Manjuli R. Sharma
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View article: Digital Dystopias: A Study of Modern Literary Thoughts on Privacy, Control, and Commercialization in the Digital Age
Digital Dystopias: A Study of Modern Literary Thoughts on Privacy, Control, and Commercialization in the Digital Age Open
The lines separating the actual world from the virtual have become increasingly hazy in an agewhere digital technology permeates every part of human existence, opening up new potential fordystopian scenarios. This study looks at how writer…
View article: HflX-mediated drug resistance through ribosome splitting and rRNA disordering in mycobacteria
HflX-mediated drug resistance through ribosome splitting and rRNA disordering in mycobacteria Open
HflX is a highly conserved ribosome-associated GTPase implicated in rescuing stalled ribosomes and mediating antibiotic resistance in several bacteria, including macrolide-lincosamide antibiotic resistance in mycobacteria. Mycobacterial Hf…
View article: Drug resistance through ribosome splitting and rRNA disordering in mycobacteria
Drug resistance through ribosome splitting and rRNA disordering in mycobacteria Open
HflX is known to rescue stalled ribosomes and is implicated in antibiotic resistance in several bacteria. Here we present several high-resolution cryo-EM structures of mycobacterial HflX in complex with the ribosome and its 50S subunit, wi…
View article: Non-technical skills for neurosurgeons: An international survey
Non-technical skills for neurosurgeons: An international survey Open
View article: The structure of a hibernating ribosome in a Lyme disease pathogen
The structure of a hibernating ribosome in a Lyme disease pathogen Open
View article: Starvation sensing by mycobacterial RelA/SpoT homologue through constitutive surveillance of translation
Starvation sensing by mycobacterial RelA/SpoT homologue through constitutive surveillance of translation Open
The stringent response, which leads to persistence of nutrient-starved mycobacteria, is induced by activation of the RelA/SpoT homolog (Rsh) upon entry of a deacylated-tRNA in a translating ribosome. However, the mechanism by which Rsh ide…
View article: The structure of a hibernating ribosome in a Lyme disease pathogen
The structure of a hibernating ribosome in a Lyme disease pathogen Open
The spirochete bacterial pathogen Borrelia ( Borreliella) burgdorferi ( Bbu ) affects more than 10% of the world population and causes Lyme disease in about half a million people in the US annually. Therapy for Lyme disease includes antibi…
View article: The small mycobacterial ribosomal protein, bS22, modulates aminoglycoside accessibility to its 16S rRNA helix-44 binding site
The small mycobacterial ribosomal protein, bS22, modulates aminoglycoside accessibility to its 16S rRNA helix-44 binding site Open
Treatment of tuberculosis continues to be challenging due to the widespread latent form of the disease and the emergence of antibiotic-resistant strains of the pathogen, Mycobacterium tuberculosis . Bacterial ribosomes are a common and eff…
View article: Starvation sensing by mycobacterial RelA/SpoT homologue through constitutive surveillance of translation
Starvation sensing by mycobacterial RelA/SpoT homologue through constitutive surveillance of translation Open
The stringent response, which leads to persistence of nutrient-starved mycobacteria, is induced by activation of the RelA/SpoT homologue (Rsh) upon entry of a deacylated-tRNA in a translating ribosome. However, the mechanism by which Rsh i…
View article: Ribosome hibernation: a new molecular framework for targeting nonreplicating persisters of mycobacteria
Ribosome hibernation: a new molecular framework for targeting nonreplicating persisters of mycobacteria Open
Treatment of tuberculosis requires a multi-drug regimen administered for at least 6 months. The long-term chemotherapy is attributed in part to a minor subpopulation of nonreplicating Mycobacterium tuberculosis cells that exhibit phenotypi…
View article: Replacement of S14 Protein in Ribosomes of Zinc-Starved Mycobacteria Reduces Spectinamide Sensitivity
Replacement of S14 Protein in Ribosomes of Zinc-Starved Mycobacteria Reduces Spectinamide Sensitivity Open
Zinc is an essential micronutrient for mycobacteria, and its depletion induces multiple adaptive changes in cellular physiology, the most remarkable of which are remodeling and hibernation of ribosomes. Ribosome remodeling, induced upon re…
View article: Proteomics and electron microscopic characterization of the unusual mitochondrial ribosome-related 45S complex in Leishmania tarentolae
Proteomics and electron microscopic characterization of the unusual mitochondrial ribosome-related 45S complex in Leishmania tarentolae Open
A novel type of ribonucleoprotein (RNP) complex has been described from the kinetoplast-mitochondria of Leishmania tarentolae. The complex, termed the 45S SSU*, contains the 9S small subunit rRNA but does not contain the 12S large subunit …
View article: Structures of the human mitochondrial ribosome bound to EF-G1 reveal distinct features of mitochondrial translation elongation
Structures of the human mitochondrial ribosome bound to EF-G1 reveal distinct features of mitochondrial translation elongation Open
View article: Structural insights into unique features of the human mitochondrial ribosome recycling
Structural insights into unique features of the human mitochondrial ribosome recycling Open
Mammalian mitochondrial ribosomes (mitoribosomes) are responsible for synthesizing proteins that are essential for oxidative phosphorylation (ATP generation). Despite their common ancestry with bacteria, the composition and structure of th…
View article: Structural insights into unique features of the human mitochondrial ribosome recycling
Structural insights into unique features of the human mitochondrial ribosome recycling Open
Mammalian mitochondrial ribosomes (mitoribosomes) are responsible for synthesizing proteins that are essential for oxidative phosphorylation (ATP generation). Despite their common ancestry with bacteria, the composition and structure of th…
View article: Reply to Tobiasson et al.: Zinc depletion is a specific signal for induction of ribosome hibernation in mycobacteria
Reply to Tobiasson et al.: Zinc depletion is a specific signal for induction of ribosome hibernation in mycobacteria Open
We previously reported (1) in PNAS that zinc depletion in Mycobacterium smegmatis leads to reprogramming of ribosomes in which five ribosomal proteins (r-proteins) with a conserved zinc-finger CXXC motif (C+) are replaced by their paralogs…
View article: Structure of Human Mitochondrial Translation Initiation Factor 3 Bound to the Small Ribosomal Subunit
Structure of Human Mitochondrial Translation Initiation Factor 3 Bound to the Small Ribosomal Subunit Open
The human mitochondrial translational initiation factor 3 (IF3mt) carries mitochondrial-specific amino acid extensions at both its N and C termini (N- and C-terminal extensions [NTE and CTE, respectively]), when compared with it…
View article: Structural insights into unique features of the human mitochondrial ribosome recycling
Structural insights into unique features of the human mitochondrial ribosome recycling Open
Mammalian mitochondrial ribosomes (mitoribosomes) are responsible for synthesizing proteins that are essential for oxidative phosphorylation or energy (ATP) generation. Despite their proposed bacterial origin, the composition and structure…
View article: Zinc depletion induces ribosome hibernation in mycobacteria
Zinc depletion induces ribosome hibernation in mycobacteria Open
Significance Mycobacteria as well as other bacteria remodel their ribosomes in response to zinc depletion by replacing zinc-binding ribosomal proteins with zinc-free paralogues, releasing zinc for other metabolic processes. In this study, …
View article: The 55S mammalian mitochondrial ribosome and its tRNA-exit region
The 55S mammalian mitochondrial ribosome and its tRNA-exit region Open