Marius Gramlich
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View article: Two birds with one stone: human SIRPα nanobodies for functional modulation and in vivo imaging of myeloid cells
Two birds with one stone: human SIRPα nanobodies for functional modulation and in vivo imaging of myeloid cells Open
Signal-regulatory protein α (SIRPα) expressed by myeloid cells is of particular interest for therapeutic strategies targeting the interaction between SIRPα and the “don’t eat me” ligand CD47 and as a marker to monitor macrophage infiltrati…
View article: Development of a PNGase Rc Column for Online Deglycosylation of Complex Glycoproteins during HDX-MS
Development of a PNGase Rc Column for Online Deglycosylation of Complex Glycoproteins during HDX-MS Open
Protein glycosylation is one of the most common PTMs and many cell surface receptors, extracellular proteins, and biopharmaceuticals are glycosylated. However, HDX-MS analysis of such important glycoproteins has so far been limited by diff…
View article: Development of a PNGase Rc column for online deglycosylation of complex glycoproteins during HDX-MS
Development of a PNGase Rc column for online deglycosylation of complex glycoproteins during HDX-MS Open
Protein glycosylation is one of the most common PTMs and many cell surface receptors, extracellular proteins and biopharmaceuticals are glycosylated. However, HDX-MS analysis of such important glycoproteins has so far been limited by diffi…
View article: Two birds with one stone: human SIRPα nanobodies for functional modulation and in vivo imaging of myeloid cells
Two birds with one stone: human SIRPα nanobodies for functional modulation and in vivo imaging of myeloid cells Open
Signal-regulatory protein α (SIRPα) expressed by myeloid cells is of particular interest for therapeutic strategies targeting the interaction between SIRPα and the "don’t eat me" ligand CD47 and as a marker to monitor macrophage infiltrati…
View article: A novel PNGase Rc for improved protein N-deglycosylation in bioanalytics and HDX-MS epitope mapping under challenging conditions
A novel PNGase Rc for improved protein N-deglycosylation in bioanalytics and HDX-MS epitope mapping under challenging conditions Open
N-linked glycosylation is a ubiquitous posttranslational modification of proteins. While it plays an important role in the biological function of proteins, it often poses a major challenge for their analytical characterization. Currently a…
View article: Single-Domain Antibodies for Targeting, Detection, and In Vivo Imaging of Human CD4+ Cells
Single-Domain Antibodies for Targeting, Detection, and In Vivo Imaging of Human CD4+ Cells Open
The advancement of new immunotherapies necessitates appropriate probes to monitor the presence and distribution of distinct immune cell populations. Considering the key role of CD4 + cells in regulating immunological processes, we generate…
View article: Single-domain antibodies for targeting, detection and<i>in vivo</i>imaging of human CD4<sup>+</sup>cells
Single-domain antibodies for targeting, detection and<i>in vivo</i>imaging of human CD4<sup>+</sup>cells Open
The advancement of new immunotherapies necessitates appropriate probes to monitor the presence and distribution of distinct immune cell populations. Considering the key role of CD4 + T cells in regulating immunological processes, we genera…
View article: NeutrobodyPlex—monitoring SARS‐CoV‐2 neutralizing immune responses using nanobodies
NeutrobodyPlex—monitoring SARS‐CoV‐2 neutralizing immune responses using nanobodies Open
In light of the COVID-19 pandemic, there is an ongoing need for diagnostic tools to monitor the immune status of large patient cohorts and the effectiveness of vaccination campaigns. Here, we present 11 unique nanobodies (Nbs) specific for…
View article: HDX-MS for Epitope Characterization of a Therapeutic ANTIBODY Candidate on the Calcium-Binding Protein Annexin-A1
HDX-MS for Epitope Characterization of a Therapeutic ANTIBODY Candidate on the Calcium-Binding Protein Annexin-A1 Open
Annexin-A1 (ANXA1) belongs to a class of highly homologous Ca2+-dependent phospholipid-binding proteins. Its structure consists of a core region composed of four homologous repeats arranged in a compact, hydrolysis-resistant structure and …
View article: NeutrobodyPlex - Nanobodies to monitor a SARS-CoV-2 neutralizing immune response
NeutrobodyPlex - Nanobodies to monitor a SARS-CoV-2 neutralizing immune response Open
As the COVID-19 pandemic escalates, the need for effective vaccination programs, therapeutic intervention, and diagnostic tools increases. Here, we identified 11 unique nanobodies (Nbs) specific for the SARS-CoV-2 spike receptor-binding do…
View article: Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes
Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes Open
Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52) are indispensable tools in releasing N-glycans from glycoproteins. So far, only a limited number of PNGas…