Marc Boutry
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View article: Evolutionary and Predictive Functional Insights into the Aquaporin Gene Family in the Allotetraploid Plant Nicotiana tabacum
Evolutionary and Predictive Functional Insights into the Aquaporin Gene Family in the Allotetraploid Plant Nicotiana tabacum Open
Aquaporins (AQPs) are a class of integral membrane proteins that facilitate the membrane diffusion of water and other small solutes. Nicotiana tabacum is an important model plant, and its allotetraploid genome has recently been released, p…
View article: Novel routes towards bioplastics from plants: elucidation of the methylperillate biosynthesis pathway from Salvia dorisiana trichomes
Novel routes towards bioplastics from plants: elucidation of the methylperillate biosynthesis pathway from Salvia dorisiana trichomes Open
Plants produce a large variety of highly functionalized terpenoids. Functional groups such as partially unsaturated rings and carboxyl groups provide handles to use these compounds as feedstock for biobased commodity chemicals. For instanc…
View article: Exploring the N-Glycosylation Profile of Glycoprotein B from Human Cytomegalovirus Expressed in CHO and Nicotiana tabacum BY-2 Cells
Exploring the N-Glycosylation Profile of Glycoprotein B from Human Cytomegalovirus Expressed in CHO and Nicotiana tabacum BY-2 Cells Open
The ability to control the glycosylation pattern of recombinant viral glycoproteins represents a major prerequisite before their use as vaccines. The aim of this study consisted of expressing the large soluble ectodomain of glycoprotein B …
View article: Identification of two new trichome-specific promoters of <i>Nicotiana tabacum</i>
Identification of two new trichome-specific promoters of <i>Nicotiana tabacum</i> Open
Main conclusion pRbcS-T1 and pMALD1 , two new trichome-specific promoters of Nicotiana tabacum , were identified and their strength and specificity were compared to those of previously described promoters in this species. Nicotiana tabacum…
View article: Towards identification of the substrates of ATP-binding cassette transporters
Towards identification of the substrates of ATP-binding cassette transporters Open
Most ATP-binding cassette (ABC) proteins function in transmembrane transport, and plant genomes encode a large number of ABC transporters compared with animal or fungal genomes. These transporters have been classified into eight subfamilie…
View article: The Nicotiana tabacum ABC transporter NtPDR3 secretes O-methylated coumarins in response to iron deficiency
The Nicotiana tabacum ABC transporter NtPDR3 secretes O-methylated coumarins in response to iron deficiency Open
Although iron is present in large amounts in the soil, its poor solubility means that plants have to use various strategies to facilitate its uptake. In this study, we show that expression of NtPDR3/NtABCG3, a Nicotiana tabacum plasma-memb…
View article: Activity of the purified plant ABC transporter NtPDR1 is stimulated by diterpenes and sesquiterpenes involved in constitutive and induced defenses
Activity of the purified plant ABC transporter NtPDR1 is stimulated by diterpenes and sesquiterpenes involved in constitutive and induced defenses Open
Within the plant ATP-binding cassette transporter family, pleiotropic drug resistance (PDR) transporters play essential functions, such as in hormone transport or defense against biotic and abiotic stresses. NtPDR1 from Nicotiana tabacum h…
View article: Plant Glandular Trichomes: Natural Cell Factories of High Biotechnological Interest
Plant Glandular Trichomes: Natural Cell Factories of High Biotechnological Interest Open
Multicellular glandular trichomes are epidermal outgrowths characterized by the presence of a head made of cells that have the ability to secrete or store large quantities of specialized metabolites. Our understanding of the transcriptiona…
View article: Emission of volatile organic compounds from petunia flowers is facilitated by an ABC transporter
Emission of volatile organic compounds from petunia flowers is facilitated by an ABC transporter Open
Active transport of aromas Volatile organic compounds (VOCs) serve as invisible lines of communication among host plants, pathogens, commensals, community groups, and, with flowers, their pollinators. Studying petunia flowers, Adebesin et …
View article: Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy Results in Glycoproteins without Plant-Specific Glycans
Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy Results in Glycoproteins without Plant-Specific Glycans Open
Plants or plant cells can be used to produce pharmacological glycoproteins such as antibodies or vaccines. However these proteins carry N-glycans with plant-typical residues [β(1,2)-xylose and core α(1,3)-fucose], which can greatly impact …
View article: Purification and biochemical characterization of NpABCG5/NpPDR5, a plant pleiotropic drug resistance transporter expressed in <i>Nicotiana tabacum</i> BY-2 suspension cells
Purification and biochemical characterization of NpABCG5/NpPDR5, a plant pleiotropic drug resistance transporter expressed in <i>Nicotiana tabacum</i> BY-2 suspension cells Open
Pleiotropic drug resistance (PDR) transporters belong to the ABCG subfamily of ATP-binding cassette (ABC) transporters and are involved in the transport of various molecules across plasma membranes. During evolution, PDR genes appeared ind…
View article: Photosynthetic Trichomes Contain a Specific Rubisco with a Modified pH-Dependent Activity
Photosynthetic Trichomes Contain a Specific Rubisco with a Modified pH-Dependent Activity Open
Ribulose-1,5-biphosphate carboxylase/oxygenase (Rubisco) is the most abundant enzyme in plants and is responsible for CO2 fixation during photosynthesis. This enzyme is assembled from eight large subunits (RbcL) encoded by a single chlorop…
View article: Gene Inactivation by CRISPR-Cas9 in Nicotiana tabacum BY-2 Suspension Cells
Gene Inactivation by CRISPR-Cas9 in Nicotiana tabacum BY-2 Suspension Cells Open
Plant suspension cells are interesting hosts for the heterologous production of pharmacological proteins such as antibodies. They have the advantage to facilitate the containment and the application of good manufacturing practices. Further…