Marcin Grabowicz
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View article: LPS O-antigen polysaccharide length impacts outer membrane permeability of enteric gram-negative bacteria
LPS O-antigen polysaccharide length impacts outer membrane permeability of enteric gram-negative bacteria Open
The gram-negative outer membrane (OM) forms the bacterial cell surface and acts as a barrier against antibiotic influx. In enteric species, the OM is covered by lipopolysaccharides (LPS) decorated with varying lengths of O-antigen (O-Ag) p…
View article: LPS O-antigen polysaccharide length impacts outer membrane permeability of enteric gram-negative bacteria
LPS O-antigen polysaccharide length impacts outer membrane permeability of enteric gram-negative bacteria Open
The Gram-negative outer membrane (OM) forms the bacterial cell surface and acts as a barrier against antibiotic influx. In enteric species, the OM is covered by lipopolysaccharides (LPS) decorated with varying lengths of O-antigen (O-Ag) p…
View article: Antitoxin control of optimal transcriptional repression in the atypical HigB–HigA toxin–antitoxin system from <i>Proteus vulgaris</i>
Antitoxin control of optimal transcriptional repression in the atypical HigB–HigA toxin–antitoxin system from <i>Proteus vulgaris</i> Open
Bacterial toxin–antitoxin (TA) pairs transcriptionally autoregulate their expression via a repression/derepression mechanism in response to changing environmental conditions. The structural diversity of TA systems influences the mechanisms…
View article: Repurposing a drug to punish carbapenem-resistant <i>Acinetobacter baumannii</i>
Repurposing a drug to punish carbapenem-resistant <i>Acinetobacter baumannii</i> Open
The OXA β-lactamases in Acinetobacter baumannii represent a primary mechanism for resistance to the carbapenems, a class of antibiotics that represent a last line for treatment. In a screen of an U.S. Food and Drug Administration (FDA)-app…
View article: Outer membrane lipoproteins: late to the party, but the center of attention
Outer membrane lipoproteins: late to the party, but the center of attention Open
An outer membrane (OM) is the hallmark feature that is often used to distinguish “Gram-negative” bacteria. Our understanding of how the OM is built rests largely on studies of Escherichia coli . In that organism—and seemingly in all specie…
View article: The BfmRS stress response protects <i>Acinetobacter baumannii</i> against defects in outer membrane lipoprotein biogenesis
The BfmRS stress response protects <i>Acinetobacter baumannii</i> against defects in outer membrane lipoprotein biogenesis Open
The outer membrane (OM) of Gram-negative bacteria is the outermost layer of the cell and serves as permeability barrier against environmental toxins, including antibiotics. The OM is built by several pathways that transport and assemble li…
View article: Genetic analysis reveals a robust and hierarchical recruitment of the LolA chaperone to the LolCDE lipoprotein transporter
Genetic analysis reveals a robust and hierarchical recruitment of the LolA chaperone to the LolCDE lipoprotein transporter Open
Resistance to current antibiotics is increasingly common. New antibiotics that target essential processes are needed to expand clinical options. For Gram-negative bacteria, their cell surface—the outer membrane (OM)—is an essential organel…
View article: Genetic analysis reveals a robust and hierarchical recruitment of the LolA chaperone to the LolCDE lipoprotein transporter
Genetic analysis reveals a robust and hierarchical recruitment of the LolA chaperone to the LolCDE lipoprotein transporter Open
The outer membrane (OM) is an essential organelle of Gram-negative bacteria. Lipoproteins are key to building the OM, performing essential functions in several OM assembly machines. Lipoproteins mature in the inner membrane (IM) and are th…
View article: Molecular insights into <i>Escherichia coli</i> Cpx envelope stress response activation by the sensor lipoprotein <scp>Nl</scp>p<scp>E</scp>
Molecular insights into <i>Escherichia coli</i> Cpx envelope stress response activation by the sensor lipoprotein <span>Nl</span>p<span>E</span> Open
Bacterial two‐component signal transduction systems provide sensory inputs for appropriately adapting gene expression. These systems rely on a histidine kinase that phosphorylates a response regulator which alters gene expression. Several …
View article: Teasing apart the evolution of lipoprotein trafficking in gram-negative bacteria reveals a bifunctional LolA
Teasing apart the evolution of lipoprotein trafficking in gram-negative bacteria reveals a bifunctional LolA Open
The outer membrane (OM) is the defining feature of gram-negative bacteria and is an essential organelle. Accordingly, OM assembly pathways and their essential protein components are conserved throughout all gram-negative species. Lipoprote…
View article: A Biological Signature for the Inhibition of Outer Membrane Lipoprotein Biogenesis
A Biological Signature for the Inhibition of Outer Membrane Lipoprotein Biogenesis Open
Gram-negative bacteria have an outer membrane, which acts as a protective barrier and excludes many antibiotics. The limited number of antibiotics active against Gram-negative bacteria, along with rising rates of antibiotic resistance, hig…
View article: A Biological Signature for the Inhibition of Outer Membrane Lipoprotein Biogenesis
A Biological Signature for the Inhibition of Outer Membrane Lipoprotein Biogenesis Open
The outer membrane (OM) of Gram-negative bacteria is an essential organelle that acts as a formidable barrier to antibiotics. Increasingly prevalent resistance to existing drugs has exacerbated the need for antibiotic discovery efforts tar…
