Explanipedia

Leukocytes use endothelial membrane tunnels to extravasate the vasculature Open

Werner J. van der Meer, Abraham C.I. van Steen, Eike K. Mahlandt, Loïc Rolas, Haitao Wang , et al. · 2025

Upon inflammation, leukocytes extravasate through endothelial cells. When they extravasate, it is generally accepted that neighboring endothelial cells disconnect. Careful examination of endothelial junctions showed a partial membrane over…

Leukocytes use endothelial membrane tunnels to extravasate the vasculature Open

Werner J. van der Meer, Abraham C.I. van Steen, Eike K. Mahlandt, Loïc Rolas, Haitao Wang , et al. · 2024

Upon inflammation, leukocytes extravasate through endothelial cells. When they extravasate in a paracellular manner, it is generally accepted that neighbouring endothelial cells physically disconnect to open cell-cell junctions, allowing l…

Primary adhered neutrophils increase JNK1-MARCKSL1-mediated filopodia to promote secondary neutrophil transmigration Open

Max Laurens Bastiaan Grönloh, Janine J. G. Arts, Eike K. Mahlandt, Martijn A. Nolte, Joachim Goedhart , et al. · 2023

Primary Neutrophil Adhesion Triggers Endothelial Hotspots by Increasing JNK1-MARCKSL1-Mediated Filopodia to Promote Secondary Neutrophil Transmigration Open

Max L.B. Grönloh, Janine J. G. Arts, Eike K. Mahlandt, Martijn A. Nolte, Joachim Goedhart , et al. · 2023

Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM‐1 Open

Max L.B. Grönloh, Janine Arts, Sebastián Palacios Martínez, Amerens A. van der Veen, Lanette Kempers , et al. · 2022

Code Red in the Supply Center: The Impact of Immune Activation on Hematopoiesis Open

Katherine C. MacNamara, Martijn A. Nolte · 2022

This Special Issue entitled “The Impact of Immune Activation on Hematopoiesis” aims to bring together review and primary articles focused on distinct types of immune activation that impact hematopoiesis [...]

Polyfunctional pathogen-specific CD4 + T cells reside in the lungs and tumors of NSCLC patients Open

Anna E. Oja, Florencia Morgana, Ruth R. Hagen, Cherien A. Ghandour, Martijn A. Nolte , et al. · 2022

Local T cell responses are required for optimal protection of the lungs against airborne pathogens. This localized protection is mediated by various immune cells, including resident memory T cells (T RM ). While human lung CD8 + T RM line …

Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM1 Open

Max L.B. Grönloh, Janine Arts, Sebastián Palacios Martínez, Amerens A. van der Veen, Lanette Kempers , et al. · 2022

Upon inflammation, leukocytes leave the circulation by crossing the endothelial monolayer at specific transmigration ‘hotspot’ regions. Although these regions support leukocyte transmigration, their functionality is not clear. We found tha…

The endothelial diapedesis synapse regulates transcellular migration of human T lymphocytes in a CX3CL1- and SNAP23-dependent manner Open

Rouven Schoppmeyer, Abraham C.I. van Steen, Lanette Kempers, Anne L. Timmerman, Martijn A. Nolte , et al. · 2022

Understanding how cytotoxic T lymphocytes (CTLs) efficiently leave the circulation to target cancer cells or contribute to inflammation is of high medical interest. Here, we demonstrate that human central memory CTLs cross the endothelium …

Bone Marrow Harbors a Unique Population of Dendritic Cells with the Potential to Boost Neutrophil Formation upon Exposure to Fungal Antigen Open

Marieke Goedhart, Edith Slot, María Fernanda Pascutti, Sulima Geerman, Timo Rademakers , et al. · 2021

Apart from controlling hematopoiesis, the bone marrow (BM) also serves as a secondary lymphoid organ, as it can induce naïve T cell priming by resident dendritic cells (DC). When analyzing DCs in murine BM, we uncovered that they are local…

Age-Related Variation in Sympathetic Nerve Distribution in the Human Spleen Open

Cindy G. J. Cleypool, David J. Brinkman, Claire Mackaaij, Peter G. J. Nikkels, Martijn A. Nolte , et al. · 2021

Introduction: The cholinergic anti-inflammatory pathway (CAIP) has been proposed as an efferent neural pathway dampening the systemic inflammatory response via the spleen. The CAIP activates the splenic neural plexus and a subsequent serie…

Transendothelial migration induces differential migration dynamics of leukocytes in tissue matrix Open

Abraham C.I. van Steen, Lanette Kempers, Rouven Schoppmeyer, Max Blokker, David J. Beebe , et al. · 2021

Leukocyte extravasation into inflamed tissue is a complex process that is difficult to capture as a whole in vitro. We employed a blood-vessel-on-a-chip model in which human endothelial cells were cultured in a tube-like lumen in a collage…

The RhoGEF Trio: A Protein with a Wide Range of Functions in the Vascular Endothelium Open

Lanette Kempers, Amber J. M. Driessen, Jos van Rijssel, Martijn A. Nolte, Jaap D. van Buul · 2021

Many cellular processes are controlled by small GTPases, which can be activated by guanine nucleotide exchange factors (GEFs). The RhoGEF Trio contains two GEF domains that differentially activate the small GTPases such as Rac1/RhoG and Rh…

Transendothelial migration induces differential migration dynamics of leukocytes in tissue matrix Open

Abraham C.I. van Steen, Lanette Kempers, Rouven Schoppmeyer, Max Blokker, David J. Beebe , et al. · 2021

Leukocyte extravasation into inflamed tissue is a complex process that is difficult to capture as a whole in vitro . We employed a blood-vessel-on-a-chip model in which endothelial cells were cultured in a tube-like lumen in a collagen-1 m…

Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration Open

Janine Arts, Eike K. Mahlandt, Max L.B. Grönloh, Lilian Schimmel, Ivar Noordstra , et al. · 2021

Upon inflammation, leukocytes rapidly transmigrate across the endothelium to enter the inflamed tissue. Evidence accumulates that leukocytes use preferred exit sites, alhough it is not yet clear how these hotspots in the endothelium are de…

Author response: Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration Open

Janine Arts, Eike K. Mahlandt, Max L.B. Grönloh, Lilian Schimmel, Ivar Noordstra , et al. · 2021

The Bone Marrow as Sanctuary for Plasma Cells and Memory T-Cells: Implications for Adaptive Immunity and Vaccinology Open

Stefan Slamanig, Martijn A. Nolte · 2021

The bone marrow (BM) is key to protective immunological memory because it harbors a major fraction of the body’s plasma cells, memory CD4+ and memory CD8+ T-cells. Despite its paramount significance for the human immune system, many aspect…

Extracellular Vesicles Derived From Adult and Fetal Bone Marrow Mesenchymal Stromal Cells Differentially Promote ex vivo Expansion of Hematopoietic Stem and Progenitor Cells Open

Corina A. Ghebes, Jess Morhayim, Marion Kleijer, Merve Koroglu, Stefan J. Erkeland , et al. · 2021

Recently, we and others have illustrated that extracellular vesicles (EVs) have the potential to support hematopoietic stem and progenitor cell (HSPC) expansion; however, the mechanism and processes responsible for the intercellular commun…

Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration Open

Janine Arts, Eike K. Mahlandt, Max L.B. Grönloh, Lilian Schimmel, Ivar Noordstra , et al. · 2021

Upon inflammation, leukocytes rapidly transmigrate across the endothelium to enter the inflamed tissue. Evidence accumulates that leukocytes use preferred exit sites, though it is not yet clear how these hotspots in the endothelium are def…

Integrins Control Vesicular Trafficking; New Tricks for Old Dogs Open

Martijn A. Nolte, Esther N. M. Nolte‐‘t Hoen, Coert Margadant · 2020

Controlling Immunity and Inflammation through Integrin-Dependent Regulation of TGF-β Open

Martijn A. Nolte, Coert Margadant · 2020

GITR shapes humoral immunity by controlling the balance between follicular T helper cells and regulatory T follicular cells Open

Anna E. Oja, Giso Brasser, Edith Slot, René A. W. van Lier, María Fernanda Pascutti , et al. · 2020

Hematopoietic stem and progenitor cells use podosomes to transcellularly cross the bone marrow endothelium Open

Timo Rademakers, Marieke Goedhart, Mark Hoogenboezem, Alexander García Ponce, Jos van Rijssel , et al. · 2020

Bone marrow endothelium plays an important role in the homing of hematopoietic stem and progenitor cells upon transplantation, but surprisingly little is known on how the bone marrow endothelial cells regulate local permeability and hemato…

Activation and suppression of hematopoietic integrins in hemostasis and immunity Open

Martijn A. Nolte, Coert Margadant · 2019

Nolte and Margadant review the current understanding of the activation and inactivation of integrin receptors expressed by hematopoietic cells and the role of these conformational changes in modulating platelet and leukocyte function.

Nuclear shape, protrusive behaviour and in vivo retention of human bone marrow mesenchymal stromal cells is controlled by Lamin-A/C expression Open

Yvonne L. Dorland, Anne S. Cornelissen, Carlijn Kuijk, Simon Tol, Mark Hoogenboezem , et al. · 2019

Culture expanded mesenchymal stromal cells (MSCs) are being extensively studied for therapeutic applications, including treatment of graft-versus-host disease, osteogenesis imperfecta and for enhancing engraftment of hematopoietic stem cel…

Peripheral and systemic antigens elicit an expandable pool of resident memory CD8 + T cells in the bone marrow Open

María Fernanda Pascutti, Sulima Geerman, Nicholas Collins, Giso Brasser, Benjamin Nota , et al. · 2019

BM has been put forward as a major reservoir for memory CD8 + T cells. In order to fulfill that function, BM should “store” memory CD8 + T cells, which in biological terms would require these “stored” memory cells to be in disequilibrium w…

P158/O07 Pathological inflammation in experimental spondyloarthritis is characterized by high endothelial venules and ectopic lymphoid structures in the bone marrow Open

MH Kaaij, JP van Hamburg, Jasper Rip, Γεώργιος Κόλλιας, D. Baeten , et al. · 2019

CXCR4, but not CXCR3, drives CD8 + T‐cell entry into and migration through the murine bone marrow Open

Marieke Goedhart, Stephanie Gessel, Robbert van der Voort, Edith Slot, Beth Lucas , et al. · 2019

The BM serves as a blood‐forming organ, but also supports the maintenance and immune surveillance function of many T cells. Yet, in contrast to other organs, little is known about the molecular mechanisms that drive T‐cell migration to and…

Functional Heterogeneity of CD4+ Tumor-Infiltrating Lymphocytes With a Resident Memory Phenotype in NSCLC Open

Anna E. Oja, Berber Piet, David van der Zwan, Hans Blaauwgeers, Mark Mensink , et al. · 2018

Resident memory T cells (T_RM) inhabit peripheral tissues and are critical for protection against localized infections. Recently, it has become evident that CD103⁺ T_RM are not only important in combating sec…

Author response for "CXCR4, but not CXCR3, drives CD8 T-cell entry into and migration through the murine bone marrow" Open

Marieke Goedhart, Stephanie Gessel, Robbert van der Voort, Edith Slot, Beth Lucas , et al. · 2018

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