Malcolm Macaulay
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View article: The <i>RAD51</i> paralogue <i>HvXRCC2</i> affects meiosis and recombination in barley
The <i>RAD51</i> paralogue <i>HvXRCC2</i> affects meiosis and recombination in barley Open
Using a positional candidate-gene approach we show that semi-sterile desynaptic8 mutants are associated with deleterious variants of the barley homolog of XRCC2 (X-Ray Repair Cross Complementing 2). In barley XRCC2 mutants, the initial mei…
View article: Environmental DNA Reveals Reykjavík’s Human and Ecological History
Environmental DNA Reveals Reykjavík’s Human and Ecological History Open
Summary Iceland was among the last large islands settled by humans, with colonization (Landnám) in the late 9th century CE (Common Era) and is often portrayed as an ecological disaster driven by the Norse settlers. Here, we revisit this na…
View article: Unlocking the genetic diversity and population structure of the newly introduced two-row spring European HerItage Barley collecTion (ExHIBiT)
Unlocking the genetic diversity and population structure of the newly introduced two-row spring European HerItage Barley collecTion (ExHIBiT) Open
In the last century, breeding programs have traditionally favoured yield-related traits, grown under high-input conditions, resulting in a loss of genetic diversity and an increased susceptibility to stresses in crops. Thus, exploiting und…
View article: The<i>RAD51</i>paralogue<i>HvXRCC2</i>regulates meiosis and recombination in barley
The<i>RAD51</i>paralogue<i>HvXRCC2</i>regulates meiosis and recombination in barley Open
Using a positional candidate-gene approach we show that semi-sterile desynaptic8 mutants are associated with deletions in or complete knockout of the barley homolog of XRCC2 ( X-Ray Repair Cross Complementing 2 ). In barley XRCC2 mutants, …
View article: Loss of E3 ligase HvST1 function substantially increases recombination
Loss of E3 ligase HvST1 function substantially increases recombination Open
During meiosis, genetic recombination occurs via repair of DNA double-strand breaks (DSBs) as crossovers (COs) resulting in the exchange of parental genetic material (De Muyt et al., 2009). Crossovers are important for chromosome segregati…
View article: An Induced Mutation in HvRECQL4 Increases the Overall Recombination and Restores Fertility in a Barley HvMLH3 Mutant Background
An Induced Mutation in HvRECQL4 Increases the Overall Recombination and Restores Fertility in a Barley HvMLH3 Mutant Background Open
Plant breeding relies on the meiotic recombination or crossing over to generate the new combinations of the alleles along and among the chromosomes. However, crossing over is constrained in the crops such as barley by a combination of the …
View article: Downregulation of Barley Regulator of Telomere Elongation Helicase 1 Alters the Distribution of Meiotic Crossovers
Downregulation of Barley Regulator of Telomere Elongation Helicase 1 Alters the Distribution of Meiotic Crossovers Open
Programmed meiotic DNA double-strand breaks (DSBs), necessary for proper chromosomal segregation and viable gamete formation, are repaired by homologous recombination (HR) as crossovers (COs) or non-crossovers (NCOs). The mechanisms regula…
View article: Barley Anther and Meiocyte Transcriptome Dynamics in Meiotic Prophase I
Barley Anther and Meiocyte Transcriptome Dynamics in Meiotic Prophase I Open
In flowering plants, successful germinal cell development and meiotic recombination depend upon a combination of environmental and genetic factors. To gain insights into this specialized reproductive development program we used short- and …
View article: Epigenetic Remodeling of Meiotic Crossover Frequency in Arabidopsis thaliana DNA Methyltransferase Mutants
Epigenetic Remodeling of Meiotic Crossover Frequency in Arabidopsis thaliana DNA Methyltransferase Mutants Open
Meiosis is a specialized eukaryotic cell division that generates haploid gametes required for sexual reproduction. During meiosis, homologous chromosomes pair and undergo reciprocal genetic exchange, termed crossover (CO). Meiotic CO frequ…
View article: Barley sodium content is regulated by natural variants of the Na+ transporter HvHKT1;5
Barley sodium content is regulated by natural variants of the Na+ transporter HvHKT1;5 Open
During plant growth, sodium (Na + ) in the soil is transported via the xylem from the root to the shoot. While excess Na + is toxic to most plants, non-toxic concentrations have been shown to improve crop yields under certain conditions, s…
View article: Time-resolved transcriptome of barley anthers and meiocytes reveals robust and largely stable gene expression changes at meiosis entry
Time-resolved transcriptome of barley anthers and meiocytes reveals robust and largely stable gene expression changes at meiosis entry Open
In flowering plants, successful germinal cell development and meiotic recombination depend upon a combination of environmental and genetic factors. To gain insights into this specialised reproductive development programme we used short- an…
View article: TRA1: A Locus Responsible for Controlling Agrobacterium-Mediated Transformability in Barley
TRA1: A Locus Responsible for Controlling Agrobacterium-Mediated Transformability in Barley Open
In barley (Hordeum vulgare L.), Agrobacterium-mediated transformation efficiency is highly dependent on genotype with very few cultivars being amenable to transformation. Golden Promise is the cultivar most widely used for barley transform…
View article: A Grain of Salt
A Grain of Salt Open
We quantified grain sodium (Na + ) content across a barley GWAS panel grown under optimal conditions. We identified a strong association with a region containing two low and one high Na + accumulating haplotypes of a Class 1 HIGH-AFFINITY …
View article: Supplementary Information Natural variants of HvHKT1;5 regulate sodium content in barley.pdf
Supplementary Information Natural variants of HvHKT1;5 regulate sodium content in barley.pdf Open
Supplementary Information for the manuscript Natural variants of HvHKT1;5 regulate sodium content in barley.Includes Supplementary figures, tables and dataset.
