Margaret Mungai
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View article: Alterations in Cardiovascular Parameters in 5xFAD Murine Model
Alterations in Cardiovascular Parameters in 5xFAD Murine Model Open
Alzheimer's Disease (AD) is a global health issue, affecting over 6 million people in the United States, with that number expected to increase as the population ages. As a neurodegenerative disorder that affects memory and cognitive functi…
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Featured Cover Open
Cover legend: The cover image is based on the article 3D Mitochondrial Structure in Aging Human Skeletal Muscle: Insights Into MFN‐2‐Mediated Changes by Estevão Scudese et al., https://doi.org/10.1111/acel.70054 [Image: see text]
View article: Mitoepigenetic targeting of age-related dysfunction: mechanisms, therapeutic avenues, and transgenerational implications
Mitoepigenetic targeting of age-related dysfunction: mechanisms, therapeutic avenues, and transgenerational implications Open
Mitochondrial epigenetics, a burgeoning field bridging mitochondrial biology and epigenetic regulation, has emerged as a critical determinant of aging and age-related diseases. While nuclear epigenetics is well-characterized, the mechanism…
View article: 3 <scp>D</scp> Mitochondrial Structure in Aging Human Skeletal Muscle: Insights Into <scp>MFN</scp> ‐2‐Mediated Changes
3 <span>D</span> Mitochondrial Structure in Aging Human Skeletal Muscle: Insights Into <span>MFN</span> ‐2‐Mediated Changes Open
Age‐related skeletal muscle atrophy, known as sarcopenia, is characterized by loss of muscle mass, strength, endurance, and oxidative capacity. Although exercise has been shown to mitigate sarcopenia, the underlying governing mechanisms ar…
View article: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells
Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells Open
Proximity ligation assays (PLAs) use specific antibodies to detect endogenous protein‐protein interactions. PLAs are a highly useful biochemical technique that allow two proteins within proximity to be visualized with fluorescent probes am…
View article: Creating Optimal Western Blot Conditions for OPA1 Isoforms in Skeletal Muscle Cells and Tissue
Creating Optimal Western Blot Conditions for OPA1 Isoforms in Skeletal Muscle Cells and Tissue Open
OPA1 is a dynamin‐related GTPase that modulates mitochondrial dynamics and cristae integrity. Humans carry eight different isoforms of OPA1 and mice carry five, all of which are expressed as short‐ or long‐form isoforms. These isoforms con…
View article: Quantitative assessment of morphological changes in lipid droplets and lipid–mito interactions with aging in brown adipose
Quantitative assessment of morphological changes in lipid droplets and lipid–mito interactions with aging in brown adipose Open
The physical characteristics of brown adipose tissue (BAT) are defined by the presence of multilocular lipid droplets (LDs) within the brown adipocytes and a high abundance of iron‐containing mitochondria, which give it its characteristic …
View article: MICOS Complex Loss Governs Age-Associated Murine Mitochondrial Architecture and Metabolism in the Liver, While Sam50 Dictates Diet Changes
MICOS Complex Loss Governs Age-Associated Murine Mitochondrial Architecture and Metabolism in the Liver, While Sam50 Dictates Diet Changes Open
The liver, the largest internal organ and a metabolic hub, undergoes significant declines due to aging, affecting mitochondrial function and increasing the risk of systemic liver diseases. How the mitochondrial three-dimensional (3D) struc…
View article: Ablation of <i>Sam50</i> is associated with fragmentation and alterations in metabolism in murine and human myotubes
Ablation of <i>Sam50</i> is associated with fragmentation and alterations in metabolism in murine and human myotubes Open
The sorting and assembly machinery (SAM) Complex is responsible for assembling β‐barrel proteins in the mitochondrial membrane. Comprising three subunits, Sam35, Sam37, and Sam50, the SAM complex connects the inner and outer mitochondrial …
View article: A review of undergraduate research programs aimed at underrepresented students
A review of undergraduate research programs aimed at underrepresented students Open
View article: Cover Image, Volume 239, Number 4, April 2024
