Mark S. Dunstan
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View article: AutoZooMS: Integrating robotics into high-throughput ZooMS for the species identification of palaeontological remains at Grotte Mandrin, France
AutoZooMS: Integrating robotics into high-throughput ZooMS for the species identification of palaeontological remains at Grotte Mandrin, France Open
View article: AutoZooMS: Integrating robotics into high-throughput ZooMS for the species identification of archaeofaunal remains at Grotte Mandrin, France
AutoZooMS: Integrating robotics into high-throughput ZooMS for the species identification of archaeofaunal remains at Grotte Mandrin, France Open
As the vast majority of excavated archaeofaunal skeletal remains are fragmentary to the extent that they cannot be identified by morphological analysis alone, various molecular methods have been considered to retrieve information from an o…
View article: Supplementary Figures 1 - 6 from <i>In Silico</i> Screening Reveals Structurally Diverse, Nanomolar Inhibitors of NQO2 That Are Functionally Active in Cells and Can Modulate NF-κB Signaling
Supplementary Figures 1 - 6 from <i>In Silico</i> Screening Reveals Structurally Diverse, Nanomolar Inhibitors of NQO2 That Are Functionally Active in Cells and Can Modulate NF-κB Signaling Open
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View article: Supplementary Tables 1 - 2 from <i>In Silico</i> Screening Reveals Structurally Diverse, Nanomolar Inhibitors of NQO2 That Are Functionally Active in Cells and Can Modulate NF-κB Signaling
Supplementary Tables 1 - 2 from <i>In Silico</i> Screening Reveals Structurally Diverse, Nanomolar Inhibitors of NQO2 That Are Functionally Active in Cells and Can Modulate NF-κB Signaling Open
PDF file - 162KB
View article: Data from <i>In Silico</i> Screening Reveals Structurally Diverse, Nanomolar Inhibitors of NQO2 That Are Functionally Active in Cells and Can Modulate NF-κB Signaling
Data from <i>In Silico</i> Screening Reveals Structurally Diverse, Nanomolar Inhibitors of NQO2 That Are Functionally Active in Cells and Can Modulate NF-κB Signaling Open
The National Cancer Institute chemical database has been screened using in silico docking to identify novel nanomolar inhibitors of NRH:quinone oxidoreductase 2 (NQO2). The inhibitors identified from the screen exhibit a diverse ran…
View article: Supplementary Figures 1 - 6 from <i>In Silico</i> Screening Reveals Structurally Diverse, Nanomolar Inhibitors of NQO2 That Are Functionally Active in Cells and Can Modulate NF-κB Signaling
Supplementary Figures 1 - 6 from <i>In Silico</i> Screening Reveals Structurally Diverse, Nanomolar Inhibitors of NQO2 That Are Functionally Active in Cells and Can Modulate NF-κB Signaling Open
PDF file - 626KB
View article: Supplementary Tables 1 - 2 from <i>In Silico</i> Screening Reveals Structurally Diverse, Nanomolar Inhibitors of NQO2 That Are Functionally Active in Cells and Can Modulate NF-κB Signaling
Supplementary Tables 1 - 2 from <i>In Silico</i> Screening Reveals Structurally Diverse, Nanomolar Inhibitors of NQO2 That Are Functionally Active in Cells and Can Modulate NF-κB Signaling Open
PDF file - 162KB
View article: Data from <i>In Silico</i> Screening Reveals Structurally Diverse, Nanomolar Inhibitors of NQO2 That Are Functionally Active in Cells and Can Modulate NF-κB Signaling
Data from <i>In Silico</i> Screening Reveals Structurally Diverse, Nanomolar Inhibitors of NQO2 That Are Functionally Active in Cells and Can Modulate NF-κB Signaling Open
The National Cancer Institute chemical database has been screened using in silico docking to identify novel nanomolar inhibitors of NRH:quinone oxidoreductase 2 (NQO2). The inhibitors identified from the screen exhibit a diverse ran…
View article: Prototyping of microbial chassis for the biomanufacturing of high-value chemical targets
Prototyping of microbial chassis for the biomanufacturing of high-value chemical targets Open
Metabolic engineering technologies have been employed with increasing success over the last three decades for the engineering and optimization of industrial host strains to competitively produce high-value chemical targets. To this end, co…
View article: A plasmid toolset for CRISPR‐mediated genome editing and CRISPRi gene regulation in <i>Escherichia coli</i>
A plasmid toolset for CRISPR‐mediated genome editing and CRISPRi gene regulation in <i>Escherichia coli</i> Open
Summary CRISPR technologies have become standard laboratory tools for genetic manipulations across all kingdoms of life. Despite their origins in bacteria, the development of CRISPR tools for engineering bacteria has been slower than for e…
View article: Rapid prototyping of microbial production strains for the biomanufacture of potential materials monomers
Rapid prototyping of microbial production strains for the biomanufacture of potential materials monomers Open
View article: Engineering Escherichia coli towards de novo production of gatekeeper (2S)-flavanones: naringenin, pinocembrin, eriodictyol and homoeriodictyol
Engineering Escherichia coli towards de novo production of gatekeeper (2S)-flavanones: naringenin, pinocembrin, eriodictyol and homoeriodictyol Open
Natural plant-based flavonoids have drawn significant attention as dietary supplements due to their potential health benefits, including anti-cancer, anti-oxidant and anti-asthmatic activities. Naringenin, pinocembrin, eriodictyol and homo…
View article: Ribosomal Protein L11 Selectively Stabilizes a Tertiary Structure of the GTPase Center rRNA Domain
Ribosomal Protein L11 Selectively Stabilizes a Tertiary Structure of the GTPase Center rRNA Domain Open
View article: An automated pipeline for the screening of diverse monoterpene synthase libraries
An automated pipeline for the screening of diverse monoterpene synthase libraries Open
View article: SYNBIOCHEM - Combinatorial plasmid libraries for material monomer production in Escherichia coli.
