Mark Gomelsky
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View article: Maple polyphenols inhibit sortase and drastically reduce <i>Streptococcus mutans</i> biofilms
Maple polyphenols inhibit sortase and drastically reduce <i>Streptococcus mutans</i> biofilms Open
Dental caries is initiated by the adherence of Streptococcus mutans to tooth surfaces, leading to biofilm (plaque) formation and the production of enamel-eroding acids. Sortase A (SrtA) plays a critical role in this process because it anch…
View article: Maple compounds prevent biofilm formation in Listeria monocytogenes via sortase inhibition
Maple compounds prevent biofilm formation in Listeria monocytogenes via sortase inhibition Open
The Pss exopolysaccharide (EPS) enhances the ability of the foodborne pathogen Listeria monocytogenes to colonize and persist on surfaces of fresh fruits and vegetables. Eradicating listeria within EPS-rich biofilms is challenging due to t…
View article: Robust inducible gene expression in intracellular<i>Listeria monocytogenes in vivo</i>
Robust inducible gene expression in intracellular<i>Listeria monocytogenes in vivo</i> Open
Attenuated strains of the intracellular pathogen Listeria monocytogenes can deliver genetically encoded payloads inside tumor cells. L. monocytogenes preferentially accumulates and propagates inside immune-suppressed tumor microenvironment…
View article: Lignan-containing maple products inhibit Listeria monocytogenes biofilms on fresh produce
Lignan-containing maple products inhibit Listeria monocytogenes biofilms on fresh produce Open
Major listeriosis outbreaks have been associated with fresh produce contaminated with Listeria monocytogenes . Strains that synthesize the Pss exopolysaccharide (EPS) have an estimated 10 2 to 10 4 -fold advantage over nonsynthesizing stra…
View article: The Listeria monocytogenes exopolysaccharide significantly enhances colonization and survival on fresh produce
The Listeria monocytogenes exopolysaccharide significantly enhances colonization and survival on fresh produce Open
Fresh produce contaminated with Listeria monocytogenes has caused major listeriosis outbreaks in the last decades. Our knowledge about components of the listerial biofilms formed on fresh produce and their roles in causing foodborne illnes…
View article: Coordinated control of the type IV pili and c‐di‐GMP‐dependent antifungal antibiotic production in <i>L</i><i>ysobacter</i> by the response regulator PilR
Coordinated control of the type IV pili and c‐di‐GMP‐dependent antifungal antibiotic production in <i>L</i><i>ysobacter</i> by the response regulator PilR Open
In the soil gammaproteobacterium Lysobacter enzymogenes , a natural fungal predator, the response regulator PilR controls type IV pili (T4P)‐mediated twitching motility as well as synthesis of the heat‐stable antifungal factor (HSAF). Earl…
View article: A YajQ‐LysR‐like, cyclic di‐GMP‐dependent system regulating biosynthesis of an antifungal antibiotic in a crop‐protecting bacterium, <i>Lysobacter enzymogenes</i>
A YajQ‐LysR‐like, cyclic di‐GMP‐dependent system regulating biosynthesis of an antifungal antibiotic in a crop‐protecting bacterium, <i>Lysobacter enzymogenes</i> Open
Summary YajQ, a binding protein of the universal bacterial second messenger cyclic di‐GMP (c‐di‐GMP), affects virulence in several bacterial pathogens, including Xanthomonas campestris . In this bacterium, YajQ interacts with the transcrip…
View article: Cyclic di-GMP-Mediated Regulation of Gene Transfer and Motility in Rhodobacter capsulatus
Cyclic di-GMP-Mediated Regulation of Gene Transfer and Motility in Rhodobacter capsulatus Open
Gene transfer agents (GTAs) are virus-like particles that move cellular DNA between cells. In the alphaproteobacterium Rhodobacter capsulatus , GTA production is affected by the activities of multiple cellular regulatory systems, to which …
View article: Engineering Adenylate Cyclase Activated by Near-Infrared Window Light for Mammalian Optogenetic Applications
