Ignacio Ochoa
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View article: Tumor microenvironment in glioblastoma: The central role of the hypoxic–necrotic core
Tumor microenvironment in glioblastoma: The central role of the hypoxic–necrotic core Open
Glioblastoma (GBM), the most aggressive and lethal primary brain tumor, is characterized by profound intratumoral heterogeneity and a hostile tumor microenvironment (TME) that drives immune evasion, therapeutic resistance, and relentless p…
View article: Normothermic Perfusion in Organ Preservation and Evaluation. Promising Future in Kidney Transplantation.
Normothermic Perfusion in Organ Preservation and Evaluation. Promising Future in Kidney Transplantation. Open
Kidney transplantation remains the best treatment for end-stage renal disease. Normothermic Machine Perfusion (NMP) could be a useful tool that could solve the problems that arise from the high demand for organs and their quality, providin…
View article: Microfluidic approaches for liquid biopsy in glioblastoma: Insights into diagnostic and follow‐up strategies
Microfluidic approaches for liquid biopsy in glioblastoma: Insights into diagnostic and follow‐up strategies Open
Glioblastoma (GBM) is a highly malignant brain tumor with a poor survival prognosis of 12–15 months despite current therapeutic strategies. Diagnosing GBM is challenging, often requiring invasive techniques such as tissue biopsy and imagin…
View article: A Novel Multicompartment Barrier‐Free Microfluidic Device Reveals the Impact of Extracellular Matrix Stiffening and Temozolomide on Immune‐Tumor Interactions in Glioblastoma
A Novel Multicompartment Barrier‐Free Microfluidic Device Reveals the Impact of Extracellular Matrix Stiffening and Temozolomide on Immune‐Tumor Interactions in Glioblastoma Open
The immune system plays a crucial role in shaping the glioblastoma tumor microenvironment, characterized by its complexity and dynamic interactions. Understanding the tumor‐immune crosstalk is essential for advancing cancer research and th…
View article: Modelling glioblastoma resistance to temozolomide. A mathematical model to simulate cellular adaptation in vitro
Modelling glioblastoma resistance to temozolomide. A mathematical model to simulate cellular adaptation in vitro Open
Drug resistance is one of the biggest challenges in the fight against cancer. In particular, in the case of glioblastoma, the most lethal brain tumour, resistance to temozolomide (the standard of care drug for chemotherapy in this tumour) …
View article: Advanced Kidney Models In Vitro Using the Established Cell Line Renal Proximal Tubular Epithelial/Telomerase Reverse Transcriptase1 for Nephrotoxicity Assays
Advanced Kidney Models In Vitro Using the Established Cell Line Renal Proximal Tubular Epithelial/Telomerase Reverse Transcriptase1 for Nephrotoxicity Assays Open
Nephrotoxicity stands as one of the most limiting effects in the development and validation of new drugs. The kidney, among the organs evaluated in toxicity assessments, has a higher susceptibility, with nephrotoxic potential frequently ev…
View article: Intestine-On-Chip: Enhancing In Vitro Intestinal Models Using Caco-2 and HT29-MTX Cocultures Under Different Flow Conditions
Intestine-On-Chip: Enhancing In Vitro Intestinal Models Using Caco-2 and HT29-MTX Cocultures Under Different Flow Conditions Open
This study explores the development of in vitro intestinal models on chip using cocultures of Caco-2 and HT29-MTX cells over time under different flow conditions. For this purpose, cocultures in the chip were connected to a syringe pump wi…
View article: Improving Cell-to-Cell and Cell-to-Matrix Contact inside Microphysiological Systems
Improving Cell-to-Cell and Cell-to-Matrix Contact inside Microphysiological Systems Open
