Explanipedia

Protein succinylome analysis identifies citrate synthase as a central regulator of osteoclast metabolic activity Open

Dayoung Yu, Yue Gao, Marcin Luzarowski, Elisabeth Seebach, Thomas Heitkamp , et al. · 2025

Tumour necrosis factor ligand superfamily member 11 (TNFSF11; RANKL) and macrophage colony‐stimulating factor 1 receptor (M‐CSF) differentiate macrophages into osteoclasts. This process is characterised by changes in metabolic activity tha…

Site-specific activation of the proton pump inhibitor rabeprazole by tetrathiolate zinc centres Open

Teresa Marker, Raphael R. Steimbach, Cecilia Perez-Borrajero, Marcin Luzarowski, Eric Hartmann , et al. · 2025

Proton pump inhibitors have become top-selling drugs worldwide. Serendipitously discovered as prodrugs that are activated by protonation in acidic environments, proton pump inhibitors inhibit stomach acid secretion by covalently modifying …

DNA Binding by the Escherichia coli MinC–MinD Complex Requires the MinC Interdomain Linker Open

Navaneethan Palanisamy, Linda Klauss, Marc Lefèbvre, Jianqin Liang, Götz Hofhaus , et al. · 2025

The Escherichia coli Min system, composed of the proteins MinC, MinD, and MinE, is crucial for directing septum formation at mid-cell. MinD has also been shown to bind DNA in a non-sequence-specific manner and to tether plasmid DNA to lipo…

Mst4, a novel cardiac STRIPAK complex–associated kinase, regulates cardiomyocyte growth and survival and is upregulated in human cardiomyopathy Open

Matthias Eden, Marius Leye, Justus Hahn, Emanuel Heilein, Marcin Luzarowski , et al. · 2024

Myocardial failure is associated with adverse remodeling, including loss of cardiomyocytes, hypertrophy, and alterations in cell-cell contacts. Striatin-interacting phosphatase and kinase (STRIPAK) complexes and their mammalian STE20-like …

The thylakoid proton antiporter KEA3 regulates photosynthesis in response to the chloroplast energy status Open

Michał Uflewski, Tobias Rindfleisch, Kübra Korkmaz, Enrico Tietz, Sarah Mielke , et al. · 2024

Synchronized assembly of the oxidative phosphorylation system controls mitochondrial respiration in yeast Open

Daiana N. Moretti-Horten, Carlotta Peselj, Aslı Aras Taşkin, Lisa Myketin, Uwe Schulte , et al. · 2024

Control of protein stoichiometry is essential for cell function. Mitochondrial oxidative phosphorylation (OXPHOS) presents a complex stoichiometric challenge as the ratio of the electron transport chain (ETC) and ATP synthase must be tight…

Targeted Proteomics Reveals Quantitative Differences in Low-Abundance Glycosyltransferases of Patients with Congenital Disorders of Glycosylation Open

Roman Sakson, Lars Beedgen, Patrick Bernhard, Keziban Merve, Nicole Lübbehusen , et al. · 2024

Protein glycosylation is an essential post-translational modification in all domains of life. Its impairment in humans can result in severe diseases named congenital disorders of glycosylation (CDGs). Most of the glycosyltransferases (GTs)…

Genomic instability in individuals with sex determination defects and germ cell cancer Open

Maria Krivega, J. Zimmer, Anna Slezko, Petra Frank‐Herrmann, Julia Rehnitz , et al. · 2023

The ability to transmit genetic information through generations depends on the preservation of genome integrity. Genetic abnormalities affect cell differentiation, causing tissue specification defects and cancer. We addressed genomic insta…

Protein interactome of 3′,5′‐cAMP reveals its role in regulating the actin cytoskeleton Open

Nicolás E. Figueroa, Peter Franz, Marcin Luzarowski, Federico Martínez-Seidel, Juan C. Moreno , et al. · 2023

SUMMARY Identification of protein interactors is ideally suited for the functional characterization of small molecules. 3′,5′‐cAMP is an evolutionary ancient signaling metabolite largely uncharacterized in plants. To tap into the physiolog…

Hello darkness, my old friend: 3-KETOACYL-COENZYME A SYNTHASE4 is a branch point in the regulation of triacylglycerol synthesis in Arabidopsis thaliana Open