Engineering Adenylate Cyclase Activated by Near-Infrared Window Light for Mammalian Optogenetic Applications Open
Light in the near-infrared optical window (NIRW) penetrates deep through mammalian tissues, including the skull and brain tissue. Here we engineered an adenylate cyclase (AC) activated by NIRW light (NIRW-AC) and suitable for mammalian app…
View article: Positive and Negative Regulation of Glycerol Utilization by the c-di-GMP Binding Protein PlzA in Borrelia burgdorferi
Positive and Negative Regulation of Glycerol Utilization by the c-di-GMP Binding Protein PlzA in Borrelia burgdorferi Open
The Lyme disease pathogen, Borrelia burgdorferi , has a simple cyclic dimeric GMP (c-di-GMP) signaling system essential for adaptation of the pathogen to the complicated tick environment. The c-di-GMP effector of B. burgdorferi , PlzA, has…
View article: Signaling specificity in the c-di-GMP-dependent network regulating antibiotic synthesis in Lysobacter
Signaling specificity in the c-di-GMP-dependent network regulating antibiotic synthesis in Lysobacter Open
Enzymes controlling intracellular second messengers in bacteria, such as c-di-GMP, often affect some but not other targets. How such specificity is achieved is understood only partially. Here, we present a novel mechanism that enables spec…
View article: Biofilm formation – what we can learn from recent developments
Biofilm formation – what we can learn from recent developments Open
Although biofilms have been observed early in the history of microbial research, their impact has only recently been fully recognized. Biofilm infections, which contribute to up to 80% of human microbial infections, are associated with com…
View article: CodY-Mediated c-di-GMP-Dependent Inhibition of Mammalian Cell Invasion in Listeria monocytogenes
CodY-Mediated c-di-GMP-Dependent Inhibition of Mammalian Cell Invasion in Listeria monocytogenes Open
Elevated levels of the second messenger c-di-GMP suppress virulence in diverse pathogenic bacteria, yet mechanisms are poorly characterized. In the foodborne pathogen Listeria monocytogenes , high c-di-GMP levels inhibit mammalian cell inv…
View article: Identification of bacterial guanylate cyclases
Identification of bacterial guanylate cyclases Open
Originally published in PROTEINS: Structure, Function, and Bioinformatics 2015;83(5): 799–804(DOI: 10.1002/prot.24769) There was an error in the legend for figure 2. The correct legend is shown below. Figure 2. The CRP/CRPG reporter system…
View article: Optogenetic Module for Dichromatic Control of c-di-GMP Signaling
Optogenetic Module for Dichromatic Control of c-di-GMP Signaling Open
Many aspects of bacterial physiology and behavior, including motility, surface attachment, and the cell cycle, are controlled by cyclic di-GMP (c-di-GMP)-dependent signaling pathways on the scale of seconds to minutes. Interrogation of suc…
View article: Optogenetic Manipulation of Cyclic Di-GMP (c-di-GMP) Levels Reveals the Role of c-di-GMP in Regulating Aerotaxis Receptor Activity in Azospirillum brasilense
Optogenetic Manipulation of Cyclic Di-GMP (c-di-GMP) Levels Reveals the Role of c-di-GMP in Regulating Aerotaxis Receptor Activity in Azospirillum brasilense Open
Bacterial chemotaxis receptors provide the sensory inputs that inform the direction of navigation in changing environments. Recently, we described the bacterial second messenger cyclic di-GMP (c-di-GMP) as a novel regulator of a subclass o…
View article: Lysobacter PilR, the Regulator of Type IV Pilus Synthesis, Controls Antifungal Antibiotic Production via a Cyclic di-GMP Pathway
Lysobacter PilR, the Regulator of Type IV Pilus Synthesis, Controls Antifungal Antibiotic Production via a Cyclic di-GMP Pathway Open
Lysobacter enzymogenes is a ubiquitous soil gammaproteobacterium that produces a broad-spectrum antifungal antibiotic, known as heat-stable antifungal factor (HSAF). To increase HSAF production for use against fungal crop diseases, it is i…