Manfred Rauh
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View article: An isotope dilution-liquid chromatography-tandem mass spectrometry-based candidate reference measurement procedure for the quantification of dehydroepiandrosterone sulfate in human serum and plasma
An isotope dilution-liquid chromatography-tandem mass spectrometry-based candidate reference measurement procedure for the quantification of dehydroepiandrosterone sulfate in human serum and plasma Open
Objectives Dehydroepiandrosterone sulfate (DHEAS), the sulfate ester of dehydroepiandrosterone, is one of the most common steroid hormones in the human body and the precursor of several other androgens. It is primarily used as a diagnostic…
View article: VeRUS: verification of reference intervals based on the uncertainty of sampling
VeRUS: verification of reference intervals based on the uncertainty of sampling Open
Objectives Laboratories are required to routinely verify reported reference intervals (RIs), but common verification methods like the CLSI-EP28-A3c binomial test are often impractical due to sample collection requirements. Indirect verific…
View article: Dietary ω−3 polyunsaturated fatty acids (PUFAs) reduce cholesterol-driven non-small cell lung cancer (NSCLC) progression in mouse models of disease
Dietary ω−3 polyunsaturated fatty acids (PUFAs) reduce cholesterol-driven non-small cell lung cancer (NSCLC) progression in mouse models of disease Open
Background Non-small cell lung cancer (NSCLC) is the leading cause of cancer mortality, and most patients fail to respond to immune checkpoint inhibitors (ICIs). Nutritional factors, particularly dietary lipids, influence cancer progressio…
View article: An isotope dilution-liquid chromatography-tandem mass spectrometry-based candidate reference measurement procedure for the quantification of cortisol in human urine
An isotope dilution-liquid chromatography-tandem mass spectrometry-based candidate reference measurement procedure for the quantification of cortisol in human urine Open
Objectives Precise and accurate determination of urinary cortisol is important for diagnosis and monitoring of cortisol excess and deficiency. To achieve this, a selective and specific, isotope-dilution two-dimensional heart-cut liquid chr…
View article: #1569 Estimation of sodium intake can be improved by correcting 24-hour urinary sodium for 24-hour urine volume
#1569 Estimation of sodium intake can be improved by correcting 24-hour urinary sodium for 24-hour urine volume Open
Background and Aims Long-term sodium balance studies demonstrated that 24-hour urinary sodium excretion does not accurately reflect 24-hour sodium intake, which complicates the use of 24-hour urinary sodium measurements for sodium intake e…
View article: #2429 Body water conservation increases blood pressure independently of Na+ balance
#2429 Body water conservation increases blood pressure independently of Na+ balance Open
Background and Aims In 90%–95% of reported high blood pressure (BP) cases, the underlying cause cannot be determined. While textbook theory suggests that arterial hypertension is caused by sodium and water retention, pre-clinical evidence …
View article: Oral L-arginine cures arginase 1-dependent chronic cutaneous leishmaniasis by redirecting the T helper cell response
Oral L-arginine cures arginase 1-dependent chronic cutaneous leishmaniasis by redirecting the T helper cell response Open
Leishmania (L.) mexicana -induced cutaneous leishmaniasis (CL) is a neglected tropical disease characterized by localized chronic ulcers (LCL) and, in rare cases, by disseminated skin lesions (DCL). The therapeutic options for CL are curre…
View article: Multicenter comparison of LC-MS/MS, radioimmunoassay, and ELISA for assessment of salivary cortisol and testosterone
Multicenter comparison of LC-MS/MS, radioimmunoassay, and ELISA for assessment of salivary cortisol and testosterone Open
LC-MS/MS was the best-performing method for both C and T across all criteria examined. RIA, despite showing slightly higher variance, can still be considered a reliable analytical technique as it met most of the set criteria for C and T. O…
View article: PED-DATA: A Privacy-Preserving Framework for Data-Driven, Pediatric Multi-Center Studies
PED-DATA: A Privacy-Preserving Framework for Data-Driven, Pediatric Multi-Center Studies Open
Introduction: Data-driven analysis of clinical databases is an efficient method for clinical knowledge generation, which is especially suitable when exceptional ethical and practical restrictions apply, such as in pediatrics. In the multi-…
View article: 4-Octyl Itaconate ameliorates diesel exhaust particle-induced oxidative stress in nasal epithelial cells
4-Octyl Itaconate ameliorates diesel exhaust particle-induced oxidative stress in nasal epithelial cells Open