Innovative microfluidic technology aims to create more realistic in vitro simulations of human tissues. Organ-on-a-chip (OoC) devices within microfluidic chips mimic in vivo environments but face limitations due to inert material between c…
View article: Characterization by Electrical Impedance of an In Vitro Model Based on Tumor Cell Spheroids
Characterization by Electrical Impedance of an In Vitro Model Based on Tumor Cell Spheroids Open
The main objective of this work is to assess the loosening effect of blebbistatin, a specific inhibitor of myosin II activity, on glioblastoma spheroids. Electric impedance will be used to quantify the effect of this drug, with therapeutic…
View article: Development of an organ-on-chip model for the detection of volatile organic compounds as potential biomarkers of tumour progression
Development of an organ-on-chip model for the detection of volatile organic compounds as potential biomarkers of tumour progression Open
Early detection of tumours remains a significant challenge due to their invasive nature and the limitations of current monitoring techniques. Liquid biopsies have emerged as a minimally invasive diagnostic approach, wherein volatile organi…
View article: Reducing Inert Materials for Optimal Cell–Cell and Cell–Matrix Interactions within Microphysiological Systems
Reducing Inert Materials for Optimal Cell–Cell and Cell–Matrix Interactions within Microphysiological Systems Open
In the pursuit of achieving a more realistic in vitro simulation of human biological tissues, microfluidics has emerged as a promising technology. Organ-on-a-chip (OoC) devices, a product of this technology, contain miniature tissues withi…
View article: In Vitro Growth of Human Follicles: Current and Future Perspectives
In Vitro Growth of Human Follicles: Current and Future Perspectives Open
Ovarian tissue cryopreservation is gaining importance as a successful method to restore fertility to girls and young women at high risk of sterility. However, there are concerns regarding the safety of transplantation after ovarian tissue …
View article: Generation of Self-Induced Myocardial Ischemia in Large-Sized Cardiac Spheroids without Alteration of Environmental Conditions Recreates Fibrotic Remodeling and Tissue Stiffening Revealed by Constriction Assays
Generation of Self-Induced Myocardial Ischemia in Large-Sized Cardiac Spheroids without Alteration of Environmental Conditions Recreates Fibrotic Remodeling and Tissue Stiffening Revealed by Constriction Assays Open
A combination of human-induced pluripotent stem cells (hiPSCs) and 3D microtissue culture techniques allows the generation of models that recapitulate the cardiac microenvironment for preclinical research of new treatments. In particular, …
View article: A microphysiological system for handling graphene related materials under flow conditions
A microphysiological system for handling graphene related materials under flow conditions Open
A kidney-on-a-chip has been developed and optimised to evaluate the effects of exposure to graphene related materials under fluid flow conditions.
View article: Evaluation of gelatin-based hydrogels for colon and pancreas studies using 3D <i>in vitro</i> cell culture
Evaluation of gelatin-based hydrogels for colon and pancreas studies using 3D <i>in vitro</i> cell culture Open
GelMA-based hydrogels with tunable stiffness through two crosslinking strategies and diverse swelling behavior, are evaluated as potential 3D models for CRC and pancreatic cancer. Stiffness and composition influence cell proliferation and …
View article: Tuneable hydrogel patterns in pillarless microfluidic devices
Tuneable hydrogel patterns in pillarless microfluidic devices Open
A novel methodology utilizing plasma surface treatment enables the construction of cell culture chambers featuring abutment-free patterns, facilitating the precise distribution of shear stress.
