Manuele Giuseppe Muraro
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View article: Dual-Lineage Human 3D Bone Niche Model Reveals Osteoclast-Driven Osteomimicry in Prostate Cancer
Dual-Lineage Human 3D Bone Niche Model Reveals Osteoclast-Driven Osteomimicry in Prostate Cancer Open
Bone is the predominant site of metastasis in advanced prostate cancer (PCa), yet the mechanisms governing tumor-bone interactions remain incompletely understood. A modular human three-dimensional (3D) in vitro bone niche model is develope…
View article: Fibronectin- and Bioactive Glass-Modified Alginate Scaffolds Support Limited Primary Cell Proliferation In Vitro yet Demonstrate Effective Host Integration In Vivo
Fibronectin- and Bioactive Glass-Modified Alginate Scaffolds Support Limited Primary Cell Proliferation In Vitro yet Demonstrate Effective Host Integration In Vivo Open
Alginate-hydroxyapatite (AL) scaffolds modified with fibronectin (FN) or bioactive glass (BGMS10) have recently been characterized for their physicochemical properties and proposed as promising candidates for bone regeneration. Here, we pr…
View article: Fibronectin- and Bioactive Glass-Modified Alginate Scaffolds Support Limited Primary Cell Proliferation In Vitro yet Demonstrate Effective Host Integration In Vivo
Fibronectin- and Bioactive Glass-Modified Alginate Scaffolds Support Limited Primary Cell Proliferation In Vitro yet Demonstrate Effective Host Integration In Vivo Open
Alginate-hydroxyapatite (AL) scaffolds modified with fibronectin (FN), or bioactive glass (BGMS10) have recently been characterized for their physicochemical properties and proposed as promising candidates for bone regeneration. Here, we p…
View article: Perfusion-based ex vivo culture of frozen ovarian cancer tissues with preserved tumor microenvironment
Perfusion-based ex vivo culture of frozen ovarian cancer tissues with preserved tumor microenvironment Open
View article: Tonsil explants as a human in vitro model to study vaccine responses
Tonsil explants as a human in vitro model to study vaccine responses Open
Introduction Vaccination is one of the most effective infection prevention strategies. Viruses with high mutation rates -such as influenza- escape vaccine-induced immunity and represent significant challenges to vaccine design. Influenza v…
View article: Tonsil explants as a human in vitro model to study immune responses to vaccines
Tonsil explants as a human in vitro model to study immune responses to vaccines Open
Vaccination is one of the most effective infection prevention strategies. Viruses with high mutation rates -such as influenza- escape vaccine-induced immunity and represent significant challenges to vaccine design. Influenza vaccine strain…
View article: Bone metastatic niche: Advancing prospective models for investigating prostate cancer and bone microenvironment interactions
Bone metastatic niche: Advancing prospective models for investigating prostate cancer and bone microenvironment interactions Open
View article: A perfusion-based three-dimensional cell culture system to model alveolar rhabdomyosarcoma pathological features
A perfusion-based three-dimensional cell culture system to model alveolar rhabdomyosarcoma pathological features Open
Although a rare disease, rhabdomyosarcoma (RMS) is one of the most common cancers in children the more aggressive and metastatic subtype is the alveolar RMS (ARMS). Survival outcomes with metastatic disease remain dismal and the need for n…
View article: Patient-Derived 3D Models for Preclinical Drug Response Evaluation in Neuroblastoma
Patient-Derived 3D Models for Preclinical Drug Response Evaluation in Neuroblastoma Open
Background Neuroblastoma is the most common solid childhood tumor outside the central nervous system. Metastatic and relapsed disease in neuroblastoma patients still has a poor prognosis. New treatment methods to improve therapy and outcom…
View article: Supplementary Figure 2 from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer
Supplementary Figure 2 from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer Open
Co-culture with PBNs does not impact on growth of CRC cell lines. (A) PBNs from healthy donors (HD) were co-cultured with 6 different CRC cell lines at a CRC: PBN ratio 1: 20, as described in “materials and methodsâ€�. After 72h, tumour …
View article: Legend from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer
Legend from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer Open
Supplementary legend figures
View article: Supplementary Figure 3 from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer
Supplementary Figure 3 from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer Open
CD8+/PBN cross-talk is contact dependent. (A) Cumulative flow-cytometry histograms documenting CD69 expression in CD8+ T cells activated by using different concentrations of surface bound anti-CD3 (TR66, 0.5 and 2μg/ml) and soluble anti C…
View article: Supplementary Table 2 from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer
Supplementary Table 2 from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer Open
Clinical-phatological characteristics of the CRC patient cohort and their association with levels of CD8+ and CD66b+ cell infiltrate.
View article: Supplementary Figure 2 from GM-CSF Production by Tumor Cells Is Associated with Improved Survival in Colorectal Cancer
Supplementary Figure 2 from GM-CSF Production by Tumor Cells Is Associated with Improved Survival in Colorectal Cancer Open
PDF - 480K, CX3CL1/fractalkine gene and protein expression in healthy colon mucosa, CRC and established cell lines.