Background and objective Particulate matters such as diesel exhaust particles induce oxidative stress in cells and thereby have a negative impact on health. The aim of this study was to test whether the membrane-permeable, anti-inflammator…
View article: An isotope dilution-liquid chromatography-tandem mass spectrometry-based candidate reference measurement procedure for the quantification of 17β-estradiol in human serum and plasma
An isotope dilution-liquid chromatography-tandem mass spectrometry-based candidate reference measurement procedure for the quantification of 17β-estradiol in human serum and plasma Open
Objectives A new candidate isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)-based reference measurement procedure (RMP) has been developed for the accurate and precise quantification of 17β-estradiol (E2) in hu…
View article: Candidate reference measurement procedure based on isotope dilution-two dimensional-liquid chromatography-tandem mass spectrometry for the quantification of androstenedione in human serum and plasma
Candidate reference measurement procedure based on isotope dilution-two dimensional-liquid chromatography-tandem mass spectrometry for the quantification of androstenedione in human serum and plasma Open
Objectives Androstenedione (ASD) is a biomarker used in the diagnosis of hyperandrogenism and adrenal hyperplasia. Therefore, accurate measurement of serum ASD is pivotal in clinical settings. We aimed to develop a novel highly selective r…
View article: Penalized regression splines in Mixture Density Networks
Penalized regression splines in Mixture Density Networks Open
Mixture Density Networks (MDN) belong to a class of models that can be applied to data which cannot be sufficiently described by a single distribution since it originates from different components of the main unit and therefore needs to be…
View article: An isotope dilution-liquid chromatography-tandem mass spectrometry-based candidate reference measurement procedure for the quantification of cortisone in human serum and plasma
An isotope dilution-liquid chromatography-tandem mass spectrometry-based candidate reference measurement procedure for the quantification of cortisone in human serum and plasma Open
Objectives Cortisone is an inert precursor/metabolite of the potent steroid hormone cortisol. Measurement of serum cortisone levels and the cortisol-cortisone ratio can be useful for the diagnosis of dysfunction in the regulation of cortis…
View article: Adenosine-generating CD39<sup>+</sup>plasmablasts predispose to successful infliximab therapy in pediatric IBD
Adenosine-generating CD39<sup>+</sup>plasmablasts predispose to successful infliximab therapy in pediatric IBD Open
B cells display several immunoregulatory mechanisms including the production of interleukin-10. Ectonucleotidases like CD39 and CD73 influence immune homeostasis by metabolizing eATP and generating immunosuppressive adenosine. The major ob…
View article: Therapeutic drug monitoring of imatinib in paediatric chronic myeloid leukaemia: Data from a real‐world setting
Therapeutic drug monitoring of imatinib in paediatric chronic myeloid leukaemia: Data from a real‐world setting Open
Summary Imatinib (IMA) therapy for paediatric chronic myeloid leukemia (pCML) requires age‐dependent dose adjustments. Assessment of therapeutic drug monitoring (TDM) under ‘real‐world’ conditions was performed. Collection of blood and TDM…
View article: An isotope dilution-liquid chromatography-tandem mass spectrometry-based candidate reference measurement procedure for the quantification of cortisol in human serum and plasma
An isotope dilution-liquid chromatography-tandem mass spectrometry-based candidate reference measurement procedure for the quantification of cortisol in human serum and plasma Open
Objectives Accurate measurement of serum cortisol is crucial for the diagnosis and management of adrenal disorders. Thus, we have developed a novel isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC MS/MS)-based candida…
View article: NFATc1 in CD4+ T cells and CD11c+ dendritic cells drives TH2-mediated eosinophilic inflammation in allergic asthma
NFATc1 in CD4+ T cells and CD11c+ dendritic cells drives TH2-mediated eosinophilic inflammation in allergic asthma Open
View article: Fiber rich food suppressed airway inflammation, GATA3 + Th2 cells, and FcεRIα+ eosinophils in asthma
Fiber rich food suppressed airway inflammation, GATA3 + Th2 cells, and FcεRIα+ eosinophils in asthma Open
Background Allergic Asthma is a disease presenting various endotypes and no current therapies act curative but alleviate disease symptoms. Dietary interventions are gaining increasing importance in regulating immune responses. Furthermore,…
View article: #1429 Daily urinary potassium excretion is not a fixed percentage of daily potassium intake
#1429 Daily urinary potassium excretion is not a fixed percentage of daily potassium intake Open
