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Replace, reduce, and reuse organic solvents in peptide downstream processing: the benefits of dimethyl carbonate over acetonitrile Open
Replacement of acetonitrile with greener alternatives in reversed phase liquid chromatogrpahy for the purification of therapeutic peptides in industrial downstream processing.
A Sustainable Chemo-Enzymatic Approach to the Synthesis of Liraglutide Open
The development of green approaches to peptide synthesis is essential, as peptides are gaining prominence in the pharmaceutical market. Traditional methods often involve harmful solvents and high energy use, prompting a shift toward sustai…
Understanding Glucagon Aggregation: In Silico Insights and Experimental Validation Open
Peptide aggregation poses a significant challenge in biopharmaceutical development and neurodegenerative diseases. This study combines computational simulations and experimental validation to uncover the underlying mechanisms and counterme…
Solid phase peptide synthesis using side-chain unprotected arginine and histidine with Oxyma Pure/TBEC in green solvents Open
Oxyma Pure/TBEC coupling protocol in green solid phase peptide synthesis (GSPPS) eliminates protective groups on Arg and His, enhancing atom economy and reducing impurities during cleavage. The technology was extended to side-chain-free Ty…
Assessing the performance of new chromatographic technologies for the separation of peptide epimeric impurities: the case of Icatibant Open
The biopharmaceutical industry faces the challenge of efficiently characterising impurity profiles of therapeutical peptides, also due to their complex polar and ionisable attributes. This research explores the potential of advanced chroma…
Investigation of the <span>GnRH</span> antagonist degarelix isomerization in biological matrices Open
One of the main objectives of peptide drug design is the improvement of peptide pharmacokinetics with maintaining biological activity, which can be achieved by the complex modifications of the primary structure of the peptides. However, th…
Application of Af4-Multidetection to Liraglutide in Its Formulation: Preserving and Representing Native Aggregation Open
Aggregation is among the most critical parameters affecting the pharmacological and safety profile of peptide Active Pharmaceutical Ingredients (APIs). For this reason, it is of utmost importance to define the exact aggregation state of pe…
Benefits of a Mixed-Mode Stationary Phase to Address the Challenging Purification of an Industrially Relevant Peptide: A Proof-of-Concept Study Open
Peptides are a class of biomolecules with a great potential from the therapeutic point of view, because of their unique biological properties. Industrially, the production stategies adopted produce both the target peptide and a series of i…
Downstream Processing of Therapeutic Peptides by Means of Preparative Liquid Chromatography Open
The market of biomolecules with therapeutic scopes, including peptides, is continuously expanding. The interest towards this class of pharmaceuticals is stimulated by the broad range of bioactivities that peptides can trigger in the human …
View article: Expanding the Use of Dynamic Electrostatic Repulsion Reversed-Phase Chromatography: An Effective Elution Mode for Peptides Control and Analysis
Expanding the Use of Dynamic Electrostatic Repulsion Reversed-Phase Chromatography: An Effective Elution Mode for Peptides Control and Analysis Open
Bioactive peptides are increasingly used in clinical practice. Reversed-phase chromatography using formic or trifluoroacetic acid in the mobile phase is the most widely used technique for their analytical control. However, sometimes it doe…
Replacing piperidine in solid phase peptide synthesis: effective Fmoc removal by alternative bases Open
Solid Phase Peptide Synthesis (SPPS) is a key technology for the production of pharmaceutical grade peptides and the replacement of piperidine with alternative bases is a piece of the puzzle moving toward a greener technology.
Coarse-grained model for the simulation of "active drops" Open
Micro-droplets of fluid can behave in peculiarly “active” ways if subject to the action of external perturbations. The many possible applications and interesting properties they can express experimentally makes them the object of several s…
Modeling Living Cells Response to Surface Tension and Chemical Patterns Open
Mechanobiology is an important epigenetic factor. It influences cell functioning and bears on gene induction, protein synthesis, cell growth, and differentiation. In the presence of patterned chemical cues, living cells can take shapes tha…