A. Mark Ibekwe
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View article: Agriculturally Sourced Multidrug-Resistant Escherichia coli for Use as Control Strains
Agriculturally Sourced Multidrug-Resistant Escherichia coli for Use as Control Strains Open
Bacteriological control strains with known characteristics ensure consistency and reproducibility of assay performance across different laboratories and are an important cornerstone of quality control in the microbiology laboratory. Ideall…
View article: Resistome Profile of Treated Wastewater Using Metagenomic Approach
Resistome Profile of Treated Wastewater Using Metagenomic Approach Open
The occurrence and dissemination of resistomes within environmental compartments are worrisome, considering the huge public health challenge they may pose. Treated wastewater from wastewater treatment plants (WWTPs) has been shown to conta…
View article: Agriculturally sourced multidrug-resistant <i>Escherichia coli</i> for use as control strains
Agriculturally sourced multidrug-resistant <i>Escherichia coli</i> for use as control strains Open
The environment plays a crucial role in the spread of antibiotic resistance, however environmental surveillance efforts lag far behind clinical surveillance. Natural and human- impacted soil and water are considered major sources of antibi…
View article: Quantitative Controls for Environmental and Agricultural Surveillance of Extended-Spectrum Beta-Lactamase Producing Escherichia coli v1
Quantitative Controls for Environmental and Agricultural Surveillance of Extended-Spectrum Beta-Lactamase Producing Escherichia coli v1 Open
The importance of standardized control strains for environmental antibiotic resistance (AR) efforts cannot be underestimated. Control strains are useful in standardizing the quality of testing across laboratories and are essential for qual…
View article: Unveiling errors in soil microbial community sequencing: a case for reference soils and improved diagnostics for nanopore sequencing
Unveiling errors in soil microbial community sequencing: a case for reference soils and improved diagnostics for nanopore sequencing Open
The sequencing platform and workflow strongly influence microbial community analyses through potential errors at each step. Effective diagnostics and experimental controls are needed to validate data and improve reproducibility. This cross…
View article: A one health approach for monitoring antimicrobial resistance: developing a national freshwater pilot effort
A one health approach for monitoring antimicrobial resistance: developing a national freshwater pilot effort Open
Antimicrobial resistance (AMR) is a world-wide public health threat that is projected to lead to 10 million annual deaths globally by 2050. The AMR public health issue has led to the development of action plans to combat AMR, including imp…
View article: An analysis of culture-based methods used for the detection and isolation of Salmonella spp., Escherichia coli, and Enterococcus spp. from surface water: A systematic review
An analysis of culture-based methods used for the detection and isolation of Salmonella spp., Escherichia coli, and Enterococcus spp. from surface water: A systematic review Open
Identification of methods for the standardized assessment of bacterial pathogens and antimicrobial resistance (AMR) in environmental water can improve the quality of monitoring and data collected, support global surveillance efforts, and e…
View article: Enterococcus Species and Their Antimicrobial Resistance in an Urban Watershed Affected by Different Anthropogenic Sources
Enterococcus Species and Their Antimicrobial Resistance in an Urban Watershed Affected by Different Anthropogenic Sources Open
Different anthropogenic sources can have a significant influence on bacterial populations and their antimicrobial activities. In this study, the impact of anthropogenic activities on Enterococcus species was studied in an urban watershed i…
View article: Dissemination of antibiotic resistance genes through soil-plant-earthworm continuum in the food production environment
Dissemination of antibiotic resistance genes through soil-plant-earthworm continuum in the food production environment Open
Treated municipal wastewater (TMW) can provide a reliable source of irrigation water for crops, which is especially important in arid areas where water resources are limited or prone to drought. Nonetheless, TMW may contain residual antibi…
View article: A comparison of methods to detect low levels of Salmonella enterica in surface waters to support antimicrobial resistance surveillance efforts performed in multiple laboratories
A comparison of methods to detect low levels of Salmonella enterica in surface waters to support antimicrobial resistance surveillance efforts performed in multiple laboratories Open
Developing effective and sensitive detection methods for antimicrobial resistant Salmonella enterica from surface water is a goal of the National Antimicrobial Resistance Monitoring System (NARMS). There are no specified methods for recove…
View article: Persistence of E. coli O157:H7 in Frozen Soils: Role of Freezing Temperature
Persistence of E. coli O157:H7 in Frozen Soils: Role of Freezing Temperature Open
Soilborne pathogen infections are increasingly reported globally in recent years. Infectious agents have contaminated most of seasonal frozen zone and have been found in permafrost due to the effects of intensified human activities on glob…
View article: Editorial: Exposure, Risks, and Drivers of the Mobile Antimicrobial Resistance Genes in the Environment—a Global Perspective
Editorial: Exposure, Risks, and Drivers of the Mobile Antimicrobial Resistance Genes in the Environment—a Global Perspective Open
EDITORIAL article Front. Microbiol., 30 November 2021Sec. Antimicrobials, Resistance and Chemotherapy Volume 12 - 2021 | https://doi.org/10.3389/fmicb.2021.803282