SYNBIOCHEM - Combinatorial plasmid libraries for material monomer production in Escherichia coli. Open
Combinatorial plasmid libraries for material monomer production in Escherichia coli. - Design of Experiments: To investigate the production of material monomer targets in E. coli, combinatorial plasmid libraries (carrying genes for enzyme …
View article: SelProm: A Queryable and Predictive Expression Vector Selection Tool for <i>Escherichia coli</i>
SelProm: A Queryable and Predictive Expression Vector Selection Tool for <i>Escherichia coli</i> Open
The rapid prototyping and optimization of plasmid-based recombinant gene expression is one of the key steps in the development of bioengineered bacterial systems. Often, multiple genes or gene modules need to be coexpressed, and for this p…
View article: Highly multiplexed, fast and accurate nanopore sequencing for verification of synthetic DNA constructs and sequence libraries
Highly multiplexed, fast and accurate nanopore sequencing for verification of synthetic DNA constructs and sequence libraries Open
Synthetic biology utilizes the Design–Build–Test–Learn pipeline for the engineering of biological systems. Typically, this requires the construction of specifically designed, large and complex DNA assemblies. The availability of cheap DNA …
View article: Machine Learning of Designed Translational Control Allows Predictive Pathway Optimization in <i>Escherichia coli</i>
Machine Learning of Designed Translational Control Allows Predictive Pathway Optimization in <i>Escherichia coli</i> Open
The field of synthetic biology aims to make the design of biological systems predictable, shrinking the huge design space to practical numbers for testing. When designing microbial cell factories, most optimization efforts have focused on …
View article: An automated Design-Build-Test-Learn pipeline for enhanced microbial production of fine chemicals
An automated Design-Build-Test-Learn pipeline for enhanced microbial production of fine chemicals Open
View article: Structure and Biocatalytic Scope of Coclaurine <i>N</i>‐Methyltransferase
Structure and Biocatalytic Scope of Coclaurine <i>N</i>‐Methyltransferase Open
Benzylisoquinoline alkaloids (BIAs) are a structurally diverse family of plant secondary metabolites, which have been exploited to develop analgesics, antibiotics, antitumor agents, and other therapeutic agents. Biosynthesis of BIAs procee…
View article: Structure and Biocatalytic Scope of Coclaurine <i>N</i>‐Methyltransferase
Structure and Biocatalytic Scope of Coclaurine <i>N</i>‐Methyltransferase Open
Benzylisoquinoline alkaloids (BIAs) are a structurally diverse family of plant secondary metabolites, which have been exploited to develop analgesics, antibiotics, antitumor agents, and other therapeutic agents. Biosynthesis of BIAs procee…
View article: SYNBIOCHEM Design-Build-Test-Learn pipeline
SYNBIOCHEM Design-Build-Test-Learn pipeline Open
Quantified titers and peak values measured through different rounds of the SYNBIOCHEM Design-Build-Test-Learn (DBTL) pipeline for microbial production of fine chemicals. The pipeline was applied for the production of flavonoids and alkaloi…
View article: PartsGenie: an integrated tool for optimizing and sharing synthetic biology parts
PartsGenie: an integrated tool for optimizing and sharing synthetic biology parts Open
Motivation Synthetic biology is typified by developing novel genetic constructs from the assembly of reusable synthetic DNA parts, which contain one or more features such as promoters, ribosome binding sites, coding sequences and terminato…
View article: Engineering the “Missing Link” in Biosynthetic (−)-Menthol Production: Bacterial Isopulegone Isomerase
Engineering the “Missing Link” in Biosynthetic (−)-Menthol Production: Bacterial Isopulegone Isomerase Open
The realization of a synthetic biology approach to microbial (1R,2S,5R)-(-)-menthol (1) production relies on the identification of a gene encoding an isopulegone isomerase (IPGI), the only enzyme in the <…
View article: Zymophore identification enables the discovery of novel phenylalanine ammonia lyase enzymes
Zymophore identification enables the discovery of novel phenylalanine ammonia lyase enzymes Open
View article: Adenylation Activity of Carboxylic Acid Reductases Enables the Synthesis of Amides
Adenylation Activity of Carboxylic Acid Reductases Enables the Synthesis of Amides Open
Carboxylic acid reductases (CARs) catalyze the reduction of a broad range of carboxylic acids to aldehydes using the cofactors adenosine triphosphate and nicotinamide adenine dinucleotide phosphate, and have become attractive biocatalysts …
View article: PartsGenie: an integrated tool for optimising and sharing synthetic biology parts
PartsGenie: an integrated tool for optimising and sharing synthetic biology parts Open
Synthetic biology is typified by developing novel genetic constructs from the assembly of reusable synthetic DNA parts, which contain one or more features such as promoters, ribosome binding sites, coding sequences and terminators. While r…
View article: Structures of carboxylic acid reductase reveal domain dynamics underlying catalysis
Structures of carboxylic acid reductase reveal domain dynamics underlying catalysis Open
View article: biochem4j: Integrated and extensible biochemical knowledge through graph databases
biochem4j: Integrated and extensible biochemical knowledge through graph databases Open
Biologists and biochemists have at their disposal a number of excellent, publicly available data resources such as UniProt, KEGG, and NCBI Taxonomy, which catalogue biological entities. Despite the usefulness of these resources, they remai…
View article: SYNBIOCHEM Synthetic Biology Research Centre, Manchester – A UK foundry for fine and speciality chemicals production
SYNBIOCHEM Synthetic Biology Research Centre, Manchester – A UK foundry for fine and speciality chemicals production Open