Markus A. Seeger
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View article: Capturing transient states of heterodimeric ABC transporter TM287/288 by Time-Resolved Small-Angle X-ray Scattering
Capturing transient states of heterodimeric ABC transporter TM287/288 by Time-Resolved Small-Angle X-ray Scattering Open
Structures of the heterodimeric ABC transporter TM287/288 have been previously determined in several states indicating large conformational changes during its reaction cycle. However, for a complete description of the cycle, transient stat…
View article: ESCMID workshop: Artificial intelligence and machine learning in medical microbiology diagnostics
ESCMID workshop: Artificial intelligence and machine learning in medical microbiology diagnostics Open
Rapid advancements in artificial intelligence (AI) and machine learning (ML) offer significant potential to transform medical microbiology diagnostics, improving pathogen identification, antimicrobial susceptibility prediction and outbreak…
View article: Rapid detection and capture of clinical Escherichia coli strains mediated by OmpA-targeting nanobodies
Rapid detection and capture of clinical Escherichia coli strains mediated by OmpA-targeting nanobodies Open
Escherichia coli is a major cause of blood stream and urinary tract infections. Owing to the spread of antimicrobial resistance, it is often treated with an inadequate antibiotic. With the aim to accelerate the diagnostics of this key path…
View article: FcRn-silencing of IL-12Fc prevents toxicity of local IL-12 therapy and prolongs survival in experimental glioblastoma
FcRn-silencing of IL-12Fc prevents toxicity of local IL-12 therapy and prolongs survival in experimental glioblastoma Open
Glioblastoma remains a challenging indication for immunotherapy: the blood-brain barrier hampers accessibility for systemic treatments and the immunosuppressive microenvironment impedes immune attack. Intratumoral therapy with the proinfla…
View article: MprF from <i>Pseudomonas aeruginosa</i> is a promiscuous lipid scramblase with broad substrate specificity
MprF from <i>Pseudomonas aeruginosa</i> is a promiscuous lipid scramblase with broad substrate specificity Open
The multiple peptide resistance factor (MprF) is a bifunctional membrane protein found in many bacteria, including Pseudomonas aeruginosa and Staphylococcus aureus . MprF modifies inner leaflet lipid headgroups through aminoacylation and t…
View article: Flycodes enable simultaneous preclinical analysis for dozens of antibodies in single cassette–dosed mice
Flycodes enable simultaneous preclinical analysis for dozens of antibodies in single cassette–dosed mice Open
Protein therapeutics such as antibodies require in-depth in vivo characterization during development and consequently account for a large proportion of laboratory animal consumption in the pharmaceutical industry. Currently, antibody candi…
Structural basis for triacylglyceride extraction from mycobacterial inner membrane by MFS transporter Rv1410 Open
Mycobacterium tuberculosis is protected from antibiotic therapy by a multi-layered hydrophobic cell envelope. Major facilitator superfamily (MFS) transporter Rv1410 and the periplasmic lipoprotein LprG are involved in transport of triacylg…
View article: <i>In vitro</i> and <i>ex vivo</i> proteomics of <i>Mycobacterium marinum</i> biofilms and the development of biofilm-binding synthetic nanobodies
<i>In vitro</i> and <i>ex vivo</i> proteomics of <i>Mycobacterium marinum</i> biofilms and the development of biofilm-binding synthetic nanobodies Open
The antibiotic-tolerant biofilms present in tuberculous granulomas add an additional layer of complexity when treating mycobacterial infections, including tuberculosis (TB). For a more efficient treatment of TB, the biofilm forms of mycoba…
View article: Structural basis for triacylglyceride extraction from mycobacterial inner membrane by MFS transporter Rv1410
Structural basis for triacylglyceride extraction from mycobacterial inner membrane by MFS transporter Rv1410 Open
