Makoto Itakura
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View article: Medermycin Inhibits TNFα-Promoted Inflammatory Reaction in Human Synovial Fibroblasts
Medermycin Inhibits TNFα-Promoted Inflammatory Reaction in Human Synovial Fibroblasts Open
Synovial inflammation plays a crucial role in the destruction of joints and the experience of pain in osteoarthritis (OA). Emerging evidence suggests that certain antibiotic agents and their derivatives possess anti-inflammatory properties…
View article: Jietacin Derivative Inhibits TNF-α-Mediated Inflammatory Cytokines Production via Suppression of the NF-κB Pathway in Synovial Cells
Jietacin Derivative Inhibits TNF-α-Mediated Inflammatory Cytokines Production via Suppression of the NF-κB Pathway in Synovial Cells Open
Synovial inflammation plays a central role in joint destruction and pain in osteoarthritis (OA). The NF-κB pathway plays an important role in the inflammatory process and is activated in OA. A previous study reported that a jietacin deriva…
View article: COL1A1 and COL3A1 elevation and regulation by β2-microglobulin in subsynovial connective tissues of patients undergoing hemodialysis with carpal tunnel syndrome
COL1A1 and COL3A1 elevation and regulation by β2-microglobulin in subsynovial connective tissues of patients undergoing hemodialysis with carpal tunnel syndrome Open
Purpose: Carpal tunnel syndrome (CTS) constitutes a typical complication of patients receiving long-term hemodialysis (HD). However, the mechanisms underlying the manifestation of CTS in patients receiving long-term hemodialysis remain to …
View article: TNF-α Suppresses Apelin Receptor Expression in Mouse Quadriceps Femoris-Derived Cells
TNF-α Suppresses Apelin Receptor Expression in Mouse Quadriceps Femoris-Derived Cells Open
Expression of the apelin receptor, APJ, in skeletal muscle (SM) is known to decrease with age, but the underlying mechanism remains unclear. Increased tumor necrosis factor (TNF)-α levels are observed in SM with age and are associated with…
View article: ANGT_HUMAN[448–462], an Anorexigenic Peptide Identified Using Plasma Peptidomics
ANGT_HUMAN[448–462], an Anorexigenic Peptide Identified Using Plasma Peptidomics Open
The discovery of bioactive peptides is an important research target that enables the elucidation of the pathophysiology of human diseases and provides seeds for drug discovery. Using a large number of native peptides previously identified …
View article: Origin of M2 Mϕ and its macrophage polarization by TGF-β in a mice intervertebral injury model
Origin of M2 Mϕ and its macrophage polarization by TGF-β in a mice intervertebral injury model Open
Introduction Studies have identified the presence of M1 and M2 macrophages (Mϕ) in injured intervertebral discs (IVDs). However, the origin and polarization-regulatory factor of M2 Mϕ are not fully understood. TGF-β is a regulatory factor …
View article: D1 Receptor Mediated Dopaminergic Neurotransmission Facilitates Remote Memory of Contextual Fear Conditioning
D1 Receptor Mediated Dopaminergic Neurotransmission Facilitates Remote Memory of Contextual Fear Conditioning Open
Dopaminergic neurotransmission via dopamine D1 receptors (D1Rs) is considered to play an important role not only in reward-based learning but also in aversive learning. The contextual and auditory cued fear conditioning tests involve the p…
View article: Importance of the Q/N-rich Segment for Protein Stability and Activity of Endogenous Mouse TDP-43
Importance of the Q/N-rich Segment for Protein Stability and Activity of Endogenous Mouse TDP-43 Open
TAR DNA-binding protein 43 kDa (TDP-43), a nuclear protein, plays an important role in the molecular pathogenesis of amyotrophic lateral sclerosis (ALS). TDP-43 aggregation and translocation out of the nucleus are crucial factors in ALS. T…
View article: LC-MS Peak Assignment Based on Unanimous Selection by Six Machine Learning Algorithms
LC-MS Peak Assignment Based on Unanimous Selection by Six Machine Learning Algorithms Open
Recent Mass spectrometry (MS)-based techniques enable deep proteome coverage with relative quantitative analysis, resulting in increased identification of very weak signals accompanied by increased data size of liquid chromatography (LC)–M…
View article: TGF-β regulates nerve growth factor expression in a mouse intervertebral disc injury model
TGF-β regulates nerve growth factor expression in a mouse intervertebral disc injury model Open
View article: Highly accurate and precise quantification strategy using stable isotope dimethyl labeling coupled with GeLC-MS/MS
Highly accurate and precise quantification strategy using stable isotope dimethyl labeling coupled with GeLC-MS/MS Open
Shotgun proteomics is a powerful method for comprehensively identifying and quantifying tryptic peptides, but it is difficult to analyze proteolytic events. One-dimensional gel and liquid chromatography-tandem mass spectrometry (GeLC-MS/MS…
View article: Age-Dependent Shift of AMPA Receptors From Synapses to Intracellular Compartments in Alzheimer’s Disease: Immunocytochemical Analysis of the CA1 Hippocampal Region in APP/PS1 Transgenic Mouse Model
Age-Dependent Shift of AMPA Receptors From Synapses to Intracellular Compartments in Alzheimer’s Disease: Immunocytochemical Analysis of the CA1 Hippocampal Region in APP/PS1 Transgenic Mouse Model Open
Synapse loss occurs early in Alzheimer's disease (AD) patients and animal models. Alterations at synaptic level are a major morphological correlate of the memory deficits and related symptoms of AD. Given the predominant roles of synaptic …
