Marcel Hulst
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View article: Risk assessment of African swine fever transmission by spray-dried porcine plasma in piglet feed and the effect of UV irradiation treatment as an additional safety step
Risk assessment of African swine fever transmission by spray-dried porcine plasma in piglet feed and the effect of UV irradiation treatment as an additional safety step Open
The increase of African swine fever (ASF) outbreaks worldwide has raised concerns about the feeding of spray-dried porcine plasma (SDPP) to pigs. The processing of blood into SDPP should thus guarantee sufficient inactivation of ASF virus …
View article: An interlaboratory proficiency test using metagenomic sequencing as a diagnostic tool for the detection of RNA viruses in swine fecal material
An interlaboratory proficiency test using metagenomic sequencing as a diagnostic tool for the detection of RNA viruses in swine fecal material Open
Metagenomic shotgun sequencing (mNGS) can serve as a generic molecular diagnostic tool. An mNGS proficiency test (PT) was performed in six European veterinary and public health laboratories to detect porcine astroviruses in fecal material …
View article: Cross-Reactivity of Human, Wild Boar, and Farm Animal Sera from Pre- and Post-Pandemic Periods with Alpha- and Βeta-Coronaviruses (CoV), including SARS-CoV-2
Cross-Reactivity of Human, Wild Boar, and Farm Animal Sera from Pre- and Post-Pandemic Periods with Alpha- and Βeta-Coronaviruses (CoV), including SARS-CoV-2 Open
Panels of pre- and post-pandemic farm animals, wild boar and human sera, including human sera able to neutralize SARS-CoV-2 in vitro, were tested in serological tests to determine their cross-reactivity with β- and α-CoV originating from f…
View article: Experimental and field investigations of exposure, replication and transmission of SARS-CoV-2 in pigs in the Netherlands
Experimental and field investigations of exposure, replication and transmission of SARS-CoV-2 in pigs in the Netherlands Open
In order to assess the risk of SARS-CoV-2 infection, transmission and reservoir development in swine, we combined results of an experimental and two observational studies. First, intranasal and intratracheal challenge of eight pigs did not…
View article: Beneficial effects of a plant-fish oil, slow carbohydrate diet on cardio-metabolic health exceed the correcting effects of metformin-pioglitazone in diabetic pigs fed a fast-food diet
Beneficial effects of a plant-fish oil, slow carbohydrate diet on cardio-metabolic health exceed the correcting effects of metformin-pioglitazone in diabetic pigs fed a fast-food diet Open
Background Lifestyle influences endocrine, metabolic and cardiovascular homeostasis. This study investigated the impact of diet and oral anti-diabetic medication on cardio-metabolic health in human-sized diabetic pigs. Methods After a grow…
View article: Occupational and environmental exposure to SARS-CoV-2 in and around infected mink farms
Occupational and environmental exposure to SARS-CoV-2 in and around infected mink farms Open
Objective Unprecedented SARS-CoV-2 infections in farmed minks raised immediate concerns regarding transmission to humans and initiated intensive environmental investigations to assess occupational and environmental exposure. Methods Air sa…
View article: Occupational and environmental exposure to SARS-CoV-2 in and around infected mink farms
Occupational and environmental exposure to SARS-CoV-2 in and around infected mink farms Open
Unprecedented SARS-CoV-2 infections in farmed minks raised immediate concerns regarding human health which initiated intensive environmental investigations. Air sampling was performed in infected mink farms, at farm premises and at residen…
View article: Rapid host response to an infection with Coronavirus. Study of transcriptional responses with Porcine Epidemic Diarrhea Virus
Rapid host response to an infection with Coronavirus. Study of transcriptional responses with Porcine Epidemic Diarrhea Virus Open
The transcriptional response in Vero cells (ATCC ® CCL-81) infected with the coronavirus Porcine Epidemic Diarrhea Virus (PEDV) was measured by RNAseq analysis 4 and 6 hours after infection. Differential expressed genes (DEGs) in PEDV infe…
View article: Study on inactivation of porcine epidemic diarrhoea virus, porcine sapelovirus 1 and adenovirus in the production and storage of laboratory spray‐dried porcine plasma
Study on inactivation of porcine epidemic diarrhoea virus, porcine sapelovirus 1 and adenovirus in the production and storage of laboratory spray‐dried porcine plasma Open
The conditions for inactivation of virus in plasma and SDPP determined are important for producers to inactivate PEDV during production of SDPP.
