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View article: Transcriptomic Identification of Immune-Related Hubs as Candidate Predictor Biomarkers of Therapeutic Response in Psoriasis
Transcriptomic Identification of Immune-Related Hubs as Candidate Predictor Biomarkers of Therapeutic Response in Psoriasis Open
Psoriasis is a chronic inflammatory skin disease driven by genetic, environmental, and immune factors. While biologics like adalimumab (anti-TNFα) and risankizumab (anti-IL-23) have improved outcomes, patient response variability remains u…
View article: Regulatory challenges and risk assessment of graphene-enabled products: insights for safe commercialisation in Europe
Regulatory challenges and risk assessment of graphene-enabled products: insights for safe commercialisation in Europe Open
The development of graphene-enabled products in a variety of industrial sectors like medical devices, textiles, aerospace, or food contact materials has raised significant regulatory challenges with regards to risk assessment. The work per…
View article: Tailoring DNA Surface Interactions on Single-Layer Graphene: Comparative Analysis of Pyrene, Acridine, and Fluorenyl Methyl Linkers
Tailoring DNA Surface Interactions on Single-Layer Graphene: Comparative Analysis of Pyrene, Acridine, and Fluorenyl Methyl Linkers Open
This study investigates the effect of different linkers and solvents on the immobilization of DNA probes on graphene surfaces, which are crucial for developing high-performance biosensors. Quartz crystal microbalance with dissipation (QCM-…
View article: Surface monitoring of L. monocytogenes by real-time fluorescence and colorimetric LAMP
Surface monitoring of L. monocytogenes by real-time fluorescence and colorimetric LAMP Open
Listeria monocytogenes is a major foodborne pathogen affecting developing, and developed countries. The analysis of food contact surfaces in food industries is key for better controlling this pathogen. The current study focused on the deve…
View article: Sesame Detection in Food Using DNA-Functionalized Gold Nanoparticles: A Sensitive, Rapid, and Cost-Effective Colorimetric Approach
Sesame Detection in Food Using DNA-Functionalized Gold Nanoparticles: A Sensitive, Rapid, and Cost-Effective Colorimetric Approach Open
Food safety control is a key issue in the food and agriculture industries. For such purposes, developing miniaturized analytical methods is critical for enabling the rapid and sensitive detection of food supplements, allergens, and polluta…
View article: Microfluidic paper-based analytical aptasensor for fluorometric β-lactoglobulin determination
Microfluidic paper-based analytical aptasensor for fluorometric β-lactoglobulin determination Open
Herein, we present a new luminescent-based analytical method for sensitive fluorometric determination of a milk allergen, β-lactoglobulin (β-LG), in food products based on a portable, low-cost, and easy-to-use microfluidic paper-based anal…
View article: Comparative performance of two different detection chemistries of qPCR for their implementation in a same-day detection method to determine the presence of Shiga toxin–producing Escherichia coli in ready-to-eat salads
Comparative performance of two different detection chemistries of qPCR for their implementation in a same-day detection method to determine the presence of Shiga toxin–producing Escherichia coli in ready-to-eat salads Open
Shiga toxin-producing E. coli (STEC), are among the most frequently reported foodborne pathogens worldwide. They produce two powerful cytotoxins, called Shiga toxins (Stx1 and Stx2). Most DNA-based methods target these genes, and rely on l…
View article: Evaluation of the Novel mTA10 Selective Broth, MSB, for the Co-Enrichment and Detection of Salmonella spp., Escherichia coli O157 and Listeria monocytogenes in Ready-to-Eat Salad Samples
Evaluation of the Novel mTA10 Selective Broth, MSB, for the Co-Enrichment and Detection of Salmonella spp., Escherichia coli O157 and Listeria monocytogenes in Ready-to-Eat Salad Samples Open
Multiplex assays implementing DNA-based methods have been demonstrated as suitable alternatives to culture-based microbiological methods; however, in most cases, they still require a suitable enrichment step. Finding suitable enrichment co…
View article: Assessment of the presence of Acinetobacter spp. resistant to β-lactams in commercial ready-to-eat salad samples
Assessment of the presence of Acinetobacter spp. resistant to β-lactams in commercial ready-to-eat salad samples Open
Acinetobacter baumannii is a well-known nosocomial infection causing agent. However, other Acinetobacter spp. have also been implicated in cases of human infection. Additionally, these bacteria are known for the development of antibiotic r…
View article: Evaluation of the Antimicrobial Activity of Chitosan Nanoparticles against Listeria monocytogenes
Evaluation of the Antimicrobial Activity of Chitosan Nanoparticles against Listeria monocytogenes Open
Chitosan is obtained from the deacetylation of chitin, and it is known to possess antimicrobial activity. It has attracted attention as it may be used for treating infections caused by different types of microorganisms due to its broad spe…
View article: Miniaturized Microscale Solid-Phase Extraction-Based Module for Highly Efficient DNA Extraction and Purification from Grapevine Samples
Miniaturized Microscale Solid-Phase Extraction-Based Module for Highly Efficient DNA Extraction and Purification from Grapevine Samples Open
Several developments over the last few years are being directed toward improving DNA-based analysis to simplify, miniaturize, and reduce the time and cost of analysis, with the objective to allow its use in decentralized settings. One of t…
View article: Phage amplification coupled with loop-mediated isothermal amplification (PA-LAMP) for same-day detection of viable Salmonella Enteritidis in raw poultry meat
Phage amplification coupled with loop-mediated isothermal amplification (PA-LAMP) for same-day detection of viable Salmonella Enteritidis in raw poultry meat Open
