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View article: Artificial Intelligence in Thyroid Cytopathology: Diagnostic and Technical Insights
Artificial Intelligence in Thyroid Cytopathology: Diagnostic and Technical Insights Open
Fine-needle aspiration cytology (FNAC) is the cornerstone of thyroid nodule evaluation, standardized by the Bethesda System. However, indeterminate categories (Bethesda III–IV) remain a major challenge, often leading to unnecessary surgery…
View article: Harmonization trial on ESR1 testing strategies in ER+/HER2- breast cancer patients: an Italian experience
Harmonization trial on ESR1 testing strategies in ER+/HER2- breast cancer patients: an Italian experience Open
View article: Did selective kinase inhibitors change the management of patients with radioiodine-refractory thyroid cancer?
Did selective kinase inhibitors change the management of patients with radioiodine-refractory thyroid cancer? Open
Objective To analyse at our institution the criteria for selecting a first-line therapy for patients with advanced radioiodine-refractory thyroid cancer and their clinical responses, safety and survival outcomes. Patients and methods We ex…
View article: RNA-Based Next-Generation Sequencing in Non-Small Cell Lung Cancer patients: data from Campania, Italy
RNA-Based Next-Generation Sequencing in Non-Small Cell Lung Cancer patients: data from Campania, Italy Open
In this study, we provide real-world experience on RNA-based NGS analysis in patients with advanced stage NSCLC.
View article: How Molecular and Ancillary Tests Can Help in Challenging Cytopathology Cases: Insights from the International Molecular Cytopathology Meeting
How Molecular and Ancillary Tests Can Help in Challenging Cytopathology Cases: Insights from the International Molecular Cytopathology Meeting Open
Over the past decade, molecular cytopathology has emerged as a relevant area of modern pathology. Notably, in patients with advanced-stage cancer, cytological samples could be the only material available for diagnosis and molecular biomark…
View article: Non-Small Cell Lung Cancer Testing on Reference Specimens: An Italian Multicenter Experience
Non-Small Cell Lung Cancer Testing on Reference Specimens: An Italian Multicenter Experience Open
View article: Liquid biopsy for lung cancer: A cross section on the diagnostic routine experience of a referral Italian institution
Liquid biopsy for lung cancer: A cross section on the diagnostic routine experience of a referral Italian institution Open
This review highlights the technical suitability of an NGS-based liquid biopsy system for the analysis of clinically relevant mutations in NSCLC patients.
View article: Technical Validation of a Fully Integrated NGS Platform in the Real-World Practice of Italian Referral Institutions
Technical Validation of a Fully Integrated NGS Platform in the Real-World Practice of Italian Referral Institutions Open
Aims: To date, precision medicine has played a pivotal role in the clinical administration of solid-tumor patients. In this scenario, a rapidly increasing number of predictive biomarkers have been approved in diagnostic practice or are cur…
View article: Technical validation of a fully integrated NGS platform in real-world practice of Italian referral institution
Technical validation of a fully integrated NGS platform in real-world practice of Italian referral institution Open
Aims: To date, precision medicine plays a pivotal role in the clinical administration of solid tumor patients. In this scenario, a rapidly increasing number of predictive biomarkers have been approved in diagnostic practice or are currentl…
View article: Evaluating local thyroid cytopathology practices by molecular quality metrics: A multi‐institutional study on 4651 FNAs with a focus on the role of the interventional cytopathologist
Evaluating local thyroid cytopathology practices by molecular quality metrics: A multi‐institutional study on 4651 FNAs with a focus on the role of the interventional cytopathologist Open
Background The diagnostic accuracy of thyroid fine‐needle aspiration (FNA) can be highly influenced by the technical skills of the operator performing the procedure and by interobserver variability in microscopic interpretation. This is pa…
View article: Multiple predictive biomarker testing in melanoma: Another challenge in identifying the optimal approach on cytological samples
