Mark J. Blacket
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View article: An annotated checklist of the psyllids (Hemiptera, Psylloidea) of Norfolk Island with keys to species, new records, and descriptions of two new endemic species
An annotated checklist of the psyllids (Hemiptera, Psylloidea) of Norfolk Island with keys to species, new records, and descriptions of two new endemic species Open
Norfolk Island is a small, isolated archipelago in the Pacific Ocean, 1400 km east of the Australian mainland. The history of human colonisation and land use on the island has resulted in a substantial reduction in the extent and quality o…
View article: Field assessment of current and improved surveillance traps for fruit flies (Diptera: Tephritidae) in Australia
Field assessment of current and improved surveillance traps for fruit flies (Diptera: Tephritidae) in Australia Open
Exotic fruit fly (Diptera: Tephritidae) surveillance in Australia predominantly relies on male-lure trapping. We assessed the performance of 3 traps currently used in Australian fruit fly surveillance: Lynfield, Modified Steiner, and Paton…
View article: Bridging the Gap Between Platforms: Comparing Grape Phylloxera Daktulosphaira vitifoliae (Fitch) Microsatellite Allele Size and DNA Sequence Variation
Bridging the Gap Between Platforms: Comparing Grape Phylloxera Daktulosphaira vitifoliae (Fitch) Microsatellite Allele Size and DNA Sequence Variation Open
Grape phylloxera, Daktulosphaira vitifoliae (Fitch), is an economically significant pest of grapevines. Identification of phylloxera genotypes is an important aspect of management as genotypes differ in virulence and susceptibility to cont…
View article: A morphological and high throughput sequencing workflow to identify Australian ants (Hymenoptera, Formicidae): a new tool for biosecurity and biodiversity
A morphological and high throughput sequencing workflow to identify Australian ants (Hymenoptera, Formicidae): a new tool for biosecurity and biodiversity Open
Ants are often the most ubiquitous and ecologically influential components of terrestrial systems, exhibiting an exceptionally high level of endemic diversity. Simultaneously, exotic ants can be amongst the most environmentally and economi…
View article: Diversity and composition of the bacterial communities associated with the Australian spittlebugs Bathyllus albicinctus and Philagra parva (Hemiptera: Aphrophoridae)
Diversity and composition of the bacterial communities associated with the Australian spittlebugs Bathyllus albicinctus and Philagra parva (Hemiptera: Aphrophoridae) Open
Spittlebugs and froghoppers (Hemiptera: Cercopoidea) are insects feeding on xylem, which potentially can cause significant economic damage worldwide by transmitting plant pathogenic bacteria such as Xylella fastidiosa . Australia and New Z…
View article: Effect of temperature and humidity on insect DNA integrity evaluated by real-time PCR
Effect of temperature and humidity on insect DNA integrity evaluated by real-time PCR Open
Insects collected in dry traps can degrade rapidly, especially in warm, humid environments where many biodiversity and biosecurity surveillance activities are undertaken. Degradation can severely impact diagnostics, as trap catches can bec…
View article: A novel diagnostic gene region for distinguishing between two pest fruit flies: <i>Bactrocera tryoni</i> (Froggatt) and <i>Bactrocera neohumeralis</i> (Hardy) (Diptera: Tephritidae)
A novel diagnostic gene region for distinguishing between two pest fruit flies: <i>Bactrocera tryoni</i> (Froggatt) and <i>Bactrocera neohumeralis</i> (Hardy) (Diptera: Tephritidae) Open
Bactrocera tryoni and Bactrocera neohumeralis are morphologically similar sibling pest fruit fly species that possess different biological attributes, geographic distributions, and host ranges. The need to differentiate between the two spe…
View article: Non-destructive insect metabarcoding for surveillance and biosecurity in citrus orchards: recording the good, the bad and the psyllids
Non-destructive insect metabarcoding for surveillance and biosecurity in citrus orchards: recording the good, the bad and the psyllids Open
Background The Australian citrus industry remains one of the few in the world to be unaffected by the African and the Asian citrus psyllids, Trioza erytreae Del Guercio and Diaphorina citri Kuwayama, respectively, and the diseases their ve…
View article: LAMP (Loop-mediated isothermal amplification) assay for rapid identification of Varroa mites
LAMP (Loop-mediated isothermal amplification) assay for rapid identification of Varroa mites Open
Varroa mites are serious pests of European honeybees ( Apis mellifera). For detection of Varroa mite, a new molecular LAMP-based assay has been developed, which retains the body of the mite intact for morphological identification. Six nove…
