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View article: In Vitro Analysis of an Alkalihalobacillus clausii Spore-Based Probiotic Formulation Clarifies the Mechanisms Underlying Its Beneficial Properties
In Vitro Analysis of an Alkalihalobacillus clausii Spore-Based Probiotic Formulation Clarifies the Mechanisms Underlying Its Beneficial Properties Open
Probiotics are microorganisms with recognized beneficial properties that are used to improve host health. In particular, probiotics administered as spores, such as those belonging to the genera Bacillus and Alkalihalobacillus, are attracti…
View article: Cultivating complexity: Advancements in establishing <i>in vitro</i> models for the mucus‐adhering gut microbiota
Cultivating complexity: Advancements in establishing <i>in vitro</i> models for the mucus‐adhering gut microbiota Open
A healthy mucus is essential for maintaining intestinal homeostasis and overall well‐being. In recent years, extensive research focused on understanding the intricate interactions between mucus and the gut microbiota. Mucus‐adhering bacter…
View article: Dissecting the role of the <scp>MS</scp>‐ring protein <scp>FliF</scp> in <i>Bacillus cereus</i> flagella‐related functions
Dissecting the role of the <span>MS</span>‐ring protein <span>FliF</span> in <i>Bacillus cereus</i> flagella‐related functions Open
The flagellar MS‐ring, uniquely constituted by FliF, is essential for flagellar biogenesis and functionality in several bacteria. The aim of this study was to dissect the role of FliF in the Gram‐positive and peritrichously flagellated Bac…
View article: Anti-staphylococcal activity of a polyphenol-rich citrus extract: synergy with β-lactams and low proficiency to induce resistance
Anti-staphylococcal activity of a polyphenol-rich citrus extract: synergy with β-lactams and low proficiency to induce resistance Open
Introduction Antibiotic resistance represents one of the most significant threats to public health in the 21st century. Polyphenols, natural molecules with antibacterial activity produced by plants, are being considered as alternative anti…
View article: In-depth Microbiological Characterization of Urine From Subjects With Type 2 Diabetes
In-depth Microbiological Characterization of Urine From Subjects With Type 2 Diabetes Open
Context Lower urinary tract symptoms (LUTS) are common in type 2 diabetes (T2D), affecting quality of life and potentially leading to medication discontinuation. Among various factors contributing to LUTS, recent observations suggest a cri…
View article: Anti-Staphylococcal Biofilm Effects of a Liposome-Based Formulation Containing Citrus Polyphenols
Anti-Staphylococcal Biofilm Effects of a Liposome-Based Formulation Containing Citrus Polyphenols Open
Biofilms are surface-associated microbial communities embedded in a matrix that is almost impenetrable to antibiotics, thus constituting a critical health threat. Biofilm formation on the cornea or ocular devices can lead to serious and di…
View article: Animal and In Vitro Models as Powerful Tools to Decipher the Effects of Enteric Pathogens on the Human Gut Microbiota
Animal and In Vitro Models as Powerful Tools to Decipher the Effects of Enteric Pathogens on the Human Gut Microbiota Open
Examining the interplay between intestinal pathogens and the gut microbiota is crucial to fully comprehend the pathogenic role of enteropathogens and their broader impact on human health. Valid alternatives to human studies have been intro…
View article: A Millifluidic Chamber for Controlled Shear Stress Testing: Application to Microbial Cultures
A Millifluidic Chamber for Controlled Shear Stress Testing: Application to Microbial Cultures Open
In vitro platforms such as bioreactors and microfluidic devices are commonly designed to engineer tissue models as well as to replicate the crosstalk between cells and microorganisms hosted in the human body. These systems promote nutrient…
View article: Use of S<i>accharomyces boulardii</i> CNCM I‐745 as therapeutic strategy for prevention of nonsteroidal anti‐inflammatory drug‐induced intestinal injury
Use of S<i>accharomyces boulardii</i> CNCM I‐745 as therapeutic strategy for prevention of nonsteroidal anti‐inflammatory drug‐induced intestinal injury Open
Background and Purpose Nonsteroidal anti‐inflammatory drugs (NSAIDs) can be associated with severe adverse digestive effects. This study examined the protective effects of the probiotic Saccharomyces boulardii CNCM I‐745 in a rat model of …
View article: Impact of Bacillus cereus on the Human Gut Microbiota in a 3D In Vitro Model
Impact of Bacillus cereus on the Human Gut Microbiota in a 3D In Vitro Model Open
In vitro models for culturing complex microbial communities are progressively being used to study the effects of different factors on the modeling of in vitro-cultured microorganisms. In previous work, we validated a 3D in vitro model of t…
View article: Development of an <i>In Vitro</i> Model of the Gut Microbiota Enriched in Mucus-Adhering Bacteria
Development of an <i>In Vitro</i> Model of the Gut Microbiota Enriched in Mucus-Adhering Bacteria Open
These findings highlight the importance of mucins in shaping intestinal microbial communities, even those in artificial gut microbiota systems. We propose our in vitro model based on mucin-coated electrospun gelatin structures as a valid d…
View article: Development of an in vitro model of the gut microbiota enriched in mucus-adhering bacteria
Development of an in vitro model of the gut microbiota enriched in mucus-adhering bacteria Open
16S rRNA gene sequencing data in FASTQ format
View article: Development of an in vitro model of the gut microbiota enriched in mucus-adhering bacteria
Development of an in vitro model of the gut microbiota enriched in mucus-adhering bacteria Open
16S rRNA gene sequencing data in FASTQ format
View article: Analysis of the microbial content of probiotic products commercialized worldwide and survivability in conditions mimicking the human gut environment
Analysis of the microbial content of probiotic products commercialized worldwide and survivability in conditions mimicking the human gut environment Open
