Manar Shafat
YOU?
Author Swipe
View article: AKT inhibition generates potent polyfunctional clinical grade AUTO1 CAR T-cells, enhancing function and survival
AKT inhibition generates potent polyfunctional clinical grade AUTO1 CAR T-cells, enhancing function and survival Open
Background AUTO1 is a fast off-rate CD19-targeting chimeric antigen receptor (CAR), which has been successfully tested in adult lymphoblastic leukemia. Tscm/Tcm-enriched CAR-T populations confer the best expansion and persistence, but Tscm…
View article: Supplementary Figures 1 and 2 and Supplementary Tables 1 and 2 from MIF-Induced Stromal PKCβ/IL8 Is Essential in Human Acute Myeloid Leukemia
Supplementary Figures 1 and 2 and Supplementary Tables 1 and 2 from MIF-Induced Stromal PKCβ/IL8 Is Essential in Human Acute Myeloid Leukemia Open
This file contains Supplementary Figures and Tables. Supplementary Figure 1 shows long term survival of AML on BM-MSC. Supplementary Figure 2 shows the combinations of AML and BM-MSC co-cultures. Supplementary Table 1 shows AML patient inf…
View article: Supplementary Figures 1 and 2 and Supplementary Tables 1 and 2 from MIF-Induced Stromal PKCβ/IL8 Is Essential in Human Acute Myeloid Leukemia
Supplementary Figures 1 and 2 and Supplementary Tables 1 and 2 from MIF-Induced Stromal PKCβ/IL8 Is Essential in Human Acute Myeloid Leukemia Open
This file contains Supplementary Figures and Tables. Supplementary Figure 1 shows long term survival of AML on BM-MSC. Supplementary Figure 2 shows the combinations of AML and BM-MSC co-cultures. Supplementary Table 1 shows AML patient inf…
View article: Data from MIF-Induced Stromal PKCβ/IL8 Is Essential in Human Acute Myeloid Leukemia
Data from MIF-Induced Stromal PKCβ/IL8 Is Essential in Human Acute Myeloid Leukemia Open
Acute myeloid leukemia (AML) cells exhibit a high level of spontaneous apoptosis when cultured in vitro but have a prolonged survival time in vivo, indicating that tissue microenvironment plays a critical role in promoting AM…
View article: Data from MIF-Induced Stromal PKCβ/IL8 Is Essential in Human Acute Myeloid Leukemia
Data from MIF-Induced Stromal PKCβ/IL8 Is Essential in Human Acute Myeloid Leukemia Open
Acute myeloid leukemia (AML) cells exhibit a high level of spontaneous apoptosis when cultured in vitro but have a prolonged survival time in vivo, indicating that tissue microenvironment plays a critical role in promoting AM…
View article: Pembrolizumab for the treatment of progressive multifocal leukoencephalopathy following anti‐CD19 CAR‐T therapy: a case report
Pembrolizumab for the treatment of progressive multifocal leukoencephalopathy following anti‐CD19 CAR‐T therapy: a case report Open
Progressive multifocal leukoencephalopathy (PML) is an opportunistic brain infection with few treatment options and poor survival when reversal of the underlying immune dysfunction is not achievable. JC polyomavirus reactivation resulting …
View article: International attitudes on COVID-19 vaccination: repeat national cross-sectional surveys across 15 countries
International attitudes on COVID-19 vaccination: repeat national cross-sectional surveys across 15 countries Open
Objective To examine the general public’s views around willingness to receive COVID-19 vaccines and concerns regarding their safety. Design Repeat cross-sectional surveys. Setting Online surveys in Australia, Canada, Denmark, Finland, Fran…
View article: Synthesis of Carboxamide‐Containing Tranylcypromine Analogues as LSD1 (KDM1A) Inhibitors Targeting Acute Myeloid Leukemia
Synthesis of Carboxamide‐Containing Tranylcypromine Analogues as LSD1 (KDM1A) Inhibitors Targeting Acute Myeloid Leukemia Open
Lysine‐specific demethylase 1 (LSD1/KDM1A) oxidatively removes methyl groups from histone proteins, and its aberrant activity has been correlated with cancers including acute myeloid leukemia (AML). We report a novel series of tranylcyprom…
View article: Synthesis of Carboxamides Tranylcypromine Analogues as LSD1 (KDM1A) Inhibitors for AML
Synthesis of Carboxamides Tranylcypromine Analogues as LSD1 (KDM1A) Inhibitors for AML Open
Lysine-specific demethylase 1 (LSD1/KDM1A) oxidatively removes methyl groups from histone proteins and its aberrant activity has been correlated with cancers including acute myeloid leukemia (AML). We report a novel series of tranylcypromi…
View article: Cellular Therapeutic Approaches to Cytomegalovirus Infection Following Allogeneic Stem Cell Transplantation
Cellular Therapeutic Approaches to Cytomegalovirus Infection Following Allogeneic Stem Cell Transplantation Open
Cytomegalovirus (CMV) infection is common following allogeneic hematopoietic stem cell transplant (HSCT) and is a major cause of morbidity and increased mortality. Whilst pharmacotherapy can be effective in the prevention and treatment of …
View article: Synthesis of Carboxamides Tranylcypromine Analogues as LSD1 (KDM1A) Inhibitors for AML
Synthesis of Carboxamides Tranylcypromine Analogues as LSD1 (KDM1A) Inhibitors for AML Open
Lysine-specific demethylase 1 (LSD1/KDM1A) oxidatively removes methyl groups from histone proteins and its aberrant activity has been correlated with cancers including acute myeloid leukemia (AML). We report a novel series of tranylcypromi…
View article: Synthesis of Carboxamides Tranylcypromine Analogues as LSD1 (KDM1A) Inhibitors for AML
Synthesis of Carboxamides Tranylcypromine Analogues as LSD1 (KDM1A) Inhibitors for AML Open
Lysine-specific demethylase 1 (LSD1/KDM1A) oxidatively removes methyl groups from histone proteins and its aberrant activity has been correlated with cancers including acute myeloid leukemia (AML). We report a novel series of tranylcypromi…
View article: Acute myeloid leukemia induces protumoral p16INK4a-driven senescence in the bone marrow microenvironment
Acute myeloid leukemia induces protumoral p16INK4a-driven senescence in the bone marrow microenvironment Open
Acute myeloid leukemia (AML) is an age-related disease that is highly dependent on the bone marrow (BM) microenvironment. With increasing age, tissues accumulate senescent cells, characterized by an irreversible arrest of cell proliferatio…
View article: Myeloma-derived macrophage inhibitory factor regulates bone marrow stromal cell-derived IL-6 via c-MYC
Myeloma-derived macrophage inhibitory factor regulates bone marrow stromal cell-derived IL-6 via c-MYC Open
Our presented findings show that MM-derived MIF causes BMSC secretion of IL-6 and IL-8 via BMSC cMYC. Furthermore, we show that the cMYC inhibitor JQ1 can reduce BMSC secreted IL-6 in vivo, irrespective of tumor burden. These data provide …
View article: HIF1α drives chemokine factor pro-tumoral signaling pathways in acute myeloid leukemia
HIF1α drives chemokine factor pro-tumoral signaling pathways in acute myeloid leukemia Open
View article: Investigation of the role of marrow adipose tissue in the acute myeloid leukaemia bone marrow microenvironment.
