Margot Riggi
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View article: ExoSloNano: multimodal nanogold labels for identification of macromolecules in live cells and cryo-electron tomograms
ExoSloNano: multimodal nanogold labels for identification of macromolecules in live cells and cryo-electron tomograms Open
In situ cryo-electron microscopy (cryo-EM) enables the direct interrogation of structure–function relationships by resolving macromolecular structures in their native cellular environment. Recent progress in sample preparation, imaging and…
View article: A multiscale imaging approach to studying the harpoon-like invasion organelle from microsporidian pathogens
A multiscale imaging approach to studying the harpoon-like invasion organelle from microsporidian pathogens Open
Microsporidia are tiny, single-celled parasites similar to fungi that infect a wide range of animal species, from worms and honey bees to humans. In humans, these opportunistic pathogens can cause life-threatening infections in immunocompr…
View article: Making plant tissue accessible for cryo-electron tomography
Making plant tissue accessible for cryo-electron tomography Open
Cryo-Electron Tomography (cryo-ET) allows to visualize the molecular architecture of pristinely preserved cells and tissues. The workflow of sample preparation for cryo-ET is rather complex; it involves vitrification by rapid freezing foll…
View article: Making plant tissue accessible for cryo-electron tomography
Making plant tissue accessible for cryo-electron tomography Open
Cryo-Electron Tomography (cryo-ET) allows to visualize the molecular architecture of pristinely preserved cells and tissues. The workflow of sample preparation for cryo-ET is rather complex; it involves vitrification by rapid freezing foll…
View article: Quantitative spatial analysis of chromatin biomolecular condensates using cryoelectron tomography
Quantitative spatial analysis of chromatin biomolecular condensates using cryoelectron tomography Open
Phase separation is an important mechanism to generate certain biomolecular condensates and organize the cell interior. Condensate formation and function remain incompletely understood due to difficulties in visualizing the condensate inte…
View article: Cryo-ET reveals the in situ architecture of the polar tube invasion apparatus from microsporidian parasites
Cryo-ET reveals the in situ architecture of the polar tube invasion apparatus from microsporidian parasites Open
Microsporidia are divergent fungal pathogens that employ a unique harpoon-like apparatus called the polar tube (PT) to invade host cells. The long PT is fired out of the microsporidian spore over the course of just a few hundred millisecon…
View article: Making plant tissue accessible for cryo-electron tomography
Making plant tissue accessible for cryo-electron tomography Open
Cryo-Electron Tomography (cryo-ET) allows to visualize the molecular architecture of pristinely preserved cells and tissues. The workflow of sample preparation for cryo-ET is rather complex; it involves vitrification by rapid freezing foll…
View article: Multi-scale structure of chromatin condensates rationalizes phase separation and material properties
Multi-scale structure of chromatin condensates rationalizes phase separation and material properties Open
Biomolecular condensates, compartments that concentrate molecules without surrounding membranes, are integral to numerous cellular processes. The structure and interaction networks of molecules within condensates remain poorly understood. …
View article: Quantitative Spatial Analysis of Chromatin Biomolecular Condensates using Cryo-Electron Tomography
Quantitative Spatial Analysis of Chromatin Biomolecular Condensates using Cryo-Electron Tomography Open
Phase separation is an important mechanism to generate certain biomolecular condensates and organize the cell interior. Condensate formation and function remain incompletely understood due to difficulties in visualizing the condensate inte…
View article: ExoSloNano: Multi-Modal Nanogold Tags for identification of Macromolecules in Live Cells & Cryo-Electron Tomograms
ExoSloNano: Multi-Modal Nanogold Tags for identification of Macromolecules in Live Cells & Cryo-Electron Tomograms Open
In situ cryo-Electron Microscopy (cryo-EM) enables the direct interrogation of structure-function relationships by resolving macromolecular structures in their native cellular environment. Tremendous progress in sample preparation, imaging…
View article: Cryo-ET reveals the<i>in situ</i>architecture of the polar tube invasion apparatus from microsporidian parasites
Cryo-ET reveals the<i>in situ</i>architecture of the polar tube invasion apparatus from microsporidian parasites Open
Microsporidia are divergent fungal pathogens that employ a harpoon-like apparatus called the polar tube (PT) to invade host cells. The PT architecture and its association with neighboring organelles remain poorly understood. Here, we use c…
View article: Depicting a cellular space occupied by condensates
Depicting a cellular space occupied by condensates Open
Condensates have emerged as a new way to understand how cells are organized, and have been invoked to play crucial roles in essentially all cellular processes. In this view, the cell is occupied by numerous assemblies, each composed of mem…
View article: An educational visual resource to support understanding of liquid–liquid phase separation
An educational visual resource to support understanding of liquid–liquid phase separation Open
In collaboration with educators and researchers, we created an online resource called Phase Separation 101 to help undergraduate students understand the basics of liquid–liquid phase separation, an emerging and complex concept in cell biol…
View article: A new tool for annotating scientific animations and supporting scientific dialogue
A new tool for annotating scientific animations and supporting scientific dialogue Open
A new interactive annotation interface supports a detailed molecular animation of the SARS-CoV-2 life cycle. With this tool, users can interactively explore the data used to create the animation and engage in scientific discourse through c…
View article: Tiny molecular probes reveal invisible forces inside cells
Tiny molecular probes reveal invisible forces inside cells Open
Tiny fluorescent molecules, inspired by the color-changing lobster pigment, have been developed to visualize and measure the tension of various cellular membranes. It is the first time scientists can observe the consequences of an invisibl…
View article: Flipper Probes for the Community
Flipper Probes for the Community Open
This article describes four fluorescent membrane tension probes that have been designed, synthesized, evaluated, commercialized and applied to current biology challenges in the context of the NCCR Chemical Biology. Their names are Flipper-…
View article: The flipside of the TOR coin – TORC2 and plasma membrane homeostasis at a glance
The flipside of the TOR coin – TORC2 and plasma membrane homeostasis at a glance Open
Target of rapamycin (TOR) is a serine/threonine protein kinase conserved in most eukaryote organisms. TOR assembles into two multiprotein complexes (TORC1 and TORC2), which function as regulators of cellular growth and homeostasis by servi…
View article: TORC2 controls endocytosis through plasma membrane tension
TORC2 controls endocytosis through plasma membrane tension Open
Target of rapamycin complex 2 (TORC2) is a conserved protein kinase that regulates multiple plasma membrane (PM)–related processes, including endocytosis. Direct, chemical inhibition of TORC2 arrests endocytosis but with kinetics that is r…
View article: TORC2 Affects Endocytosis through Plasma Membrane Tension
TORC2 Affects Endocytosis through Plasma Membrane Tension Open
Target Of Rapamycin complex 2 (TORC2) is a conserved protein kinase that regulates multiple plasma membrane (PM)-related processes including endocytosis. Direct, chemical inhibition of TORC2 arrests endocytosis but with kinetics that are r…
View article: Data from: Systematic analysis of complex genetic interactions
Data from: Systematic analysis of complex genetic interactions Open
To systematically explore complex genetic interactions, we constructed ~200,000 yeast triple mutants and scored negative trigenic interactions. We selected double-mutant query genes across a broad spectrum of biological processes, spanning…