Salmonella Enteritidis is the main serotype responsible for human salmonellosis in the European Union. One of the main sources of Salmonella spp. in the food chain are poultry products, such as eggs or chicken meat. In recent years, molecu…
View article: Evaluation of Covalent Organic Frameworks for the low-cost, rapid detection of Shiga Toxin-producing Escherichia coli in ready-to-eat salads
Evaluation of Covalent Organic Frameworks for the low-cost, rapid detection of Shiga Toxin-producing Escherichia coli in ready-to-eat salads Open
It was demonstrated for the first time that COFs are suitable for DNA extraction and purification. In addition to this, due to the tunable nature of these materials, it is envisioned that future modifications in terms of pore size or combi…
View article: Evaluation of the effect of outer primer structure, and inner primer linker sequences, in the performance of Loop-mediated isothermal amplification
Evaluation of the effect of outer primer structure, and inner primer linker sequences, in the performance of Loop-mediated isothermal amplification Open
Loop-mediated isothermal amplification, or LAMP, is nowadays the most popular isothermal nucleic acid amplification technique. This technique implements a minimum of four primers, named outer (F3/B3) and inner primers (FIP/BIP). The inner …
View article: Integrated Approach from Sample-to-Answer for Grapevine Varietal Identification on a Portable Graphene Sensor Chip
Integrated Approach from Sample-to-Answer for Grapevine Varietal Identification on a Portable Graphene Sensor Chip Open
Identifying grape varieties in wine, related products, and raw materials is of great interest for enology and to ensure its authenticity. However, these matrices' complexity and low DNA content make this analysis particularly challenging. …
View article: Dual-Mode Gold Nanoparticle-Based Method for Early Detection of Acanthamoeba
Dual-Mode Gold Nanoparticle-Based Method for Early Detection of Acanthamoeba Open
Acanthamoeba keratitis is an aggressive and rapidly progressing ocular pathology whose main risk factor is the use of contact lenses. An early and differential diagnosis is considered the main factor to prevent the progression and improve …
View article: Towards on-site detection of gluten-containing cereals with a portable and miniaturized prototype combining isothermal DNA amplification and naked eye detection
Towards on-site detection of gluten-containing cereals with a portable and miniaturized prototype combining isothermal DNA amplification and naked eye detection Open
Miniaturization enables the development of portable devices for rapid on-site DNA analysis, providing cheaper and more convenient systems for target DNA detection in resource-limited settings. Miniaturized devices for DNA-based analysis ar…
View article: Application of MinION sequencing as a tool for the rapid detection and characterization of Listeria monocytogenes in smoked salmon
Application of MinION sequencing as a tool for the rapid detection and characterization of Listeria monocytogenes in smoked salmon Open
Microbial pathogens may be present in different types of foods, and hence the development of novel methods to assure consumers' safeness is of great interest. Molecular methods are known to provide sensitive and rapid results; however, the…
View article: Programmable graphene-based microfluidic sensor for DNA detection
Programmable graphene-based microfluidic sensor for DNA detection Open
This study presents the development of a lab-on-a-chip (LoC) by integrating a graphene field-effect transistor (FET) chip with a programmable microfluidic device for DNA detection. The real-time biochemical events on the graphene FET chip …
View article: The Protein‐Templated Synthesis of Enzyme‐Generated Aptamers
The Protein‐Templated Synthesis of Enzyme‐Generated Aptamers Open
Inspired by the chemical synthesis of molecularly imprinted polymers, we demonstrated for the first time, the protein‐target mediated synthesis of enzyme‐generated aptamers (EGAs). We prepared pre‐polymerisation mixtures containing differe…
View article: The Protein‐Templated Synthesis of Enzyme‐Generated Aptamers
The Protein‐Templated Synthesis of Enzyme‐Generated Aptamers Open
Inspired by the chemical synthesis of molecularly imprinted polymers, we demonstrated for the first time, the protein‐target mediated synthesis of enzyme‐generated aptamers (EGAs). We prepared pre‐polymerisation mixtures containing differe…
View article: Short pre-enrichment and modified matrix lysis. A comparative study towards same-day detection of Listeria monocytogenes
Short pre-enrichment and modified matrix lysis. A comparative study towards same-day detection of Listeria monocytogenes Open
Listeria monocytogenes is a foodborne pathogen of particular concern in ready-to-eat foods. Different methodologies have been published in recent years, in order to reduce the time of analysis of this pathogen but, best case scenario, all …
View article: Microsatellite Markers in Olives (Olea europaea L.): Utility in the Cataloging of Germplasm, Food Authenticity and Traceability Studies
Microsatellite Markers in Olives (Olea europaea L.): Utility in the Cataloging of Germplasm, Food Authenticity and Traceability Studies Open
The olive fruit, a symbol of Mediterranean diets, is a rich source of antioxidants and oleic acid (55–83%). Olive genetic resources, including cultivated olives (cultivars), wild olives as well as related subspecies, are distributed widely…
View article: Evaluation of simple sequence repeats (SSR) and single nucleotide polymorphism (SNP)-based methods in olive varieties from the Northwest of Spain and potential for miniaturization
Evaluation of simple sequence repeats (SSR) and single nucleotide polymorphism (SNP)-based methods in olive varieties from the Northwest of Spain and potential for miniaturization Open
Miniaturization of DNA-based techniques can bring interesting advantages for food analysis, such as portability of complex analytical procedures. In the olive oil industry, miniaturization can be particularly interesting for authenticity a…