Marcel van den Broek
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View article: New cultivars
New cultivars Open
Sixteen new carnivorous plant cultivars are named and described: Sarracenia 'Ada Rawlings', Sarracenia 'Hans', Sarracenia 'Scream', Sarracenia 'Venom', Sarracenia 'Splatter', Sarracenia 'Cetacean', Dionaea 'Angry Lettuce', Dionaea 'Fiamma'…
View article: Diversity of α-acetolactate decarboxylase in the Saccharomycotina yeast subphylum: From discovery to brewing application
Diversity of α-acetolactate decarboxylase in the Saccharomycotina yeast subphylum: From discovery to brewing application Open
Diacetyl, a vicinal diketone with a low sensory threshold, is a prominent off-flavour in beer, necessitating extended lagering to allow its reduction to non-flavour-active compounds. In brewing, bacterial α-acetolactate decarboxylases are …
View article: Physiology of ethanol-grown Cyberlindnera jadinii: towards sustainable protein production
Physiology of ethanol-grown Cyberlindnera jadinii: towards sustainable protein production Open
View article: Improving CRISPR-Cas9 mediated genome integration in interspecific hybrid yeasts
Improving CRISPR-Cas9 mediated genome integration in interspecific hybrid yeasts Open
View article: Chromosome level genome assembly and annotation of <i>Hanseniaspora mollemarum</i> CBS 18055 strain
Chromosome level genome assembly and annotation of <i>Hanseniaspora mollemarum</i> CBS 18055 strain Open
Hanseniaspora species gained attention due to the ability of these species to ferment simple sugars and to actively contribute to the development of bouquet aromas in wine and cider fermentations. We present a chromosome-level assembly of …
View article: Discovery and Synthetic Applications of a NAD(P)H-Dependent Reductive Aminase from <i>Rhodococcus erythropolis</i>
Discovery and Synthetic Applications of a NAD(P)H-Dependent Reductive Aminase from <i>Rhodococcus erythropolis</i> Open
Reductive amination is one of the most synthetically direct routes to access chiral amines. Several Imine Reductases (IREDs) have been discovered to catalyze reductive amination (Reductive Aminases or RedAms), yet they are dependent on the…
View article: Quantitative physiology and biomass composition of Cyberlindnera jadinii in ethanol-grown cultures
Quantitative physiology and biomass composition of Cyberlindnera jadinii in ethanol-grown cultures Open
Analysis of ethanol-grown batch, chemostat and fed-batch cultures provided a quantitative physiology baseline for fundamental and applied research on C. jadinii. Its high maximum growth rate, high energetic efficiency of ethanol dissimilat…
View article: Quantitative physiology and biomass composition of<i>Cyberlindnera jadinii</i>in ethanol-grown cultures
Quantitative physiology and biomass composition of<i>Cyberlindnera jadinii</i>in ethanol-grown cultures Open
Background Elimination of greenhouse gas emissions in industrial biotechnology requires replacement of carbohydrates by alternative carbon substrates, produced from CO 2 and waste streams. Ethanol is already industrially produced from agri…
View article: Engineering Saccharomyces cerevisiae for fast vitamin-independent aerobic growth
Engineering Saccharomyces cerevisiae for fast vitamin-independent aerobic growth Open
View article: Draft genome sequence of the <i>Saccharomyces cerevisiae Spy</i> Cas9 expressing strain IMX2600, a laboratory and platform strain from the CEN.PK lineage for cell-factory research
Draft genome sequence of the <i>Saccharomyces cerevisiae Spy</i> Cas9 expressing strain IMX2600, a laboratory and platform strain from the CEN.PK lineage for cell-factory research Open
The biobased-economy aims to create a circular biotechnology ecosystem to transition from a fossil fuel-based to a sustainable industry based on biomass. For this, new microbial cell-factories are essential. We present the draft genome of …
View article: Long‐read direct RNA sequencing of the mitochondrial transcriptome of <i>Saccharomyces cerevisiae</i> reveals condition‐dependent intron abundance
Long‐read direct RNA sequencing of the mitochondrial transcriptome of <i>Saccharomyces cerevisiae</i> reveals condition‐dependent intron abundance Open
Mitochondria fulfil many essential roles and have their own genome, which is expressed as polycistronic transcripts that undergo co‐ or posttranscriptional processing and splicing. Due to the inherent complexity and limited technical acces…
View article: CRI-SPA: a high-throughput method for systematic genetic editing of yeast libraries
CRI-SPA: a high-throughput method for systematic genetic editing of yeast libraries Open
Biological functions are orchestrated by intricate networks of interacting genetic elements. Predicting the interaction landscape remains a challenge for systems biology and new research tools allowing simple and rapid mapping of sequence …
View article: Improving CRISPR-Cas9 mediated genome integration in interspecific hybrid yeasts
Improving CRISPR-Cas9 mediated genome integration in interspecific hybrid yeasts Open
View article: The times they are a-changin’
The times they are a-changin’ Open
Opinion about conservation, trade, and sales of carnivorous plants.
