Hai‐Quan Mao
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View article: 819 Nanoscale artificial antigen-presenting cells reveal metabolic interventions modulate clonal composition of CD8 <sup>+</sup> T cells
819 Nanoscale artificial antigen-presenting cells reveal metabolic interventions modulate clonal composition of CD8 <sup>+</sup> T cells Open
View article: Buffer Valency Engineering Enables High-concentration and Shelf-stable DNA Transfection Particles for Viral Vector Production
Buffer Valency Engineering Enables High-concentration and Shelf-stable DNA Transfection Particles for Viral Vector Production Open
Cost-effective and scalable production is critical for advancing the clinical translation of viral vector-mediated gene therapy. The widely used transient transfection method for the production of adeno-associated virus (AAVs) and lentivir…
View article: A multistep platform identifies spleen-tropic lipid nanoparticles for in vivo T cell–targeted delivery of gene-editing proteins
A multistep platform identifies spleen-tropic lipid nanoparticles for in vivo T cell–targeted delivery of gene-editing proteins Open
Lipid nanoparticles (LNPs) are a promising nonviral delivery system for gene-editing proteins, but optimal formulations remain underexplored. Unlike messenger RNA–based approaches, ribonucleoprotein delivery enables immediate genome editin…
View article: Crosslinking of Lipid Nanoparticles Enhances the Delivery Efficiency and Efficacy of mRNA Vaccines
Crosslinking of Lipid Nanoparticles Enhances the Delivery Efficiency and Efficacy of mRNA Vaccines Open
Lipid nanoparticles (LNPs) have enabled the effective delivery of RNA therapeutics and mRNA vaccines. However, their broader applications are limited by the suboptimal stability and endosomal escape efficiency. Here, we present an easy-to-…
View article: Buffer Valency Engineering Enables High-concentration and Shelf-stable DNA Transfection Particles for Viral Vector Production
Buffer Valency Engineering Enables High-concentration and Shelf-stable DNA Transfection Particles for Viral Vector Production Open
Cost-effective and scalable production is critical for advancing the clinical translation of adeno-associated virus (AAV)-mediated gene therapy. The widely used transient transfection method using plasmid DNA (pDNA)-loaded transfection par…
View article: An mRNA lipid nanoparticle-incorporated nanofiber-hydrogel composite for cancer immunotherapy
An mRNA lipid nanoparticle-incorporated nanofiber-hydrogel composite for cancer immunotherapy Open
Hydrogel materials have emerged as versatile platforms for various biomedical applications. Notably, the engineered nanofiber-hydrogel composite (NHC) has proven effective in mimicking the soft tissue extracellular matrix, facilitating sub…
View article: Controlling Payload Heterogeneity in Lipid Nanoparticles for RNA-Based Therapeutics
Controlling Payload Heterogeneity in Lipid Nanoparticles for RNA-Based Therapeutics Open
Lipid nanoparticles (LNPs) are a leading platform for nucleic acid delivery, yet conventional assembly by mixing lipids with RNA yields particles with heterogeneous, bimodal payload distribution. Our transfection experiments demonstrate th…
View article: Engineering the immune and fibrotic response in VML
Engineering the immune and fibrotic response in VML Open
Volumetric muscle loss (VML) provides a significant challenge for regeneration, despite current treatments with free functional muscle transfer. VML injury overwhelms the native process of wound healing, leading to a dysregulated immune re…
View article: Systemic trafficking of mRNA lipid nanoparticle vaccine following intramuscular injection generates potent tissue-specific T cell response
Systemic trafficking of mRNA lipid nanoparticle vaccine following intramuscular injection generates potent tissue-specific T cell response Open
The mRNA lipid nanoparticles (LNPs) represent a new generation of vaccine carriers designed to elicit potent immune responses against infectious diseases and cancer. Despite the clinical success and rapid advancements in mRNA LNP technolog…
View article: mRNA lipid nanoparticle-incorporated nanofiber-hydrogel composite generates a local immunostimulatory niche for cancer immunotherapy
mRNA lipid nanoparticle-incorporated nanofiber-hydrogel composite generates a local immunostimulatory niche for cancer immunotherapy Open
Hydrogel materials have emerged as versatile platforms for various biomedical applications. Notably, the engineered nanofiber-hydrogel composite (NHC) has proven effective in mimicking the soft tissue extracellular matrix, facilitating sub…