Urszula Luzarowska, Anne-Kathrin Ruß, Jérôme Joubès, Marguerite Batsale, Jędrzej Szymański , et al. · 2023

Plant lipids are important as alternative sources of carbon and energy when sugars or starch are limited. Here, we applied combined heat and darkness or extended darkness to a panel of ∼300 Arabidopsis (Arabidopsis thaliana) accessions to …

3-Mercaptopyruvate sulfur transferase is a protein persulfidase Open

Brandán Pedre, Deepti Talwar, Uladzimir Barayeu, Danny Schilling, Marcin Luzarowski , et al. · 2023

Don't let go: co‐fractionation mass spectrometry for untargeted mapping of protein–metabolite interactomes Open

Dennis Schlossarek, Youjun Zhang, Ewelina Sokołowska, Alisdair R. Fernie, Marcin Luzarowski , et al. · 2022

SUMMARY The chemical complexity of metabolomes goes hand in hand with their functional diversity. Small molecules have many essential roles, many of which are executed by binding and modulating the function of a protein partner. The comple…

The K+exchange antiporter 3 senses the chloroplast energy status to synchronize photosynthesis Open

Michał Uflewski, Tobias Rindfleisch, Kübra Korkmaz, Enrico Tietz, Sarah Mielke , et al. · 2022

Plant photosynthesis contains two functional modules, the light-driven reactions in the thylakoid membrane and the carbon-fixing reactions in the chloroplast stroma. In nature, light availability for photosynthesis often undergoes massive …

Rewiring of the protein–protein–metabolite interactome during the diauxic shift in yeast Open

Dennis Schlossarek, Marcin Luzarowski, Ewelina Sokołowska, Venkatesh P. Thirumalaikumar, Lisa Dengler , et al. · 2022

Genomic instability in patients with sex determination defects and germ cell cancer Open

Maria Krivega, J. Zimmer, Anna Slezko, Petra Frank‐Herrmann, Julia Rehnitz , et al. · 2022

The ability to transmit genetic information through generations depends on preservation of genome integrity. Genetic abnormalities affect cell differentiation, causing tissue specification defects and cancer. We addressed genomic instabili…

A novel role of 3’,5’-cAMP in the regulation of actin cytoskeleton in Arabidopsis Open

Monika Chodasiewicz, Nicolás E. Figueroa, Peter Franz, Marcin Luzarowski, Juan C. Moreno , et al. · 2022

The role of cyclic adenosine monophosphate (3’,5’-cAMP) in plants is not well understood, and here, we report a novel role of 3’,5’-cAMP in regulating the actin cytoskeleton. The 3’,5’-cAMP treatment affects the thermal stability of 51 pro…

Erratum to: Functional characterization of proton antiport regulation in the thylakoid membrane Open

Michał Uflewski, Sarah Mielke, Viviana Correa Galvis, Thekla von Bismarck, Xiaoheng Chen , et al. · 2021

Proteogenic Dipeptides Are Characterized by Diel Fluctuations and Target of Rapamycin Complex-Signaling Dependency in the Model Plant Arabidopsis thaliana Open

Maria Juliana Calderan‐Rodrigues, Marcin Luzarowski, Carolina Cassano Monte‐Bello, Romina I. Minen, Boris M. Zühlke , et al. · 2021

As autotrophic organisms, plants capture light energy to convert carbon dioxide into ATP, nicotinamide adenine dinucleotide phosphate (NADPH), and sugars, which are essential for the biosynthesis of building blocks, storage, and growth. At…

Tyr‐Asp inhibition of glyceraldehyde 3‐phosphate dehydrogenase affects plant redox metabolism Open

Juan C. Moreno, Bruno E. Rojas, Rubén Vicente, Michał Górka, Timon W. Matz , et al. · 2021

How organisms integrate metabolism with the external environment is a central question in biology. Here, we describe a novel regulatory small molecule, a proteogenic dipeptide Tyr-Asp, which improves plant tolerance to oxidative stress by …

Functional characterization of proton antiport regulation in the thylakoid membrane Open

Michał Uflewski, Sarah Mielke, Viviana Correa Galvis, Thekla von Bismarck, Xiaoheng Chen , et al. · 2021

