabolic Engineered Klebsiella Oxytoca
Efficient Production of 2,3-Butanediol from whey Powder by Metabolic Engineered Klebsiella Oxytoca Open
Backgrounds: Whey is the major pollution source from the dairy industry. Exploring new outlets for whey utilization is urgently needed to decline its environmental pollution. In this study, we explored the possibility of using whey powder …
View article: Metabolic Engineering of Bacillus licheniformis for Production of Acetoin
Metabolic Engineering of Bacillus licheniformis for Production of Acetoin Open
Acetoin is a potential platform compound for a variety of chemicals. Bacillus licheniformis MW3, a thermophilic and generally regarded as safe (GRAS) microorganism, can produce 2,3-butanediol with a high concentration, yield, and productiv…