View article: Use of polyadenosine tail mimetics to enhance mRNA expression from genes associated with haploinsufficiency disorders
Use of polyadenosine tail mimetics to enhance mRNA expression from genes associated with haploinsufficiency disorders Open
Polyadenosine (poly(A)) tails are nearly ubiquitous in human messenger RNA (mRNA) governing mRNA stability and translation. Crucially, the poly(A) tail regulates cytoplasmic gene expression by undergoing controlled removal upon exposure to…
View article: Ex vivo expansion and hydrogel-mediated in vivo delivery of tissue-resident memory T cells for immunotherapy
Ex vivo expansion and hydrogel-mediated in vivo delivery of tissue-resident memory T cells for immunotherapy Open
Tissue-resident memory T (T RM ) cells preferentially reside in peripheral tissues, serving as key players in tumor immunity and immunotherapy. The lack of effective approaches for expanding T RM cells and delivering these cells in vivo hi…
View article: Granular Nanofiber‐Hydrogel Composite‐Programmed Regenerative Inflammation and Adipose Tissue Formation
Granular Nanofiber‐Hydrogel Composite‐Programmed Regenerative Inflammation and Adipose Tissue Formation Open
The interplay between biomaterials and host immune responses critically determines outcomes in tissue restoration. Recent studies suggest that physicochemical properties of materials can dictate pro‐regenerative versus pro‐fibrotic respons…
View article: The PvRBP2b-TfR1 interaction is not essential for reticulocytes invasion by Plasmodium vivax isolates from Cambodia
The PvRBP2b-TfR1 interaction is not essential for reticulocytes invasion by Plasmodium vivax isolates from Cambodia Open
View article: Selective mycobacterial capture with ultraviolet-polymerized poly-dimethyldiallyl chloride functionalized surfaces
Selective mycobacterial capture with ultraviolet-polymerized poly-dimethyldiallyl chloride functionalized surfaces Open
Tuberculosis (TB) is the top cause of death from a single infectious pathogen after COVID-19. Despite molecular diagnostic advances, two-thirds of the 10 million annual TB cases are still diagnosed using direct smear microscopy which has ~…
View article: Supramolecular assembly of polycation/mRNA nanoparticles and in vivo monocyte programming
Supramolecular assembly of polycation/mRNA nanoparticles and in vivo monocyte programming Open
Size-dependent phagocytosis is a well-characterized phenomenon in monocytes and macrophages. However, this size effect for preferential gene delivery to these important cell targets has not been fully exploited because commonly adopted sta…
View article: Single-Particle Spectroscopic Chromatography Reveals Heterogeneous RNA Loading and Size Correlations in Lipid Nanoparticles
Single-Particle Spectroscopic Chromatography Reveals Heterogeneous RNA Loading and Size Correlations in Lipid Nanoparticles Open
Lipid nanoparticles (LNP) have emerged as pivotal delivery vehicles for RNA therapeutics. Previous research and development usually assumed that LNPs are homogeneous in population, loading density, and composition. Such perspectives are di…
View article: 10. Igf-1 And Agrin Co-delivery Nanoparticles In A Nanofiber Hydrogel-based Drug Delivery System Improve Functional Recovery Following Chronic Denervation
10. Igf-1 And Agrin Co-delivery Nanoparticles In A Nanofiber Hydrogel-based Drug Delivery System Improve Functional Recovery Following Chronic Denervation Open
Purpose: Following peripheral nerve injury, denervated tissues undergo several progressive degenerative processes that impede regeneration and minimize the degree of functional return achievable on reinnervation. Our group has previously d…
View article: 48. Igf-1 Nanoparticles In A Nanofiber Hydrogel-based Drug Delivery System Enhance Functional Recovery In Non-human Primatesfollowing Peripheral Nerve Injury
48. Igf-1 Nanoparticles In A Nanofiber Hydrogel-based Drug Delivery System Enhance Functional Recovery In Non-human Primatesfollowing Peripheral Nerve Injury Open
Purpose: Poor functional recovery remains the leading challenge in peripheral nerve injury (PNI). Insulin-like growth factor 1 (IGF-1) mitigates against the deleterious effects of prolonged denervation on Schwann cells and muscle and promo…
View article: In Vivo Stimulation of Therapeutic Antigen‐Specific T Cells in an Artificial Lymph Node Matrix
In Vivo Stimulation of Therapeutic Antigen‐Specific T Cells in an Artificial Lymph Node Matrix Open
