Manuel Reithofer
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View article: Establishment of the REMBAC-cassette, a rapid, efficient and manifold BacMam tool for recombinant protein expression
Establishment of the REMBAC-cassette, a rapid, efficient and manifold BacMam tool for recombinant protein expression Open
Efficient recombinant protein production requires mammalian stable cell lines or often relies on inefficient transfection processes. Baculoviral transduction of mammalian cells (BacMam) offers cost-effective and robust gene transfer and st…
View article: Comprehensive Comparison of Baculoviral and Plasmid Gene Delivery in Mammalian Cells
Comprehensive Comparison of Baculoviral and Plasmid Gene Delivery in Mammalian Cells Open
(1) Recombinant protein production in mammalian cells is either based on transient transfection processes, often inefficient and underlying high batch-to-batch variability, or on laborious generation of stable cell lines. Alternatively, Ba…
View article: Verifying antibacterial properties of nanopillars on cicada wings
Verifying antibacterial properties of nanopillars on cicada wings Open
The antibacterial properties of cicada wings originate from hexagonally arranged pillar-like multi-functional nanostructures with species-dependent heights, which are super-hydrophobic and self-cleaning. In the present study, two cicada sp…
View article: A Biomimetic, Silaffin R5-Based Antigen Delivery Platform
A Biomimetic, Silaffin R5-Based Antigen Delivery Platform Open
Nature offers a wide range of evolutionary optimized materials that combine unique properties with intrinsic biocompatibility and that can be exploited as biomimetic materials. The R5 and RRIL peptides employed here are derived from silaff…
View article: Adjuvants and Vaccines Used in Allergen-Specific Immunotherapy Induce Neutrophil Extracellular Traps
Adjuvants and Vaccines Used in Allergen-Specific Immunotherapy Induce Neutrophil Extracellular Traps Open
Aluminum hydroxide (alum) and monophosphoryl-lipid A (MPLA) are conventional adjuvants in vaccines for allergen-specific immunotherapy (AIT). Alum triggers the release of neutrophil extracellular traps (NETs) by neutrophils. NETs contain e…
View article: Human Embryo Models and Drug Discovery
Human Embryo Models and Drug Discovery Open
For obvious reasons, such as, e.g., ethical concerns or sample accessibility, model systems are of highest importance to study the underlying molecular mechanisms of human maladies with the aim to develop innovative and effective therapeut…
View article: 4‐1BB costimulation promotes bystander activation of human CD8 T cells
4‐1BB costimulation promotes bystander activation of human CD8 T cells Open
Costimulatory signals potently promote T‐cell proliferation and effector function. Agonistic antibodies targeting costimulatory receptors of the TNFR family, such as 4‐1BB and CD27, have entered clinical trials in cancer patients. Currentl…
View article: Alum triggers infiltration of human neutrophils ex vivo and causes lysosomal destabilization and mitochondrial membrane potential‐dependent NET‐formation
Alum triggers infiltration of human neutrophils ex vivo and causes lysosomal destabilization and mitochondrial membrane potential‐dependent NET‐formation Open
Aluminium salts have been used in vaccines for decades. However, the mechanisms underlying their adjuvant effect are still unclear. Neutrophils, the first immune cells at the injection site, can release cellular DNA together with granular …
View article: The cytokine environment influence on human skin‐derived T cells
The cytokine environment influence on human skin‐derived T cells Open
Skin resident T cells provide immediate immunologic responses at their specific location and play a role in the pathogenesis of skin diseases such as psoriasis. Recently, IL‐9‐producing T cells were described as a major T‐cell subtype pres…
View article: Alum‐adjuvanted allergoids induce functional IgE‐blocking antibodies
Alum‐adjuvanted allergoids induce functional IgE‐blocking antibodies Open
To the Editor The only therapy that is able to modulate the cause of IgE-mediated allergy and to attain a long-term effect is allergen-specific immunotherapy (AIT). In conventional subcutaneous AIT, the vaccine consists of an extract from …
View article: Allergens with Protease Activity from House Dust Mites
Allergens with Protease Activity from House Dust Mites Open
Globally, house dust mites (HDM) are one of the main sources of allergens causing Type I allergy, which has a high risk of progressing into a severe disabling disease manifestation such as allergic asthma. The strong protease activities of…
View article: Amb a 1 isoforms: Unequal siblings with distinct immunological features
Amb a 1 isoforms: Unequal siblings with distinct immunological features Open
Background Ragweed pollen represents a major allergy risk factor. Ragweed extracts contain five different isoforms of the major allergen Amb a 1. However, the immunological characteristics of Amb a 1 isoforms are not fully investigated. He…