View article: <i>TRA1</i>: a locus responsible for controlling<i>Agrobacterium</i>-mediated transformability in barley
<i>TRA1</i>: a locus responsible for controlling<i>Agrobacterium</i>-mediated transformability in barley Open
In barley ( Hordeum vulgare L.), Agrobacterium -mediated transformation efficiency is highly dependent on genotype with very few cultivars being amenable to transformation. Golden Promise is the cultivar most widely used for barley transfo…
View article: Quantitative trait locus for resistance to the aphid Rhopalosiphum padi L. in barley (Hordeum vulgare L.) is not linked with a genomic region for gramine concentration
Quantitative trait locus for resistance to the aphid Rhopalosiphum padi L. in barley (Hordeum vulgare L.) is not linked with a genomic region for gramine concentration Open
Gramine is an indole alkaloid found in certain grass species. Results of previous studies have diverged as to whether this compound might cause resistance to aphids or not. In a breeding program aiming to introduce resistance to the cereal…
View article: A highly mutagenised barley (cv. Golden Promise) TILLING population coupled with strategies for screening-by-sequencing
A highly mutagenised barley (cv. Golden Promise) TILLING population coupled with strategies for screening-by-sequencing Open
We developed a highly mutagenised barley TILLING population in the transformation competent cultivar Golden Promise. We used novel and cost-efficient screening approaches to successfully identify a broad range of potentially deleterious va…
View article: A Comparison of Mainstream Genotyping Platforms for the Evaluation and Use of Barley Genetic Resources
A Comparison of Mainstream Genotyping Platforms for the Evaluation and Use of Barley Genetic Resources Open
We compared the performance of two commonly used genotyping platforms, genotyping-by-sequencing (GBS) and single nucleotide polymorphism-arrays (SNP), to investigate the extent and pattern of genetic variation within a collection of 1,000 …
View article: desynaptic5 carries a spontaneous semi-dominant mutation affecting Disrupted Meiotic cDNA 1 in barley
desynaptic5 carries a spontaneous semi-dominant mutation affecting Disrupted Meiotic cDNA 1 in barley Open
Despite conservation of the process of meiosis, recombination landscapes vary between species, with large genome grasses such as barley (Hordeum vulgare L.) exhibiting a pattern of recombination that is very heavily skewed to the ends of c…
View article: MOESM3 of A highly mutagenised barley (cv. Golden Promise) TILLING population coupled with strategies for screening-by-sequencing
MOESM3 of A highly mutagenised barley (cv. Golden Promise) TILLING population coupled with strategies for screening-by-sequencing Open
Additional file 3: Table S2. Results of the effects as predicted by SnpEff on the individual transcripts.
View article: Development and Evaluation of a Barley 50k iSelect SNP Array
Development and Evaluation of a Barley 50k iSelect SNP Array Open
High-throughput genotyping arrays continue to be an attractive, cost-effective alternative to sequencing based approaches. We have developed a new 50k Illumina Infinium iSelect genotyping array for barley, a cereal crop species of major in…
View article: Fine mapping of a major QTL for awn length in barley using a multiparent mapping population
Fine mapping of a major QTL for awn length in barley using a multiparent mapping population Open
Awn length was mapped using a multiparent population derived from cv. Morex and four wild accessions. One QTL was fine mapped and candidate genes were identified in NILs by RNA-seq. Barley awns are photosynthetically active and contribute …
View article: A spontaneous mutation in MutL‐Homolog 3 (Hv<scp>MLH</scp>3) affects synapsis and crossover resolution in the barley desynaptic mutant <i>des10</i>
A spontaneous mutation in MutL‐Homolog 3 (Hv<span>MLH</span>3) affects synapsis and crossover resolution in the barley desynaptic mutant <i>des10</i> Open
Summary Although meiosis is evolutionarily conserved, many of the underlying mechanisms show species‐specific differences. These are poorly understood in large genome plant species such as barley ( Hordeum vulgare ) where meiotic recombina…