Mark Howarth
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View article: mRNA delivery of genetically encoded mosaic-8 pan-sarbecovirus RBD vaccines
mRNA delivery of genetically encoded mosaic-8 pan-sarbecovirus RBD vaccines Open
Summary Global health remains threatened by spillovers of zoonotic SARS-like betacoronaviruses (sarbecoviruses) that could be mitigated by a pan-sarbecovirus vaccine 1 . We described elicitation of potently neutralizing and cross-reactive …
View article: Split NeissLock with Spy-Acceleration Arms Mammalian Proteins for Anhydride-Mediated Cell Ligation
Split NeissLock with Spy-Acceleration Arms Mammalian Proteins for Anhydride-Mediated Cell Ligation Open
Reactive functional groups may be incorporated into proteins or may emerge from natural amino acids in exceptional architectures. Anhydride formation is triggered by calcium in the self-processing module (SPM) of Neisseria meningitidis<…
View article: NanoBondy reacting through NeissLock anhydride allows covalent immune cell decoration
NanoBondy reacting through NeissLock anhydride allows covalent immune cell decoration Open
Cell-surface conjugation has enormous therapeutic and research potential. Existing technologies for cell-surface modification are usually reversible, non-specific, or rely on genetic editing of target cells. Here we present the NanoBondy, …
View article: Computationally designed haemagglutinin with nanocage plug-and-display elicits pan-H5 influenza vaccine responses
Computationally designed haemagglutinin with nanocage plug-and-display elicits pan-H5 influenza vaccine responses Open
The increasing spread of highly pathogenic avian influenza (HPAI) A/H5 viruses poses a pandemic threat. Circulating clade 2.3.4.4b viruses have demonstrated rapid transcontinental dissemination, extensive reassortment, epizootic spread and…
View article: Mpox multiprotein virus-like nanoparticle vaccine induces neutralizing and protective antibodies in mice and non-human primates
Mpox multiprotein virus-like nanoparticle vaccine induces neutralizing and protective antibodies in mice and non-human primates Open
View article: Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development
Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development Open
Influenza virus neuraminidase is a crucial target for protective antibodies, yet the development of recombinant neuraminidase protein as a vaccine has been held back by instability and variable expression. We have taken a pragmatic approac…
View article: Author response: Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development
Author response: Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development Open
View article: Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development
Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development Open
Influenza neuraminidase is a crucial target for protective antibodies, yet the development of recombinant neuraminidase protein as a vaccine has been held back by instability and variable expression. We have taken a pragmatic approach to i…
View article: Author response: Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development
Author response: Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development Open
View article: Structure-guided loop grafting improves expression and stability of influenza neuraminidase for vaccine development
Structure-guided loop grafting improves expression and stability of influenza neuraminidase for vaccine development Open
Influenza virus neuraminidase (NA) is a crucial target for protective antibodies, yet the development of recombinant NA protein as a vaccine has been held back by instability and variable expression. We have taken a pragmatic approach to i…
View article: The SpyBLI cell-free pipeline for the rapid quantification of binding kinetics from crude samples
The SpyBLI cell-free pipeline for the rapid quantification of binding kinetics from crude samples Open
Accurate measurements of binding kinetics, encompassing equilibrium dissociation constant (K D ), association rate ( k on ), and dissociation rate ( k o, ), are critical for the development and optimisation of high-affinity binding protein…
View article: Matchmaking at the cell surface using bispecifics to put cells on their best behavior
Matchmaking at the cell surface using bispecifics to put cells on their best behavior Open
Intermolecular relationships at the cell surface dictate the behavior and regulatory network of cells. Such interactions often require precise spatial control for optimal response. By binding simultaneously to two different target sites, b…
View article: The SpyBLI cell-free pipeline for the rapid quantification of binding kinetics from crude samples
The SpyBLI cell-free pipeline for the rapid quantification of binding kinetics from crude samples Open
A less than 24-hour workflow with little hands-on time delivers quantitative protein binding kinetic data straight from expression mixtures, bypassing both binder purification and concentration determination.
View article: A platform for SpyCatcher conjugation to native antibodies
A platform for SpyCatcher conjugation to native antibodies Open
We introduce a platform for the conjugation of SpyCatcher proteins to native antibodies using cysteine-reactive tetra-divinylpyrimidine (TetraDVP)-SpyTag linkers.