Background and Aims High potassium intake is associated with a lower blood pressure and lower incidence of chronic kidney disease. Since a significant amount of potassium is lost via stool, the World Health Organization advices to multiply…
View article: Water Conservation Overrides Osmotic Diuresis During SGLT2 Inhibition in Patients With Heart Failure
Water Conservation Overrides Osmotic Diuresis During SGLT2 Inhibition in Patients With Heart Failure Open
Physiological-adaptive water conservation eliminated the expected osmotic diuretic potential of dapagliflozin and thereby prevented a glucose-driven increase in urine volume of approximately 10 mL/kg/d · 75 kg = 750 mL/kg/d. (Hepato-renal …
View article: Imatinib treatment and longitudinal growth in pediatric patients with chronic myeloid leukemia: Influence of demographic, pharmacological, and genetic factors in the German CML-PAED cohort
Imatinib treatment and longitudinal growth in pediatric patients with chronic myeloid leukemia: Influence of demographic, pharmacological, and genetic factors in the German CML-PAED cohort Open
In children and adolescents, impaired growth due to tyrosine kinase inhibitor therapy remains an insufficiently studied adverse effect. This study examines demographic, pharmacological, and genetic factors associated with impaired longitud…
View article: VitaminD3 regulates T cell immune responses in allergen and Rhinovirus induced asthma
VitaminD3 regulates T cell immune responses in allergen and Rhinovirus induced asthma Open
Background: Serum 25(OH)-Vitamin D3 (VitD3) deficiency during infancy has been associated with asthma. The potential therapeutic role of VitD3 given in the airways and its interference with the allergen and Rhinovirus was the objective of …
View article: Report from the HarmoSter study: different LC-MS/MS androstenedione, DHEAS and testosterone methods compare well; however, unifying calibration is a double-edged sword
Report from the HarmoSter study: different LC-MS/MS androstenedione, DHEAS and testosterone methods compare well; however, unifying calibration is a double-edged sword Open
Objectives Current liquid chromatography-tandem mass spectrometry (LC-MS/MS) applications for circulating androgen measurements are technically diverse. Previously, variable results have been reported for testosterone. Data are scarce for …
View article: Routine Surveillance of SARS-CoV-2 Serostatus in Pediatrics Allows Monitoring of Humoral Response
Routine Surveillance of SARS-CoV-2 Serostatus in Pediatrics Allows Monitoring of Humoral Response Open
The occurrence of SARS-CoV-2 infections during the pandemic was mainly based on PCR testing of symptomatic patients. However, with new variants, vaccinations, and the changing of the clinical disease severity, knowledge about general immun…
View article: Evaluation of five multisteroid LC‒MS/MS methods used for routine clinical analysis: comparable performance was obtained for nine analytes
Evaluation of five multisteroid LC‒MS/MS methods used for routine clinical analysis: comparable performance was obtained for nine analytes Open
Objectives A mass spectrometry (LC‒MS/MS)-based interlaboratory comparison study was performed for nine steroid analytes with five participating laboratories. The sample set contained 40 pooled samples of human serum generated from preanal…
View article: A pipeline for the fully automated estimation of continuous reference intervals using real-world data
A pipeline for the fully automated estimation of continuous reference intervals using real-world data Open
View article: Pharmacokinetic–pharmacodynamic modeling of maintenance therapy for childhood acute lymphoblastic leukemia
Pharmacokinetic–pharmacodynamic modeling of maintenance therapy for childhood acute lymphoblastic leukemia Open
In the treatment of childhood acute lymphoblastic leukemia (ALL), current protocols combine initial high-dose multiagent chemotherapy with prolonged oral therapy with 6-mercaptopurine (6MP) and low-dose methotrexate (MTX) maintenance thera…
View article: Frequency and impact of enteric hyperoxaluria in pediatric short bowel syndrome: a retrospective single centre study
Frequency and impact of enteric hyperoxaluria in pediatric short bowel syndrome: a retrospective single centre study Open
Objectives The survival of pediatric patients with short bowel syndrome has improved in recent years. Enteric hyperoxaluria as a pathophysiological consequence has been hardly addressed so far. It can be associated with nephrolithiasis, ne…
View article: Development of a rapid and specific MALDI-TOF mass spectrometric assay for SARS-CoV-2 detection
Development of a rapid and specific MALDI-TOF mass spectrometric assay for SARS-CoV-2 detection Open
We have developed a rapid and highly specific assay for detecting and monitoring SARS-CoV-2 infections by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). As MALDI-TOF mass spectrometers are avai…