Cover Image, Volume 239, Number 4, April 2024 Open
Front Cover Caption: The cover image is based on the Research Article ATF4-dependent increase in mitochondrial-endoplasmic reticulum tethering following OPA1 deletion in skeletal muscle by Antentor Hinton Jr. et al., https://doi.org/10.100…
View article: Cover Image, Volume 239, Number 4, April 2024
Cover Image, Volume 239, Number 4, April 2024 Open
Inside Front Cover Caption: The cover image is based on the Research Article ATF4-dependent increase in mitochondrial-endoplasmic reticulum tethering following OPA1 deletion in skeletal muscle by Antentor Hinton Jr. et al., https://doi.org…
View article: Running a successful STEMM summer program: A week‐by‐week guide
Running a successful STEMM summer program: A week‐by‐week guide Open
While some established undergraduate summer programs are effective across many institutions, these programs may only be available to some principal investigators or may not fully address the diverse needs of incoming undergraduates. This a…
View article: ATF4‐dependent increase in mitochondrial‐endoplasmic reticulum tethering following OPA1 deletion in skeletal muscle
ATF4‐dependent increase in mitochondrial‐endoplasmic reticulum tethering following OPA1 deletion in skeletal muscle Open
Mitochondria and endoplasmic reticulum (ER) contact sites (MERCs) are protein‐ and lipid‐enriched hubs that mediate interorganellar communication by contributing to the dynamic transfer of Ca 2+ , lipid, and other metabolites between these…
View article: Defining Mitochondrial Cristae Morphology Changes Induced by Aging in Brown Adipose Tissue (Adv. Biology 1/2024)
Defining Mitochondrial Cristae Morphology Changes Induced by Aging in Brown Adipose Tissue (Adv. Biology 1/2024) Open
Aging The image representing article number 2300186 by Amber Crabtree, Zer Vue, Antentor Hinton Jr., and co-workers depicts 3D reconstruction of folds of the inner mitochondrial membrane, known as cristae. The team elucidate how 3D cristae…
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Cover legend: The cover image is based on the Research Article 3D reconstruction of murine mitochondria reveals changes in structure during aging linked to the MICOS complex by Zer Vue et al., https://doi.org/10.1111/acel.14009 [Image: see…
View article: Human Heart Failure Alters Mitochondria and Fiber 3D Structure Triggering Metabolic Shifts
Human Heart Failure Alters Mitochondria and Fiber 3D Structure Triggering Metabolic Shifts Open
This study, utilizing SBF-SEM, reveals structural alterations in mitochondria and myofibrils in human heart failure (HF). Mitochondria in HF show changes in structure, while myofibrils exhibit increased cross-sectional area and branching. …
View article: 3D Mitochondrial Structure in Aging Human Skeletal Muscle: Insights into MFN-2 Mediated Changes
3D Mitochondrial Structure in Aging Human Skeletal Muscle: Insights into MFN-2 Mediated Changes Open
Age-related atrophy of skeletal muscle, is characterized by loss of mass, strength, endurance, and oxidative capacity during aging. Notably, bioenergetics and protein turnover studies have shown that mitochondria mediate this decline in fu…
View article: <scp>3D</scp> reconstruction of murine mitochondria reveals changes in structure during aging linked to the <scp>MICOS</scp> complex
<span>3D</span> reconstruction of murine mitochondria reveals changes in structure during aging linked to the <span>MICOS</span> complex Open
During aging, muscle gradually undergoes sarcopenia, the loss of function associated with loss of mass, strength, endurance, and oxidative capacity. However, the 3D structural alterations of mitochondria associated with aging in skeletal m…
View article: Protocol for isolating mice skeletal muscle myoblasts and myotubes via differential antibody validation
Protocol for isolating mice skeletal muscle myoblasts and myotubes via differential antibody validation Open
Isolation of skeletal muscles allows for the exploration of many complex diseases. Here, we present a protocol for isolating mice skeletal muscle myoblasts and myotubes that have been differentiated through antibody validation. We describe…
View article: A Comprehensive Approach to Sample Preparation for Electron Microscopy and the Assessment of Mitochondrial Morphology in Tissue and Cultured Cells (Adv. Biology 10/2023)
