Martin Byrdin
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View article: Impact of triplet state population on GFP‐type fluorescence and photobleaching
Impact of triplet state population on GFP‐type fluorescence and photobleaching Open
Background Information Based on recently published parameters (Rane et al. 2023, JPCB 127, 5046–5054) for (rs)EGFP triplet state formation and decay rates and yields, we consider the power density dependence of triplet state population dyn…
View article: Crystal structure of a bacterial photoactivated adenylate cyclase determined by serial femtosecond and serial synchrotron crystallography
Crystal structure of a bacterial photoactivated adenylate cyclase determined by serial femtosecond and serial synchrotron crystallography Open
OaPAC is a recently discovered blue-light-using flavin adenosine dinucleotide (BLUF) photoactivated adenylate cyclase from the cyanobacterium Oscillatoria acuminata that uses adenosine triphosphate and translates the light signal into the …
View article: Crystal structure of a bacterial photoactivated adenylate cyclase determined at room temperature by serial femtosecond crystallography
Crystal structure of a bacterial photoactivated adenylate cyclase determined at room temperature by serial femtosecond crystallography Open
OaPAC is a recently discovered b lue-light u sing flavin adenosine dinucleotide (BLUF) photoactivated adenylate cyclase from the cyanobacterium Oscillatoria acuminata that uses adenosine triphosphate and translates the light signal into th…
View article: Impact of Triplet State Population on GFP-type Fluorescence and Photobleaching
Impact of Triplet State Population on GFP-type Fluorescence and Photobleaching Open
Based on recently published parameters (Rane et al. 2023, JPCB 127, 5046-5054) for (rs)EGFP triplet state formation and decay rates and yields, we consider the power density dependence of triplet state population dynamics and its consequen…
View article: The TR-<i>ic</i>OS setup at the ESRF: time-resolved microsecond UV–Vis absorption spectroscopy on protein crystals
The TR-<i>ic</i>OS setup at the ESRF: time-resolved microsecond UV–Vis absorption spectroscopy on protein crystals Open
The technique of time-resolved macromolecular crystallography (TR-MX) has recently been rejuvenated at synchrotrons, resulting in the design of dedicated beamlines. Using pump–probe schemes, this should make the mechanistic study of photoa…
View article: Photophysical Studies at Cryogenic Temperature Reveal a Novel Photoswitching Mechanism of rsEGFP2
Photophysical Studies at Cryogenic Temperature Reveal a Novel Photoswitching Mechanism of rsEGFP2 Open
Single-molecule localization microscopy (SMLM) at cryogenic temperature opens new avenues to investigate intact biological samples at the nanoscale and perform cryo-correlative studies. Genetically encoded fluorescent proteins (FPs) are ma…
View article: Light-Induced Forward and Reverse Intersystem Crossing in Green Fluorescent Proteins at Cryogenic Temperatures
Light-Induced Forward and Reverse Intersystem Crossing in Green Fluorescent Proteins at Cryogenic Temperatures Open
Combining fluorescence and phosphorescence kinetics, we characterize forward and reverse intersystem crossing (FISC and RISC, respectively) between the singlet and triplet manifolds S ↔ T in photoswitchable (rsEGFP2) and non-photoswitchabl…
View article: Cover Feature: Rational Control of Off‐State Heterogeneity in a Photoswitchable Fluorescent Protein Provides Switching Contrast Enhancement (ChemPhysChem 19/2022)
Cover Feature: Rational Control of Off‐State Heterogeneity in a Photoswitchable Fluorescent Protein Provides Switching Contrast Enhancement (ChemPhysChem 19/2022) Open
The Cover Feature displays a comparison of chromophore conformations in the X-ray crystal structures of the reversibly photoswitchable fluorescent protein rsEGFP2 in its on state (green) and its V151L (purple) and V151A (yellow) variants i…
View article: Photophysical studies at cryogenic temperature reveal a novel photoswitching mechanism of rsEGFP2
Photophysical studies at cryogenic temperature reveal a novel photoswitching mechanism of rsEGFP2 Open
Single-molecule-localization-microscopy (SMLM) at cryogenic temperature opens new avenues to investigate intact biological samples at the nanoscale and perform cryo-correlative studies. Genetically encoded fluorescent proteins (FPs) are ma…
