Manuel Bedrossian
YOU?
Author Swipe
View article: Architecture and Field Operation of a Portable Digital Holographic Microscope for In Situ Measurements of Bacterial Motility
Architecture and Field Operation of a Portable Digital Holographic Microscope for In Situ Measurements of Bacterial Motility Open
The development of a next-generation, portable, digital holographic microscope enables the real-time volumetric characterization of bacterial behavior in extreme environments. This microscope is designed to observe bacteria in their native…
View article: Extant life detection using label-free video microscopy in analog aquatic environments
Extant life detection using label-free video microscopy in analog aquatic environments Open
The ability of microbial active motion, morphology, and optical properties to serve as biosignatures was investigated by in situ video microscopy in a wide range of extreme field sites where such imaging had not been performed previously. …
View article: A multi-modal volumetric microscope with automated sample handling for surveying microbial life in liquid samples
A multi-modal volumetric microscope with automated sample handling for surveying microbial life in liquid samples Open
In the study of microbial life, microscopy plays a unique role due to its ability to detect ordered structure, motility, and fluorescence signals. As such it has also recently gained attention in the context of searching for extant life on…
View article: Genetically Encoded Phase Contrast Agents for Digital Holographic Microscopy
Genetically Encoded Phase Contrast Agents for Digital Holographic Microscopy Open
Quantitative phase imaging and digital holographic microscopy have shown great promise for visualizing the motion, structure, and physiology of microorganisms and mammalian cells in three dimensions. However, these imaging techniques curre…
View article: Using the Gouy phase anomaly to localize and track bacteria in digital holographic microscopy 4D images
Using the Gouy phase anomaly to localize and track bacteria in digital holographic microscopy 4D images Open
Described over 100 years ago, the Gouy phase anomaly refers to the additional phase shift that is accumulated as a wave passes through focus. It is potentially useful in analyzing any type of phase-sensitive imaging; in light microscopy,…
View article: ELVIS: A Correlated Light-Field and Digital Holographic Microscope for Field and Laboratory Investigations – Field Demonstration
ELVIS: A Correlated Light-Field and Digital Holographic Microscope for Field and Laboratory Investigations – Field Demonstration Open
View article: Enhancing final image contrast in off-axis digital holography using residual fringes
Enhancing final image contrast in off-axis digital holography using residual fringes Open
We show that background fringe-pattern subtraction is a useful technique for removing static noise from off-axis holographic reconstructions and can enhance image contrast in volumetric reconstructions by an order of magnitude in the case …
View article: Microscopic Object Classification through Passive Motion Observations with Holographic Microscopy
Microscopic Object Classification through Passive Motion Observations with Holographic Microscopy Open
Digital holographic microscopy provides the ability to observe throughout a volume that is large compared to its resolution element without the need to refocus through the volume. This capability enables simultaneous observations of large …
View article: Supplementary document for Using the Gouy phase anomaly to localize and track bacteria in digital holographic microscopy 4D images - 4880204.pdf
Supplementary document for Using the Gouy phase anomaly to localize and track bacteria in digital holographic microscopy 4D images - 4880204.pdf Open
Supplemental figures and code
View article: Genetically encoded phase contrast agents for digital holographic microscopy
Genetically encoded phase contrast agents for digital holographic microscopy Open
Quantitative phase imaging and digital holographic microscopy have shown great promise for visualizing the motion, structure and physiology of microorganisms and mammalian cells in three dimensions. However, these imaging techniques curren…
View article: A machine learning algorithm for identifying and tracking bacteria in three dimensions using Digital Holographic Microscopy
A machine learning algorithm for identifying and tracking bacteria in three dimensions using Digital Holographic Microscopy Open
Digital Holographic Microscopy (DHM) is an emerging technique for three-dimensional imaging of microorganisms due to its high throughput and large depth of field relative to traditional microscopy techniques. While it has shown substantial…
View article: Imaging technologies and strategies for detection of extant extraterrestrial microorganisms
Imaging technologies and strategies for detection of extant extraterrestrial microorganisms Open
There is no reductionist definition of life, so the way organisms look, behave, and move is the most definitive way to identify extraterrestrial life. Life elsewhere in the Solar System is likely to be microbial, but no microscope capable …
View article: Digital Holographic Microscopy, a Method for Detection of Microorganisms in Plume Samples from Enceladus and Other Icy Worlds
Digital Holographic Microscopy, a Method for Detection of Microorganisms in Plume Samples from Enceladus and Other Icy Worlds Open
Detection of extant microbial life on Earth and elsewhere in the Solar System requires the ability to identify and enumerate micrometer-scale, essentially featureless cells. On Earth, bacteria are usually enumerated by culture plating or e…
View article: Sources and propagation of errors in quantitative phase imaging techniques using optical interferometry
Sources and propagation of errors in quantitative phase imaging techniques using optical interferometry Open
Quantitative phase imaging (QPI) has many applications in a broad range of disciplines from astronomy to microbiology. QPI is often performed by optical interferometry, where two coherent beams of light are used to produce interference pat…
View article: Holographic microscopy for 3D tracking of bacteria
Holographic microscopy for 3D tracking of bacteria Open
Understanding when, how, and if bacteria swim is key to understanding critical ecological and biological processes, from carbon cycling to infection. Imaging motility by traditional light microscopy is limited by focus depth, requiring cel…
View article: A Submersible, Off-Axis Holographic Microscope for Detection of Microbial Motility and Morphology in Aqueous and Icy Environments
A Submersible, Off-Axis Holographic Microscope for Detection of Microbial Motility and Morphology in Aqueous and Icy Environments Open
Sea ice is an analog environment for several of astrobiology's near-term targets: Mars, Europa, Enceladus, and perhaps other Jovian or Saturnian moons. Microorganisms, both eukaryotic and prokaryotic, remain active within brine channels in…