Maor Arad
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View article: Development of an Automated, Ultra-Rapid Bottom-Up Proteomics Workflow Utilizing Alginate-Based Hydrogels
Development of an Automated, Ultra-Rapid Bottom-Up Proteomics Workflow Utilizing Alginate-Based Hydrogels Open
A new approach to sample preparation and enzymatic digestion in bottom-up proteomics has been developed using alginate-based hydrogel entrapment of enzymes. This hydrogel facilitates rapid and room-temperature digestions with multienzyme c…
View article: What proteomics has taught us about honey bee ( <i>Apis mellifera</i> ) health and disease
What proteomics has taught us about honey bee ( <i>Apis mellifera</i> ) health and disease Open
The Western honey bee, Apis mellifera , is currently navigating a gauntlet of environmental pressures, including the persistent threat of parasites, pathogens, and climate change – all of which compromise the vitality of honey bee colonies…
View article: Development of automated proteomic workflows utilizing silicon-based coupling agents
Development of automated proteomic workflows utilizing silicon-based coupling agents Open
Automated methods for enzyme immobilization via 4-triethoxysilylbutyraldehyde (TESB) derived silicone-based coupling agents were developed. TESB and its oxidized derivative, 4-triethoxysilylbutanoic acid (TESBA), were determined to be the …
View article: Development of Automated Proteomic Workflows Utilizing Silicon-Based Coupling Agents
Development of Automated Proteomic Workflows Utilizing Silicon-Based Coupling Agents Open
Automated methods for enzyme immobilization via 4-triethoxysilylbutyraldehyde (TESB) derived silicone-based coupling agents were developed. TESB and its oxidized derivative, 4-triethoxysilylbutanoic acid (TESBA), were determined to be the …
View article: Capillary Electrophoresis-Based Immobilized Enzyme Microreactor Utilizing Pepsin for Bottom-Up Proteomics
Capillary Electrophoresis-Based Immobilized Enzyme Microreactor Utilizing Pepsin for Bottom-Up Proteomics Open
In this study, we introduced a novel method for pepsin immobilization through investigation into three different coupling reactions, aiming to simplify peptide mapping. We initially determined the optimal enzyme-to-substrate ratio to be 1:…