View article: Modelling glioblastoma resistance to temozolomide. Combination of spheroid and mathematical models to simulate cellular adaptation in vitro
Modelling glioblastoma resistance to temozolomide. Combination of spheroid and mathematical models to simulate cellular adaptation in vitro Open
A bstract Drug resistance is one of the biggest challenges in the fight against cancer. In particular, in the case of glioblastoma, the most lethal brain tumour, resistance to temozolomide (the standard of care drug for chemotherapy in thi…
View article: Nanoparticles Stokes radius assessment through permeability coefficient determination within a new stratified epithelium on-chip model
Nanoparticles Stokes radius assessment through permeability coefficient determination within a new stratified epithelium on-chip model Open
Tissue barrier permeability plays a crucial role in determining the selective transport of substances across epithelial tissues, including drugs, cosmetic substances, and chemicals. The ability of these substances to cross through tissue b…
View article: P14.13.B DECODING GLIOBLASTOMA: SIM4ULATING TUMOUR MICROENVIRONMENT TO DETECT NOVEL BIOMARKERS USING ORGAN-ON-CHIP MODELS
P14.13.B DECODING GLIOBLASTOMA: SIM4ULATING TUMOUR MICROENVIRONMENT TO DETECT NOVEL BIOMARKERS USING ORGAN-ON-CHIP MODELS Open
BACKGROUND Glioblastoma (GBM) is the most common malignant primary brain tumour in adults, with a survival rate of 14 months even after current treatment. There are two major limitations in understanding how the tumour works and what marke…
View article: The Mechanical and Biological Performance of Photopolymerized Gelatin‐Based Hydrogels as a Function of the Reaction Media
The Mechanical and Biological Performance of Photopolymerized Gelatin‐Based Hydrogels as a Function of the Reaction Media Open
From the first experiments with biomaterials to mimic tissue properties, the mechanical and biochemical characterization has evolved extensively. Several properties can be described, however, what should be essential is to conduct a proper…
View article: Metastasis on Chip: Modelling Invasive Breast Cancer Cells
Metastasis on Chip: Modelling Invasive Breast Cancer Cells Open
The purpose of this work is to employ a three-dimensional model to examine breast cancer metastasis from various perspectives. Using a solid breast tumor model exposed to a standard chemotherapeutic treatment scheme, we aim to replicate th…
View article: 3D Biomimetic in Vitro Model of Self-induced Cardiac Ischemia Based on the Co-culture of Cardiomyocytes Derived from hiPSC and Primary Human Cardiac Fibroblast
3D Biomimetic in Vitro Model of Self-induced Cardiac Ischemia Based on the Co-culture of Cardiomyocytes Derived from hiPSC and Primary Human Cardiac Fibroblast Open
Large cardiac spheroids (i.e., > 500 µm diameter) that self-induce an ischemic core were generated by co-culture of cardiomyocytes derived from induced pluripotent cells (hiPSC-CMs) and primary cardiac fibroblast (hCF). Without modificatio…
View article: Impact of tumour microenvironment stiffness on immune cell behaviour using Organ-on-chip models
Impact of tumour microenvironment stiffness on immune cell behaviour using Organ-on-chip models Open
The extracellular matrix (ECM) is one of the main factors that are modified by glioblastoma. The tumour is able to increase the stiffness of the ECM, hindering the infiltration of the immune system into the tumour. Simulating this whole ne…
View article: Performance in Kahoot! activities as predictive of exam performance
Performance in Kahoot! activities as predictive of exam performance Open
Background Game-based learning (GBL) is effective for increasing participation, creativity, and student motivation. However, the discriminative value of GBL for knowledge acquisition has not yet been proven. The aim of this study is to ass…
View article: The Role of Mechanical Properties and Structure of Type I Collagen Hydrogels on Colorectal Cancer Cell Migration
The Role of Mechanical Properties and Structure of Type I Collagen Hydrogels on Colorectal Cancer Cell Migration Open
Mechanical interactions between cells and their microenvironment play an important role in determining cell fate, which is particularly relevant in metastasis, a process where cells invade tissue matrices with different mechanical properti…
View article: Towards Novel Biomimetic In Vitro Models of the Blood–Brain Barrier for Drug Permeability Evaluation
Towards Novel Biomimetic In Vitro Models of the Blood–Brain Barrier for Drug Permeability Evaluation Open
Current available animal and in vitro cell-based models for studying brain-related pathologies and drug evaluation face several limitations since they are unable to reproduce the unique architecture and physiology of the human blood–brain …