Markus Osterhoff
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View article: On super-resolution holography: effective geometry, sampling, and constraints
On super-resolution holography: effective geometry, sampling, and constraints Open
X-ray holo-tomography is a powerful tool for high-resolution three-dimensional imaging, but it faces inherent limitations in resolution caused by detector constraints, numerical aperture restrictions, and the empty beam division, which is …
View article: Interferometric measurement of nuclear resonant phase shift with a nanoscale Young double waveguide
Interferometric measurement of nuclear resonant phase shift with a nanoscale Young double waveguide Open
The phase shift of an electromagnetic wave, imprinted by its interaction with atomic scatterers, is a central quantity in optics and photonics. In particular, it encodes information about optical resonances and photon-matter interaction. W…
View article: Supplementary document for Super-Resolution X-ray Holography - 5226238.pdf
Supplementary document for Super-Resolution X-ray Holography - 5226238.pdf Open
Supplemental information
View article: X-ray phase-contrast tomography of cells manipulated with an optical stretcher
X-ray phase-contrast tomography of cells manipulated with an optical stretcher Open
X-rays can penetrate deeply into biological cells and thus allow for examination of their internal structures with high spatial resolution. In this study, X-ray phase-contrast imaging and tomography is combined with an X-ray-compatible opt…
View article: The collapse of a sonoluminescent cavitation bubble imaged with X-ray free-electron laser pulses
The collapse of a sonoluminescent cavitation bubble imaged with X-ray free-electron laser pulses Open
Single bubble sonoluminescence (SBSL) is the phenomenon of synchronous light emission due to the violent collapse of a single spherical bubble in a liquid, driven by an ultrasonic field. During the bubble collapse, matter inside the bubble…
View article: LiveDocs: Crafting Interactive Development Environments From Research Findings
LiveDocs: Crafting Interactive Development Environments From Research Findings Open
Open Science is a recurrent topic in scientific discussion, and there is a current effort to make research more accessible to a broader audience. A focus on delivering research findings that are reproducible, or even re-usable has been pro…
View article: Jetting bubbles observed by x-ray holography at a free-electron laser: internal structure and the effect of non-axisymmetric boundary conditions
Jetting bubbles observed by x-ray holography at a free-electron laser: internal structure and the effect of non-axisymmetric boundary conditions Open
In this work, we study the jetting dynamics of individual cavitation bubbles using x-ray holographic imaging and high-speed optical shadowgraphy. The bubbles are induced by a focused infrared laser pulse in water near the surface of a flat…
View article: Micropipette aspiration as a tool for single-particle X-ray imaging and diffraction
Micropipette aspiration as a tool for single-particle X-ray imaging and diffraction Open
A sample environment and manipulation tool is presented for single-particle X-ray experiments in an aqueous environment. The system is based on a single water droplet, positioned on a substrate that is structured by a hydrophobic and hydro…
View article: Structural dynamics of water in a supersonic shockwave
Structural dynamics of water in a supersonic shockwave Open
We explore the pressure evolution and structural dynamics of transient phase transitions in a microfluidic water jet after laser-induced dielectric breakdown. To this end, we use a combined approach of near-field holography with single fem…
View article: Combined optical fluorescence microscopy and X-ray tomography reveals substructures in cell nuclei in 3D
Combined optical fluorescence microscopy and X-ray tomography reveals substructures in cell nuclei in 3D Open
The function of a biological cell is fundamentally defined by the structural architecture of packaged DNA in the nucleus. Elucidating information about the packaged DNA is facilitated by high-resolution imaging. Here, we combine and correl…
View article: Coherent Diffractive Imaging with Diffractive Optics
Coherent Diffractive Imaging with Diffractive Optics Open
We present a novel approach to x-ray microscopy based on a multilayer zone plate which is positioned behind a sample similar to an objective lens. However, unlike transmission x-ray microscopy, we do not content ourselves with a sharp inte…
View article: Finite-difference propagation for the simulation of x-ray multilayer optics
Finite-difference propagation for the simulation of x-ray multilayer optics Open
Recent progress in nanofabrication, namely of multilayer optics, and the construction of coherent hard x-ray sources has enabled high resolution x-ray microscopy with large numerical aperture optics for small focal spot sizes. Sub-10 nm an…
View article: Super-resolution X-ray holography
Super-resolution X-ray holography Open
For non-destructive imaging of three-dimensional (3D) materials and biological specimen, hard X-ray in-line holography is particularly suitable, since it offers a phase-sensitive imaging scheme which can cover large specimen in a full-fiel…
View article: Off-axis multilayer zone plate with 16 nm × 28 nm focus for high-resolution X-ray beam induced current imaging
Off-axis multilayer zone plate with 16 nm × 28 nm focus for high-resolution X-ray beam induced current imaging Open
Using multilayer zone plates (MZPs) as two-dimensional optics, focal spot sizes of less than 10 nm can be achieved, as we show here with a focus of 8.4 nm × 9.6 nm, but the need for order-sorting apertures prohibits practical working dista…
View article: Pump-probe X-ray holographic imaging of laser-induced cavitation bubbles with femtosecond FEL pulses
Pump-probe X-ray holographic imaging of laser-induced cavitation bubbles with femtosecond FEL pulses Open
Cavitation bubbles can be seeded from a plasma following optical breakdown, by focusing an intense laser in water. The fast dynamics are associated with extreme states of gas and liquid, especially in the nascent state. This offers a uniqu…