Multiple predictive biomarker testing in melanoma: Another challenge in identifying the optimal approach on cytological samples Open
Background The management of cutaneous melanoma has changed dramatically in recent years thanks to the development of tyrosine kinase and immune‐checkpoint inhibitors (ICIs). Thus, multiple biomarker testing is becoming ever more important…
View article: Evaluation of the Molecular Landscape in PD-L1 Positive Metastatic NSCLC: Data from Campania, Italy
Evaluation of the Molecular Landscape in PD-L1 Positive Metastatic NSCLC: Data from Campania, Italy Open
Background: Immune-checkpoint inhibitors (ICIs) have increased and improved the treatment options for patients with non-oncogene-addicted advanced stage non-small cell lung cancer (NSCLC). However, the role of ICIs in oncogene-addicted adv…
View article: Fine-Needle Aspiration Is Suitable for Breast Cancer BRCA Molecular Assessment: A Case Report
Fine-Needle Aspiration Is Suitable for Breast Cancer BRCA Molecular Assessment: A Case Report Open
Breast cancer is the most common cause of cancer-related deaths in the female population worldwide. To the best of our knowledge, breast cancer (BRCA)1/2 gene mutations have not been described yet on breast cancer cytological specimens. He…
View article: BRCA1/2 NGS Somatic Testing in Clinical Practice: A Short Report
BRCA1/2 NGS Somatic Testing in Clinical Practice: A Short Report Open
High-grade serous ovarian carcinoma (HGSOC) is the most common subtype of all ovarian carcinomas. HGSOC harboring BRCA1/2 germline or somatic mutations are sensitive to the poly (adenosine diphosphate-ribose) polymerase inhibitors (PARPi).…
View article: Methods for Actionable Gene Fusion Detection in Lung Cancer: Now and in the Future
Methods for Actionable Gene Fusion Detection in Lung Cancer: Now and in the Future Open
Although gene fusions occur rarely in non-small-cell lung cancer (NSCLC) patients, they represent a relevant target in treatment decision algorithms. To date, immunohistochemistry and fluorescence in situ hybridization are the two p…
View article: Evaluation of Micro Satellite Instability and Mismatch Repair Status in Different Solid Tumors: A Multicenter Analysis in a Real World Setting
Evaluation of Micro Satellite Instability and Mismatch Repair Status in Different Solid Tumors: A Multicenter Analysis in a Real World Setting Open
Immune-checkpoint inhibitors (ICIs) play a key role in the treatment of advanced stage colorectal cancer (CRC) patients featuring a deficient DNA mismatch repair (dMMR) system or a high microsatellite instability (MSI-H) profile. However, …
View article: Liquid Biopsy Analysis in Clinical Practice: Focus on Lung Cancer
Liquid Biopsy Analysis in Clinical Practice: Focus on Lung Cancer Open
Lung cancer is the leading cause of cancer death worldwide. Despite the emergence of highly effective targeted therapies, up to 30% of advanced stage non-small cell lung cancer (NSCLC) patients do not undergo tissue molecular testing becau…
View article: Molecular Testing of Thyroid Fine-Needle Aspiration: Local Issues and Solutions. An Interventional Cytopathologist Perspective
Molecular Testing of Thyroid Fine-Needle Aspiration: Local Issues and Solutions. An Interventional Cytopathologist Perspective Open
Molecular testing has acquired a relevant role for diagnostic and prognostic stratification of indeterminate thyroid nodules. Besides the available commercial solutions marketed in the United States, various local testing strategies have b…
View article: Liquid Biopsy Is a Promising Tool for Genetic Testing in Idiopathic Pulmonary Fibrosis
Liquid Biopsy Is a Promising Tool for Genetic Testing in Idiopathic Pulmonary Fibrosis Open
Liquid biopsy, which allows the isolation of circulating cell-free (ccf) DNA from blood, is an emerging noninvasive tool widely used in oncology for diagnostic and prognosis purposes. Previous data have shown that serum cfDNA discriminates…
View article: Next generation sequencing in cytology
Next generation sequencing in cytology Open
The application of next generation sequencing (NGS) technology to cytological samples has significantly modified molecular cytopathology practice. Cytological samples represent a valid source of high‐quality DNA for NGS analysis, especiall…