Introduction Probiotics are living microorganisms that, when administered in adequate amounts, confer a health benefit on the host. Adequate number of living microbes, the presence of specific microorganisms, and their survival in the gast…
View article: Dietary Supplementation with the Probiotic SF68 Reinforces Intestinal Epithelial Barrier in Obese Mice by Improving Butyrate Bioavailability
Dietary Supplementation with the Probiotic SF68 Reinforces Intestinal Epithelial Barrier in Obese Mice by Improving Butyrate Bioavailability Open
Scope Modifications in intestinal microbiota and its metabolites, the short‐chain fatty acids (SCFA) are main factors altering intestinal epithelial barrier integrity and eliciting the onset of a meta‐inflammation observed in obesity. The …
View article: HPLC-MS-MS quantification of short-chain fatty acids actively secreted by probiotic strains
HPLC-MS-MS quantification of short-chain fatty acids actively secreted by probiotic strains Open
Introduction Short-chain fatty acids (SCFAs) are the main by-products of microbial fermentations occurring in the human intestine and are directly involved in the host’s physiological balance. As impaired gut concentrations of acetic, prop…
View article: Designs and methodologies to recreate in vitro human gut microbiota models
Designs and methodologies to recreate in vitro human gut microbiota models Open
The human gut microbiota is widely considered to be a metabolic organ hidden within our bodies, playing a crucial role in the host’s physiology. Several factors affect its composition, so a wide variety of microbes residing in the gut are …
View article: The administration of Enterococcus faecium SF68 counteracts compositional shifts in the gut microbiota of diet-induced obese mice
The administration of Enterococcus faecium SF68 counteracts compositional shifts in the gut microbiota of diet-induced obese mice Open
Microorganisms with probiotic properties are eliciting an increasing interest as coadjuvants in the prevention and treatment of obesity through modulation of the gut microbiota. In this study, a probiotic formulation based on Enterococcus …
View article: In vitro assessment of probiotic attributes for strains contained in commercial formulations
In vitro assessment of probiotic attributes for strains contained in commercial formulations Open
View article: HPLC-MS-MS quantification of short-chain fatty acids secreted by probiotic strains
HPLC-MS-MS quantification of short-chain fatty acids secreted by probiotic strains Open
Short-chain fatty acids (SCFAs) are the main by-products of microbial fermentations occurring in the human intestine and are directly involved in the host’s physiological balance. As impaired gut concentrations of acetic, propionic, and bu…
View article: Characterization of a Bacillus cereus strain associated with a large feed-related outbreak of severe infection in pigs
Characterization of a Bacillus cereus strain associated with a large feed-related outbreak of severe infection in pigs Open
Aims Bacillus cereus is often responsible for foodborne diseases and both local and systemic infections in humans. Cases of infection in other mammals are rather rare. In this study, we report a B. cereus feed-related outbreak that caused …
View article: Study of the Adhesion of the Human Gut Microbiota on Electrospun Structures
Study of the Adhesion of the Human Gut Microbiota on Electrospun Structures Open
Although the adhesion of bacteria on surfaces is a widely studied process, to date, most of the works focus on a single species of microorganisms and are aimed at evaluating the antimicrobial properties of biomaterials. Here, we describe h…
View article: Delivery Mode Shapes the Composition of the Lower Airways Microbiota in Newborns
Delivery Mode Shapes the Composition of the Lower Airways Microbiota in Newborns Open
Radical alterations in the human microbiota composition are well-known to be associated with many pathological conditions. If these aberrations are established at the time of birth, the risk of developing correlated pathologies throughout …
View article: Microbiological Quality and Resistance to an Artificial Gut Environment of Two Probiotic Formulations
Microbiological Quality and Resistance to an Artificial Gut Environment of Two Probiotic Formulations Open
The quality control of probiotic products is the focus of numerous organizations worldwide. Several studies have highlighted the poor microbiological quality of many commercial probiotic formulations in terms of the identity of the contain…
View article: Bone and gut microbiota crosstalk: A novel 3D <em>in vitro</em> approach
Bone and gut microbiota crosstalk: A novel 3D <em>in vitro</em> approach Open
The present research aimed at shedding light on the interplay between the composition of the human gut microbiota and bone cells.
View article: Spotlight on the Compositional Quality of Probiotic Formulations Marketed Worldwide
Spotlight on the Compositional Quality of Probiotic Formulations Marketed Worldwide Open
On the worldwide market, a great number of probiotic formulations are available to consumers as drugs, dietary supplements, and functional foods. For exerting their beneficial effects on host health, these preparations should contain a suf…
View article: <i>In Vitro</i> Resistance and Evolution of Resistance to Tavaborole in Trichophyton rubrum
<i>In Vitro</i> Resistance and Evolution of Resistance to Tavaborole in Trichophyton rubrum Open
Tavaborole is currently used in the topical treatment of onychomycosis. In this study, we analyzed the in vitro emergence/evolution of resistance against tavaborole in Trichophyton rubrum .
View article: A novel 3D in vitro model of the human gut microbiota
A novel 3D in vitro model of the human gut microbiota Open
View article: GTP-Dependent FlhF Homodimer Supports Secretion of a Hemolysin in Bacillus cereus
GTP-Dependent FlhF Homodimer Supports Secretion of a Hemolysin in Bacillus cereus Open
The multidomain (B-NG) protein FlhF, a flagellar biogenesis regulator in several bacteria, is the third paralog of the signal recognition particle (SRP)-GTPases Ffh and FtsY, which are known to drive protein-delivery to the plasma membrane…