View article: Prevalence of Antibiotic Resistance Genes in Pharmaceutical Wastewaters
Prevalence of Antibiotic Resistance Genes in Pharmaceutical Wastewaters Open
Pharmaceutical wastewaters are recognized as reservoirs of antibiotic resistance genes (ARGs) and antibiotic resistant bacteria (ARB), and also as hotspots for their horizontal gene transfer (HGT) using mobile genetic elements. Our study e…
View article: Influence of seasonal changes and salinity on spinach phyllosphere bacterial functional assemblage
Influence of seasonal changes and salinity on spinach phyllosphere bacterial functional assemblage Open
The phyllosphere is the aerial part of plants that is exposed to different environmental conditions and is also known to harbor a wide variety of bacteria including both plant and human pathogens. However, studies on phyllosphere bacterial…
View article: Diversity of Plasmids and Genes Encoding Resistance to Extended-Spectrum β-Lactamase in Escherichia coli from Different Animal Sources
Diversity of Plasmids and Genes Encoding Resistance to Extended-Spectrum β-Lactamase in Escherichia coli from Different Animal Sources Open
Antimicrobial resistance associated with the spread of plasmid-encoded extended-spectrum β-lactamase (ESBL) genes conferring resistance to third generation cephalosporins is increasing worldwide. However, data on the population of ESBL pro…
View article: Antibiotic Resistance Genes Occurrence in Wastewaters from Selected Pharmaceutical Facilities in Nigeria
Antibiotic Resistance Genes Occurrence in Wastewaters from Selected Pharmaceutical Facilities in Nigeria Open
The proliferation of antibiotic-resistant bacteria (ARB) and the prevalence of antibiotic resistance genes (ARGs) in wastewaters are well-established factors that contribute to the reduced potency of antibiotics used in healthcare worldwid…
View article: Tricarboxylic Acid (TCA) Cycle Enzymes and Intermediates Modulate Intracellular Cyclic di-GMP Levels and the Production of Plant Cell Wall–Degrading Enzymes in Soft Rot Pathogen<i>Dickeya dadantii</i>
Tricarboxylic Acid (TCA) Cycle Enzymes and Intermediates Modulate Intracellular Cyclic di-GMP Levels and the Production of Plant Cell Wall–Degrading Enzymes in Soft Rot Pathogen<i>Dickeya dadantii</i> Open
Dickeya dadantii is a plant-pathogenic bacterium that causes soft-rot in a wide range of plants. Although we have previously demonstrated that cyclic bis-(3′-5′)-cyclic dimeric guanosine monophosphate (c-di-GMP), a bacterial secondary mess…
View article: Linking Microbial Community Composition in Treated Wastewater with Water Quality in Distribution Systems and Subsequent Health Effects
Linking Microbial Community Composition in Treated Wastewater with Water Quality in Distribution Systems and Subsequent Health Effects Open
The increases in per capita water consumption, coupled in part with global climate change have resulted in increased demands on available freshwater resources. Therefore, the availability of safe, pathogen-free drinking water is vital to p…
View article: A feed‐forward signalling circuit controls bacterial virulence through linking cyclic di‐GMP and two mechanistically distinct sRNAs, ArcZ and RsmB
A feed‐forward signalling circuit controls bacterial virulence through linking cyclic di‐GMP and two mechanistically distinct sRNAs, ArcZ and RsmB Open
Summary Dickeya dadantii is a plant pathogen that causes soft rot disease on vegetable and potato crops. To successfully cause infection, this pathogen needs to coordinately modulate the expression of genes encoding several virulence deter…
View article: Continuous Flow-Constructed Wetlands for the Treatment of Swine Waste Water
Continuous Flow-Constructed Wetlands for the Treatment of Swine Waste Water Open
The microbiological quality of treated waste water is always a concern when waste water is disposed to the environment. However, when treated appropriately, such water can serve many purposes to the general population. Therefore, the treat…
View article: Molecular Methods for Assessment of Antibiotic Resistance in Agricultural Ecosystems: Prospects and Challenges
Molecular Methods for Assessment of Antibiotic Resistance in Agricultural Ecosystems: Prospects and Challenges Open
Agricultural ecosystems are of special interest for monitoring the potential for antibiotic resistance to spread through the environment and contribute to human exposure. Molecular methods, which target DNA, RNA, and other molecular compon…
View article: Potential pathogens, antimicrobial patterns and genotypic diversity of<i>Escherichia coli</i>isolates in constructed wetlands treating swine wastewater
Potential pathogens, antimicrobial patterns and genotypic diversity of<i>Escherichia coli</i>isolates in constructed wetlands treating swine wastewater Open
Escherichia coli populations originating from swine houses through constructed wetlands were analyzed for potential pathogens, antimicrobial susceptibility patterns, and genotypic diversity. Escherichia coli isolates (n = 493) were screene…
View article: Cucumber Rhizosphere Microbial Community Response to Biocontrol Agent Bacillus subtilis B068150
Cucumber Rhizosphere Microbial Community Response to Biocontrol Agent Bacillus subtilis B068150 Open
Gram-positive bacteria Bacillus subtilis B068150 has been used as a biocontrol agent against the pathogen Fusarium oxysporum cucumerinum. Cucumber was grown in three soils with strain B068150 inoculated in a greenhouse for 90 days, and the…
View article: Prevalence and antimicrobial susceptibility of pathogenic Escherichia coli O157 in fresh produce obtained from irrigated fields
Prevalence and antimicrobial susceptibility of pathogenic Escherichia coli O157 in fresh produce obtained from irrigated fields Open
Escherichia coli O157 has been implicated in many outbreaks of gastroenteritis associated with the consumption of contaminated fresh vegetables, fruits and sprouts. In Nigeria, the use of untreated wastewater in irrigation is largely consi…