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View article: Feasibility Study for the Use of Gene Electrotransfer and Cell Electrofusion as a Single-Step Technique for the Generation of Activated Cancer Cell Vaccines
Feasibility Study for the Use of Gene Electrotransfer and Cell Electrofusion as a Single-Step Technique for the Generation of Activated Cancer Cell Vaccines Open
Cell-based therapies hold great potential for cancer immunotherapy. This approach is based on manipulation of dendritic cells to activate immune system against specific cancer antigens. For the development of an effective cell vaccine plat…
View article: Error-rate reduction in network-based biocomputation
Error-rate reduction in network-based biocomputation Open
Network-based biocomputation (NBC) is an alternative parallel computing paradigm that encodes combinatorial problems into a nanofabricated device's graphical network of channels, enabling cytoskeletal filaments propelled by molecular motor…
View article: Cost-Efficient Expression of Human Cardiac Myosin Heavy Chain in C2C12 Cells with a Non-Viral Transfection Reagent
Cost-Efficient Expression of Human Cardiac Myosin Heavy Chain in C2C12 Cells with a Non-Viral Transfection Reagent Open
Production of functional myosin heavy chain (MHC) of striated muscle myosin II for studies of isolated proteins requires mature muscle (e.g., C2C12) cells for expression. This is important both for fundamental studies of molecular mechanis…
View article: Improved longevity of actomyosin in vitro motility assays for sustainable lab-on-a-chip applications
Improved longevity of actomyosin in vitro motility assays for sustainable lab-on-a-chip applications Open
In the in vitro motility assay (IVMA), actin filaments are observed while propelled by surface-adsorbed myosin motor fragments such as heavy meromyosin (HMM). In addition to fundamental studies, the IVMA is the basis for a range of lab-on-…
View article: Feasibility study for the use of gene electrotransfer and cell electrofusion as a single-step technique for the generation of activated cancer cell vaccines
Feasibility study for the use of gene electrotransfer and cell electrofusion as a single-step technique for the generation of activated cancer cell vaccines Open
In the search for safe induction of cellular and humoral cancer immunity dendritic cell-based therapies hold great potential. This approach is based on manipulation of dendritic cells to activate immune system against specific cancer antig…
View article: Probing actin‐activated <scp>ATP</scp> turnover kinetics of human cardiac myosin <scp>II</scp> by single molecule fluorescence
Probing actin‐activated <span>ATP</span> turnover kinetics of human cardiac myosin <span>II</span> by single molecule fluorescence Open
Mechanistic insights into myosin II energy transduction in striated muscle in health and disease would benefit from functional studies of a wide range of point‐mutants. This approach is, however, hampered by the slow turnaround of myosin I…
View article: Exploitation of Engineered Light-Switchable Myosin XI for Nanotechnological Applications
Exploitation of Engineered Light-Switchable Myosin XI for Nanotechnological Applications Open
For certain nanotechnological applications of the contractile proteins actin and myosin, e.g., in biosensing and network-based biocomputation, it would be desirable to temporarily switch on/off motile function in parts of nanostructured de…
View article: New paradigms in actomyosin energy transduction: Critical evaluation of non‐traditional models for orthophosphate release
New paradigms in actomyosin energy transduction: Critical evaluation of non‐traditional models for orthophosphate release Open
Release of the ATP hydrolysis product ortophosphate (Pi) from the active site of myosin is central in chemo‐mechanical energy transduction and closely associated with the main force‐generating structural change, the power‐stroke. Despite i…
View article: New paradigms in actomyosin energy transduction: critical evaluation of non-traditional models for orthophosphate release
New paradigms in actomyosin energy transduction: critical evaluation of non-traditional models for orthophosphate release Open
Release of the ATP hydrolysis product inorganic phosphate (Pi) from the active site of myosin is central in chemo-mechanical energy transduction and closely associated with the main force-generating structural change, the power-stroke. Des…
View article: Single molecule dataset for article: Multistep orthophosphate release tunes actomyosin energy transduction
Single molecule dataset for article: Multistep orthophosphate release tunes actomyosin energy transduction Open
Dataset (single molecule movies) that is behind the results in the article's Figure 2 and Figure 3. MATLAB scripts used to analyze the dataset.
View article: Single molecule dataset for article: Multistep orthophosphate release tunes actomyosin energy transduction
Single molecule dataset for article: Multistep orthophosphate release tunes actomyosin energy transduction Open
Dataset (single molecule movies) that is behind the results in the article's Figure 2 and Figure 3. MATLAB scripts used to analyze the dataset.
View article: Critical Evaluation of Current Hypotheses for the Pathogenesis of Hypertrophic Cardiomyopathy
Critical Evaluation of Current Hypotheses for the Pathogenesis of Hypertrophic Cardiomyopathy Open
Hereditary hypertrophic cardiomyopathy (HCM), due to mutations in sarcomere proteins, occurs in more than 1/500 individuals and is the leading cause of sudden cardiac death in young people. The clinical course exhibits appreciable variabil…
View article: Prolonged function and optimization of actomyosin motility for upscaled network-based biocomputation
Prolonged function and optimization of actomyosin motility for upscaled network-based biocomputation Open
Significant advancements have been made towards exploitation of naturally available molecular motors and their associated cytoskeletal filaments in nanotechnological applications. For instance, myosin motors and actin filaments from muscle…
View article: Single molecule turnover of fluorescent ATP by myosin and actomyosin unveil elusive enzymatic mechanisms
Single molecule turnover of fluorescent ATP by myosin and actomyosin unveil elusive enzymatic mechanisms Open
Benefits of single molecule studies of biomolecules include the need for minimal amounts of material and the potential to reveal phenomena hidden in ensembles. However, results from recent single molecule studies of fluorescent ATP turnove…
View article: Myosin and gelsolin cooperate in actin filament severing and actomyosin motor activity
Myosin and gelsolin cooperate in actin filament severing and actomyosin motor activity Open
Actin is a major intracellular protein with key functions in cellular motility, signaling, and structural rearrangements. Its dynamic behavior, such as polymerization and depolymerization of actin filaments in response to intracellular and…
View article: Myosin-gelsolin cooperativity in actin filament severing and actomyosin motor activity
Myosin-gelsolin cooperativity in actin filament severing and actomyosin motor activity Open
Actin is a major intracellular protein with key functions in cellular motility, signalling and structural rearrangements. Its dynamic behavior with actin filaments (F-actin) polymerising and depolymerising in response to intracellular chan…
View article: Optimized single molecule fluorescence sheds light on elusive enzymatic mechanisms
Optimized single molecule fluorescence sheds light on elusive enzymatic mechanisms Open
Single molecule enzymology using fluorescent substrate requires truly minimal amounts of proteins. This is highly beneficial when the protein source is either advanced expression systems or samples from humans/animals with ethical and econ…
View article: Blebbistatin Effects Expose Hidden Secrets in the Force-Generating Cycle of Actin and Myosin
Blebbistatin Effects Expose Hidden Secrets in the Force-Generating Cycle of Actin and Myosin Open
Cyclic interactions between myosin II motors and actin filaments driven by ATP turnover underlie muscle contraction and have key roles in the motility of nonmuscle cells. A remaining enigma in the understanding of this interaction is the r…
View article: Do Actomyosin Single-Molecule Mechanics Data Predict Mechanics of Contracting Muscle?
Do Actomyosin Single-Molecule Mechanics Data Predict Mechanics of Contracting Muscle? Open
In muscle, but not in single-molecule mechanics studies, actin, myosin and accessory proteins are incorporated into a highly ordered myofilament lattice. In view of this difference we compare results from single-molecule studies and muscle…