During photosynthesis, energy is transiently stored as an electrochemical proton gradient across the thylakoid membrane. The resulting proton motive force (pmf) is composed of a membrane potential (ΔΨ) and a proton concentration gradient (…

Global mapping of protein–metabolite interactions in Saccharomyces cerevisiae reveals that Ser-Leu dipeptide regulates phosphoglycerate kinase activity Open

Marcin Luzarowski, Rubén Vicente, Andrei Kiselev, Mateusz Wagner, Dennis Schlossarek , et al. · 2021

Protein–metabolite interactions are of crucial importance for all cellular processes but remain understudied. Here, we applied a biochemical approach named PROMIS, to address the complexity of the protein–small molecule interactome in the …

PROMISed: A novel web-based tool to facilitate analysis and visualization of the molecular interaction networks from co-fractionation mass spectrometry (CF-MS) experiments Open

Dennis Schlossarek, Marcin Luzarowski, Ewelina Sokołowska, Michał Górka, Lothar Willmitzer , et al. · 2021

Hello darkness, my old friend: 3-Ketoacyl-Coenzyme A Synthase4 is a branch point in the regulation of triacylglycerol synthesis inArabidopsis thaliana Open

Urszula Luzarowska, Anne-Kathrin Ruß, Jérôme Joubès, Marguerite Batsale, Jędrzej Szymański , et al. · 2020

Due to their sessile lifestyle, plants have evolved unique mechanisms to deal with environmental challenges. Under stress, plant lipids are important as alternative sources of carbon and energy when sugars or starch are limited. Here, we a…

PROMIS: Global Analysis of PROtein‐Metabolite Interactions Open

Ewelina Sokołowska, Dennis Schlossarek, Marcin Luzarowski, Aleksandra Skirycz · 2019

Small molecules are not only intermediates of metabolism, but also play important roles in signaling and in controlling cellular metabolism, growth, and development. Although a few systematic studies have been conducted, the true extent of…

Protein and metabolite composition of Arabidopsis stress granules Open

Monika Kosmacz, Michał Górka, Stephan Schmidt, Marcin Luzarowski, Juan C. Moreno , et al. · 2019

Summary Stress granules ( SG s) are evolutionary conserved aggregates of proteins and untranslated mRNA s formed in response to stress. Despite their importance for stress adaptation, no complete proteome composition has been reported for …

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes Open

Marcin Luzarowski, Izabela Wojciechowska, Aleksandra Skirycz · 2018

Cellular processes are regulated by interactions between biological molecules such as proteins, metabolites, and nucleic acids. While the investigation of protein-protein interactions (PPI) is no novelty, experimental approaches aiming to …

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes Open

Marcin Luzarowski, Izabela Wojciechowska, Aleksandra Skirycz · 2018

Cellular processes are regulated by interactions between biological molecules such as proteins, metabolites, and nucleic acids. While the investigation of protein-protein interactions (PPI) is no novelty, experimental approaches aiming to …

PROMIS, global analysis of PROtein–metabolite interactions using size separation in Arabidopsis thaliana Open

Daniel Veyel, Ewelina Sokołowska, Juan C. Moreno, Sylwia Kierszniowska, Justyna Cichon , et al. · 2018

Interaction of 2',3'-cAMP with Rbp47b plays a role in stress granule formation Open

Monika Kosmacz, Marcin Luzarowski, Olga Kerber, Ewa Leniak, Emilio Gutiérrez-Beltrán , et al. · 2018

29,39-cAMP is an intriguing small molecule that is conserved among different kingdoms. 29,39-cAMP is presumably produced during RNA degradation, with increased cellular levels observed especially under stress conditions. Previously, we obs…

Affinity purification with metabolomic and proteomic analysis unravels diverse roles of nucleoside diphosphate kinases Open

Marcin Luzarowski, Monika Kosmacz, Ewelina Sokołowska, Weronika Jasińska, Lothar Willmitzer , et al. · 2017

Interactions between metabolites and proteins play an integral role in all cellular functions. Here we describe an affinity purification (AP) approach in combination with LC/MS-based metabolomics and proteomics that allows, to our knowledg…

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