View article: Next Generation Sequencing in Cytopathology: Focus on Non-Small Cell Lung Cancer
Next Generation Sequencing in Cytopathology: Focus on Non-Small Cell Lung Cancer Open
Molecular cytopathology is a rapidly evolving field embracing both conventional microscopy and molecular pathology. Its growing popularity stems from the fact that in many types of advanced cancers, including non small cell lung cancer (NS…
View article: RNA-Based Assay for Next-Generation Sequencing of Clinically Relevant Gene Fusions in Non-Small Cell Lung Cancer
RNA-Based Assay for Next-Generation Sequencing of Clinically Relevant Gene Fusions in Non-Small Cell Lung Cancer Open
Gene fusions represent novel predictive biomarkers for advanced non-small cell lung cancer (NSCLC). In this study, we validated a narrow NGS gene panel able to cover therapeutically-relevant gene fusions and splicing events in advanced-sta…
View article: Tumor mutational burden on cytological samples: A pilot study
Tumor mutational burden on cytological samples: A pilot study Open
Background Immune‐checkpoint inhibitors (ICIs) represent an important treatment option for patients who have advanced stage non–small cell lung cancer (NSCLC). Currently, evaluation of the expression level of programmed death‐ligand 1 (PD‐…
View article: Thyroid fine-needle aspiration trends before, during, and after the lockdown: what we have learned so far from the COVID-19 pandemic
Thyroid fine-needle aspiration trends before, during, and after the lockdown: what we have learned so far from the COVID-19 pandemic Open
View article: BRAF: A Two-Faced Janus
BRAF: A Two-Faced Janus Open
Gain-of-function of V-Raf Murine Sarcoma Viral Oncogene Homolog B (BRAF) is one of the most frequent oncogenic mutations in numerous cancers, including thyroid papillary carcinoma, melanoma, colon, and lung carcinomas, and to a lesser exte…
View article: KRAS mutations testing in non-small cell lung cancer: the role of Liquid biopsy in the basal setting
KRAS mutations testing in non-small cell lung cancer: the role of Liquid biopsy in the basal setting Open
In advanced stage non-small cell lung cancer (NSCLC) patients, Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) testing may soon acquire a predictive significance to select patients for AMG510 treatment. Since tissue samples are no…
View article: Harmonization of Next-Generation Sequencing Procedure in Italian Laboratories: A Multi-Institutional Evaluation of the SiRe® Panel
Harmonization of Next-Generation Sequencing Procedure in Italian Laboratories: A Multi-Institutional Evaluation of the SiRe® Panel Open
Background: Next-generation sequencing (NGS) needs to be validated and standardized to ensure that cancer patients are reliably selected for target treatments. In Italy, NGS is performed in several institutions and harmonization of …
View article: Analysis of Programmed Death-Ligand 1 Expression, Stromal Tumor-Infiltrating Lymphocytes, and Mismatch Repair Deficiency in Invasive Micropapillary Carcinoma of the Breast
Analysis of Programmed Death-Ligand 1 Expression, Stromal Tumor-Infiltrating Lymphocytes, and Mismatch Repair Deficiency in Invasive Micropapillary Carcinoma of the Breast Open
Introduction: Invasive micropapillary carcinoma (IMPC) of the breast is a rare and aggressive subtype of invasive ductal carcinomas, associated with poor prognosis and without a well-established treatment. Programmed death-ligand 1 (PD-L1)…
View article: Cell free DNA analysis by SiRe® next generation sequencing panel in non small cell lung cancer patients: focus on basal setting
Cell free DNA analysis by SiRe® next generation sequencing panel in non small cell lung cancer patients: focus on basal setting Open
SiRe® NGS panel represent an effective diagnostic tool in cfDNA analysis setting.
View article: Young investigator challenge: Can the Ion AmpliSeq Cancer Hotspot Panel v2 be used for next‐generation sequencing of thyroid FNA samples?
Young investigator challenge: Can the Ion AmpliSeq Cancer Hotspot Panel v2 be used for next‐generation sequencing of thyroid FNA samples? Open
BACKGROUND Fine‐needle aspiration (FNA) cytology is accurate and cost‐effective in the evaluation of thyroid nodules. Molecular techniques may contribute to risk stratification in indeterminate cases. Although next‐generation sequencing (N…