Marcel Mann
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In Vivo Online Monitoring of Intracellular Lipid Accumulation in <i>Ustilago maydis</i> Open
Single cell oils produced in microorganisms constitute appealing alternatives to plant oils. Oleaginous fungi accumulate triacylglycerols in lipid droplets (LD). Their biosynthesis is typically induced under nitrogen limitation. We exploit…
Biomanufacturing Potential of Streamlined Cells Open
A series of Escherichia coli streamlined strains was developed by removing the expression of genes encoding extracellular structures and unessential enzymes. The streamlined strains exhibited improved metabolic performance, including lower…
Fluorescence-Based Online Monitoring Enables Differentiation in Co-Cultures of Untagged Streptomyces Species and Trichoderma reesei Open
Microbial co-cultures provide significant advantages over commonly used axenic cultures in biotechnological processes, including increased productivity and access to novel natural products. However, differentiated quantification of the mic…
Simultaneous online monitoring of viscosity and oxygen transfer rate in shake flask cultures Open
Shake flasks are among the most relevant culture vessels for early-stage process development of viscous microbial cultures. While online process monitoring systems are available for temperature, pH, biomass concentration, dissolved oxygen …
A novel multiparameter sensor for shake flask cultivations: Online biomass, dissolved oxygen, and fluorescence monitoring for comprehensive bioprocess characterization Open
Shake flasks are one of the most widely used cultivation vessels in biotechnological process development. To improve the process understanding, new technologies have been reported for online monitoring of different parameters like oxygen, …
Phase separation behaviour during direct solvent extraction of Corynebacterium glutamicum fermentation broth – Systematic study of crud suppression Open
The economic competitiveness of bio-based production processes is often hindered by the high costs associated with downstream processing, compared to fossil-based methods. Liquid-liquid extraction is a widely used technique for aqueous fer…
Pitfalls in Early Bioprocess Development Using Shake Flask Cultivations Open
For about 100 years, the shake flask has been established for biotechnological cultivations as one of the most important cultivation systems in early process development. Its appeal lies in its simple handling and highly versatile applicat…
Sampling-free investigation of microbial carbon source preferences on renewable feedstocks via online monitoring of oxygen transfer rate Open
The transition towards sustainable bioprocesses requires renewable feedstocks to reduce dependency on finite resources. While plant-based feedstocks offer significant potential, their complex composition poses new challenges. The microorga…
Online monitoring of methane transfer rates unveils nitrogen fixation dynamics in <i>Methylococcus capsulatus</i> Open
This study explores methane utilization by the methanotrophic microorganism Methylococcus capsulatus (Bath) for biomass production, presenting a promising approach to mitigate methane emissions and foster the development sustainable biomat…
Studying microbial triglyceride production from corn stover saccharides unveils insights into the galactose metabolism of Ustilago maydis Open
The global demand for plant oil has reached unprecedented levels and is relevant in all industrial sectors. Driven by the growing awareness for environmental issues of traditional plant oils and the need for eco-friendly alternatives, micr…
Impact of different trace elements on metabolic routes during heterotrophic growth of <span> <i>C. ljungdahlii</i> </span> investigated through online measurement of the carbon dioxide transfer rate Open
Synthesis gas fermentation using acetogenic clostridia is a rapidly increasing research area. It offers the possibility to produce platform chemicals from sustainable C1 carbon sources. The Wood‐Ljungdahl pathway (WLP), which allows acetog…
Online monitoring of gas transfer rates during CO and CO/H<sub>2</sub> gas fermentation in quasi‐continuously ventilated shake flasks Open
Syngas fermentation is a potential player for future emission reduction. The first demonstration and commercial plants have been successfully established. However, due to its novelty, development of syngas fermentation processes is still i…
Online monitoring applying the anaerobic respiratory monitoring system reveals iron(II) limitation in YTF medium for <i>Clostridium ljungdahlii</i> Open
Online monitoring of microbial cultures is an effective approach for studying both aerobic and anaerobic microorganisms. Especially in small‐scale cultivations, several parallel online monitored experiments can generate a detailed understa…
Gas Fermentation Expands the Scope of a Process Network for Material Conversion Open
Biotechnological fermentation is a well‐established process, however, it is far from being fully understood and exploited. A new area of fermentation technology that has evolved over the recent decades is gas fermentation. Many microorgani…
Online measurement of dissolved carbon monoxide concentrations reveals critical operating conditions in gas fermentation experiments Open
Syngas fermentation is one possible contributor to the reduction of greenhouse gas emissions. The conversion of industrial waste gas streams containing CO or H 2 , which are usually combusted, directly reduces the emission of CO 2 into the…