View article: Digitally immune optimised haemagglutinin with nanocage plug-and-display elicits broadly neutralising pan-H5 influenza subtype vaccine responses
Digitally immune optimised haemagglutinin with nanocage plug-and-display elicits broadly neutralising pan-H5 influenza subtype vaccine responses Open
The increasing global spread of the highly pathogenic avian influenza (HPAI) A/H5 viruses poses a serious public health threat. Circulating clade 2.3.4.4b viruses have demonstrated rapid transcontinental dissemination, extensive reassortme…
View article: Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development
Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development Open
Influenza virus neuraminidase is a crucial target for protective antibodies, yet the development of recombinant neuraminidase protein as a vaccine has been held back by instability and variable expression. We have taken a pragmatic approac…
View article: <i>iMAX</i> FRET (Information Maximized FRET) for Multipoint Single-Molecule Structural Analysis
<i>iMAX</i> FRET (Information Maximized FRET) for Multipoint Single-Molecule Structural Analysis Open
Understanding the structure of biomolecules is vital for deciphering their roles in biological systems. Single-molecule techniques have emerged as alternatives to conventional ensemble structure analysis methods for uncovering new biology …
View article: Preclinical development of a stabilized RH5 virus-like particle vaccine that induces improved antimalarial antibodies
Preclinical development of a stabilized RH5 virus-like particle vaccine that induces improved antimalarial antibodies Open
Plasmodium falciparum reticulocyte-binding protein homolog 5 (RH5) is a leading blood-stage malaria vaccine antigen target, currently in a phase 2b clinical trial as a full-length soluble protein/adjuvant vaccine candidate called RH5.1/Mat…
View article: Proactive vaccination using multiviral Quartet Nanocages to elicit broad anti-coronavirus responses
Proactive vaccination using multiviral Quartet Nanocages to elicit broad anti-coronavirus responses Open
Defending against future pandemics requires vaccine platforms that protect across a range of related pathogens. Nanoscale patterning can be used to address this issue. Here, we produce quartets of linked receptor-binding domains (RBDs) fro…
View article: SpyMask enables combinatorial assembly of bispecific binders
SpyMask enables combinatorial assembly of bispecific binders Open
View article: Preclinical Development of a Stabilized RH5 Virus-Like Particle Vaccine that Induces Improved Anti-Malarial Antibodies
Preclinical Development of a Stabilized RH5 Virus-Like Particle Vaccine that Induces Improved Anti-Malarial Antibodies Open
The development of a highly effective vaccine against the pathogenic blood-stage infection of human malaria will require a delivery platform that can induce an antibody response of both maximal quantity and functional quality. One strategy…
View article: Visible Light-Induced Specific Protein Reaction Delineates Early Stages of Cell Adhesion
Visible Light-Induced Specific Protein Reaction Delineates Early Stages of Cell Adhesion Open
Light is well-established for control of bond breakage but not for control of specific bond formation in complex environments. We previously engineered the diffusion-limited reactivity of the SpyTag003 peptide with its protein partner SpyC…
View article: <i>iMAX</i>FRET (Information Maximized FRET) for multipoint single-molecule structural analysis
<i>iMAX</i>FRET (Information Maximized FRET) for multipoint single-molecule structural analysis Open
Understanding the structure of biomolecules is vital for deciphering their characteristics and roles in biological systems. While current structural analysis techniques like nuclear magnetic resonance and X-ray crystallography excel in man…
View article: SpyMask Enables Combinatorial Assembly of Bispecific Binders
SpyMask Enables Combinatorial Assembly of Bispecific Binders Open
Bispecific antibodies are a successful and expanding therapeutic class, bridging two cell-types or engaging two different molecules on the same cell. Bispecifics unlock avenues towards synergy, resistance evasion, and signaling bias. Stand…
View article: Visible light-induced specific protein reaction delineates early stages of cell adhesion
Visible light-induced specific protein reaction delineates early stages of cell adhesion Open
Light is well established for control of bond breakage, but not for control of specific bond formation in complex environments. We previously engineered diffusion-limited reactivity of SpyTag003 peptide with its protein partner SpyCatcher0…
View article: Design and Evolution of Enhanced Peptide–Peptide Ligation for Modular Transglutaminase Assembly
Design and Evolution of Enhanced Peptide–Peptide Ligation for Modular Transglutaminase Assembly Open
Robust and precise tools are needed to enhance the functionality and resilience of synthetic nanoarchitectures. Here, we have employed directed evolution and rational design to build a fast-acting molecular superglue from a bacterial adhes…
View article: Supplementary Figures 4-7 from Type 1 Insulin-like Growth Factor Receptor Translocates to the Nucleus of Human Tumor Cells
Supplementary Figures 4-7 from Type 1 Insulin-like Growth Factor Receptor Translocates to the Nucleus of Human Tumor Cells Open
Supplementary Figures 4-7 from Type 1 Insulin-like Growth Factor Receptor Translocates to the Nucleus of Human Tumor Cells
View article: Supplementary Materials, Table 1-2, Figures 1-3 from Type 1 Insulin-like Growth Factor Receptor Translocates to the Nucleus of Human Tumor Cells
Supplementary Materials, Table 1-2, Figures 1-3 from Type 1 Insulin-like Growth Factor Receptor Translocates to the Nucleus of Human Tumor Cells Open
Supplementary Materials, Table 1-2, Figures 1-3 from Type 1 Insulin-like Growth Factor Receptor Translocates to the Nucleus of Human Tumor Cells
View article: Supplementary Methods from Cholesterol Loading and Ultrastable Protein Interactions Determine the Level of Tumor Marker Required for Optimal Isolation of Cancer Cells
Supplementary Methods from Cholesterol Loading and Ultrastable Protein Interactions Determine the Level of Tumor Marker Required for Optimal Isolation of Cancer Cells Open
Supplementary Methods PDF file - 192K,Contains methods for cloning and expression of proteins, flow cytometry, biotinylation, microscopy, statistics and selected magnetic isolation procedures
View article: Data from Type 1 Insulin-like Growth Factor Receptor Translocates to the Nucleus of Human Tumor Cells
Data from Type 1 Insulin-like Growth Factor Receptor Translocates to the Nucleus of Human Tumor Cells Open
The type 1 insulin-like growth factor receptor (IGF-1R) is a transmembrane glycoprotein composed of two extracellular α subunits and two β subunits with tyrosine kinase activity. The IGF-1R is frequently upregulated in cancers and signals …