View article: Rational Control of Off‐State Heterogeneity in a Photoswitchable Fluorescent Protein Provides Switching Contrast Enhancement**
Rational Control of Off‐State Heterogeneity in a Photoswitchable Fluorescent Protein Provides Switching Contrast Enhancement** Open
Reversibly photoswitchable fluorescent proteins are essential markers for advanced biological imaging, and optimization of their photophysical properties underlies improved performance and novel applications. Here we establish a link betwe…
View article: Correction to: Pushing the limits of flash photolysis to unravel the secrets of biological electron and proton transfer
Correction to: Pushing the limits of flash photolysis to unravel the secrets of biological electron and proton transfer Open
View article: Pushing the limits of flash photolysis to unravel the secrets of biological electron and proton transfer
Pushing the limits of flash photolysis to unravel the secrets of biological electron and proton transfer Open
View article: Rational control of structural off-state heterogeneity in a photoswitchable fluorescent protein provides switching contrast enhancement
Rational control of structural off-state heterogeneity in a photoswitchable fluorescent protein provides switching contrast enhancement Open
Reversibly photoswitchable fluorescent proteins are essential markers for advanced biological imaging, and optimization of their photophysical properties underlies improved performance and novel applications. Here we establish a link betwe…
View article: Mechanism and dynamics of fatty acid photodecarboxylase
Mechanism and dynamics of fatty acid photodecarboxylase Open
Light makes light work of fatty acids Photosynthetic organisms are notable for their ability to capture light energy and use it to power biosynthesis. Some algae have gone a step beyond photosynthesis and can use light to initiate enzymati…
View article: Mechanistic Investigations of Green mEos4b Reveal a Dynamic Long-Lived Dark State
Mechanistic Investigations of Green mEos4b Reveal a Dynamic Long-Lived Dark State Open
Green-to-red photoconvertible fluorescent proteins (PCFPs) are key players in advanced microscopy schemes such as photoactivated localization microscopy (PALM). Whereas photoconversion and red-state blinking in PCFPs have been studied inte…
View article: Photoswitching mechanism of a fluorescent protein revealed by time-resolved crystallography and transient absorption spectroscopy
Photoswitching mechanism of a fluorescent protein revealed by time-resolved crystallography and transient absorption spectroscopy Open
View article: NMR Reveals Light-Induced Changes in the Dynamics of a Photoswitchable Fluorescent Protein
NMR Reveals Light-Induced Changes in the Dynamics of a Photoswitchable Fluorescent Protein Open
View article: NMR reveals light-induced changes in the dynamics of a photoswitchable fluorescent protein
NMR reveals light-induced changes in the dynamics of a photoswitchable fluorescent protein Open
The availability of fluorescent proteins with distinct phototransformation properties is crucial for a wide range of applications in advanced fluorescence microscopy and biotechnology. To rationally design new variants optimized for specif…
View article: Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM
Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM Open
Green-to-red photoconvertible fluorescent proteins repeatedly enter dark states, causing interrupted tracks in single-particle-tracking localization microscopy (sptPALM). We identified a long-lived dark state in photoconverted mEos4b that …
View article: Trapping a long-lived dark state in photoconvertible fluorescent protein mEos4b
Trapping a long-lived dark state in photoconvertible fluorescent protein mEos4b Open
The dynamic behaviour of fluorescent labels plays a key role in advanced fluorescence imaging techniques.For example, superresolution fluorescence microscopy techniques, which provide access to the visualization of subcellular structures a…
View article: Chromophore twisting in the excited state of a photoswitchable fluorescent protein captured by time-resolved serial femtosecond crystallography
Chromophore twisting in the excited state of a photoswitchable fluorescent protein captured by time-resolved serial femtosecond crystallography Open
View article: Time-resolved serial femtosecond crystallography on photoswitchable fluorescent proteins
Time-resolved serial femtosecond crystallography on photoswitchable fluorescent proteins Open