Mariko Ohnuma
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Peculiar properties of tuber starch in a potato mutant lacking the α-glucan water dikinase 1 gene <i>GWD1</i> created by targeted mutagenesis using the CRISPR/dMac3-Cas9 system Open
Glucose chains in starch are phosphorylated and contribute to structural stabilization. Phosphate groups contained in starch also play a role in retaining moisture. α-Glucan water dikinase 1 (GWD1) is involved in the phosphorylation of glu…
Procedure for the efficient acquisition of progeny seeds from crossed potato plants grafted onto tomato Open
Potato, Solanum tuberosum L. is an important crop. However, it is difficult to breed potato cultivars by applying conventional crossing methods because potato has a tetraploid genome and is vegetatively propagated. Flower formation and tub…
Creation of a potato mutant lacking the starch branching enzyme gene <i>StSBE3</i> that was generated by genome editing using the CRISPR/dMac3-Cas9 system Open
The potato tuber starch trait is changed depending on the composition of amylose and amylopectin. The amount of amylopectin is determined by the activity of the starch branching enzymes SBE1, SBE2, and SBE3 in potato. SBE3, a homolog of ri…
A simple method to establish an efficient medium suitable for potato regeneration Open
Potato (Solanum tuberosum) is one of the important crop plants, and many potato cultivars consist of a tetraploid genome with high heterozygosity. The techniques of transformation and genome editing require plant regeneration. However, no …
Establishment of a conditional TALEN system using the translational enhancer dMac3 and an inducible promoter activated by glucocorticoid treatment to increase the frequency of targeted mutagenesis in plants Open
Transcription activator-like effector nuclease (TALEN) is an artificial nuclease that causes DNA cleavage at the target site and induces few off-target reactions because of its high sequence specificity. Powerful and variable tools using T…
Establishment of a modified CRISPR/Cas9 system with increased mutagenesis frequency using the translational enhancer dMac3 and multiple guide RNAs in potato Open
CRISPR/Cas9 is a programmable nuclease composed of the Cas9 protein and a guide RNA (gRNA) molecule. To create a mutant potato, a powerful genome-editing system was required because potato has a tetraploid genome. The translational enhance…
WIND1 Promotes Shoot Regeneration through Transcriptional Activation of <i>ENHANCER OF SHOOT REGENERATION1</i> in Arabidopsis Open
Many plant species display remarkable developmental plasticity and regenerate new organs after injury. Local signals produced by wounding are thought to trigger organ regeneration but molecular mechanisms underlying this control remain lar…