Mark A. Eiteman
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View article: Increased Mevalonate Production Using Engineered Citrate Synthase and Phosphofructokinase Variants of <i>Escherichia coli</i>
Increased Mevalonate Production Using Engineered Citrate Synthase and Phosphofructokinase Variants of <i>Escherichia coli</i> Open
Mevalonate is a biochemical precursor to a wide range of isoprenoids. The mevalonate pathway uses three moles of acetyl‐CoA, and therefore native pathways which metabolize acetyl‐CoA compete with mevalonate synthesis. Moreover, the final s…
View article: Production of Esters in Escherichia coli Using Citrate Synthase Variants
Production of Esters in Escherichia coli Using Citrate Synthase Variants Open
Acetate esters comprise a wide range of products including fragrances and industrial solvents. Biosynthesis of esters offers a promising alternative to chemical synthesis because such routes use renewable carbohydrate resources and minimiz…
View article: Citrate synthase variants improve yield of acetyl-CoA derived 3-hydroxybutyrate in Escherichia coli
Citrate synthase variants improve yield of acetyl-CoA derived 3-hydroxybutyrate in Escherichia coli Open
Background The microbial chiral product (R)-3-hydroxybutyrate (3-HB) is a gateway to several industrial and medical compounds. Acetyl-CoA is the key precursor for 3-HB, and several native pathways compete with 3-HB production. The principa…
View article: Pyruvic Acid Production from Sucrose by Escherichia coli Pyruvate Dehydrogenase Variants
Pyruvic Acid Production from Sucrose by Escherichia coli Pyruvate Dehydrogenase Variants Open
Sucrose is an abundant, cheap, and renewable carbohydrate which makes it an attractive feedstock for the biotechnological production of chemicals. Escherichia coli W, one of the few safe E. coli strains able to metabolize sucrose, was exam…
View article: <i>Escherichia coli aceE</i> variants coding pyruvate dehydrogenase improve the generation of pyruvate‐derived acetoin
<i>Escherichia coli aceE</i> variants coding pyruvate dehydrogenase improve the generation of pyruvate‐derived acetoin Open
Several chromosomally expressed AceE variants were constructed in Escherichia coli ΔldhA ΔpoxB ΔppsA and compared using glucose as the sole carbon source. These variants were examined in shake flask cultures for growth rate, pyruvate accum…
View article: Nutrient-Limited Operational Strategies for the Microbial Production of Biochemicals
Nutrient-Limited Operational Strategies for the Microbial Production of Biochemicals Open
Limiting an essential nutrient has a profound impact on microbial growth. The notion of growth under limited conditions was first described using simple Monod kinetics proposed in the 1940s. Different operational modes (chemostat, fed-batc…
View article: Isolation and Characterization of Levoglucosan-Metabolizing Bacteria
Isolation and Characterization of Levoglucosan-Metabolizing Bacteria Open
Levoglucosan is the most prevalent soluble carbohydrate remaining after high-temperature pyrolysis of lignocellulosic biomass, but it is not fermented by typical production microbes such as Escherichia coli and Saccharomyces cerevisiae . A…
View article: Pyruvate Production by Escherichia coli by Use of Pyruvate Dehydrogenase Variants
Pyruvate Production by Escherichia coli by Use of Pyruvate Dehydrogenase Variants Open
Microbial production of biochemicals from renewable resources has become an efficient and cost-effective alternative to traditional chemical synthesis methods. Metabolic engineering tools are important for optimizing a process to perform a…
View article: Biotechnology and Bioengineering: Volume 117, Number 12, December 2020
Biotechnology and Bioengineering: Volume 117, Number 12, December 2020 Open
Regula on and remodeling of intermediate metabolite and membrane lipid during NaCl-
View article: In vivo interpretation of model predicted inhibition in acrylate pathway engineered Lactococcus lactis
In vivo interpretation of model predicted inhibition in acrylate pathway engineered Lactococcus lactis Open
In order to maximize the productivity of engineered metabolic pathway, in silico model is an established means to provide features of enzyme reaction dynamics. In our previous study, E.coli engineered with acrylate pathway yielded low prop…
View article: Acetate formation during recombinant protein production in <i>Escherichia coli</i> K‐12 with an elevated NAD(H) pool
Acetate formation during recombinant protein production in <i>Escherichia coli</i> K‐12 with an elevated NAD(H) pool Open
Acetate formation is a disadvantage in the use of Escherichia coli for recombinant protein production, and many studies have focused on optimizing fermentation processes or altering metabolism to eliminate acetate accumulation. In this stu…
View article: Accelerating pathway evolution by increasing the gene dosage of chromosomal segments
Accelerating pathway evolution by increasing the gene dosage of chromosomal segments Open
Significance Gene duplication and divergence are cornerstones of evolution. Genetic redundancy resulting from repeated DNA provides flexibility for transient changes in copy number that may confer selective benefit under new or changing en…
View article: Conversion of glucose‐xylose mixtures to pyruvate using a consortium of metabolically engineered <i>Escherichia coli</i>
Conversion of glucose‐xylose mixtures to pyruvate using a consortium of metabolically engineered <i>Escherichia coli</i> Open
Two strains of Escherichia coli were engineered to accumulate pyruvic acid from two sugars found in lignocellulosic hydrolysates by knockouts in the aceE , ppsA , poxB , and ldhA genes. Additionally, since glucose and xylose are typically …
View article: Recent Progress in the Microbial Production of Pyruvic Acid
Recent Progress in the Microbial Production of Pyruvic Acid Open
Pyruvic acid (pyruvate) is a cellular metabolite found at the biochemical junction of glycolysis and the tricarboxylic acid cycle. Pyruvate is used in food, cosmetics, pharmaceutical and agricultural applications. Microbial production of p…
View article: Phosphatases and phosphate affect the formation of glucose from pentoses in <i>Escherichia coli</i>
Phosphatases and phosphate affect the formation of glucose from pentoses in <i>Escherichia coli</i> Open
Metabolically engineered Escherichia coli MEC143 with deletions of the ptsG , manZ , glk , pfkA , and zwf genes converts pentoses such as arabinose and xylose into glucose, with the dephosphorylation of glucose‐6‐phosphate serving as the f…