Manuela Gesell Salazar
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View article: Unraveling proteomic chaos by independent component analysis—ClpX proficiency promotes the iron and oxygen limitation responses of <i>Staphylococcus aureus</i> and affects the intracellular bacterial behavior
Unraveling proteomic chaos by independent component analysis—ClpX proficiency promotes the iron and oxygen limitation responses of <i>Staphylococcus aureus</i> and affects the intracellular bacterial behavior Open
In the opportunistic pathogen Staphylococcus aureus , protein homeostasis is largely mediated by the caseinolytic protease (Clp) system. The proteases ClpXP and ClpCP are crucial for general and targeted proteolysis, which rely on the unfo…
View article: Requirement of ClpX for CtsR dissociation from its operator elements upon heat stress in Bacillus subtilis
Requirement of ClpX for CtsR dissociation from its operator elements upon heat stress in Bacillus subtilis Open
A sudden increase in temperature triggers Bacillus subtilis to activate expression of stress-specific heat shock proteins of the CtsR (class three stress gene repressor) regulon to withstand the adverse conditions. Key members of this regu…
View article: Immunoglobulin sub-class levels define inter-donor plasma variability: a longitudinal dual-lab study
Immunoglobulin sub-class levels define inter-donor plasma variability: a longitudinal dual-lab study Open
Advancements in mass spectrometry have transformed plasma proteomics, allowing for high-throughput analysis of large cohorts. This study utilized the TIMES cohort, consisting of 51 healthy participants monitored monthly over 12 months, to …
View article: Unraveling proteomic chaos by independent component analysis - ClpX proficiency promotes the iron and oxygen limitation responses of <i>Staphylococcus aureus</i> and affects the intracellular bacterial behavior
Unraveling proteomic chaos by independent component analysis - ClpX proficiency promotes the iron and oxygen limitation responses of <i>Staphylococcus aureus</i> and affects the intracellular bacterial behavior Open
In the opportunistic pathogen Staphylococcus aureus , protein homeostasis is largely mediated by the Caseinolytic protease (Clp) system. The proteases ClpXP and ClpCP are crucial for general and targeted proteolysis, which rely on the unfo…
View article: Requirement of ClpX for CtsR dissociation from its operator elements upon heat stress in <i>Bacillus subtilis</i>
Requirement of ClpX for CtsR dissociation from its operator elements upon heat stress in <i>Bacillus subtilis</i> Open
A sudden increase in temperature triggers Bacillus subtilis to activate expression of stress-specific heat shock proteins of the CtsR ( c lass t hree s tress gene r epressor) regulon to withstand the adverse conditions. Key members of this…
View article: Integrated genomic and proteomic analysis of the mouse-adapted <i>Staphylococcus aureus</i> strain JSNZ
Integrated genomic and proteomic analysis of the mouse-adapted <i>Staphylococcus aureus</i> strain JSNZ Open
Mouse-adapted Staphylococcus aureus strains have become increasingly relevant in infection research thanks to their ability to better recapitulate clinical infection dynamics in mouse models. However, detailed characterisations required to…
View article: TIE-UP-SIN: a novel method for enhanced identification of protein–protein interactions
TIE-UP-SIN: a novel method for enhanced identification of protein–protein interactions Open
Proteins function through complex interaction networks that govern nearly all aspects of cellular physiology. Identifying protein–protein interactions (PPIs) under native conditions remains challenging due to the transient nature of many c…
View article: Influence of Gestational Age on the Level of Functional Peptides (Peptidome) in Breast Milk
Influence of Gestational Age on the Level of Functional Peptides (Peptidome) in Breast Milk Open
Background/Objectives: Human milk provides essential nutrients and immune factors with beneficial impact on term, but especially preterm infants’ development. Therefore, this study focuses on the quantification of differences in the peptid…
View article: Multi-omics investigation reveals unique markers in Klebsiella pneumoniae compared to closely related species
Multi-omics investigation reveals unique markers in Klebsiella pneumoniae compared to closely related species Open
Introduction The Klebsiella pneumoniae (KP) species complex (KpSC) comprises KP as the predominant species, and six other taxa including two subspecies each of Klebsiella var iicola (KV) and Klebsiella quasipneumoniae (KQ), all capable of …
View article: The global proteome of Streptococcus pneumoniae EF3030 under nutrient-defined in vitro conditions
