Manuela Carrasquilla
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View article: Continental-scale genomic surveillance of <i>Plasmodium falciparum</i> malaria with rapid nanopore sequencing
Continental-scale genomic surveillance of <i>Plasmodium falciparum</i> malaria with rapid nanopore sequencing Open
In sub-Saharan Africa, continental-scale genomic surveillance of Plasmodium falciparum malaria is needed to track the spread of antimalarial drug resistance and diagnostic test evasion, as well as to monitor parasite evolutionary responses…
View article: Plasmodium falciparum expresses fewer var genes at lower levels during asymptomatic dry season infections than clinical malaria cases
Plasmodium falciparum expresses fewer var genes at lower levels during asymptomatic dry season infections than clinical malaria cases Open
In seasonal transmission areas, clinical malaria occurs during the wet season when mosquitoes are present, while in the dry season, malaria transmission is interrupted and clinical cases are rare. In Mali, Plasmodium falciparum can persist…
View article: Seasonal transcriptional profiling of malaria parasites using a new single-cell atlas of a Malian <i>Plasmodium falciparum</i> isolate
Seasonal transcriptional profiling of malaria parasites using a new single-cell atlas of a Malian <i>Plasmodium falciparum</i> isolate Open
Asymptomatic persistence is crucial for the survival of malaria parasites in seasonal transmission settings, where transmission halts during dry months, while mosquitos are scarce and new infections rare. How Plasmodium falciparum avoids c…
View article: <i>Plasmodium falciparum</i>expresses fewer<i>var</i>genes at lower levels during asymptomatic dry season infections than clinical malaria cases
<i>Plasmodium falciparum</i>expresses fewer<i>var</i>genes at lower levels during asymptomatic dry season infections than clinical malaria cases Open
In seasonal transmission areas, clinical malaria occurs during the wet season when mosquitoes are present, while in the dry season, malaria transmission is interrupted and clinical cases are rare. In Mali, Plasmodium falciparum can persist…
View article: Infection length and host environment influence on Plasmodium falciparum dry season reservoir
Infection length and host environment influence on Plasmodium falciparum dry season reservoir Open
View article: Flexible and cost-effective genomic surveillance of P. falciparum malaria with targeted nanopore sequencing
Flexible and cost-effective genomic surveillance of P. falciparum malaria with targeted nanopore sequencing Open
View article: Resolving drug selection and migration in an inbred South American Plasmodium falciparum population with identity-by-descent analysis
Resolving drug selection and migration in an inbred South American Plasmodium falciparum population with identity-by-descent analysis Open
The human malaria parasite Plasmodium falciparum is globally widespread, but its prevalence varies significantly between and even within countries. Most population genetic studies in P . falciparum focus on regions of high transmission whe…
View article: Barcoding Genetically Distinct Plasmodium falciparum Strains for Comparative Assessment of Fitness and Antimalarial Drug Resistance
Barcoding Genetically Distinct Plasmodium falciparum Strains for Comparative Assessment of Fitness and Antimalarial Drug Resistance Open
The complex interplay between antimalarial resistance and parasite fitness has important implications for understanding the development and spread of drug resistance alleles and the impact of genetic background on transmission. One limitat…
View article: Declines in prevalence alter the optimal level of sexual investment for the malaria parasite <i>Plasmodium falciparum</i>
Declines in prevalence alter the optimal level of sexual investment for the malaria parasite <i>Plasmodium falciparum</i> Open
Successful infectious disease interventions can result in large reductions in parasite prevalence. Such demographic change has fitness implications for individual parasites and may shift the parasite’s optimal life history strategy. Here, …
View article: Design and implementation of multiplexed amplicon sequencing panels to serve genomic epidemiology of infectious disease: A malaria case study
Design and implementation of multiplexed amplicon sequencing panels to serve genomic epidemiology of infectious disease: A malaria case study Open
Multiplexed PCR amplicon sequencing (AmpSeq) is an increasingly popular application for cost‐effective monitoring of threatened species and managed wildlife populations, and shows strong potential for the genomic epidemiology of infectious…
View article: Barcoding genetically distinct<i>Plasmodium falciparum</i>strains for comparative assessment of fitness and antimalarial drug resistance
Barcoding genetically distinct<i>Plasmodium falciparum</i>strains for comparative assessment of fitness and antimalarial drug resistance Open
The repeated emergence of antimalarial drug resistance in Plasmodium falciparum , including to the current frontline antimalarial artemisinin, is a perennial problem for malaria control. Nextgeneration sequencing has greatly accelerated th…
View article: Resolving drug selection and migration in an inbred South American <i>Plasmodium falciparum</i> population with identity-by-descent analysis
Resolving drug selection and migration in an inbred South American <i>Plasmodium falciparum</i> population with identity-by-descent analysis Open
The human malaria parasite Plasmodium falciparum is globally widespread, but its prevalence varies significantly between and even within countries. Most population genetic studies in P. falciparum focus on regions of high transmission wher…