A Comprehensive Approach to Sample Preparation for Electron Microscopy and the Assessment of Mitochondrial Morphology in Tissue and Cultured Cells (Adv. Biology 10/2023) Open
Mitochondrial Morphology In article number 2200202 Antentor Hinton Jr., Brian Glancy, Eugenia Trushina, E. Dale Abel and co-workers present a 3D reconstruction of mitochondria (red), actin (blue), lysosome (pink), lipid droplet (teal), mit…
View article: Quantitative Assessment of Morphological Changes in Lipid Droplets and Lipid-Mito Interactions with Aging in Brown Adipose
Quantitative Assessment of Morphological Changes in Lipid Droplets and Lipid-Mito Interactions with Aging in Brown Adipose Open
The physical characteristics of brown adipose tissue (BAT) are defined by the presence of multilocular lipid droplets (LD) within the brown adipocytes and a high abundance of iron-containing mitochondria, which give it its characteristic c…
View article: Defining Mitochondrial Cristae Morphology Changes Induced by Aging in Brown Adipose Tissue
Defining Mitochondrial Cristae Morphology Changes Induced by Aging in Brown Adipose Tissue Open
Mitochondria are required for energy production and even give brown adipose tissue (BAT) its characteristic color due to their high iron content and abundance. The physiological function and bioenergetic capacity of mitochondria are connec…
View article: Systematic Transmission Electron Microscopy‐Based Identification and 3D Reconstruction of Cellular Degradation Machinery (Adv. Biology 6/2023)
Systematic Transmission Electron Microscopy‐Based Identification and 3D Reconstruction of Cellular Degradation Machinery (Adv. Biology 6/2023) Open
View article: In the Age of Machine Learning Cryo‐EM Research is Still Necessary: A Path toward Precision Medicine
In the Age of Machine Learning Cryo‐EM Research is Still Necessary: A Path toward Precision Medicine Open
Machine learning has proven useful in analyzing complex biological data and has greatly influenced the course of research in structural biology and precision medicine. Deep neural network models oftentimes fail to predict the structure of …
View article: Creating Optimal Conditions for OPA1 Isoforms by Western Blot in Skeletal Muscle Cells and Tissue
Creating Optimal Conditions for OPA1 Isoforms by Western Blot in Skeletal Muscle Cells and Tissue Open
Summary OPA1 is a dynamin-related GTPase that modulates various mitochondrial functions and is involved in mitochondrial morphology. There are eight different isoforms of OPA1 in humans and five different isoforms in mice that are expresse…
View article: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells
Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells Open
Proximity ligation assays (PLA) use specific antibodies to detect endogenous protein-protein interactions. PLA is a highly useful biochemical technique that allows two proteins within close proximity to be visualized with fluorescent probe…
View article: Ablation of<i>Sam50</i>is associated with fragmentation and alterations in metabolism in murine and human myotubes
Ablation of<i>Sam50</i>is associated with fragmentation and alterations in metabolism in murine and human myotubes Open
The Sorting and Assembly Machinery (SAM) Complex is responsible for assembling β-barrel proteins in the mitochondrial membrane. Comprising three subunits, Sam35, Sam37, and Sam50, the SAM complex connects the inner and outer mitochondrial …
View article: Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation
Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation Open
Summary Isolation of skeletal muscles allows for the exploration of many complex diseases. Fibroblasts and myoblast play important roles in skeletal muscle morphology and function. However, skeletal muscles are complex and made up of many …
View article: Defining Mitochondrial Cristae Morphology Changes Induced by Aging in Brown Adipose Tissue
Defining Mitochondrial Cristae Morphology Changes Induced by Aging in Brown Adipose Tissue Open
Mitochondria are required for energy production and even give brown adipose tissue (BAT) its characteristic color due to their high iron content and abundance. The physiological function and bioenergetic capacity of mitochondria are connec…