Maria L. Sapar
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View article: A repository of Ogden syndrome (NAA10) and NAA15 patient derived iPSC lines and isogenic pairs by X-chromosome screening and genome-editing.
A repository of Ogden syndrome (NAA10) and NAA15 patient derived iPSC lines and isogenic pairs by X-chromosome screening and genome-editing. Open
Amino-terminal (Nt-) acetylation (NTA) is a common protein modification, affecting 80% of cytosolic proteins in humans. The human essential gene, NAA10, encodes the enzyme NAA10, as the catalytic subunit for the N-terminal acetyltransferas…
View article: Patient iPSC models reveal glia-intrinsic phenotypes in multiple sclerosis
Patient iPSC models reveal glia-intrinsic phenotypes in multiple sclerosis Open
Summary Multiple sclerosis (MS) is considered an inflammatory and neurodegenerative disease of the central nervous system, typically resulting in significant neurological disability that worsens over time. While considerable progress has b…
View article: The <i>Drosophila</i> chemokine–like Orion bridges phosphatidylserine and Draper in phagocytosis of neurons
The <i>Drosophila</i> chemokine–like Orion bridges phosphatidylserine and Draper in phagocytosis of neurons Open
Phagocytic clearance of degenerating neurons is triggered by “eat-me” signals exposed on the neuronal surface. The conserved neuronal eat-me signal phosphatidylserine (PS) and the engulfment receptor Draper (Drpr) mediate phagocytosis of d…
View article: Proteomic Alterations and Novel Markers of Neurotoxic Reactive Astrocytes in Human Induced Pluripotent Stem Cell Models
Proteomic Alterations and Novel Markers of Neurotoxic Reactive Astrocytes in Human Induced Pluripotent Stem Cell Models Open
Astrocytes respond to injury, infection, and inflammation in the central nervous system by acquiring reactive states in which they may become dysfunctional and contribute to disease pathology. A sub-state of reactive astrocytes induced by …
View article: The<i>Drosophila</i>chemokine-like Orion bridges phosphatidylserine and Draper in phagocytosis of neurons
The<i>Drosophila</i>chemokine-like Orion bridges phosphatidylserine and Draper in phagocytosis of neurons Open
Phagocytic clearance of degenerating neurons is triggered by “eat-me” signals exposed on the neuronal surface. The conserved neuronal eat-me signal phosphatidylserine (PS) and the engulfment receptor Draper (Drpr) mediate phagocytosis of d…
View article: Phagocytosis and self-destruction break down dendrites of <i>Drosophila</i> sensory neurons at distinct steps of Wallerian degeneration
Phagocytosis and self-destruction break down dendrites of <i>Drosophila</i> sensory neurons at distinct steps of Wallerian degeneration Open
Significance Mutations in the nicotinamide adenine dinucleotide (NAD + ) biosynthesis pathway are associated with progressive neurodegeneration; neuronal injury causes rapid breakdown of damaged axons and dendrites. NAD + reduction is thou…
View article: Phagocytosis and self-destruction execute dendrite degeneration of Drosophila sensory neurons at distinct levels of NAD+ reduction
Phagocytosis and self-destruction execute dendrite degeneration of Drosophila sensory neurons at distinct levels of NAD+ reduction Open
After injury, severed dendrites and axons expose the "eat-me" signal phosphatidylserine (PS) on their surface and degenerate by disassembly. While axon degeneration is controlled by a conserved "axon-death" pathway that is thought to activ…
View article: Die in pieces: How Drosophila sheds light on neurite degeneration and clearance
Die in pieces: How Drosophila sheds light on neurite degeneration and clearance Open
View article: Robust CRISPR/Cas9-Mediated Tissue-Specific Mutagenesis Reveals Gene Redundancy and Perdurance in<i>Drosophila</i>
Robust CRISPR/Cas9-Mediated Tissue-Specific Mutagenesis Reveals Gene Redundancy and Perdurance in<i>Drosophila</i> Open
The CRISPR/Cas9 gene editing system continues to push the boundaries of genetic analysis. Here, papers from Farboud, Severson, and Meyer and Poe et al. describe cutting-edge advances for CRISPR use. Farboud, Severson, and Meyer.... Tissue-…
View article: Phosphatidylserine Externalization Results from and Causes Neurite Degeneration in Drosophila
Phosphatidylserine Externalization Results from and Causes Neurite Degeneration in Drosophila Open
Phagocytic clearance of degenerating dendrites or axons is critical for maintaining tissue homeostasis and preventing neuroinflammation. Externalized phosphatidylserine (PS) has been postulated to be an "eat-me" signal allowing recognition…
View article: Highly efficient CRISPR/Cas9-mediated tissue specific mutagenesis in<i>Drosophila</i>
Highly efficient CRISPR/Cas9-mediated tissue specific mutagenesis in<i>Drosophila</i> Open
Tissue-specific loss-of-function (LOF) analysis is an essential approach for characterizing gene function. Here we describe an efficient CRISPR -mediated t issue- r estricted m utagenesis (CRISPR-TRiM) method for ablating gene function in …
View article: HIV Glycoprotein Gp120 Impairs Fast Axonal Transport by Activating Tak1 Signaling Pathways
HIV Glycoprotein Gp120 Impairs Fast Axonal Transport by Activating Tak1 Signaling Pathways Open
Sensory neuropathies are the most common neurological complication of HIV. Of these, distal sensory polyneuropathy (DSP) is directly caused by HIV infection and characterized by length-dependent axonal degeneration of dorsal root ganglion …
View article: The transcriptional repressor Sum1p counteracts Sir2p in regulation of the actin cytoskeleton, mitochondrial quality control and replicative lifespan in Saccharomyces cerevisiae
The transcriptional repressor Sum1p counteracts Sir2p in regulation of the actin cytoskeleton, mitochondrial quality control and replicative lifespan in Saccharomyces cerevisiae Open
Increasing the stability or dynamics of the actin cytoskeleton can extend lifespan in C. elegans and S. cerevisiae. Actin cables of budding yeast, bundles of actin filaments that mediate cargo transport, affect lifespan contr…