View article: BRAG2a Mediates mGluR-Dependent AMPA Receptor Internalization at Excitatory Postsynapses through the Interaction with PSD-95 and Endophilin 3
BRAG2a Mediates mGluR-Dependent AMPA Receptor Internalization at Excitatory Postsynapses through the Interaction with PSD-95 and Endophilin 3 Open
Brefeldin A-resistant ArfGEF 2 (BRAG2) [or Iqsec1 (IQ motif and Sec7 domain-containing protein 1)] is a guanine nucleotide exchange factor for ADP ribosylation factor 6 (Arf6), a small GTPase implicated in the membrane trafficking between …
View article: IκB kinase 2 is not essential for platelet activation
IκB kinase 2 is not essential for platelet activation Open
Platelets are small anucleate cells that release a plethora of molecules to ensure functional hemostasis. It has been reported that IκB kinase 2 (IKK2), the central enzyme of the inflammatory NF-κB pathway, is involved in platelet activati…
View article: Advantages of acute brain slices prepared at physiological temperature in characterization of synaptic functions
Advantages of acute brain slices prepared at physiological temperature in characterization of synaptic functions Open
Acute brain slice preparation is a powerful experimental model for investigating the characteristics of synaptic function in the brain. Although brain tissue is usually cut at ice-cold temperature (CT) to facilitate slicing and avoid neuro…
View article: Mice lacking EFA6C/Psd2, a guanine nucleotide exchange factor for Arf6, exhibit lower Purkinje cell synaptic density but normal cerebellar motor functions
Mice lacking EFA6C/Psd2, a guanine nucleotide exchange factor for Arf6, exhibit lower Purkinje cell synaptic density but normal cerebellar motor functions Open
ADP ribosylation factor 6 (Arf6) is a small GTPase that regulates various neuronal events including formation of the axon, dendrites and dendritic spines, and synaptic plasticity through actin cytoskeleton remodeling and endosomal traffick…
View article: Transforming growth factor-β stimulates nerve growth factor production in osteoarthritic synovium
Transforming growth factor-β stimulates nerve growth factor production in osteoarthritic synovium Open
View article: Phosphorylation of SNAP-23 at Ser95 causes a structural alteration and negatively regulates Fc receptor–mediated phagosome formation and maturation in macrophages
Phosphorylation of SNAP-23 at Ser95 causes a structural alteration and negatively regulates Fc receptor–mediated phagosome formation and maturation in macrophages Open
SNAP-23 is a plasma membrane-localized soluble N-ethylmaleimide–sensitive factor attachment protein receptors (SNARE) involved in Fc receptor (FcR)-mediated phagocytosis. However, the regulatory mechanism underlying its function remains el…
View article: CCDC 1539446: Experimental Crystal Structure Determination
CCDC 1539446: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: Maintaining of the Green Fluorescence Emission of 9-Aminoanthracene for Bioimaging Applications
Maintaining of the Green Fluorescence Emission of 9-Aminoanthracene for Bioimaging Applications Open
The green fluorescence emission of 9-aminoanthracence (9AA) was maintained by controlling the oxidation of 9AA with oxygen in the solid state and in solution. The solid-state fluorescence of 9AA was maintained for a lo…
View article: Transforming growth factor activating kinase 1 regulates extracellular matrix degrading enzymes and pain-related molecule expression following tumor necrosis factor-α stimulation of synovial cells: an in vitro study
Transforming growth factor activating kinase 1 regulates extracellular matrix degrading enzymes and pain-related molecule expression following tumor necrosis factor-α stimulation of synovial cells: an in vitro study Open
TAK1 plays an important role in the regulation of TNF-α induced extracellular matrix degrading enzymes and pain-related molecule expression. TAK1 may be a potential target for therapeutic strategies aimed at preventing osteoarthritis progr…
View article: Acceleration of Acid-Catalyzed Hydrolysis in a Biphasic System by Sodium Tetracyanocyclopentadienides
Acceleration of Acid-Catalyzed Hydrolysis in a Biphasic System by Sodium Tetracyanocyclopentadienides Open
The hydrolysis of tert-butyldimethylsilyl L-menthyl ether (3) in a CH2Cl2-1 M HCl biphasic solvent system was accelerated by the addition of sodium tetracyanocyclopentadienides 1. Particularly, the reaction rate was enhanced using sodium s…
View article: Serum pro-BDNF/BDNF as a treatment biomarker for response to docosahexaenoic acid in traumatized people vulnerable to developing psychological distress: a randomized controlled trial
Serum pro-BDNF/BDNF as a treatment biomarker for response to docosahexaenoic acid in traumatized people vulnerable to developing psychological distress: a randomized controlled trial Open
Our open-label pilot study showed that supplementation with docosahexaenoic acid (DHA) increased serum brain-derived neurotrophic factor (BDNF) levels and that there might be an association between changes in serum BDNF levels and reduced …
View article: SNAP-25a/b Isoform Levels in Human Brain Dorsolateral Prefrontal Cortex and Anterior Cingulate Cortex
SNAP-25a/b Isoform Levels in Human Brain Dorsolateral Prefrontal Cortex and Anterior Cingulate Cortex Open
SNAP-25 is a neurotransmitter vesicular docking protein which has been associated with brain disorders such as attention deficit hyperactivity disorder, bipolar disorder and schizophrenia. In this project, we were interested if clinical fa…
View article: Contents Vol. 1, 2015
Contents Vol. 1, 2015 Open