The global proteome of Streptococcus pneumoniae EF3030 under nutrient-defined in vitro conditions Open
Introduction Streptococcus pneumoniae is a human pathobiont that asymptomatically colonizes the upper respiratory tract but can cause severe diseases such as pneumonia, sepsis, and meningitis, as well as non-invasive infections like otitis…
View article: Linking Virulence and Iron Limitation Response in <i>Staphylococcus aureus</i>: The sRNA IsrR Is Involved in SaeRS Activation
Linking Virulence and Iron Limitation Response in <i>Staphylococcus aureus</i>: The sRNA IsrR Is Involved in SaeRS Activation Open
The Gram-positive opportunistic pathogen Staphylococcus aureus colonizes ∼30% of the human population but also causes various diseases. Precise regulation of genes involved in virulence and metabolic functions is required to adapt t…
View article: The ClpXP protease and the ClpX unfoldase control virulence, cell division, and autolysis in <i>Streptococcus pneumoniae</i>
The ClpXP protease and the ClpX unfoldase control virulence, cell division, and autolysis in <i>Streptococcus pneumoniae</i> Open
In all living cells, molecular chaperones and ATP-dependent proteases are essential for protein homeostasis. The ClpXP protease is a chaperone-protease complex conserved between bacteria and mitochondria. Proteolytic activity resides in th…
View article: pTripleTREP – A vector for tightly controlled expression and purification of virulence factors in Staphylococcus aureus
pTripleTREP – A vector for tightly controlled expression and purification of virulence factors in Staphylococcus aureus Open
View article: Global quantitative proteome analysis of a multi-resistant Klebsiella pneumoniae strain
Global quantitative proteome analysis of a multi-resistant Klebsiella pneumoniae strain Open
Klebsiella pneumoniae is a critical nosocomial pathogen with a rising incidence in antibiotic resistance worldwide. Multidrug-resistant strains of K. pneumoniae pose a major health threat, particularly to immunosuppressed and elderly patie…
View article: Temperature-dependent regulation of bacterial cell division hydrolases by the coordinated action of a regulatory RNA and the ClpXP protease
Temperature-dependent regulation of bacterial cell division hydrolases by the coordinated action of a regulatory RNA and the ClpXP protease Open
A defining feature of bacteria is the peptidoglycan cell wall which provides structural integrity and prevents osmotic lysis. While peptidoglycan hydrolases are required for daughter cell separation, dysregulated cell wall degradation may …
View article: The global proteome of <i>Streptococcus pneumoniae</i> EF3030 under nutrient-defined <i>in vitro</i> conditions
The global proteome of <i>Streptococcus pneumoniae</i> EF3030 under nutrient-defined <i>in vitro</i> conditions Open
Streptococcus pneumoniae is a pathobiont that colonises the upper respiratory tract of humans without causing symptoms but can cause a range of life-threatening diseases including pneumonia, sepsis, and meningitis. It also causes less seve…
View article: SpectroPipeR—a streamlining post Spectronaut® DIA-MS data analysis R package
SpectroPipeR—a streamlining post Spectronaut® DIA-MS data analysis R package Open
Summary Proteome studies frequently encounter challenges in down-stream data analysis due to limited bioinformatics resources, rapid data generation, and variations in analytical methods. To address these issues, we developed SpectroPipeR,…
View article: Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ
Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ Open
View article: Temperature-Dependent Regulation of Bacterial Cell Division Hydrolases by the Coordinated Action of a Regulatory Rna and the Clpxp Protease
Temperature-Dependent Regulation of Bacterial Cell Division Hydrolases by the Coordinated Action of a Regulatory Rna and the Clpxp Protease Open
View article: Activation of the general stress response sigma factor SigB prevents competence development in <i>Bacillus subtilis</i>
Activation of the general stress response sigma factor SigB prevents competence development in <i>Bacillus subtilis</i> Open
Seemingly simple bacteria mount intricate adaptive responses when exposed to physical stress or nutrient limitation, and the activation of these responses is governed by complex signal transduction networks. Upon entry into the stationary …
View article: Functional and Proteomic Dissection of the Contributions of CodY, SigB and the Hibernation Promoting Factor HPF to Interactions of <i>Staphylococcus aureus</i> USA300 with Human Lung Epithelial Cells
