Magdalena M. Magaj
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View article: Mechanisms of cilia regeneration in Xenopus multiciliated epithelium in vivo
Mechanisms of cilia regeneration in Xenopus multiciliated epithelium in vivo Open
Cilia regeneration is a physiological event, and while studied extensively in unicellular organisms, it remains poorly understood in vertebrates. In this study, using Xenopus multiciliated cells (MCCs), we demonstrate that, unlike unicellu…
View article: Accurate and fast segmentation of filaments and membranes in micrographs and tomograms with TARDIS
Accurate and fast segmentation of filaments and membranes in micrographs and tomograms with TARDIS Open
Segmentation of macromolecular structures is the primary bottleneck for studying biomolecules and their organization with electron microscopy in 2D/3D – requiring months of manual effort. Transformer-based Rapid Dimensionless Instance Segm…
View article: Using 3D Large Scale Tomography to Study Force Generation in the Mitotic Spindle
Using 3D Large Scale Tomography to Study Force Generation in the Mitotic Spindle Open
View article: Collapse of late endosomal pH elicits a rapid Rab7 response via the V-ATPase and RILP
Collapse of late endosomal pH elicits a rapid Rab7 response via the V-ATPase and RILP Open
Endosomal-lysosomal trafficking is accompanied by the acidification of endosomal compartments by the H+-V-ATPase to reach low lysosomal pH. Disruption of the correct pH impairs lysosomal function and the balance of protein synthesis and de…
View article: iNOS is necessary for GBP-mediated T. gondii clearance in murine macrophages via vacuole nitration and intravacuolar network collapse
iNOS is necessary for GBP-mediated T. gondii clearance in murine macrophages via vacuole nitration and intravacuolar network collapse Open
Toxoplasma gondii is an obligate intracellular parasite of rodents and humans. Interferon-inducible guanylate binding proteins (GBPs) are mediators of T. gondii clearance, however, this mechanism is incomplete. Here, using automated spatia…
View article: Chromokinesin Klp-19 regulates microtubule overlap and dynamics during anaphase in <i>C. elegans</i>
Chromokinesin Klp-19 regulates microtubule overlap and dynamics during anaphase in <i>C. elegans</i> Open
Recent studies have highlighted the significance of the spindle midzone, the region between the segregating chromosomes, in ensuring proper chromosome segregation. By combining 3D electron tomography, cutting-edge light microscopy and a no…
View article: Mechanisms of cilia regeneration in <i>Xenopus</i> multiciliated epithelium <i>in vivo</i>
Mechanisms of cilia regeneration in <i>Xenopus</i> multiciliated epithelium <i>in vivo</i> Open
SUMMARY Cilia regeneration is a physiological event, and while studied extensively in unicellular organisms, it remains poorly understood in vertebrates. In this study, using Xenopus multiciliated cells (MCCs) as a model, we demonstrate th…
View article: Mechanisms of Cilia Regeneration in <i>Xenopus</i> Multiciliated Epithelium <i>in vivo</i>
Mechanisms of Cilia Regeneration in <i>Xenopus</i> Multiciliated Epithelium <i>in vivo</i> Open
View article: Ndc1 drives nuclear pore complex assembly independent of membrane biogenesis to promote nuclear formation and growth
Ndc1 drives nuclear pore complex assembly independent of membrane biogenesis to promote nuclear formation and growth Open
The nuclear envelope (NE) assembles and grows from bilayer lipids produced at the endoplasmic reticulum (ER). How ER membrane incorporation coordinates with assembly of nuclear pore complexes (NPCs) to generate a functional NE is not well …
View article: Author response: Ndc1 drives nuclear pore complex assembly independent of membrane biogenesis to promote nuclear formation and growth
Author response: Ndc1 drives nuclear pore complex assembly independent of membrane biogenesis to promote nuclear formation and growth Open
View article: Impact of Ionic Liquids on Induction of Wheat Microspore Embryogenesis and Plant Regeneration
Impact of Ionic Liquids on Induction of Wheat Microspore Embryogenesis and Plant Regeneration Open
Ionic liquids are novel compounds with unique chemical and physical properties. They can be received based on synthetic auxins like 2,4-dichlorophenoxyacetic acid or dicamba, which are commonly used hormones in microspore embryogenesis. Ne…