View article: LAMP assay for the detection of the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psylloidea: Psyllidae)
LAMP assay for the detection of the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psylloidea: Psyllidae) Open
Diaphorina citri Kuwayama, also known as the Asian citrus psyllid (ACP), can vector the bacterium Candidatus Liberibacter asiaticus (CLas), agent of Huanglongbing (HLB): an incurable disease affecting citrus trees worldwide. In citrus grow…
View article: The pest sap beetle Carpophilus (Myothorax) truncatus Murray, 1864 (Coleoptera: Nitidulidae)—a new synonymy and a related new species of Carpophilus
The pest sap beetle Carpophilus (Myothorax) truncatus Murray, 1864 (Coleoptera: Nitidulidae)—a new synonymy and a related new species of Carpophilus Open
Carpophilus truncatus Murray 1864, is a species of sap beetle which has been recorded from many countries worldwide, and has become recognised as an important pest of nuts. In this study, we present a re-description of C. truncatus includi…
View article: Development of a cost-effective, morphology-preserving method for DNA isolation from bulk invertebrate trap catches: Tephritid fruit flies as an exemplar
Development of a cost-effective, morphology-preserving method for DNA isolation from bulk invertebrate trap catches: Tephritid fruit flies as an exemplar Open
Insect identification and preservation of voucher specimens is integral to pest diagnostic and surveillance activities; yet bulk-trapped insects are a diagnostic challenge due to high catch numbers and the susceptibility of samples to envi…
View article: Non-destructive insect metabarcoding as a surveillance tool for the Australian grains industry: a first trial for the iMapPESTS smart trap
Non-destructive insect metabarcoding as a surveillance tool for the Australian grains industry: a first trial for the iMapPESTS smart trap Open
Surveillance and long-term monitoring of insect pest populations are of paramount importance to limit dispersal and inform pest management. Molecular methods have been employed in diagnostics, surveillance and monitoring for the past few d…
View article: The pest sap beetle Carpophilus Myothorax truncatus Murray, 1864 (Coleoptera Nitidulidae) - a new synonymy and a related new species of Carpophilus
The pest sap beetle Carpophilus Myothorax truncatus Murray, 1864 (Coleoptera Nitidulidae) - a new synonymy and a related new species of Carpophilus Open
This dataset contains the digitized treatments in Plazi based on the original journal article Semeraro, Linda, Blacket, Mark J., Rako, Lea, Cunningham, John Paul (2023): The pest sap beetle Carpophilus Myothorax truncatus Murray, 1864 (Col…
View article: DNA Metabarcoding Enables High-Throughput Detection of Spotted Wing Drosophila (Drosophila suzukii) Within Unsorted Trap Catches
DNA Metabarcoding Enables High-Throughput Detection of Spotted Wing Drosophila (Drosophila suzukii) Within Unsorted Trap Catches Open
The spotted wing drosophila ( Drosophila suzukii , Matsumara) is a rapidly spreading global pest of soft and stone fruit production. Due to the similarity of many of its life stages to other cosmopolitan drosophilids, surveillance for this…
View article: Peer Review #1 of "Disentangling bias for non-destructive insect metabarcoding (v0.1)"
Peer Review #1 of "Disentangling bias for non-destructive insect metabarcoding (v0.1)" Open
A fast and reliable method for obtaining a species-level identification is a fundamental requirement for a wide range of activities, from plant protection and invasive species management to biodiversity assessments and ecological studies.F…
View article: Disentangling bias for non-destructive insect metabarcoding
Disentangling bias for non-destructive insect metabarcoding Open
A fast and reliable method for obtaining a species-level identification is a fundamental requirement for a wide range of activities, from plant protection and invasive species management to biodiversity assessments and ecological studies. …
View article: A diagnostic LAMP assay for rapid identification of an invasive plant pest, fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae)
A diagnostic LAMP assay for rapid identification of an invasive plant pest, fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae) Open
Fall armyworm (FAW), Spodoptera frugiperda (Lepidoptera: Noctuidae), is a highly polyphagous invasive plant pest that has expanded its global geographic distribution, including recently into much of Australia. Rapid diagnostic tests are re…
View article: Description of an Australian endemic species of Trioza (Hemiptera: Triozidae) pest of the endemic tea tree, Melaleuca alternifolia (Myrtaceae)
Description of an Australian endemic species of Trioza (Hemiptera: Triozidae) pest of the endemic tea tree, Melaleuca alternifolia (Myrtaceae) Open