View article: Design and implementation of multiplexed amplicon sequencing panels to serve genomic epidemiology of infectious disease: a malaria case study
Design and implementation of multiplexed amplicon sequencing panels to serve genomic epidemiology of infectious disease: a malaria case study Open
Multiplexed PCR amplicon sequencing (AmpSeq) is an increasingly popular application for cost-effective monitoring of threatened species and managed wildlife populations, and shows strong potential for genomic epidemiology of infectious dis…
View article: Red blood cell tension protects against severe malaria in the Dantu blood group
Red blood cell tension protects against severe malaria in the Dantu blood group Open
View article: Defining multiplicity of vector uptake in transfected Plasmodium parasites
Defining multiplicity of vector uptake in transfected Plasmodium parasites Open
View article: Spatio-temporal dynamics of Plasmodium falciparum transmission within a spatial unit on the Colombian Pacific Coast
Spatio-temporal dynamics of Plasmodium falciparum transmission within a spatial unit on the Colombian Pacific Coast Open
View article: Quantitation of vector uptake reveals non-Poissonian transfection dynamics in <i>Plasmodium falciparum</i>
Quantitation of vector uptake reveals non-Poissonian transfection dynamics in <i>Plasmodium falciparum</i> Open
The recurrent emergence of drug resistance in Plasmodium falciparum increases the urgency to genetically validate drug resistance mechanisms and identify new targets. Reverse genetics have facilitated genome-scale knockout screens in Plasm…
View article: Red blood cell tension controls<i>Plasmodium falciparum</i>invasion and protects against severe malaria in the Dantu blood group
Red blood cell tension controls<i>Plasmodium falciparum</i>invasion and protects against severe malaria in the Dantu blood group Open
Malaria has had a major effect on the human genome, with many protective polymorphisms such as sickle cell trait having been selected to high frequencies in malaria endemic regions 1, 2 . Recently, we showed that a novel blood group varian…
View article: New approaches for measuring fitness of Plasmodium falciparum mutations implicated in drug resistance
New approaches for measuring fitness of Plasmodium falciparum mutations implicated in drug resistance Open
The repeated emergence of drug resistance in Plasmodium falciparum underscores the importance of understanding the genetic architecture of current resistance pathways, as well as any associated fitness costs. Why resistance emerges in part…
View article: Capturing in vivo RNA transcriptional dynamics from the malaria parasite <i>Plasmodium falciparum</i>
Capturing in vivo RNA transcriptional dynamics from the malaria parasite <i>Plasmodium falciparum</i> Open
To capture the transcriptional dynamics within proliferating cells, methods to differentiate nascent transcription from preexisting mRNAs are desired. One approach is to label newly synthesized mRNA transcripts in vivo through the incorpor…
View article: Capturing<i>in vivo</i>RNA transcriptional dynamics from the malaria parasite<i>P. falciparum</i>
Capturing<i>in vivo</i>RNA transcriptional dynamics from the malaria parasite<i>P. falciparum</i> Open
SUMMARY: To capture the transcriptional dynamics within proliferating cells, methods to differentiate nascent transcription from pre-existing mRNAs are desired. One approach is to label newly synthesized mRNA transcripts in vivo through th…
View article: Metabolomic Profiling of the Malaria Box Reveals Antimalarial Target Pathways
Metabolomic Profiling of the Malaria Box Reveals Antimalarial Target Pathways Open
The threat of widespread drug resistance to frontline antimalarials has renewed the urgency for identifying inexpensive chemotherapeutic compounds that are effective against Plasmodium falciparum , the parasite species responsible for the …
View article: Malaria Box compounds with activity in biological assays (malaria, helminths, <i>Wolbachia</i>, and cancer cells) and lacking toxicity at therapeutic levels.
Malaria Box compounds with activity in biological assays (malaria, helminths, <i>Wolbachia</i>, and cancer cells) and lacking toxicity at therapeutic levels. Open
Selectivity Index, SI, is toxicity level/activity level; p, probe-like; d, drug-like.
View article: Antiprotozoal Malaria Box compounds with activity in biological assays and lacking toxicity at therapeutic levels.
Antiprotozoal Malaria Box compounds with activity in biological assays and lacking toxicity at therapeutic levels. Open
Selectivity Index, SI, is toxicity level/activity level; p, probe-like; d, drug-like.
View article: Open Source Drug Discovery with the Malaria Box Compound Collection for Neglected Diseases and Beyond
Open Source Drug Discovery with the Malaria Box Compound Collection for Neglected Diseases and Beyond Open
A major cause of the paucity of new starting points for drug discovery is the lack of interaction between academia and industry. Much of the global resource in biology is present in universities, whereas the focus of medicinal chemistry is…
View article: Open source drug discovery with the malaria box compound collection for neglected diseases and beyond
Open source drug discovery with the malaria box compound collection for neglected diseases and beyond Open
A major cause of the paucity of new starting points for drug discovery is the lack of interaction between academia and industry. Much of the global resource in biology is present in universities, whereas the focus of medicinal chemistry is…