View article: In-line holography with hard x-rays at sub-15 nm resolution
In-line holography with hard x-rays at sub-15 nm resolution Open
X-ray in-line holography is well suited for three-dimensional imaging, since it covers a large field of view without the necessity of scanning. However, its resolution does not extend to the range covered by coherent diffractive imaging or…
View article: Nanosecond timing and synchronization scheme for holographic pump–probe studies at the MID instrument at European XFEL
Nanosecond timing and synchronization scheme for holographic pump–probe studies at the MID instrument at European XFEL Open
Single-pulse holographic imaging at XFEL sources with 10 12 photons delivered in pulses shorter than 100 fs reveal new quantitative insights into fast phenomena. Here, a timing and synchronization scheme for stroboscopic imaging and quanti…
View article: Combined scanning small-angle X-ray scattering and holography probes multiple length scales in cell nuclei
Combined scanning small-angle X-ray scattering and holography probes multiple length scales in cell nuclei Open
X-rays are emerging as a complementary probe to visible-light photons and electrons for imaging biological cells. By exploiting their small wavelength and high penetration depth, it is possible to image whole, intact cells and resolve subc…
View article: Single-pulse phase-contrast imaging at free-electron lasers in the hard X-ray regime
Single-pulse phase-contrast imaging at free-electron lasers in the hard X-ray regime Open
X-ray free-electron lasers (XFELs) have opened up unprecedented opportunities for time-resolved nano-scale imaging with X-rays. Near-field propagation-based imaging, and in particular near-field holography (NFH) in its high-resolution impl…
View article: In Cellulo Analysis of Huntingtin Inclusion Bodies by Cryogenic Nanoprobe SAXS
In Cellulo Analysis of Huntingtin Inclusion Bodies by Cryogenic Nanoprobe SAXS Open
Huntington's disease (HD) is one of nine neurodegenerative disorders associated with an extension of polyglutamine (polyQ) in proteins. In HD, the polyQ tract in the huntingtin protein is extended beyond a threshold of 38 amino acids leadi…
View article: 3D virtual histology of human pancreatic tissue by multiscale phase-contrast X-ray tomography
3D virtual histology of human pancreatic tissue by multiscale phase-contrast X-ray tomography Open
A multiscale three-dimensional (3D) virtual histology approach is presented, based on two configurations of propagation phase-contrast X-ray tomography, which have been implemented in close proximity at the GINIX endstation at the beamline…
View article: 3D virtual pathohistology of lung tissue from Covid-19 patients based on phase contrast X-ray tomography
3D virtual pathohistology of lung tissue from Covid-19 patients based on phase contrast X-ray tomography Open
We present a three-dimensional (3D) approach for virtual histology and histopathology based on multi-scale phase contrast x-ray tomography, and use this to investigate the parenchymal architecture of unstained lung tissue from patients who…
View article: Author response: 3D virtual pathohistology of lung tissue from Covid-19 patients based on phase contrast X-ray tomography
Author response: 3D virtual pathohistology of lung tissue from Covid-19 patients based on phase contrast X-ray tomography Open
Article Figures and data Abstract Introduction Results Discussion Materials and methods Appendix 1 Appendix 2 Appendix 3 Data availability References Decision letter Author response Article and author information Metrics Abstract We presen…
View article: 3d Virtual Pathohistology of Lung Tissue from Covid-19 Patients based on Phase Contrast X-ray Tomography
3d Virtual Pathohistology of Lung Tissue from Covid-19 Patients based on Phase Contrast X-ray Tomography Open
We present a new approach of three-dimensional (3d) virtual histology and histopathology based on multi-scale phase contrast x-ray tomography, and use this to investigate the parenchymal architecture of unstained lung tissue from patients …
View article: Multilayer Zone Plates for Hard X-ray Imaging
Multilayer Zone Plates for Hard X-ray Imaging Open
This chapter reviews progress both in the fabrication of multilayer zone plate optics for focusing X-rays, as well as in imaging experiments using these optics. The fabrication based on pulsed laser deposition is accompanied by analytical …
View article: 3d Virtual Patho-Histology of Lung Tissue from Covid19 Patients based on Phase Contrast X-ray Tomography
3d Virtual Patho-Histology of Lung Tissue from Covid19 Patients based on Phase Contrast X-ray Tomography Open
X-ray phase contrast tomographys datasets of lung tissue from 6 Covid-19 patients (paraffin embedded unstained tissue), and one control tissue (healthy lung, in fixative solution). The data has been recorded as part of the Covid-19 Call fo…
View article: X-ray Focusing and Optics
X-ray Focusing and Optics Open
This chapter addresses fundamental concepts of X-ray optics and X-ray coherence, in view of the increasing number of X-ray applications requiring nano-focused X-ray beams. The chapter is meant as a tutorial to facilitate the understanding …
View article: 3d Virtual Patho-Histology of Lung Tissue from Covid19 Patients based on Phase Contrast X-ray Tomography
3d Virtual Patho-Histology of Lung Tissue from Covid19 Patients based on Phase Contrast X-ray Tomography Open
X-ray phase contrast tomographys datasets of lung tissue from 6 Covid-19 patients (paraffin embedded unstained tissue), and one control tissue (healthy lung, in fixative solution). The data has been recorded as part of the Covid-19 Call fo…
View article: Focus characterization of the NanoMAX Kirkpatrick–Baez mirror system
Focus characterization of the NanoMAX Kirkpatrick–Baez mirror system Open
The focusing and coherence properties of the NanoMAX Kirkpatrick–Baez mirror system at the fourth-generation MAX IV synchrotron in Lund have been characterized. The direct measurement of nano-focused X-ray beams is possible by scanning of …