Mark Castleberry
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View article: Post-transcriptional modifications on tRNA fragments confer functional changes to high-density lipoproteins in atherosclerosis
Post-transcriptional modifications on tRNA fragments confer functional changes to high-density lipoproteins in atherosclerosis Open
Results support a model in which HDL-delivered m1A-HDL-tDRs regulate immune signaling, macrophage activation, and pro-inflammatory sub-phenotypes within the atherosclerotic lesion.
View article: Transcriptional programming by GATA3 regulates CD4+ T cell GM-CSF production in the gastrointestinal tract during graft versus host disease
Transcriptional programming by GATA3 regulates CD4+ T cell GM-CSF production in the gastrointestinal tract during graft versus host disease Open
Acute graft versus host disease (GVHD) of the gastrointestinal (GI) tract is a major complication of allogeneic hematopoietic stem cell marrow transplantation (HSCT). CD4+ T cells that produce GM-CSF have emerged as central mediators of in…
A comprehensive analysis of supermere, exomere, and extracellular vesicle isolation and cargo in colorectal cancer Open
Biofluids contain a heterogeneous mixture of extracellular vesicles and non-vesicular nanoparticles (including exomeres and supermeres) that transport a diverse array of proteins, RNA, and lipids. Our previous efforts to characterize the c…
View article: Elucidating brain transport pathways and cell type-dependent gene silencing of a durable lipid–siRNA conjugate administered into cerebrospinal fluid
Elucidating brain transport pathways and cell type-dependent gene silencing of a durable lipid–siRNA conjugate administered into cerebrospinal fluid Open
The clinical neurosciences are in the midst of a renaissance spurred by the development of new therapeutic modalities. Short interfering RNAs (siRNAs), in particular, are gaining interest for treating neurological diseases owing to their c…
View article: Post-transcriptional modifications on tRNA fragments confer functional changes to high-density lipoproteins in atherosclerosis
Post-transcriptional modifications on tRNA fragments confer functional changes to high-density lipoproteins in atherosclerosis Open
SUMMARY Epitranscriptomic modifications on RNA play critical roles in stability, processing, and function, partly by influencing interactions with RNA-binding proteins and receptors. The role of post-transcriptional RNA modifications on ce…
View article: Lipid-siRNA conjugate accesses a perivascular transport mechanism and achieves widespread and durable knockdown in the central nervous system
Lipid-siRNA conjugate accesses a perivascular transport mechanism and achieves widespread and durable knockdown in the central nervous system Open
Short-interfering RNA (siRNA) has gained significant interest for treatment of neurological diseases by providing the capacity to achieve sustained inhibition of nearly any gene target. Yet, efficacious drug delivery throughout deep brain …
View article: Oxidized LDL regulates efferocytosis through the CD36-PKM2-mtROS pathway
Oxidized LDL regulates efferocytosis through the CD36-PKM2-mtROS pathway Open
Macrophage efferocytosis, the process by which phagocytes engulf and remove apoptotic cells (ACs), plays a critical role in maintaining tissue homeostasis. Efficient efferocytosis prevents secondary necrosis, mitigates chronic inflammation…
Intracellular tPA–PAI-1 interaction determines VLDL assembly in hepatocytes Open
Apolipoprotein B (apoB)–lipoproteins initiate and promote atherosclerotic cardiovascular disease. Plasma tissue plasminogen activator (tPA) activity is negatively associated with atherogenic apoB-lipoprotein cholesterol levels in humans, b…
Anapole-Assisted Low-Power Optical Trapping of Nanoscale Extracellular Vesicles and Particles Open
This study addresses the challenge of trapping nanoscale biological particles using optical tweezers without the photothermal heating effect and the limitation presented by the diffraction limit. Optical tweezers are effective for trapping…
Elucidation of physico-chemical principles of high-density lipoprotein–small RNA binding interactions Open
Extracellular small RNAs (sRNAs) are abundant in many biofluids, but little is known about their mechanisms of transport and stability in RNase-rich environments. We previously reported that high-density lipoproteins (HDLs) in mice were en…
View article: Physico-chemical principles of HDL-small RNA binding interactions
Physico-chemical principles of HDL-small RNA binding interactions Open
Extracellular small RNAs (sRNA) are abundant in many biofluids, but little is known about their mechanisms of transport and stability in RNase-rich environments. We previously reported that high-density lipoproteins (HDL) of mice were enri…
View article: LDL delivery of microbial small RNAs drives atherosclerosis through macrophage TLR8
LDL delivery of microbial small RNAs drives atherosclerosis through macrophage TLR8 Open
Macrophages present a spectrum of phenotypes that mediate both the pathogenesis and resolution of atherosclerotic lesions. Inflammatory macrophage phenotypes are pro-atherogenic, but the natural factors that instigate this polarization are…
Functional recombinant apolipoprotein A5 that is stable at high concentrations at physiological pH Open
APOA5 is a low-abundance exchangeable apolipoprotein that plays critical roles in human triglyceride (TG) metabolism. Indeed, aberrations in the plasma concentration or structure of APOA5 are linked to hypertriglyceridemia, hyperchylomicro…
View article: An Evaluation of the Crystal Structure of C-terminal Truncated Apolipoprotein A-I in Solution Reveals Structural Dynamics Related to Lipid Binding
An Evaluation of the Crystal Structure of C-terminal Truncated Apolipoprotein A-I in Solution Reveals Structural Dynamics Related to Lipid Binding Open
Apolipoprotein (apo) A-I mediates many of the anti-atherogenic functions attributed to high density lipoprotein. Unfortunately, efforts toward a high resolution structure of full-length apoA-I have not been fruitful, although there have be…