Marius Horch
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View article: Two-dimensional infrared spectroscopy as a tool to reveal the vibrational and molecular structure of [FeFe] hydrogenases
Two-dimensional infrared spectroscopy as a tool to reveal the vibrational and molecular structure of [FeFe] hydrogenases Open
In this proof-of-concept study, we demonstrate how experimental and computational 2D-IR spectroscopy can provide insights into the vibrational and molecular structure of [FeFe] hydrogenases.
View article: Expanding the scope of resonance Raman spectroscopy in hydrogenase research: New observable states and reporter vibrations
Expanding the scope of resonance Raman spectroscopy in hydrogenase research: New observable states and reporter vibrations Open
Oxygen-tolerant [NiFe] hydrogenases are valuable blueprints for the activation and evolution of molecular hydrogen under application-relevant conditions. Vibrational spectroscopic techniques play a key role in the investigation of these me…
View article: Light‐Induced Electron Transfer in a [NiFe] Hydrogenase Opens a Photochemical Shortcut for Catalytic Dihydrogen Cleavage
Light‐Induced Electron Transfer in a [NiFe] Hydrogenase Opens a Photochemical Shortcut for Catalytic Dihydrogen Cleavage Open
[NiFe] hydrogenases catalyze the reversible cleavage of molecular hydrogen into protons and electrons. Here, we have studied the impact of temperature and illumination on an oxygen‐tolerant and thermostable [NiFe] hydrogenase by IR and EPR…
View article: Lichtinduzierter Elektronentransfer in einer [NiFe]‐Hydrogenase: Eine Photochemische Abkürzung für die Katalytische Wasserstoffspaltung
Lichtinduzierter Elektronentransfer in einer [NiFe]‐Hydrogenase: Eine Photochemische Abkürzung für die Katalytische Wasserstoffspaltung Open
[NiFe]‐Hydrogenasen katalysieren die reversible Spaltung von molekularem Wasserstoff in Protonen und Elektronen. Hier untersuchen wir mittels IR‐ und EPR‐Spektroskopie den Einfluß von Temperatur und Licht auf eine sauerstofftolerante und t…
View article: Understanding the [NiFe] Hydrogenase Active Site Environment through Ultrafast Infrared and 2D-IR Spectroscopy of the Subsite Analogue K[CpFe(CO)(CN)<sub>2</sub>] in Polar and Protic Solvents
Understanding the [NiFe] Hydrogenase Active Site Environment through Ultrafast Infrared and 2D-IR Spectroscopy of the Subsite Analogue K[CpFe(CO)(CN)<sub>2</sub>] in Polar and Protic Solvents Open
The [CpFe(CO)(CN)2]− unit is an excellent structural model for the Fe(CO)(CN)2 moiety of the active site found in [NiFe] hydrogenases. Ultrafast infrared (IR) pump–probe and 2D-IR spectroscopy have been used to study K[CpFe(CO)(CN)2] (M1) …
View article: Understanding 2D-IR Spectra of Hydrogenases: A Descriptive and Predictive Computational Study
Understanding 2D-IR Spectra of Hydrogenases: A Descriptive and Predictive Computational Study Open
[NiFe] hydrogenases are metalloenzymes that catalyze the reversible cleavage of dihydrogen (H2), a clean future fuel. Understanding the mechanism of these biocatalysts requires spectroscopic techniques that yield insights into the structur…
View article: Ultrafast 2D-IR spectroscopy of [NiFe] hydrogenase from <i>E. coli</i> reveals the role of the protein scaffold in controlling the active site environment
Ultrafast 2D-IR spectroscopy of [NiFe] hydrogenase from <i>E. coli</i> reveals the role of the protein scaffold in controlling the active site environment Open
Ultrafast two-dimensional infrared (2D-IR) spectroscopy of Escherichia coli Hyd-1 ( Ec Hyd-1) reveals the structural and dynamic influence of the protein scaffold on the Fe(CO)(CN) 2 unit of the active site.