View article: Long-read direct RNA sequencing of the mitochondrial transcriptome of<i>Saccharomyces cerevisiae</i>reveals condition-dependent intron turnover
Long-read direct RNA sequencing of the mitochondrial transcriptome of<i>Saccharomyces cerevisiae</i>reveals condition-dependent intron turnover Open
Mitochondria fulfil many essential roles and have their own genome, which is expressed as polycistronic transcripts that undergo co- or post-transcriptional processing and splicing. Due to inherent complexity and limited technical accessib…
View article: CRI-SPA – a mating based CRISPR-Cas9 assisted method for high-throughput genetic modification of yeast strain libraries
CRI-SPA – a mating based CRISPR-Cas9 assisted method for high-throughput genetic modification of yeast strain libraries Open
Biological functions are orchestrated by intricate networks of interacting genetic elements. Predicting the interaction landscape remains a challenge for systems biology and the identification of phenotypic maximas would be of great benefi…
View article: Guidance for dosing and monitoring of oral antineoplastic drugs in breast cancer patients after bariatric surgery
Guidance for dosing and monitoring of oral antineoplastic drugs in breast cancer patients after bariatric surgery Open
Bariatric surgery is a common applied surgical intervention in individuals with obesity to achieve weight-loss, to reduce comorbidities and improve overall survival. However, commonly performed techniques are associated with physiological …
View article: In memory of Allen James Lowrie (1948 – 2021)
In memory of Allen James Lowrie (1948 – 2021) Open
Allen Lowrie memorial.
View article: Modular, synthetic chromosomes as new tools for large scale engineering of metabolism
Modular, synthetic chromosomes as new tools for large scale engineering of metabolism Open
The construction of powerful cell factories requires intensive genetic engineering for the addition of new functionalities and the remodeling of native pathways and processes. The present study demonstrates the feasibility of extensive gen…
View article: Correction to: Class‑II dihydroorotate dehydrogenases from three phylogenetically distant fungi support anaerobic pyrimidine biosynthesis
Correction to: Class‑II dihydroorotate dehydrogenases from three phylogenetically distant fungi support anaerobic pyrimidine biosynthesis Open
Following publication of the original article [1], the authors reported errors in the text of the Results section and in Table 2. It refers to a mutation in a yeast gene as VPS1I410L and to the corresponding change in the Vps1 a…
View article: Class-II dihydroorotate dehydrogenases from three phylogenetically distant fungi support anaerobic pyrimidine biosynthesis
Class-II dihydroorotate dehydrogenases from three phylogenetically distant fungi support anaerobic pyrimidine biosynthesis Open
View article: Modular, synthetic chromosomes as new tools for large scale engineering of metabolism
Modular, synthetic chromosomes as new tools for large scale engineering of metabolism Open
The construction of powerful cell factories requires intensive genetic engineering for the addition of new functionalities and the remodeling of native pathways and processes. The present study demonstrates the feasibility of extensive gen…
View article: Class-II dihydroorotate dehydrogenases from three phylogenetically distant fungi support anaerobic pyrimidine biosynthesis
Class-II dihydroorotate dehydrogenases from three phylogenetically distant fungi support anaerobic pyrimidine biosynthesis Open
Background In most fungi, quinone-dependent Class-II dihydroorotate dehydrogenases (DHODs) are essential for pyrimidine biosynthesis. Coupling of these Class-II DHODHs to mitochondrial respiration makes their in vivo activity dependent on …
View article: Engineering oxygen-independent biotin biosynthesis in Saccharomyces cerevisiae
Engineering oxygen-independent biotin biosynthesis in Saccharomyces cerevisiae Open
View article: Engineering heterologous molybdenum-cofactor-biosynthesis and nitrate-assimilation pathways enables nitrate utilization by Saccharomyces cerevisiae
Engineering heterologous molybdenum-cofactor-biosynthesis and nitrate-assimilation pathways enables nitrate utilization by Saccharomyces cerevisiae Open
View article: Additional file 2 of Class-II dihydroorotate dehydrogenases from three phylogenetically distant fungi support anaerobic pyrimidine biosynthesis
Additional file 2 of Class-II dihydroorotate dehydrogenases from three phylogenetically distant fungi support anaerobic pyrimidine biosynthesis Open
Additional file 2. Protein IDs Ura9 orthologs.
View article: Additional file 1 of Class-II dihydroorotate dehydrogenases from three phylogenetically distant fungi support anaerobic pyrimidine biosynthesis
Additional file 1 of Class-II dihydroorotate dehydrogenases from three phylogenetically distant fungi support anaerobic pyrimidine biosynthesis Open
Additional file 1. Protein sequences Ura9 orthologs.
View article: Additional file 4 of Class-II dihydroorotate dehydrogenases from three phylogenetically distant fungi support anaerobic pyrimidine biosynthesis
Additional file 4 of Class-II dihydroorotate dehydrogenases from three phylogenetically distant fungi support anaerobic pyrimidine biosynthesis Open
Additional file 4. Codon-optimized protein sequences.
View article: Additional file 3 of Class-II dihydroorotate dehydrogenases from three phylogenetically distant fungi support anaerobic pyrimidine biosynthesis
Additional file 3 of Class-II dihydroorotate dehydrogenases from three phylogenetically distant fungi support anaerobic pyrimidine biosynthesis Open
Additional file 3. Raw phylogenetic tree.
View article: A supernumerary designer chromosome for modular <i>in vivo</i> pathway assembly in <i>Saccharomyces cerevisiae</i>
A supernumerary designer chromosome for modular <i>in vivo</i> pathway assembly in <i>Saccharomyces cerevisiae</i> Open
The construction of microbial cell factories for sustainable production of chemicals and pharmaceuticals requires extensive genome engineering. Using Saccharomyces cerevisiae, this study proposes synthetic neochromosomes as orthogonal expr…