Psyllids, also known as jumping plant lice, are phloem feeding Hemiptera that often show a strict species-specific relationship with their host plants. When psyllid-plant associations involve economically important crops, this may lead to …
View article: A <scp>LAMP</scp> (loop‐mediated isothermal amplification) test for rapid identification of Khapra beetle (<scp><i>Trogoderma granarium</i></scp>)
A <span>LAMP</span> (loop‐mediated isothermal amplification) test for rapid identification of Khapra beetle (<span><i>Trogoderma granarium</i></span>) Open
BACKGROUND Khapra beetle ( Trogoderma granarium Everts) is a significant pest of food products around the world, causing great losses of stored grain and produce, with export restrictions imposed on countries with established beetle popula…
View article: Developing a non-destructive metabarcoding protocol for detection of pest insects in bulk trap catches
Developing a non-destructive metabarcoding protocol for detection of pest insects in bulk trap catches Open
Metabarcoding has the potential to revolutionise insect surveillance by providing high-throughput and cost-effective species identification of all specimens within mixed trap catches. Nevertheless, incorporation of metabarcoding into insec…
View article: Computational Evaluation of DNA Metabarcoding for Universal Diagnostics of Invasive Insect Pests
Computational Evaluation of DNA Metabarcoding for Universal Diagnostics of Invasive Insect Pests Open
Appropriate design and selection of PCR primers plays a critical role in determining the sensitivity and specificity of a metabarcoding assay. Despite several studies applying metabarcoding to insect pest surveillance, the diagnostic perfo…
View article: Propylene Glycol and Non-Destructive DNA Extractions Enable Preservation and Isolation of Insect and Hosted Bacterial DNA
Propylene Glycol and Non-Destructive DNA Extractions Enable Preservation and Isolation of Insect and Hosted Bacterial DNA Open
Plant bio-protection and biosecurity programs worldwide use trap-based surveillance for the early detection of agricultural pests and pathogens to contain their incursions and spread. This task is reliant on effective preservation in insec…
View article: Developing a Non-destructive Metabarcoding Protocol for Detection of Pest Insects in Bulk Trap Catches
Developing a Non-destructive Metabarcoding Protocol for Detection of Pest Insects in Bulk Trap Catches Open
Metabarcoding has the potential to revolutionise insect surveillance by providing high-throughput and cost-effective species identification of all specimens within mixed trap catches. Nevertheless, incorporation of metabarcoding into insec…
View article: A LAMP assay for the detection of Bactrocera tryoni Queensland fruit fly (Diptera: Tephritidae)
A LAMP assay for the detection of Bactrocera tryoni Queensland fruit fly (Diptera: Tephritidae) Open
LAMP assays are targeted molecular tests for the rapid detection of species in the laboratory and field. We developed a LAMP assay for an economically important fruit fly species, Queensland fruit fly, Bactrocera tryoni . This assay was as…
View article: Illuminating Insights into the Biodiversity of the Australian Psyllids (Hemiptera: Psylloidea) Collected Using Light Trapping
Illuminating Insights into the Biodiversity of the Australian Psyllids (Hemiptera: Psylloidea) Collected Using Light Trapping Open
The superfamily Psylloidea includes numerous species which play a key role in Australian ecology and biodiversity, as well as pests and biological control agents, and sometimes threatened species of conservation concern. Different psyllid …
View article: On the complementarity of DNA barcoding and morphology to distinguish benign endemic insects from possible pests: the case of <i>Dirioxa pornia</i> and the tribe Acanthonevrini (Diptera: Tephritidae: Phytalmiinae) in Australia
On the complementarity of DNA barcoding and morphology to distinguish benign endemic insects from possible pests: the case of <i>Dirioxa pornia</i> and the tribe Acanthonevrini (Diptera: Tephritidae: Phytalmiinae) in Australia Open
Fruit flies are considered economically important insects due to some species being agricultural pests. However, morphological identification of fruit fly adults and larvae can be difficult requiring a high level of taxonomic expertise, wi…
View article: Developing a non-destructive metabarcoding protocol for detection of pest insects in bulk trap catches
Developing a non-destructive metabarcoding protocol for detection of pest insects in bulk trap catches Open
Supporting information for the manuscript "Developing a non-destructive metabarcoding protocol for detection of pest insects in bulk trap catches" by J. Batovska, A.M. Piper, I. Valenzuela, J.P. Cunningham & M.J. Blacket