T cells are critical mediators of antigen‐specific immune responses and are common targets for immunotherapy. Biomaterial scaffolds have previously been used to stimulate antigen‐presenting cells to elicit antigen‐specific immune responses…
View article: Liter-scale manufacturing of shelf-stable plasmid DNA/PEI transfection particles for viral vector production
Liter-scale manufacturing of shelf-stable plasmid DNA/PEI transfection particles for viral vector production Open
View article: Screening for lipid nanoparticles that modulate the immune activity of helper T cells towards enhanced antitumour activity
Screening for lipid nanoparticles that modulate the immune activity of helper T cells towards enhanced antitumour activity Open
View article: Machine Learning Elucidates Design Features of Plasmid DNA Lipid Nanoparticles for Cell Type-Preferential Transfection
Machine Learning Elucidates Design Features of Plasmid DNA Lipid Nanoparticles for Cell Type-Preferential Transfection Open
For cell and gene therapies to become more broadly accessible, it is critical to develop and optimize non-viral cell type-preferential gene carriers such as lipid nanoparticles (LNPs). Despite the effectiveness of high throughput screening…
View article: 15. Sustained Agrin Nanoparticle Delivery Improves Neuromuscular Junction Reinnervation and Functional Recovery after Chronic Denervation
15. Sustained Agrin Nanoparticle Delivery Improves Neuromuscular Junction Reinnervation and Functional Recovery after Chronic Denervation Open
PURPOSE: One of the most critical factors contributing to poor outcomes after peripheral nerve injury is the prolonged period of latency prior to reinnervation. Acetylcholine receptors (AChRs) within denervated muscle rapidly destabilize a…
View article: 14. A Tunable Nanoparticle-based Botulinum Toxin Formulation for Extended Duration of Therapeutic Effect
14. A Tunable Nanoparticle-based Botulinum Toxin Formulation for Extended Duration of Therapeutic Effect Open
PURPOSE: Botulinum toxins (BoNT) are increasingly popular neuromodulators with an array of aesthetic and neurological indications. However, these agents are limited by their duration of effect, necessitating frequent redosing. We aim to de…
View article: 60. Insulin Like Growth Factor 1 Nanoparticle Based Drug Delivery System for Peripheral Nerve Injury in Non-human Primates
60. Insulin Like Growth Factor 1 Nanoparticle Based Drug Delivery System for Peripheral Nerve Injury in Non-human Primates Open
PURPOSE: Insulin-like growth factor 1 (IGF-1) reduces the deleterious effects of denervation on Schwann cells and muscle and promotes axonal regeneration. IGF-1 is a small protein with a short half-life of ~5 min, complicating clinical app…
View article: 7. A Mechanically Tunable Protein Nanofiber-hydrogel Composite for Soft Tissue Restoration
7. A Mechanically Tunable Protein Nanofiber-hydrogel Composite for Soft Tissue Restoration Open
PURPOSE: Restoration of soft tissue losses is the sine qua non of plastic surgery, yet there are few off the shelf options that enable immediate volume restoration while encouraging natural soft tissue regeneration over time. Here we explo…
View article: D55. A Tunable Nanoparticle-based Botulinum Toxin Formulation for Extended Duration of Therapeutic Effect
D55. A Tunable Nanoparticle-based Botulinum Toxin Formulation for Extended Duration of Therapeutic Effect Open
PURPOSE: Botulinum toxins (BoNT) are increasingly popular neuromodulators with an array of aesthetic and neurological indications. However, these agents are limited by their duration of effect, necessitating frequent redosing. We aim to de…
View article: D20. Agrin Nanoparticles in a Nanofiber Hydrogel-based Drug Delivery System Improve Neuromuscular Junction Reinnervation following Chronic Denervation
D20. Agrin Nanoparticles in a Nanofiber Hydrogel-based Drug Delivery System Improve Neuromuscular Junction Reinnervation following Chronic Denervation Open
PURPOSE: Following peripheral nerve injury, neuromuscular junctions (NMJs) within denervated muscle rapidly destabilize. Agrin, a proteoglycan essential to NMJ formation, may have an essential role in preserving NMJ receptivity to reinnerv…
View article: High-throughput screening for myelination promoting compounds using human stem cell-derived oligodendrocyte progenitor cells
High-throughput screening for myelination promoting compounds using human stem cell-derived oligodendrocyte progenitor cells Open
Promoting myelination capacity of endogenous oligodendrocyte precursor cells (OPCs) is a promising therapeutic approach for CNS demyelinating disorders such as Multiple Sclerosis (MS). To aid in the discovery of myelination-promoting compo…