View article: Additional file 2: of Transcriptional response of cultured porcine intestinal epithelial cells to micro algae extracts in the presence and absence of enterotoxigenic Escherichia coli
Additional file 2: of Transcriptional response of cultured porcine intestinal epithelial cells to micro algae extracts in the presence and absence of enterotoxigenic Escherichia coli Open
DEGs microarray comparisons and functional (pathway) analysis. A). Lists of DEGs used for pathway analysis. B). Enriched pathways without ETEC. C). Enriched pathways with ETEC. (XLSX 392 kb)
View article: Additional file 3: of Transcriptional response of cultured porcine intestinal epithelial cells to micro algae extracts in the presence and absence of enterotoxigenic Escherichia coli
Additional file 3: of Transcriptional response of cultured porcine intestinal epithelial cells to micro algae extracts in the presence and absence of enterotoxigenic Escherichia coli Open
DEGs involved in specific biological processes. A). Regulatory genes. B). Immune effector proteins (EPs). C). NRF2-pathway. D). Beta-oxidation. (XLSX 54 kb)
View article: Full-length genome sequences of porcine epidemic diarrhoea virus strain CV777; Use of NGS to analyse genomic and sub-genomic RNAs
Full-length genome sequences of porcine epidemic diarrhoea virus strain CV777; Use of NGS to analyse genomic and sub-genomic RNAs Open
Porcine epidemic diarrhoea virus, strain CV777, was initially characterized in 1978 as the causative agent of a disease first identified in the UK in 1971. This coronavirus has been widely distributed among laboratories and has been passag…
View article: Additional file 3: of Enrichment of in vivo transcription data from dietary intervention studies with in vitro data provides improved insight into gene regulation mechanisms in the intestinal mucosa
Additional file 3: of Enrichment of in vivo transcription data from dietary intervention studies with in vitro data provides improved insight into gene regulation mechanisms in the intestinal mucosa Open
Table S3. Full names of overlapping DEGs expressed in vivo and in vitro ZnO, rye and amoxicillin interventions. (XLSX 12Â kb)
View article: Additional file 2: of Enrichment of in vivo transcription data from dietary intervention studies with in vitro data provides improved insight into gene regulation mechanisms in the intestinal mucosa
Additional file 2: of Enrichment of in vivo transcription data from dietary intervention studies with in vitro data provides improved insight into gene regulation mechanisms in the intestinal mucosa Open
Table S2. The expression data, and derived list of DEGs, used for functional analysis of the ZnO, rye and amoxicillin in vivo, and IPEC-J2 in vitro interventions. (XLSX 292Â kb)
View article: Nutritional interventions to modulate immune competence in broilers and correlation to quantitative disease phenotype after Necrotic enteritis challenge
Nutritional interventions to modulate immune competence in broilers and correlation to quantitative disease phenotype after Necrotic enteritis challenge Open
This study investigated the effect of four different nutritional interventions, applied during the first week of life, on immune competence parameters of broiler chickens. The four (dietary) interventions were antibiotics in the drinking w…
View article: Effects of Digested Onion Extracts on Intestinal Gene Expression: An Interspecies Comparison Using Different Intestine Models
Effects of Digested Onion Extracts on Intestinal Gene Expression: An Interspecies Comparison Using Different Intestine Models Open
Human intestinal tissue samples are barely accessible to study potential health benefits of nutritional compounds. Numbers of animals used in animal trials, however, need to be minimalized. Therefore, we explored the applicability of in vi…
View article: Effects of a high level of dietary zinc as model intervention on intestinal microbiota and mucosal gene expression in piglets
Effects of a high level of dietary zinc as model intervention on intestinal microbiota and mucosal gene expression in piglets Open
Development of immune competence in pigs is important for resilience, disease resistance, and performance later in life.Dietary interventions in early life can contribute to immune system development.Use of model dietary interventions, suc…