Mycobacterium tuberculosis is protected from antibiotic therapy by a multi-layered hydrophobic cell envelope. Major facilitator superfamily (MFS) transporter Rv1410 and the periplasmic lipoprotein LprG are involved in transport of triacylg…
View article: The ABC transporter MsbA adopts the wide inward-open conformation in <i>E. coli</i> cells
The ABC transporter MsbA adopts the wide inward-open conformation in <i>E. coli</i> cells Open
Membrane proteins are currently investigated after detergent extraction from native cellular membranes and reconstitution into artificial liposomes or nanodiscs, thereby removing them from their physiological environment. However, to truly…
View article: Proteomic analysis of Mycobacterial Biofilm Matrix and Development of Biofilm-binding Synthetic Nanobodies
Proteomic analysis of Mycobacterial Biofilm Matrix and Development of Biofilm-binding Synthetic Nanobodies Open
Tissue-penetrating nanobodies as drug-delivery vehicles have shown efficacy in treating hard-to-reach and -treat diseases. The present study reports nanobodies with application potential in the treatment of tuberculosis (TB), especially th…
View article: Mechanistic basis of choline import involved in teichoic acids and lipopolysaccharide modification
Mechanistic basis of choline import involved in teichoic acids and lipopolysaccharide modification Open
Phosphocholine molecules decorating bacterial cell wall teichoic acids and outer-membrane lipopolysaccharide have fundamental roles in adhesion to host cells, immune evasion, and persistence. Bacteria carrying the operon that performs phos…
View article: Decision letter: Glutathione binding to the plant AtAtm3 transporter and implications for the conformational coupling of ABC transporters
Decision letter: Glutathione binding to the plant AtAtm3 transporter and implications for the conformational coupling of ABC transporters Open
Article Figures and data Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract The ATP binding casset…
View article: DDRE-48. COMPARTMENT LOCKED IL-12 - INCREASED TISSUE RETENTION AND MINIMAL PERIPHERAL EXPOSURE ALLOW HIGHER TREATMENT EFFICACY AND TOLERABILITY IN LOCAL GLIOBLASTOMA THERAPY
DDRE-48. COMPARTMENT LOCKED IL-12 - INCREASED TISSUE RETENTION AND MINIMAL PERIPHERAL EXPOSURE ALLOW HIGHER TREATMENT EFFICACY AND TOLERABILITY IN LOCAL GLIOBLASTOMA THERAPY Open
Recent clinical studies in glioblastoma (GBM) highlight the potential of local IL-12 therapy, but they also bring back tolerability concerns due to leakage into the periphery. This leakage might thus hamper exploiting the full potential of…
View article: Critical discussion on drug efflux in <i>Mycobacterium tuberculosis</i>
Critical discussion on drug efflux in <i>Mycobacterium tuberculosis</i> Open
Mycobacterium tuberculosis (Mtb) can withstand months of antibiotic treatment. An important goal of tuberculosis research is to shorten the treatment to reduce the burden on patients, increase adherence to the drug regimen and thereby slow…
View article: Deep mutational scan of a drug efflux pump reveals its structure-function landscape
Deep mutational scan of a drug efflux pump reveals its structure-function landscape Open
Drug efflux is a common resistance mechanism found in bacteria and cancer cells. Although several structures of drug efflux pumps are available, they provide only limited functional information on the phenomenon of drug efflux. Here, we pe…
View article: Mechanistic basis of choline import involved in teichoic acids and lipopolysaccharide modification
Mechanistic basis of choline import involved in teichoic acids and lipopolysaccharide modification Open
Phosphocholine molecules decorating bacterial cell wall teichoic acids and outer-membrane lipopolysaccharide have significant roles in adhesion to host cells, immune evasion, and persistence. Bacteria carrying the operon that performs phos…
Allosteric modulation of LRRC8 channels by targeting their cytoplasmic domains Open
Members of the LRRC8 family form heteromeric assemblies, which function as volume-regulated anion channels. These modular proteins consist of a transmembrane pore and cytoplasmic leucine-rich repeat (LRR) domains. Despite their known molec…