View article: Supplementary Figure 1 from GM-CSF Production by Tumor Cells Is Associated with Improved Survival in Colorectal Cancer
Supplementary Figure 1 from GM-CSF Production by Tumor Cells Is Associated with Improved Survival in Colorectal Cancer Open
PDF - 216K, Notch Box plot of histoscore values in CRC TMA upon staining with GM-CSF and M-CSF specific reagents.
View article: Supplementary Table 1 from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer
Supplementary Table 1 from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer Open
Clinical-phatological characteristics of the CRC patient cohort and their association with levels of CD66b+ cell infiltrate.
View article: Data from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer
Data from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer Open
Purpose: Tumor infiltration by different T lymphocyte subsets is known to be associated with favorable prognosis in colorectal cancer. Still debated is the role of innate immune system. We investigated clinical relevance, phenotypes…
View article: Data from GM-CSF Production by Tumor Cells Is Associated with Improved Survival in Colorectal Cancer
Data from GM-CSF Production by Tumor Cells Is Associated with Improved Survival in Colorectal Cancer Open
Purpose: Colorectal cancer infiltration by CD16+ myeloid cells correlates with improved prognosis. We addressed mechanistic clues and gene and protein expression of cytokines potentially associated with macrophage polariz…
View article: Supplementary Table 2 from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer
Supplementary Table 2 from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer Open
Clinical-phatological characteristics of the CRC patient cohort and their association with levels of CD8+ and CD66b+ cell infiltrate.
View article: Supplementary Figure 3 from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer
Supplementary Figure 3 from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer Open
CD8+/PBN cross-talk is contact dependent. (A) Cumulative flow-cytometry histograms documenting CD69 expression in CD8+ T cells activated by using different concentrations of surface bound anti-CD3 (TR66, 0.5 and 2μg/ml) and soluble anti C…
View article: Supplementary Figure 2 from GM-CSF Production by Tumor Cells Is Associated with Improved Survival in Colorectal Cancer
Supplementary Figure 2 from GM-CSF Production by Tumor Cells Is Associated with Improved Survival in Colorectal Cancer Open
PDF - 480K, CX3CL1/fractalkine gene and protein expression in healthy colon mucosa, CRC and established cell lines.
View article: Data from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer
Data from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer Open
Purpose: Tumor infiltration by different T lymphocyte subsets is known to be associated with favorable prognosis in colorectal cancer. Still debated is the role of innate immune system. We investigated clinical relevance, phenotypes…
View article: Legend from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer
Legend from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer Open
Supplementary legend figures
View article: Supplementary Figure 2 from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer
Supplementary Figure 2 from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer Open
Co-culture with PBNs does not impact on growth of CRC cell lines. (A) PBNs from healthy donors (HD) were co-cultured with 6 different CRC cell lines at a CRC: PBN ratio 1: 20, as described in “materials and methodsâ€�. After 72h, tumour …
View article: Supplementary Figure 1 from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer
Supplementary Figure 1 from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer Open
Comparative phenotypic analysis of peripheral blood neutrophils from healthy donors and patients with CRC.
View article: Supplementary Figure 1 from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer
Supplementary Figure 1 from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer Open
Comparative phenotypic analysis of peripheral blood neutrophils from healthy donors and patients with CRC.
View article: Supplementary Table 1 from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer
Supplementary Table 1 from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer Open
Clinical-phatological characteristics of the CRC patient cohort and their association with levels of CD66b+ cell infiltrate.
View article: Supplementary Figure 1 from GM-CSF Production by Tumor Cells Is Associated with Improved Survival in Colorectal Cancer
Supplementary Figure 1 from GM-CSF Production by Tumor Cells Is Associated with Improved Survival in Colorectal Cancer Open
PDF - 216K, Notch Box plot of histoscore values in CRC TMA upon staining with GM-CSF and M-CSF specific reagents.
View article: Data from GM-CSF Production by Tumor Cells Is Associated with Improved Survival in Colorectal Cancer
Data from GM-CSF Production by Tumor Cells Is Associated with Improved Survival in Colorectal Cancer Open
Purpose: Colorectal cancer infiltration by CD16+ myeloid cells correlates with improved prognosis. We addressed mechanistic clues and gene and protein expression of cytokines potentially associated with macrophage polariz…
View article: Mini‐ and macro‐scale direct perfusion bioreactors with optimized flow for engineering 3D tissues
Mini‐ and macro‐scale direct perfusion bioreactors with optimized flow for engineering 3D tissues Open
Bioreactors enabling direct perfusion of cell suspensions or culture media through the pores of 3D scaffolds have long been used in tissue engineering to improve cell seeding efficiency as well as uniformity of cell distribution and tissue…