View article: Mucoidy, a general mechanism for maintaining lytic phage in populations of bacteria
Mucoidy, a general mechanism for maintaining lytic phage in populations of bacteria Open
We present evidence that phage resistance resulting from overproduction of exopolysaccharides, mucoidy, provides a general answer to the longstanding question of how lytic viruses are maintained in populations dominated by bacteria upon wh…
View article: A small-molecule inhibitor of BamA impervious to efflux and the outer membrane permeability barrier
A small-molecule inhibitor of BamA impervious to efflux and the outer membrane permeability barrier Open
Significance The spread of multidrug-resistant infections demands antibiotic development. Although new antibiotics targeting gram-positive bacteria have been developed, it has been more than 50 y since a new class of antibiotics targeting …
View article: Countering Gram-Negative Antibiotic Resistance: Recent Progress in Disrupting the Outer Membrane with Novel Therapeutics
Countering Gram-Negative Antibiotic Resistance: Recent Progress in Disrupting the Outer Membrane with Novel Therapeutics Open
Gram-negative bacteria shield themselves from antibiotics by producing an outer membrane (OM) that forms a formidable permeability barrier. Multidrug resistance among these organisms is a particularly acute problem that is exacerbated by t…
View article: Mucoidy, a general mechanism for maintaining lytic phage in populations of bacteria
Mucoidy, a general mechanism for maintaining lytic phage in populations of bacteria Open
We present evidence that phage resistance resulting from overproduction of exopolysaccharides, mucoidy, provides a general answer to the longstanding question of how lytic viruses are maintained in populations dominated by bacteria upon wh…
View article: A Stress Response Monitoring Lipoprotein Trafficking to the Outer Membrane
A Stress Response Monitoring Lipoprotein Trafficking to the Outer Membrane Open
The outer membrane built by Gram-negative bacteria such as Escherichia coli forms a barrier that prevents antibiotics from entering the cell, limiting clinical options at a time of prevalent antibiotic resistance. Stress responses ensure t…
View article: Lipoproteins and Their Trafficking to the Outer Membrane
Lipoproteins and Their Trafficking to the Outer Membrane Open
Lipoproteins are produced by both Gram-positive and Gram-negative bacteria. Once secreted, lipoproteins are quickly acylated, anchoring them into the plasma membrane. Recent work has shown that Gram-positive bacteria are able to generate c…
View article: Fine-Tuning of σ <sup>E</sup> Activation Suppresses Multiple Assembly-Defective Mutations in Escherichia coli
Fine-Tuning of σ <sup>E</sup> Activation Suppresses Multiple Assembly-Defective Mutations in Escherichia coli Open
Gram-negative bacteria, such as Escherichia coli , inhabit a natural environment that is prone to flux. In order to cope with shifting growth conditions and the changing availability of nutrients, cells must be capable of quickly respondin…
View article: The bacterial outer membrane is an evolving antibiotic barrier
The bacterial outer membrane is an evolving antibiotic barrier Open
The outer membrane (OM) of the diderm “gram-negative” class of bacteria is an essential organelle and a robust permeability barrier that prevents many antibiotics from reaching their intracellular targets (1). The OM is a unique asymmetric…
View article: Inhibitor of intramembrane protease RseP blocks the σ <sup>E</sup> response causing lethal accumulation of unfolded outer membrane proteins
Inhibitor of intramembrane protease RseP blocks the σ <sup>E</sup> response causing lethal accumulation of unfolded outer membrane proteins Open
Significance The σ E stress response monitors outer membrane protein (OMP) assembly. Uninduced, σ E is sequestered to the plasma membrane by its anti-sigma factor, RseA. Mutations perturbing OMP biogenesis induce degradation of RseA by the…
View article: Substrate binding to BamD triggers a conformational change in BamA to control membrane insertion
Substrate binding to BamD triggers a conformational change in BamA to control membrane insertion Open
Significance The outer membrane of Gram-negative bacteria, mitochondria, and chloroplasts contains proteins that adopt β-barrel structures. To maintain the integrity of this structure, the β-barrel assembly machine (Bam) folds and inserts …
View article: Redefining the essential trafficking pathway for outer membrane lipoproteins
Redefining the essential trafficking pathway for outer membrane lipoproteins Open
Significance In Gram-negative bacteria, most lipoproteins synthesized in the inner membrane (IM) are trafficked to the outer membrane (OM). The Lol pathway is the trafficking paradigm: LolCDE releases lipoproteins from the IM; LolA shuttle…
View article: Characterization of a stalled complex on the β-barrel assembly machine
Characterization of a stalled complex on the β-barrel assembly machine Open
Significance The outer membrane of Gram-negative bacteria provides a barrier that allows these organisms to live in a variety of harsh environments. Here, we study the machine responsible for the insertion of integral membrane β-barrel pro…
View article: The CpxQ sRNA Negatively Regulates Skp To Prevent Mistargeting of β-Barrel Outer Membrane Proteins into the Cytoplasmic Membrane
The CpxQ sRNA Negatively Regulates Skp To Prevent Mistargeting of β-Barrel Outer Membrane Proteins into the Cytoplasmic Membrane Open
The promoter most strongly induced upon activation of the Cpx two-component envelope stress response is the cpxP promoter. The 3′ untranscribed region (UTR) of the cpxP transcript is shown to produce a small RNA (sRNA), CpxQ. We investigat…