View article: Ein neuer Aufbau zur Untersuchung der Struktur und Funktion von solvatisierten, lyophilisierten und kristallinen Metalloenzymen – veranschaulicht anhand von [NiFe]‐Hydrogenasen
Ein neuer Aufbau zur Untersuchung der Struktur und Funktion von solvatisierten, lyophilisierten und kristallinen Metalloenzymen – veranschaulicht anhand von [NiFe]‐Hydrogenasen Open
Zur Untersuchung von Metalloenzymen haben wir einen Aufbau für die Präparation katalytischer Intermediate und deren anschließende Charakterisierung mit spektroskopischen Techniken entwickelt. Mit diesem können Redoxreaktionen in Enzymen in…
View article: Ein neuer Aufbau zur Untersuchung der Struktur und Funktion von solvatisierten, lyophilisierten und kristallinen Metalloenzymen – veranschaulicht anhand von [NiFe]‐Hydrogenasen
Ein neuer Aufbau zur Untersuchung der Struktur und Funktion von solvatisierten, lyophilisierten und kristallinen Metalloenzymen – veranschaulicht anhand von [NiFe]‐Hydrogenasen Open
Zur Untersuchung von Metalloenzymen haben wir einen Aufbau für die Präparation katalytischer Intermediate und deren anschließende Charakterisierung mit spektroskopischen Techniken entwickelt. Mit diesem können Redoxreaktionen in Enzymen in…
View article: Exploring Structure and Function of Redox Intermediates in [NiFe]‐Hydrogenases by an Advanced Experimental Approach for Solvated, Lyophilized and Crystallized Metalloenzymes
Exploring Structure and Function of Redox Intermediates in [NiFe]‐Hydrogenases by an Advanced Experimental Approach for Solvated, Lyophilized and Crystallized Metalloenzymes Open
To study metalloenzymes in detail, we developed a new experimental setup allowing the controlled preparation of catalytic intermediates for characterization by various spectroscopic techniques. The in situ monitoring of redox transitions b…
View article: Shedding Light on Proton and Electron Dynamics in [FeFe] Hydrogenases
Shedding Light on Proton and Electron Dynamics in [FeFe] Hydrogenases Open
[FeFe] hydrogenases are highly efficient catalysts for reversible dihydrogen evolution. H2 turnover involves different catalytic intermediates including a recently characterized hydride state of the active site (H-cluster). Applying cryoge…
View article: X‐ray Crystallography and Vibrational Spectroscopy Reveal the Key Determinants of Biocatalytic Dihydrogen Cycling by [NiFe] Hydrogenases
X‐ray Crystallography and Vibrational Spectroscopy Reveal the Key Determinants of Biocatalytic Dihydrogen Cycling by [NiFe] Hydrogenases Open
[NiFe] hydrogenases are complex model enzymes for the reversible cleavage of dihydrogen (H 2 ). However, structural determinants of efficient H 2 binding to their [NiFe] active site are not properly understood. Here, we present crystallogr…
View article: X‐ray Crystallography and Vibrational Spectroscopy Reveal the Key Determinants of Biocatalytic Dihydrogen Cycling by [NiFe] Hydrogenases
X‐ray Crystallography and Vibrational Spectroscopy Reveal the Key Determinants of Biocatalytic Dihydrogen Cycling by [NiFe] Hydrogenases Open
[NiFe] hydrogenases are complex model enzymes for the reversible cleavage of dihydrogen (H 2 ). However, structural determinants of efficient H 2 binding to their [NiFe] active site are not properly understood. Here, we present crystallogr…
View article: Understanding the structure and dynamics of hydrogenases by ultrafast and two-dimensional infrared spectroscopy
Understanding the structure and dynamics of hydrogenases by ultrafast and two-dimensional infrared spectroscopy Open
A proof-of-concept study on a catalytic [NiFe] intermediate reveals structural and dynamical details of hydrogenases by ultrafast and two-dimensional infrared spectroscopies.
View article: Enzymatic and spectroscopic properties of a thermostable [NiFe]‑hydrogenase performing H2-driven NAD+-reduction in the presence of O2
Enzymatic and spectroscopic properties of a thermostable [NiFe]‑hydrogenase performing H2-driven NAD+-reduction in the presence of O2 Open
View article: CCDC 1515404: Experimental Crystal Structure Determination
CCDC 1515404: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: CCDC 1515403: Experimental Crystal Structure Determination
CCDC 1515403: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: Vibrational spectroscopy reveals the initial steps of biological hydrogen evolution
Vibrational spectroscopy reveals the initial steps of biological hydrogen evolution Open
Low temperature resonance Raman spectroscopy reveals the initial, transient H-cluster intermediate during biological hydrogen production by [FeFe]-hydrogenase.
View article: Domain motions and electron transfer dynamics in 2Fe-superoxide reductase
Domain motions and electron transfer dynamics in 2Fe-superoxide reductase Open
Theoretical studies on 2Fe-superoxide reductase provide mechanistic insights into structural dynamics and electron transfer efficiencies.
View article: Orientation-Controlled Electrocatalytic Efficiency of an Adsorbed Oxygen-Tolerant Hydrogenase
Orientation-Controlled Electrocatalytic Efficiency of an Adsorbed Oxygen-Tolerant Hydrogenase Open
Protein immobilization on electrodes is a key concept in exploiting enzymatic processes for bioelectronic devices. For optimum performance, an in-depth understanding of the enzyme-surface interactions is required. Here, we introduce an int…
View article: Structure-function relationships of metalloenzymes
Structure-function relationships of metalloenzymes Open
Anhand der Modellsysteme [NiFe] Hydrogenase und Superoxid Reduktase wurden Struktur-Funktionsbeziehungen von Metalloenzymen, die die Umwandlung kleiner Moleküle katalysieren, mittels theoretischer und spektroskopischer Methoden analysiert.…
View article: 2<sup>nd</sup> coordination sphere controlled electron transfer of iron hangman complexes on electrodes probed by surface enhanced vibrational spectroscopy
2<sup>nd</sup> coordination sphere controlled electron transfer of iron hangman complexes on electrodes probed by surface enhanced vibrational spectroscopy Open
Surface enhanced vibrational spectroscopy shows the correlation between electron transfer kinetics and protonation degree of Fe Hangman complexes on electrodes.
View article: Concepts in bio-molecular spectroscopy: vibrational case studies on metalloenzymes
Concepts in bio-molecular spectroscopy: vibrational case studies on metalloenzymes Open
Challenges and chances in bio-molecular spectroscopy are exemplified by vibrational case studies on metalloenzymes.