Investigation of the role of marrow adipose tissue in the acute myeloid leukaemia bone marrow microenvironment. Open
Acute myeloid leukaemia (AML) is a cancer of the blood forming cells of the bone marrow (BM) and has a high mortality rate in the elderly population. New, tolerable therapeutic strategies are necessary for reducing the mortality rate assoc…
View article: NADPH oxidase-2 derived superoxide drives mitochondrial transfer from bone marrow stromal cells to leukemic blasts
NADPH oxidase-2 derived superoxide drives mitochondrial transfer from bone marrow stromal cells to leukemic blasts Open
Key Points Functional mitochondria are transferred in vivo from BMSC to the leukemic blast. AML-derived NOX2 drives transfer of mitochondria via the generation of superoxide.
View article: Erratum: PIM1 kinase regulates cell death, tumor growth and chemotherapy response in triple-negative breast cancer
Erratum: PIM1 kinase regulates cell death, tumor growth and chemotherapy response in triple-negative breast cancer Open
View article: Inflammatory Differences in Plaque Erosion and Rupture in Patients With ST‐Segment Elevation Myocardial Infarction
Inflammatory Differences in Plaque Erosion and Rupture in Patients With ST‐Segment Elevation Myocardial Infarction Open
Background Plaque erosion causes 30% of ST‐segment elevation myocardial infarctions, but the underlying cause is unknown. Inflammatory infiltrates are less abundant in erosion compared with rupture in autopsy studies. We hypothesized that …
View article: Erratum: PIM1 kinase regulates cell death, tumor growth and chemotherapy response in triple-negative breast cancer
Erratum: PIM1 kinase regulates cell death, tumor growth and chemotherapy response in triple-negative breast cancer Open
View article: The bone marrow microenvironment – Home of the leukemic blasts
The bone marrow microenvironment – Home of the leukemic blasts Open
View article: Leukemic blasts program bone marrow adipocytes to generate a protumoral microenvironment
Leukemic blasts program bone marrow adipocytes to generate a protumoral microenvironment Open
Key Points Bone marrow adipocytes support AML survival. AML induces adipocyte lipolysis of triglyceride to free fatty acids and subsequent transport by FABP4.
View article: MIF-Induced Stromal PKCβ/IL8 Is Essential in Human Acute Myeloid Leukemia
MIF-Induced Stromal PKCβ/IL8 Is Essential in Human Acute Myeloid Leukemia Open
Acute myeloid leukemia (AML) cells exhibit a high level of spontaneous apoptosis when cultured in vitro but have a prolonged survival time in vivo, indicating that tissue microenvironment plays a critical role in promoting AML cell surviva…
View article: PIM1 kinase regulates cell death, tumor growth and chemotherapy response in triple-negative breast cancer
PIM1 kinase regulates cell death, tumor growth and chemotherapy response in triple-negative breast cancer Open
Triple-negative breast cancers (TNBCs) have poor prognosis and lack targeted therapies. Here we identified increased copy number and expression of the PIM1 proto-oncogene in genomic data sets of patients with TNBC. TNBC cells, but not nonm…
View article: UK Breast Cancer Research Symposium 2016: Submitted Abstracts
UK Breast Cancer Research Symposium 2016: Submitted Abstracts Open
View article: Targeting PI3Kδ and PI3Kγ signalling disrupts human AML survival and bone marrow stromal cell mediated protection
Targeting PI3Kδ and PI3Kγ signalling disrupts human AML survival and bone marrow stromal cell mediated protection Open
Phosphoinositide-3-kinase (PI3K) is an enzyme group, known to regulate key survival pathways in acute myeloid leukaemia (AML). It generates phosphatidylinositol-3,4,5-triphosphate, which provides a membrane docking site for protein kinaseB…
View article: Activity of Bruton's tyrosine-kinase inhibitor ibrutinib in patients with CD117-positive acute myeloid leukaemia: a mechanistic study using patient-derived blast cells
Activity of Bruton's tyrosine-kinase inhibitor ibrutinib in patients with CD117-positive acute myeloid leukaemia: a mechanistic study using patient-derived blast cells Open