Functional and Proteomic Dissection of the Contributions of CodY, SigB and the Hibernation Promoting Factor HPF to Interactions of <i>Staphylococcus aureus</i> USA300 with Human Lung Epithelial Cells Open
Staphylococcus aureus is a leading cause of severe pneumonia. Our recent proteomic investigations into S. aureus invasion of human lung epithelial cells revealed three key adaptive responses: activation of the SigB and CodY r…
View article: Multi-omics investigation reveals unique markers underpinning the success of Klebsiella pneumoniae over closely related species
Multi-omics investigation reveals unique markers underpinning the success of Klebsiella pneumoniae over closely related species Open
Background The Klebsiella pneumoniae (KP) species complex comprises KP as the predominant species, and four others including Klebsiella variicola (KV) and Klebsiella quasipneumoniae (KQ), all capable of causing clinical infections and ofte…
View article: The extracellular serine protease from Staphylococcus epidermidis elicits a type 2-biased immune response in atopic dermatitis patients
The extracellular serine protease from Staphylococcus epidermidis elicits a type 2-biased immune response in atopic dermatitis patients Open
Background Atopic dermatitis (AD) is a chronic, relapsing inflammatory skin disease with skin barrier defects and a misdirected type 2 immune response against harmless antigens. The skin microbiome in AD is characterized by a reduction in …
View article: Reduced interleukin-18 secretion by human monocytic cells in response to infections with hyper-virulent Streptococcus pyogenes
Reduced interleukin-18 secretion by human monocytic cells in response to infections with hyper-virulent Streptococcus pyogenes Open
View article: Multi-omics investigations uncover unique pathogenic markers in clinical<i>Klebsiella pneumoniae</i>that could be leveraged as novel antimicrobial targets
Multi-omics investigations uncover unique pathogenic markers in clinical<i>Klebsiella pneumoniae</i>that could be leveraged as novel antimicrobial targets Open
Background Klebsiella pneumoniae (KP), often multidrug-resistant (MDR), is a significant public health concern and frequently associated with various diseases including urinary-tract infection. In addition, in recent years, an increasing n…
View article: Enhancing bacterial fitness and recombinant enzyme yield by engineering the quality control protease HtrA of <i>Bacillus subtilis</i>
Enhancing bacterial fitness and recombinant enzyme yield by engineering the quality control protease HtrA of <i>Bacillus subtilis</i> Open
Bacillus subtilis is a Gram-positive bacterium that is well-known for its high secretory capacity. Although secretion of homologous proteins is extremely efficient, secretion of heterologous proteins imposes what is known as “secretion str…
View article: MassSpecPreppy—An end‐to‐end solution for automated protein concentration determination and flexible sample digestion for proteomics applications
MassSpecPreppy—An end‐to‐end solution for automated protein concentration determination and flexible sample digestion for proteomics applications Open
In proteomics, fast, efficient, and highly reproducible sample preparation is of utmost importance, particularly in view of fast scanning mass spectrometers enabling analyses of large sample series. To address this need, we have developed …
View article: MassSpecPreppy - an end-to-end solution for automated protein concentration determination and flexible sample digestion for proteomics applications
MassSpecPreppy - an end-to-end solution for automated protein concentration determination and flexible sample digestion for proteomics applications Open
In proteomics, fast, efficient and highly reproducible sample preparation is of utmost importance, particularly in view of fast scanning mass spectrometers enabling analyses of large sample series. To address this need, we have developed t…
View article: Quantitative proteomic analysis of cerebrospinal fluid from patients with idiopathic facial nerve palsy
Quantitative proteomic analysis of cerebrospinal fluid from patients with idiopathic facial nerve palsy Open
Background and purpose Idiopathic facial palsy (IFP) accounts for over 60% of peripheral facial palsy (FP) cases. The cause of IFP remains to be determined. Possible etiologies are nerve swelling due to inflammation and/or viral infection.…
View article: Impact of different oral treatments on the composition of the supragingival plaque microbiome
Impact of different oral treatments on the composition of the supragingival plaque microbiome Open
In conclusion, the mechanisms on plaque biofilm formation of Listerine® and the LPO-system containing lozenges are different. In contrast to Listerine®, the lozenges led to a higher bacterial diversity.