Manaswini Dash
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View article: CRISPR-GATE: a one-stop repository and guide to computational resources for genome editing experimentation
CRISPR-GATE: a one-stop repository and guide to computational resources for genome editing experimentation Open
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR associated protein (CRISPR-Cas) has emerged and evolved as a revolutionary genome editing technology, transforming research across diverse biological disciplines. Ov…
View article: A miniature alternative to Cas9 and Cas12: Transposon‐associated TnpB mediates targeted genome editing in plants
A miniature alternative to Cas9 and Cas12: Transposon‐associated TnpB mediates targeted genome editing in plants Open
The two popular genome editor nucleases, Cas9 and Cas12a, hypothetically evolved from IscB and TnpB, respectively (Altae-Tran et al., 2021). Recent reports showed that TnpBs also function as RNA-guided nucleases in human cells (Karvelis et…
View article: Optimized protoplast isolation and transfection with a breakpoint: accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot
Optimized protoplast isolation and transfection with a breakpoint: accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot Open
The CRISPR-Cas genome editing tools are revolutionizing agriculture and basic biology with their simplicity and precision ability to modify target genomic loci. Software-predicted guide RNAs (gRNAs) often fail to induce efficient cleavage …
View article: A miniature alternative to Cas9 and Cas12: Transposon-associated TnpB mediates targeted genome editing in plants
A miniature alternative to Cas9 and Cas12: Transposon-associated TnpB mediates targeted genome editing in plants Open
Identifying and engineering RNA-guided genome editing nucleases for size reduction is a highly coveted pursuit aimed at overcoming various bottlenecks. The size reduction not only facilitates effective delivery but also contributes to the …
View article: Optimized protoplast isolation and transfection with a breakpoint: Accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot
Optimized protoplast isolation and transfection with a breakpoint: Accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot Open
The CRISPR-Cas genome editing tools are revolutionizing agriculture and basic biology with their simplicity and ability to modify target genomic loci precisely. Software-predicted guide RNAs (gRNAs) often fail to induce efficient cleavage …
View article: Bioactivity Screening of Thirty Black Turmeric (<i>Curcuma caesia</i> Roxb.) Essential Oils Against Free Radicals and MDR Isolates
Bioactivity Screening of Thirty Black Turmeric (<i>Curcuma caesia</i> Roxb.) Essential Oils Against Free Radicals and MDR Isolates Open
Background Curcuma caesia Roxb. (Black turmeric) is a perennial medicinal herb belonging to the Zingiberaceae family that is endangered in Southeast Asia. It is treasured for its high-quality essential oil with tremendous medicinal and aro…
View article: In-silico structural modelling of cytochrome complex proteins of white turmeric (Curcuma zedoaria)
In-silico structural modelling of cytochrome complex proteins of white turmeric (Curcuma zedoaria) Open
Curcuma zedoaria (Christm.) Roscoe (white turmeric) is a perennial herbaceous plant of family Zingiberaceae and mainly found in the wild areas of tropical and subtropical regions worldwide. The cytochrome proteins in plants play important …
View article: Derivatives of Cinnamic Acid Esters and Terpenic Diversity in Volatiles of Thirty-Six Sand Ginger (Kaempferia galanga L.) Accessions of Eastern India Revealing Quality Chemovars
Derivatives of Cinnamic Acid Esters and Terpenic Diversity in Volatiles of Thirty-Six Sand Ginger (Kaempferia galanga L.) Accessions of Eastern India Revealing Quality Chemovars Open
The essential oil of Kaempferia galanga L. commonly known as sand ginger has increased its demand in national and international market for decades. Cinnamic acid esters like ethyl-p-methoxy cinnamate (EPMC) and ethyl cinnamate (EC) are maj…
View article: Role of sedoheptulose-1,7 bisphosphatase in low light tolerance of rice (Oryza sativa L.)
Role of sedoheptulose-1,7 bisphosphatase in low light tolerance of rice (Oryza sativa L.) Open
View article: DNA as a digital information storage device: hope or hype?
DNA as a digital information storage device: hope or hype? Open
View article: <i>In vitro</i> regeneration of some economically important elite <i>Indica</i> rice genotypes
<i>In vitro</i> regeneration of some economically important elite <i>Indica</i> rice genotypes Open
Rice is an inherent part of human civilization and consumed by majority of Asians as their staple food. In addition to classical plant breeding methods, biotechnological tools including genetic engineering and genome editing are important …